CALLUS INDUCTION AND ORGANOGENESIS IN VITRO OF CATTLEYA FROM PROTOCORM-LIKE BODIES (PLBs) UNDER DIFFERENT LIGHT CONDITIONS
Teresa Cybularz-Urban
University of Agriculture in KrakowEwa Hanus-Fajerska
University of Agriculture in KrakowAnna Bach
University of Agriculture in KrakowAbstract
In this paper we report the research on the effect of the different light in vitro conditions on growth and development of interspecific hybrid Cattleya intermedia × C.
aurantiaca (Orchidaceae) maintained as stock in vitro shoot culture. Callus was obtained from shoots explants on initiation medium which was composed of MS mineral salts and vitamin set, supplemented with 30 g l-1 sucrose, 2.0 mg dm-3 adenine sulphate, 9.7 mg dm-3 ascorbic acid, with addition of 1.0 μM TDZ, whereas callus maintenance medium was instead supplemented with 4.95 μM BA, and 1 μM NAA. Proliferating protocorm-like bodies (PLBs) of that precious plant material were exposed to the irradiation with monochromatic light characterized by different wavelength. Some interesting lines have been obtained on white, blue, red, far red, and ultraviolet light respectively, which proved to be diversified in the proliferation rates as well as in the morphological and anatomical features.
The light treatment also significantly affected regenerative potential of studied culture. Blue light applied during two subsequent culture passages was proved to be the best option in order to regenerate shoots via PLBs. Nevertheless blue, red and far red irradiation of cultures led to distinctive reduction in the content of chlorophyll and carotenoid pigments, compared with culture irradiated with either white or ultraviolet light.
Keywords:
in vitro culture, Orchidaceae, tissue proliferation, photomorphogenesis, micropropagationReferences
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Da Silva, M.H.M., Debergh, P.C. (1997). The effect of light quality on the morphogenesis of in vitro cultures of Azorina vidalii (Wats.) Feer. Plant Cell Tiss. Organ Cult., 51, 187–193.
Arditti, J. (2008). Micropropagation of orchids. Volume I. Blackwell Publishing, USA, UK, Australia, 300–345.
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Cerny-Koenig, T.A., Faust, J.E., Rajapakse, N.C. (2004). Role of gibberelin A4 and gibberelin biosynthesis inhibitors on flowering and stem elongation in Petunia under modified light environments. HortSci., 40(1), 134–137.
Chakravaty, T.N., Sopory, S.K. (1998). Blue light stimulation and glycolase I activity in callus cultures of Amaranthus paniculatus. Plant Sci., 132, 63–69.
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Cybularz-Urban, T., Hanus-Fajerska, E. (2008). The morphogenetic capability and the viability of regenerants in micropropagated orchid hybrids infected with viral pathogens. Folia Hort., 20/2, 93–102.
Halford, N.G. (2012). Toward two decades of plant biotechnology: successes, failures, and prospects. Food Energy Sec., 1(1), 9–28.
Huan, L.V.T., Takamura, T., Tanaka, M. (2004). Callus formation and plant regeneration through somatic embryo structures in Cymbidium orchid. Plant Sci., 166, 1443–1449.
Ilias, I.F., Rajapaske, N. (2005). The effects of end-of-the-day red and far red light on growth and flowering of Petunia × hybrida ‘Countdown Burgundy’ grown under photoselective films. Hort. Sci., 40(1), 131–133.
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Kozak, D. (1991). Shoot regeneration from various parts of Narcissus cv. Carlton through tissue culture. Prace Instytutu Sadownictwa i Kwiaciarstwa, Rośliny ozdobne − Studies of ISiK, Ornamental Plants 16, 41–47.
Kumar, N., Kumar, S., Ahuja, P.S. (2007). Different photosynthetic response of wild and cultivated plants to high irradiance. Photosynthetica, 45(1), 70–74.
Lavrentyeva, A.M., Ivanikov, R.V. (2007). In vitro propagation of Cattleya Lindl. and Laelia Lindl. species. Lankesteriana, 7(1–2), 147–149.
Lin, Y.H., Chang, C., Chang, W.C. (2000). Plant regeneration from callus culture of a Paphiopedilum hybrid. Cell Tiss Organ Cult., 62, 21–25.
Mudalige, R.G., Kuehnle, A.R. (2004). Orchid biotechnology in production and improvement. HortSci., 39(1), 12–16.
Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15, 473–497.
Nishimura, M.T., Dangl, J.L. (2010). Arabidopsis and the plant immune system. Plant J., 61, 1053–1066.
Peres, L.E.P., Kerbauy, G.B. (1999). High cytokinin accumulation following root tip excision changes the endogenous auxin-to-cytokinin ratio during root-to-shoot conversion in Catasetum fimbriatum Lindl. (Orchidaceae). Plant Cell Rep. 18, 1002–1006.
Petchthai, U., Chuphrom, A., Huehne, P.S. (2015). Recovery of virus-infected Dendrobium orchids by constitutive expression of the Cymbidium mosaic virus coat protein gene. Plant Cell Tiss. Organ Cult., 120, 597–606.
