DYNAMICS OF PHOTOSYNTHETIC AND OXIDATIVE STRESS PARAMETERS OF TWO SPINACH SPECIES AFTER SHORT-TERM LOW UV-B RADIATION EFFECT
This work aimed to underline the dynamics of photosynthetic and oxidative stress parameters of ‘Matador’ and ‘Andromeda’ spinach species after short-term 1 and 2 kJm–2 UV-B radiation effect. When plants reached 3–4 leaves growths stage, the exposure to 1 kJm–2 and 2 kJm–2 UV-B radiation was done once for 68 and 136 minutes, respectively. The photosynthetic and oxidative stress parameters were determined 2, 24, 48 and 72 hours after exposure. The stimulating effect of UV-B emerged on the 3rd day after exposure. The positive effect of UV-B was more pronounced for ‘Matador’. The highest DPPH radical-scavenging capacity and the highest concentration of α-tocopherols were detected 24 hours after 2 kJ UV-B exposure, but the decrease in photosynthetic rate was the highest as well. Meanwhile, on the 3rd day after 1 kJ UV-B exposure, the indicators of oxidative stress of ‘Matador’ decreased, and the photosynthetic rate increased. This study highlights that low UV-B radiation acts as an eustress, by awaking positive changes in photosynthetic and oxidative stress parameters of spinach.
antioxidants; eustress; spinach; photosynthetic parameters; UV-B
Ballare, C.L., Caldwell, M.M., Flint, S.D., Robinson, S.A., Bornman, J.F. (2011). Effects of solar ultraviolet radiation on terrestrial ecosystems. Patterns, mechanisms, and interactions with climate change. Photochem. Photobiol. Sci., 10(2), 226– 241.
Bornman, J.F., Barnes, P.W., Robinson, S.A., Ballare, C.L., Flint, S.D., Caldwell, M.M. (2015). Solar ultraviolet radiation and ozone depletion-driven climate change: effects on terrestrial ecosystems. Photochem. Photobiol. Sci., 14(1), 88–107.
Favory, J.J., Stec, A., Gruber, H., Rizzini, L., Oravecz, A., Funk, M., Albert, A., Cloix, C., Jenkins, G.I., Oakeley, E.J., Seidlitz, H.K., Nagy, F., Ulm, R. (2009). Interaction of COP1 and UVR8 regulates UV‐B‐induced photomorphogenesis and stress acclimation in Arabidopsis. EMBO J., 28(5), 591–601.
Fernandez-Orozco, R., Zielinski, H., Piskula, M.K. (2003). Contribution of low-molecular-weight antioxidants to the antioxidant capacity of raw and processed lentil seeds. Nahrung Food, 47(5), 291–299.
Gill, S.S., Tuteja, N. (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Phys. Biochem., 48(12), 909–930.
Hectors, K., Prinsen, E., De Coen, W., Jansen, M.A., Guisez, Y. (2007). Arabidopsis thaliana plants acclimated to low dose rates of ultraviolet B radiation show specific changes in morphology and gene expression in the absence of stress symptoms. New Phytol., 175(2), 255–270.
Hideg, E., Jansen, M.A., Strid, A. (2013). UV-B exposure, ROS, and stress: inseparable companions or loosely linked associates? Trends Plant Sci., 18(2), 107–115.
Jansen, M.A., Coffey, A.M., Prinsen, E. (2012). UV-B induced morphogenesis: four players or a quartet? Plant Signal. Behav., 7(9), 1185–1187.
Jansen, M.A., Hectors, K., O’Brien, N.M., Guisez, Y., Potters, G. (2008). Plant stress and human health: Do human consumers benefit from UV-B acclimated crops? Plant Sci., 175(4), 449–458.
Jansen, M.A.K., Le Martret, B., Koornneef, M. (2010). Variations in constitutive and inducible UV-B tolerance; dissecting photosystem II protection in Arabidopsis thaliana accessions. Physiol. Plant., 138, 22–34.
Januskaitiene, I. (2011). Effects of substrate acidity and UV-B radiation on photosynthesis of radishes. Open Life Sci., 6(4), 624–631.
Jenkins, G.I. (2009). Signal transduction in responses to UV-B radiation. Ann. Rev. Plant Biol., 60, 407–431.
Kataria, S., Jajoo, A. Guruprasad, K.N. (2014). Impact of increasing Ultraviolet-B (UV-B) radiation on photosynthetic processes. J. Photochem. Photobiol. B Biol., 137, 55–66.
Kranner, I., Minibayeva, F.V., Beckett, R.P., Seal, C.E. (2010). What is stress? Concepts, definitions and applications in seed science. New Phytol., 188(3), 655–673.
Kumari, R., Singh, S., Agrawal, S.B. (2009). Combined effects of Psoralens and ultraviolet-B on growth, pigmentation and biochemical parameters of Abelmoschus esculentus L. Ecotoxicol. Environ. Saf., 72(4), 1129–1136.
Lu, Y., Duan, B., Zhang, X., Korpelainen, H., Berninger, F., Li, C. (2009). Intraspecific variation in drought response of Populus cathayana grown under ambient and enhanced UV-B radiation. Ann. For. Sci., 66(6), 1–12.
Ragaee, S., Abdel-Aal, E.S.M., Noaman, M. (2006). Antioxidant activity and nutrient composition of selected cereals for food use. Food Chem., 98(1), 32–38.
Reddy, K.R., Singh, S.K., Koti, S., Kakani, V.G., Zhao, D., Gao, W., Reddy, V.R. (2013). Quantifying corn growth and physiological responses to ultraviolet-B radiation for modeling. Agron. J., 105(5), 1367–1377.
Sakalauskiene, S., Januskaitiene, I., Juknys, R., Miliauskiene, J. (2013). The effect of UV-B radiation on phytochemical properties of Spinacia oleracea. Rural development 2013: the 6th international scientific conference. Proceedings, 6(2), 227–231.
Schreiner, M., Mewis, I., Huyskens-Keil, S., Jansen, M.A.K., Zrenner, R., Winkler, J.B., O’Brien, N., Krumbein, A. (2012). UV-B-induced secondary plant metabolites-potential benefits for plant and human health. Crit. Rev. Plant Sci., 31(3), 229–240.
Thomas, D., Puthur, J.T. (2017). UV radiation priming: A means of amplifying the inherent potential for abiotic stress tolerance in crop plants. Environ. Exp. Bot., 138, 57–66.
Yu, G.H., Li, W., Yuan, Z.Y., Cui, H.Y., Lv, C.G., Gao, Z.P., Han, B., Gong, Y.Z., Chen, G.X. (2013). The effects of enhanced UV-B radiation on photosynthetic and biochemical activities in super-high-yield hybrid rice Liangyoupeijiu at the reproductive stage. Photosynthetica, 51(1), 33–44.
Wu, D., Hu, Q., Yan, Z., Chen, W., Yan, C., Huang, X., Zhang, J., Yang, P., Deng, H., Wang, J., Deng, X.W., Shi, Y. (2012). Structural basis of ultraviolet-B perception by UVR8. Nature, 484(7393), 214–219.
Zhu, P., Yang, L. (2015). Ambient UV-B radiation inhibits the growth and physiology of Brassica napus L. on the Qinghai-Tibetan plateau. Field Crops Res., 171, 79–85.
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