Foreword The team of the editors hands the monograph “Biologically active substances of plant origin in human medicine and veterinary medicine” over to a reader with a view to the performance of exemplary vegetal biologically active substances
Olga V. Mushkina
Belarusian State Medical University, Minsk, Republic of BelarusNatalia S. Gurina
Belarusian State Medical University, Minsk, Republic of BelarusMira M. Konopleva
Vitebsk State Medical University, Vitebsk, Republic of BelarusWiesława Byłka
Poznań University of Medical Sciences, Poznań, PolandIrena Matlawska
Poznań University of Medical Sciences, Poznań, PolandAbstract
Alnus glutinosa and A. incana (Betulaceae), are a small to medium size tree, native to the Northern Hemisphere. The leaves of this species are used in the Republic of Belarus as a source of antioxidants. The aim of this work was investigation of antioxidant activities and total phenolics content in various extracts obtained using water, mixture water with ethanol (from 9:1 to 2:8), and ethanol from A. glutinosa and A. incana leaves. Phenolics content was determined by method with Folin-Ciocalteu reagent and calculated on ellagic acid. The antioxidant activities were measured utilising 1,1-diphenyl-2 picrylhydrazyl (DPPH) radical scavenging test. The highest phenolics concentration was observed in the extracts prepared by extraction with the mixture of water:ethanol from 7:3 to 3:7 and ranged from 17.82% to 18.96% for A. glutinosa and from 10.82% to 12.55% for A. incana. This extracts exhibited the highest free radical scavenging activity ranging from 49.21% to 49.42% and from 41.28% to 41.67% for A. glutinosa and A. incana respectively, comparable to the activity of quercetin. Therefore the mixture of water:ethanol from 7:3 to 3:7 should be used for preparing extracts from this species for medicinal purposes. Results also indicated the existence of a high correlation between antioxidant activity and total phenolics content.
Keywords:
leaf extracts, DPPH, hiperoside, phenilic acidsReferences
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Belarusian State Medical University, Minsk, Republic of Belarus
Belarusian State Medical University, Minsk, Republic of Belarus
Vitebsk State Medical University, Vitebsk, Republic of Belarus
Poznań University of Medical Sciences, Poznań, Poland
Poznań University of Medical Sciences, Poznań, Poland
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