IMPROVEMENT OF NUTRACEUTICAL VALUE OF BROCCOLI SPROUTS BY NATURAL ELICITORS
Urszula Gawlik-Dziki
University of Life Sciences in LublinMichał Świeca
University of Life Sciences in LublinDariusz Dziki
University of Life Sciences in LublinDanuta Sugier
University of Life Sciences in LublinAbstract
Contrary to genetic engineering elicitation is a cheaper and socially acceptable methods for improving plant food functionality. In this work broccoli sprouts were elicited with Saccharomyces cerevisiae (SC) and Salix daphnoides bark (SD) extracts. The most effective elicitors of phenolics overproduction were 1% SD and 0.5% SC. Treatment with 0.1% SC significantly increased content of ferulic acid, whereas p-coumaric and syryngic acids levels were significantly elevated by elicitation with SC (0.5% and 1%) and SD. All studied extracts appeared to be very effective elicitors of kaempferol biosynthesis (17-fold for 0.1% SC). In the case of mastication-extractable phytochemicals significant increase of SOD-like activity was observed after elicitation with 0.1% SC, 1% SC and 1% SD. All elicitors caused also an increase of OH• radicals scavenging ability. The most effective was 1% SC, where an increase was about 40%. Elicitation significantly improved potential bioaccessibility of compounds with anti-ROS activities, especially SOD-like active phytochemicals, thus may consist an effective biotechnology.
Keywords:
elicitation, broccoli sprouts, phenolic compounds, OH• radicals, antioxidant activityReferences
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Zabala G., Zou J., Tuteja J., Gonzalez D.O., Clough S.J., Vodkin L.O., 2006. Transcriptome changes in the phenylpropanoid pathway of Glycine max in response to Pseudomonas syringae infection. BMC Plant Biol. 6, 26.
Zhao J., Lawrence C.D., Verpoorte R., 2005. Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol. Adv. 23, 283–333.
Brown A.F., Yousef G.G., Jeffery E.H., Klein B.P., Wallig M.A., Kushad M.M., et al., 2002. Glucosinolate profiles in broccoli: Variation in levels and implications in breeding for cancer chemoprotection. J. Am. Soc. Hortic. Sci. 127, 807–813.
Dziki D., Laskowski J., 2010. Study to analyze the influence of sprouting of the wheat grain on the grinding process. J. Food Eng. 96, 562–567.
Fahey J.W., Kensler T.W., 2007. Role of dietary supplements/nutraceuticals in chemoprevention through induction of cytoprotective enzymes. Chem. Res. Toxicol. 20, 572–576.
Fujita M., Fujita Y., Noutoshi Y., Takahashi F., Narusaka, Y., Yamaguchi-Shinozaki K., Shinozaki, K., 2006. Crosstalk between abiotic and biotic stress responses: a current view from the points of convergence in the stress signaling networks. Curr. Op. Plant Biol. 9, 436–442.
Gawlik-Dziki U., Świeca, M., Sugier D., 2012. Enhancement of antioxidant abilities and the lipoxygenase and xanthine oxidase inhibitory activity of broccoli sprouts by biotic elicitors. Acta Sci. Pol., Hortorum Cultus 11(1), 13–25.
Halliwell B., and Gutteridge J.M.C., 1993. Free radical biology and medicine. 2nd ed. Claredon Press, Oxford.
Halliwell B., Gutteridge J.M.C. and Cross C.E., 1992. Free radicals, antioxidants, and human disease: where are we now? J. Lab. Clin. Med. 119, 598–620.
Jacobs D.R. Gross M.D. and Tapsell L.C., 2009. Food synergy: an operational concept for understanding nutrition. Am. J. Clin. Nutr. 89, 1543–1548.
Jahangir M., Bayoumi Abdel-Farid I., Kim H.K., Choi Y.H., Verpoorte R., 2009. Healthy and unhealthy plants: the effect of stress on the metabolism of Brassicaceae. Environ. Experim. Botany. 67, 23–33.
Lipinski B., 2011. Hydroxyl radical and its scavengers in health and disease. Oxid. Med. Cell. Longev. doi: 10.1155/2011/809696.
