Ewa Hanus-Fajerska

University of Agriculture in Kraków

Krystyna Ciarkowska

University of Agriculture in Kraków


Solanaceae plants are a source of large number of valuable metabolites with multiple use both in vivo and in vitro conditions. Leaf explants obtained from N. tabacum ‘Samsun’ were cultivated on solidified MS medium supplemented with 3% (w/v) sucrose and enriched with different doses of extracts obtained from seedlings of the same plant cultivar or from N. tabacum ‘Havana’. The aim of experiments was to verify the effectiveness of such medium additives on callus tissue vitality, proliferation and its organogenetic response. It was ascertained a significant positive influence of all kinds of added extracts on increase of callus fresh weight and the organogenetic capability of tobacco cultures. However, the impact of seedlings devoid of roots Nicotiana extracts was significantly
lower in both: stimulating of cell proliferation and shoot formation than extracts obtained from whole seedlings. The biological activity was the most effective in the case of genotype compatibility between the plant material from which the extract was derived with the object of biotest.


plant extracts, tobacco, in vitro cultures, callus proliferation, organogenesis

Arntzen C., 1997. High-tech herbal medicine: plant-based vaccines. Nature Biotechnol. 15, 221–223.
Buiatti M., Ingram D.S., 1991. Phytotoxins as tools in breeding and selection of disease-resistant plants. Cell. Mol. Life Sci. 47(8), 811–819.
Dieryck W., Pagnier J., Poyart C., Marden M.C., Gruber V., Bourant I., Baudino S., Merot B., 1997. Human haemoglobin from transgenic tobacco. Nature 386, 29–30.
Dubert F., Płażek A., 2007. In vitro culture as a model in the studies of physiological processes in plants. Zesz. Probl. Post. Nauk Roln. 523, 93–103.
Gürel E., 2001. Insertation of an antimicrobial gene into Agrobacterium and its further use in transforming tobacco. Turk J. Bot. 25, 169–175.
Eberhard S., Doubrava N., Marfà V., Mohnen D., Southwick A., Darvill A., Albersheim P., 1989. Pectic cell wall fragments regulate tobacco thin-cell-layer explant morphogenesis. The Plant Cell 1, 747–755.
Florack D.E.A., Dirkse W.G., Visser B., Heidekamp F., Stiekema W.J., 1994. Expression of biologically active hordothionins in tobbaco. Effects of pre- and pro-sequences at the aminoand carboxyl-termini of the hordothionin precursor on mature protein expression and sorting. Plant Mol. Biol. 24, 83–96.
Kumar S., Wittmann C., Heinzle E., 2004. Minibioreactors, Biotechnol. Letters 26, 1–10.
Mungur R., Glass A.D.M., Goodenow D.B., Lighfoot D.A., 2005. Metabolite fingerprinting in transgenic Nicotiana tabacum altered by the Escherichia coli glutamate dehydrogenase gene. J. Biomed. Biotechnol. 2, 198–214.
Murashige T., Skoog F., 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures, Physiol. Plant. 5, 473–497.
Sharp J.M., Doran P.M., 2001a. Characterization of monoclonal antibody fragments produced by plant cells. Biotechnol. Bioeng. 73, 338–346.
Sharp J.M., Doran P.M., 2001b. Strategies for enhancing monoclonal antibody accumulation in plant cell and organ cultures. Biotechnol. Progr. 17, 979–992.
Skrzypek E., Szechyńska-Hebda M., Dąbrowska G., 2007. The influence of phenolic accumulation on callus regeneration abilities of choosen plant species, Zesz. Probl. Post. Nauk Roln. 523, 203–212.
Staub J.M., 2000. High-yield production of human therapeutic protein in tobacco chloroplast. Nature Biotechnol. 18, 333–338.
Szopa J., Kostyń K., 2006. Cell cultures and transgenic plants in biotechnology, Biotechnol. 4(75), 7–17.
Tran Thanh Van K., Toubart P., Cousson A., Darvill A.G., Gollin D.J., Chelf P., Albersheim P., 1985. Manipulation of the morphogenetic pathways of tobacco explants by oligosacharins. Nature 314, 615–617.
Walch-Liu P., Neumann G., Bangerth F., Engels Ch., 2000. Rapid effects of nitrogen form on leaf morphogenesis in tobacco. J. Exper. Bot. 51(343), 227–237.
Walmsley A., Arntzen C., 2000. Plants for delivery of edible vaccines, Curr. Opin. Biotechnol. 11, 126–129.
Wydro M., Kozubek E., Lehman P., 2006. Optimization of transient Agrobacterium-mediated gene expression system in leaves of Nicotiana benthamiana. Acta Bioch. Pol. 33/2, 289–298.
Żur I., Kościelniak J., Dubert F., Płażek A., 2000. Metabolic activity as a possible indicator of morphogenic potential in rape callus cultures – the effect of polyamines, Zesz. Probl. Post. Nauk Roln. 473, 349–357.


Ewa Hanus-Fajerska 
University of Agriculture in Kraków
Krystyna Ciarkowska 
University of Agriculture in Kraków



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