IMPACT OF INFECTIONS WITH Trichoderma pleurotum AND Trichoderma pleuroticola ISOLATES ON YIELDING OF WILD STRAINS OF Pleurotus ostreatus (Fr.) Kumm. OBTAINED FROM NATURAL SITES
Krzysztof Sobieralski
Poznań University of Life SciencesMarek Siwulski
Poznań University of Life SciencesLidia Błaszczyk
Institute of Plant Genetics, Polish Academy of Science in PoznańBarbara Frąszczak
Poznań University of Life SciencesIwona Sas-Golak
Poznań University of Life SciencesAbstract
Green moulds of Trichoderma genus cause high losses in oyster mushroom cultivations in many countries. Recently two new species i.e. T. pleurotum and T. pleuroticola have been identified. In the experiment yielding levels of Pleurotus ostreatus growing on substrates infected with Trichoderma isolates were investigated. The following Trichoderma isolates were used: T. pleurotum designated as KWK/17 and T. pleuroticola designated as KMS/21 as well as three wild strains and one cultivated strain of P. ostreatus. It was found that the substrate infections with T. pleurotum and T. pleuroticola isolates caused significant yield reductions of the examined strains of oyster mushrooms. The infection with the T. pleuroticola KMS/21 isolate caused a significantly higher yield drops than the infection with the T. pleurotum KWK/17 isolate in comparison with yields recorded from uninfected substrates.
Keywords:
green moulds, molecular identification, substrate, oyster mushroom, yield reductionReferences
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Chełkowski J., Golka L., Stępień Ł., 2003. Application of STS markers for leaf rust resistance genes in near-isogenic lines of spring wheat cv. Thatcher. J. App. Genet. 44, 323–338.
Doohan F.M., Parry D.W., Jenkinson P., Nicholson P., 1998. The use of species-specific PCR-based assays to analyse Fusarium ear blight of wheat. Plant Pathol. 47, 197–205.
Druzhinina I.S., Kopchinskiy A.G., Komoń M., Bissett J., Szakacs G., Kubicek C.P., 2005. An oligonucleotide barcode for species identification in Trichoderma and Hypocrea. Fungal. Genet. Biol. 42, 813–28.
Frużyńska-Jóźwiak D., Siwulski M., Sobieralski K., Sas-Golak I., Błaszczyk L., 2010. Impact of Trichoderma isolates on the mycelium development of wild strains of Coprinus comatus (Müll.) S.F. Gray J. Plant Protect. Res. 51 (2), 163–166.
Hatvani L., Antal L., Manczinger L., Szekeres A., Druzhinina I.S., Kubicek C.P., Nagy E., Vagvolgyi C., Kredics L., 2007. Green mold diseases of Agaricus and Pleurotus spp. are caused by related but phylogenetically different Trichoderema species. Phytopathology 97 (4), 532–537.
Komon-Zelazowska M., Bisset J., Zafari D., Hatvani L., Manczinger L., Woo S., Lorito M., Kredics L., Kubicek C.P., Druzhinina I.S., 2007. Genetically closely related but phenotypically divergent Trichoderma species cause green mold disease in oyster mushroom farms worldwide. Appl. Environ. Microbiol. 73 (22), 7415–7426.
Kredics L., Hatvani L., Antal L., Manczinger L., Druzhinina I.S., Kubicek C.P., Szekeres A., Nagy A., Vagvolgyi C., Nagy E., 2006. Green mold disease of oyster mushroom in Hungary and Transylvania. Acta Microbiol. Immunol. Hung. 53, 306–307.
Kredics L., Kocsube S., Nagy L., Komon-Zelazowska M., Manczinger L., Sajben E., Nagy A., Vagvolgyi C., Kubicek C.P., Druzhinina I.S., Hatvani L., 2009. Molecular identification of Trichoderma species associated with Pleurotus osteratus and natural substrates of the oyster mushroom. Microbial. Lett. 300, 58–67.
Lemke G., 1971. Mycelenzucht und Fruchtkorperprokuktion des Kulturchampignons Agaricus bisporus (Lange) Sing. Gartenbauwissenschaft 36, 18, 19–27.
Park M.S., Bae K.S., Yu S. H., 2004a. Molecular and morphological analysis of Trichoderma isolates associated with green mold epidemic of oyster mushroom in Korea. J. Huazhong Agric. Univ. 23, 157–164.
Park M.S., Bae K.S., Yu S. H., 2004b. Morphological and molecular of Trichoderma isolates associated with green mold epidemic of oyster mushroom in Korea, www.mushworld.com
Park M.S., Bae K.S., Yu S. H., 2004c. Morphological and molecular of Trichoderma isolates associated with green mold epidemic of oyster mushroom in Korea. New Challenges in Mushroom Science. Proceeding of the 3rd Meeting of Far East Asia for Collaboration of Edible Fungi Research, Suwon, Korea, 143–158.
Park M.S., Bae K.S., Yu S. H., 2006. Two new species of Trichoderma associated with green mold of oyster mushroom cultivation in Korea. Mycobiology 34, 111–113.
Sharma S.R., Vijay B., 1996. Yield loss in Pleurotus ostreatus spp. caused by Trichoderma viride. Mushroom Res. 5, 19–22.
Sobieralski K., Siwulski M., Frużyńska-Jóźwiak D., Górski R., 2009. Impact of Trichoderma aggressivum f. europaeum Th2 on the yielding of Agaricus bisporus. Phytopat. Polonica 53, 5–10.
Sobieralski K., Siwulski M., Jasińska A, Frużyńska-Jóźwiak D., Sas-Golak I., Szymański J., 2010a. Impact of infections with Trichoderma agressivum f. europaeum isolates on the yielding of some wild strains of Agaricus bitorquis (Quel.) Sacc. from different regions of Poland. Phytopat. Polonica 58, 5–11.
Sobieralski K., Siwulski M., Górski R., Frużyńska-Jóźwiak D., Nowak-Sowińska M., 2010b. Impact of Trichoderma aggressivum f. europaeum isolates on yielding and morphological features of Pleurotus eryngii. Phytopatologia Polonica 56, 17–25.
Szekeres A., Kredics L., Antal L., Hatvani L., Manczinger L., Vagvolgyi C., 2005. Genetic diversity of Trichoderma strains isolated from winter wheat rhizosphere in Hungary . Acta Microbiol. Immunol. Hung. 52, 51–56.
Thompson J.D., Higgins D.G., Gibson T.J., 1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucl. Acids. Res. 22, 4673–4680.
Woo S.L., Di Benedetto P., Senator M., Abadi K., Gigante S., Soriente I., Ferraioli S., Scala F., Lorito M., 2004. Identification and characterization of Trichoderma species aggressive to Pleurotus in Italy. J. Zhejiang Univ. Agric. Life Sci. 30, 469–470.
Krzysztof Sobieralski
Poznań University of Life Sciences
Poznań University of Life Sciences
Marek Siwulski
Poznań University of Life Sciences
Poznań University of Life Sciences
Lidia Błaszczyk
Institute of Plant Genetics, Polish Academy of Science in Poznań
Institute of Plant Genetics, Polish Academy of Science in Poznań
Barbara Frąszczak
Poznań University of Life Sciences
Poznań University of Life Sciences
Iwona Sas-Golak
Poznań University of Life Sciences
Poznań University of Life Sciences
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