Pindel, A., Miczyński, K. (1996). Regeneration of Cymbidium orchid from leaf and root explants. Folia Hort., 8(2), 95–105.
Prażak, R. (2001). Micropropagation of Dendrobium kingianum Bidwill orchid. Biotechnologia, 2(33), 144–147.
Sarbia-Ochoa, M.E., Avila-Diaz, I., Carlos-Gómez, A., Saldago-Garciglia, R. (2010). Callus growth and plant regeneration in Laelia speciosa (Orchidaceae). Lankesteriana, 10(1), 13–18.
Shin, K.S., Murthy, H.N., Heo, J.W., Hahn, E.J., Paek, K.Y. (2008). The effect of light quality on the growth and development of in vitro cultured Doritaneopsis plants. Acta Physiol. Plant. 30, 339–343.
Tanaka, M., Takamura, T., Watanebe, H., Endo, M., Yanagi, T., Okamoto, K. (1998). In vitro growth of Cymbidium plantlets cultured under super red and blue light emitting diodes (LEDs). J. Hort. Sci. Biotech., 73, 39–44.
Tibbits, T.W., Morgan, D.C., Warrington, J.J. (1983). Growth of lettuce, spinach, mustard and wheat under four combinations of high-pressure sodium metal halide and tungsten halogen lamps. J. Am. Hort. Sci., 108, 662–630.
Wellburn, A.R. (1994). The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J. Plant Physiol., 144(3), 307–313.
Wiszniewska, A., Hanus-Fajerska, E., Smoleń, S., Muszyńska, E. (2015). In vitro selection for lead tolerance in shoot culture of Daphne species. Acta Sci. Pol. Hortorum Cultus, 14(1), 129–142.
Woźny, A. (2011). Use of light to control the growth of Salvia splendens Sellow ex Roem. et Schult. seedlings. Acta Sci. Pol. Hortorum Cultus, 10(4), 99–106.
Yeach, Ch.H., Kaplinsky, N., Hu, C., Charng, Y. (2012). Some like it hot, some like it warm: phenotyping to explorer termotolerance. Plant Sci., 195, 10–23.
Altman, A. (1999). Plant biotechnology in the 21st century: the challenges ahead. Elect. J. Biotech. www.ejb.org/content/vol2/issue2/full/1.
de Arajoújo, A.G., Pasqual, M., Rodrigues, F.A., Rodrigues, J.D., de Castro, E.M., Santos, A.M. (2009). Crescimento in vitro de Cattleya loddigesii Lindl. em diferentes espectros luminosos associados com ácido giberérelico. Rev. Ceres. Viçosa., 56(5), 542–546.
Da Silva, M.H.M., Debergh, P.C. (1997). The effect of light quality on the morphogenesis of in vitro cultures of Azorina vidalii (Wats.) Feer. Plant Cell Tiss. Organ Cult., 51, 187–193.
Arditti, J. (2008). Micropropagation of orchids. Volume I. Blackwell Publishing, USA, UK, Australia, 300–345.
Bach, A., Świderski, A. (2000). The effect of light quality on organogenesis of Hyacinthus orientalis L. in vitro. Acta Biol. Cracov. Series Bot., 42(1), 115–120.
Bach, A., Pawłowska, B. (2006). Effect of light qualities on cultured in vitro ornamental bulbous plants. [In:] Floriculture, ornamental and plant biotechnology. Advances and topical issues, Da Silva J.A.T. (ed.). Global Science Books, Ltd., Japan, 271–276.
Bach, A., Kapczyńska, A., Dziurka, K., Dziurka, M. (2015). Phenolic compounds and carbohydrates in relation to bulb formation in Lachenalia ‘Ronina’ and ‘Rupert’ in vitro cultures under different lighting environments. Sci. Horticult., 188, 23–29.
Batygina, T.B., Bragina, E.A., Vasilieva, V.E. (2003). The reproductive system and germination in orchids. Acta Biol. Cracovien., ser. Bot., 45(2), 21–34.
Cerny-Koenig, T.A., Faust, J.E., Rajapakse, N.C. (2004). Role of gibberelin A4 and gibberelin biosynthesis inhibitors on flowering and stem elongation in Petunia under modified light environments. HortSci., 40(1), 134–137.
Chakravaty, T.N., Sopory, S.K. (1998). Blue light stimulation and glycolase I activity in callus cultures of Amaranthus paniculatus. Plant Sci., 132, 63–69.
Cybularz-Urban, T., Hanus-Fajerska, E. (2006). Therapeutic effect of cytokinin sequence application on virus-infected Cattleya tissue cultures. Acta Biol. Cracov., ser. Bot., 48/2, 27–32.
Cybularz-Urban, T., Hanus-Fajerska, E. (2008). The morphogenetic capability and the viability of regenerants in micropropagated orchid hybrids infected with viral pathogens. Folia Hort., 20/2, 93–102.
Halford, N.G. (2012). Toward two decades of plant biotechnology: successes, failures, and prospects. Food Energy Sec., 1(1), 9–28.