Lipton P., 1999. Ischemic cell death in brain neurons. Physiol. Rev. 79, 1431–1568.
Malins D.C., 1993. Identification of hydroxyl radical-induced lesions on DNAnase structure: biomarkers with a putative link to cancer development. J. Toxicol. Environ. Health. 40, 247–261.
Marklund S., Marklund G., 1974. Involvement of the superoxide anion radical in the autooxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 47, 469–474.
Michiels C. 2004. Physiological and pathological responses to hypoxia. Am. J. Pathol. 164, 1875–1882.
Mohammad A., Faruqi F.B., Mustafa J., 2009. Edible compounds as antitumor agents. Indian J. Sci. Technol. 2, 62–74.
Moreno D.A., Lopez-Berenguer C., Martinez-Ballesta M. C., Carvajal M., Garcia-Viguera C., 2008. Basis for the new challenges of growing broccoli for health in hydroponics. J. Sci. Food Agric. 88, 1472–1481.
Pérez-Balibrea S., Moreno D.A., García-Viguera C., 2011a. Genotypic effects on the phytochemical quality of seeds and sprouts from commercial broccoli cultivars. Food Chem. 125, 348–354.
Pérez-Balibrea S., Moreno D.A., García-Viguera C., 2011b. Improving the phytochemical composition of broccoli sprouts by elicitation. Food Chem. 129, 35–44.
Randhir R., Lin Y-T., Shetty K., 2004. Stimulation of phenolics, antioxidant and antimicrobial activities in dark germinated mung bean sprouts in response to peptide and phytochemical elicitors. Process Biochem. 39, 637–646.
Sengul M., Yildiz H., Gungor N., Cetin B., Eser Z. Ercisli S., 2009. Total phenolic content, antioxidant and antimicrobial activities of some medicinal plants. Pak. J. Pharm. Sci. 22, 102–106.
Singh J., Upadhyay A. K., Prasad K., Bahadur A., Rai, M., 2007. Variability of carotenes, vitamin C, E and phenolics in Brassica vegetables. J. Food Compos.Anal. 20, 106–112.
Solecka B., 1997. Role of phenylpropanoid compounds in plant responses to different stress factors. Acta Physiol. Plantarum. 19, 257–268.
Srinivasan M. Sudheer A.R. Menon V.P., 2007. Ferulic acid: therapeutic potential through its antioxidant property. J. Clin. Biochem. Nutr. 40, 92–100.
Su XY., Wanga ZY., Liu, JR., 2009. In vitro and in vivo antioxidant activity of Pinus koraiensis seed extract containing phenolic compounds. Food Chem. 117, 681–686.
Sugier D., Sugier P., Pawelek M., Gawlik-Dziki U., 2011. Salix myrsinifolia Salisb. as a source of phenolic glycosides: distribution and characteristic of habitat conditions in the mid-eastern Poland. Acta Sci. Pol., Hort. Cultus. 10, 75–88.
Świeca M., Gawlik-Dziki U., Kowalczyk D., Złotek U., 2012. Impact of germination time and type of illumination on the antioxidant compounds and antioxidant capacity of Lens culinaris sprouts. Sci. Hortic. 140, 87–95.
Vallejo F., Tomás-Barberán F. A., Ferreres F., 2004. Characterization of flavonols in broccoli (Brassica oleracea L. var. italica) by liquid chromatography-UV diodearray detectionelectrospray ionisation mass spectrometry. J. Chrom. A. 1054, 181–193.
Zabala G., Zou J., Tuteja J., Gonzalez D.O., Clough S.J., Vodkin L.O., 2006. Transcriptome changes in the phenylpropanoid pathway of Glycine max in response to Pseudomonas syringae infection. BMC Plant Biol. 6, 26.
Zhao J., Lawrence C.D., Verpoorte R., 2005. Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol. Adv. 23, 283–333.
Urszula Gawlik-Dziki
University of Life Sciences in Lublin
University of Life Sciences in Lublin
Michał Świeca
University of Life Sciences in Lublin
University of Life Sciences in Lublin
Dariusz Dziki
University of Life Sciences in Lublin
University of Life Sciences in Lublin
Danuta Sugier
University of Life Sciences in Lublin
University of Life Sciences in Lublin
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