Anna Kapczyńska

Department of Ornamental Plants, University of Agriculture in Kraków, 29 listopada 54, 31-425 Kraków, Poland


Lachenalia is a poorly known genus of ornamental bulbous plants from South Africa with a huge floricultural potential. The paper discusses in vivo multiplication with leaf cuttings of Lachenalia cv. ‘Namakwa’, ‘Romaud’, ‘Ronina’ and ‘Rupert’. Mature leaves were collected from non-flowering donor plants. Each leaf was halved to make proximal and distal leaf cuttings. ‘Ronina’ distal leaf cuttings produced the lowest number of bulblets (5.7 per cutting), while ‘Namakwa’ proximal leaf cuttings turned out the most productive (10.5 bulblets per cutting). The bulblets produced by cv. ‘Namakwa’ distal leaf cuttings were the lightest (0.3 g per bulblet), and those formed on cv. ‘Ronina’ proximal leaf cuttings were the heaviest (0.9 g per bulblet). In general, the weight of individual bulblets ranged from 0.1 to 1.2 g. Irrespective of the cutting type, the leaves of cv. ‘Namakwa’ produced the greatest number of bulblets, and the leaves of ‘Ronina’ yielded the heaviest bulblets. With no dependence on their genotype, proximal leaf cuttings were advantageous in terms of total number of generated bulblets and their quality (weight, diameter).


bulblet formation, reproduction, Cape Hyacinth, ‘Namakwa’, ‘Romaud’, ‘Ronina’, ‘Rupert’

Bach, A., Kapczyńska, A., Dziurka, K., Dziurka, M. (2015). Phenolic compounds and carbohydrates in relation to bulb formation in Lachenalia ‘Ronina’ and ‘Rupert’ in vitro cultures under different lighting environments. Sci. Hortic., 188, 23–29, DOI: 10.1016/j.scienta.2015.02.038 0304-4238.

Blomerus, L.M., Schreuder, H.A. (2002). Rapid propagation of Ornithogalum using leaf cuttings. Acta Hortic., 570, 293–296. DOI: 10.17660/ActaHortic.2002.570.38.

Bouchabké, O., Tardieu, F., Simonneau, T. (2006). Leaf growth and turgor in growing cells of maize (Zea mays L.) respond to evaporative demand under moderate irrigation but not in water-saturated soil. Plant Cell Environ., 29, 1138–1148. DOI: 10.1111/j.1365-3040.2005.01494.x.

Duncan, G.D. (2012). The Genus Lachenalia. Botanical magazine monograph. Kew Publishing, Kew, 479 pp.

Gorelick, R. (2015). Why vegetative propagation of leaf cuttings is possible in succulent and semi-succulent plants. Haseltonia, 20, 51–57. DOI: 10.2985/026.020.0109.

Kapczyńska, A. (2013). Effect of plant spacing on the growth, flowering and bulb production of four lachenalia cultivars. S. Afr. J. Bot., 88, 164–169. DOI: 10.1016/j.sajb.2013.07.015.

Kapczyńska, A. (2014a). Effect of planting term on growth and flowering of two cultivars of lachenalia produced in a greenhouse as potting plants during winter months. J. Hortic. Res., 22, 29–34. DOI: 10.2478/johr-2014-0003.

Kapczyńska, A. (2014b). Effect of bulb size on growth, flowering and bulb formation in lachenalia cultivars. Hort. Sci. (Prague), 41(2), 89–94.

Kapczyńska, A., Kidawska, A. (2016). ‘Namakwa’ lachenalia’s response to flurprimidol and different planting dates. Folia Hort., 28(2), 173–179. DOI: 10.1515/fhort-2016-0020.

Kapczyńska, A., Malik, M. (2016). Planting time and flurprimidol treatment influence the growth and flowering of lachenalia. HortTechnology, 26(3), 293–298.

Kleynhans, R., Niederwieser, J.G., Hancke, F.L. (2002). Lachenalia: development and commercialisation of a new flower bulb crop. Acta Hortic., 570, 81–85. DOI: 10.17660/ActaHortic.2002.570.7.

Kleynhans, R. (2006). Lachenalia spp. In: Flower breeding and genetics: issues, challenges, and opportunities for the 21st century, Anderson, N.O. (ed.). Springer, Dordrecht, 491–516.

Knippels, P.J.M. (2012). Advanced in vivo propagation techniques for speciality bulbs. Floricult. Ornam. Biotechnol., 6(2), 154–157.

Krause, J. (1980). Propagation of hyacinth by leaf cuttings. Histological changes in leaf cuttings of Hyacinthus orientalis. Acta Hortic., 109, 271–278. DOI: 10.17660/ActaHortic.1980.109.38.

Meng, J., Xu T., Wang, Z., Fang, Y., Xi, Z., Zhang, Z. (2014). The ameliorative effects of exogenous melatonin on grape cuttings under water-deficient stress: antioxidant metabolites, leaf anatomy, and chloroplast morphology. Pineal Res., 57(2), 200–212. DOI: 10.1111/jpi.12159.

Ndou, A.M., Niederwieser, J.G., Robbertse, P.J. (2002). Effect of leaf-section position and physiological stage of the donor plant on Lachenalia leaf-cutting performance. S. Afr. J. Plant Soil, 19(4), 178–181. DOI: 10.1080/02571862.2002.10634461.

Ndou, A.M. (2000). Optimization of bulblet production by leaf cuttings in Lachenalia. PhD dissertation, Faculty of Natural and Agricultural Science, University of Pretoria, Pretoria, South Africa, 89 pp.

Niederwieser, J.G., Vcelar, B.M. (1990). Regeneration of Lachenalia species from leaf explants. HortScience, 25(6), 684–687.

Niederwieser, J.G., Ndou, A.M. (2002). Review on adventitious bud formation in Lachenalia. Acta Hortic., 570, 135–139. DOI: 10.17660/ActaHortic.2002.570.14.

Nndwambi, D., Matsiliza-Mlathi, B., Kleynhans, R. (2013). Optimizing in vivo bulblet formation on leaf cuttings in Eucomis spp. S. Afr. J. Bot., 86, 179, DOI:10.1016/j.sajb.2013.02.151.

Perrignon, R.J. (1992). Bulblet production in vivo from leaves of Lachenalia (Jacq). Acta Hortic., 325, 341–348. DOI: 10.17660/ActaHortic.1992.325.44.

Singh, A. (2015) Micropropagation of plants. In: Plant biology and biotechnology, Bahadur, B., Venkat Rajam, M., Sahijram, L., Krishnamurthy, K. (eds.). Springer, New Delhi, 329–346.

Śmigielska, M., Jerzy, M. (2013). Adventitious roots and bulbs formation on Hyacinthus orientalis leaf cuttings under different colours of artificial light. Acta Sci. Pol. Hortorum Cultus, 12(1), 157–164.

Suh, J.K., Roh, M.S., Lee, J.S. (1997). Leaf cutting propagation, growth and flowering of Lachenalia. Acta Hortic., 430, 369–376. DOI: 10.17660/ActaHortic.1997. 430.57.



Anna Kapczyńska 
Department of Ornamental Plants, University of Agriculture in Kraków, 29 listopada 54, 31-425 Kraków, Poland



Articles are made available under the conditions CC BY 4.0 (until 2020 under the conditions CC BY-NC-ND 4.0).
Submission of the paper implies that it has not been published previously, that it is not under consideration for publication elsewhere.

The author signs a statement of the originality of the work, the contribution of individuals, and source of funding.


Most read articles by the same author(s)