Huan, L.V.T., Takamura, T., Tanaka, M. (2004). Callus formation and plant regeneration through somatic embryo structures in Cymbidium orchid. Plant Sci., 166, 1443–1449.
Ilias, I.F., Rajapaske, N. (2005). The effects of end-of-the-day red and far red light on growth and flowering of Petunia × hybrida ‘Countdown Burgundy’ grown under photoselective films. Hort. Sci., 40(1), 131–133.
Islam, M.O., Matsui, S., Ichihaishu, S. (1999). Effects of light quality on seed germination and seedling growth of Cattleya orchids in vitro. J. Japan. Soc. Hort. Sci., 68(6), 1132–1138.
Islam, M.O., Matsui, S., Ichihaishu, S. (2000). Effects of light quality on carotenoid contents of in vitro growing seedlings of Cattleya. J. Orchid Soc. India, 14(1/2), 7–17.
Karban, R. (2011). The ecology and evolution of induced resistance against herbivores. Funct. Ecol., 25, 339–347.
Kozak, D. (1991). Shoot regeneration from various parts of Narcissus cv. Carlton through tissue culture. Prace Instytutu Sadownictwa i Kwiaciarstwa, Rośliny ozdobne − Studies of ISiK, Ornamental Plants 16, 41–47.
Kumar, N., Kumar, S., Ahuja, P.S. (2007). Different photosynthetic response of wild and cultivated plants to high irradiance. Photosynthetica, 45(1), 70–74.
Lavrentyeva, A.M., Ivanikov, R.V. (2007). In vitro propagation of Cattleya Lindl. and Laelia Lindl. species. Lankesteriana, 7(1–2), 147–149.
Lin, Y.H., Chang, C., Chang, W.C. (2000). Plant regeneration from callus culture of a Paphiopedilum hybrid. Cell Tiss Organ Cult., 62, 21–25.
Mudalige, R.G., Kuehnle, A.R. (2004). Orchid biotechnology in production and improvement. HortSci., 39(1), 12–16.
Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15, 473–497.
Nishimura, M.T., Dangl, J.L. (2010). Arabidopsis and the plant immune system. Plant J., 61, 1053–1066.
Peres, L.E.P., Kerbauy, G.B. (1999). High cytokinin accumulation following root tip excision changes the endogenous auxin-to-cytokinin ratio during root-to-shoot conversion in Catasetum fimbriatum Lindl. (Orchidaceae). Plant Cell Rep. 18, 1002–1006.
Petchthai, U., Chuphrom, A., Huehne, P.S. (2015). Recovery of virus-infected Dendrobium orchids by constitutive expression of the Cymbidium mosaic virus coat protein gene. Plant Cell Tiss. Organ Cult., 120, 597–606.
Pindel, A., Miczyński, K. (1996). Regeneration of Cymbidium orchid from leaf and root explants. Folia Hort., 8(2), 95–105.
Prażak, R. (2001). Micropropagation of Dendrobium kingianum Bidwill orchid. Biotechnologia, 2(33), 144–147.
Sarbia-Ochoa, M.E., Avila-Diaz, I., Carlos-Gómez, A., Saldago-Garciglia, R. (2010). Callus growth and plant regeneration in Laelia speciosa (Orchidaceae). Lankesteriana, 10(1), 13–18.
Shin, K.S., Murthy, H.N., Heo, J.W., Hahn, E.J., Paek, K.Y. (2008). The effect of light quality on the growth and development of in vitro cultured Doritaneopsis plants. Acta Physiol. Plant. 30, 339–343.
Tanaka, M., Takamura, T., Watanebe, H., Endo, M., Yanagi, T., Okamoto, K. (1998). In vitro growth of Cymbidium plantlets cultured under super red and blue light emitting diodes (LEDs). J. Hort. Sci. Biotech., 73, 39–44.
Tibbits, T.W., Morgan, D.C., Warrington, J.J. (1983). Growth of lettuce, spinach, mustard and wheat under four combinations of high-pressure sodium metal halide and tungsten halogen lamps. J. Am. Hort. Sci., 108, 662–630.
Wellburn, A.R. (1994). The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J. Plant Physiol., 144(3), 307–313.
Wiszniewska, A., Hanus-Fajerska, E., Smoleń, S., Muszyńska, E. (2015). In vitro selection for lead tolerance in shoot culture of Daphne species. Acta Sci. Pol. Hortorum Cultus, 14(1), 129–142.
Woźny, A. (2011). Use of light to control the growth of Salvia splendens Sellow ex Roem. et Schult. seedlings. Acta Sci. Pol. Hortorum Cultus, 10(4), 99–106.
Yeach, Ch.H., Kaplinsky, N., Hu, C., Charng, Y. (2012). Some like it hot, some like it warm: phenotyping to explorer termotolerance. Plant Sci., 195, 10–23.
Teresa Cybularz-Urban
University of Agriculture in Krakow
University of Agriculture in Krakow
Ewa Hanus-Fajerska
University of Agriculture in Krakow
University of Agriculture in Krakow
Anna Bach
University of Agriculture in Krakow
University of Agriculture in Krakow
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