MICROPROPAGATION OF SWEET POTATO (Ipomoea batatas (L.) Lam.) FROM NODE EXPLANTS
Romuald Doliński
University of Life Sciences in LublinAnna Olek
University of Life Sciences in LublinAbstract
In the moderate climate, sweet potato plantations are relatively scarce. The plantations are established after spring frosts using well-rooted, hardened cuttings. The
cuttings are obtained from the shoots grown from tubers buried in warm, humid soil or from plants growing in greenhouses. However, this method is inefficient with respect to larger plantation. The aim of the study was to estimate the potential for the production of larger numbers of uniform, well-rooted sweet potato plants by means of in vitro culturing. The study covered two cultivars – ‘Carmen Rubin’ and ‘White Triumph’. The node explants were placed on two growth media containing the basic components of the MS medium as well as growth regulators. The first medium was supplemented with 1 mg dm-3 gibberellin
and 0.1 mg dm-3 kinetin, while the second one – with 0.5 mg dm-3 IAA. The induction of organogenesis and regeneration of the plants took place on the same medium, with no passage. Within 9 weeks, 4 plants were produced from each primary explants in two multiplication cycles. The properties of the plantlets depended on the cultivar, weight of the explants and composition of the medium. The average weight of the ‘Carmen Rubin’ plants was higher than that of the ‘White Triumph’ ones. Moreover, the ‘Carmen Rubin’ plants produced longer shoots and more developed root systems. The sweet potato micro-plants displayed an ability to acclimatize quickly.
Keywords:
in vitro propagation type of medium, properties of plantletsReferences
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University of Life Sciences in Lublin
University of Life Sciences in Lublin
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