MOLECULAR CHARACETIZATION OF TOMATO YELLOW LEAF CURL VIRUS AND FUSARIUM OXYSPORUM FORMAE SPECIALES AND RACES OF TOMATO AREAS IN NORTHERN CYPRUS


Abstract

Fusarium oxysporum (FO) and viruses have caused wilt, root, crown rots, mosaic, yellowing and curling on tomato plants and have resulted economic yield losses on tomato production areas at Northern Cyprus (NC) in 2011–2015 years. Typical FO symptoms showing greenhouse and open field areas used for collecting 62 plants and suspected Tomato yellow leaf curl virus (TYLCV) of 76 tomato plants have been studied respectively. In the researches, four different primers (uni, sp 13, sp 23, sprl) were used to determine the formae speciales and races of 62 isolates of FO isolates from different locations. PCR analyse studies have revealed that 81% of collected samples were Fusarium oxysporum f.sp. lycopersici (FOL) and 19% of them were Fusarium oxysporum f.sp. radicis-lycopersici (FORL). Additionally, further PCR analyses have identified that 37% of FOL samples were race 1, 15% were race 2 and 29% were race 3 identified respectively. The different samples of 76 tomato plants were tested with specific primers in PCR amplifications. Their results determined that the strains TYLCV-Israel, TYLCV-Sicilia, TYLCV-Mild of TYLCV found. The molecular techniques have suggested that Israel, Sicilia and Mild strain of TYLCV were present in the tomato production areas at NC. The TYLCV races with single or mixed infections and Fusarium oxysporum formae speciales and races are able to identify in molecular techniques in not only accurately but also reliably.


Keywords

tomato; Fusarium oxysporum; Tomato yellow leaf curl virus; PCR; strain

Abak, K., Yılmaz, M.A., Kesici, S. [1991]. Problème de TYLCV en Turquie et moyens utilisés pour diminuer son effet. Proceeding of Seminar on Resistance to TYLCV, Avignon 1991, pp. 28-30.
Abhary, M.K., Anfoka, G.H., Nakhla, M.K., Maxwell, D.P. [2006]. Post transcriptional gene silencing in controlling species of the Tomato yellow leaf curl virus complex. Arch. Virol. 151, 2349–2363.
Accotto, G.P, Navas-Castillo, J., Noris, E., Moriones, E., Louro, D. [2000]. Typing of tomato yellow leaf curl viruses in Europe. Eur. Journal Plant Pathology 106, 179–186.
Anfoka, G.H., Abhary, M., Nakhla, M.K. [2005]. Molecular identification of species of the Tomato yellow leaf curl virus complex in Jordan. Journal Plant Pathology 87, 61–66.
Anfoka, G.M., Abhary, F., Haj Ahmad, A.F., Hussein, A., Rezk, F., Akad, Y.A. [2008]. Survey Of Tomato Yellow Leaf Curl Dısease-Assocıated Vıruses In The Eastern Medıterranean Basın Journal of Plant Pathology 90 [2], 311-320.
Anonim, 2017. KKTC Tarım ve Doğal Kaynaklar Bakanlığı, Tarım Master Planı Taslağı [20 Nisan 2017]. web site: http://www.tdkb.gov.ct.tr
Balmas, V., Scherm, B., Di Primo, P., Rau, D., Marcello, A., Mıgheli, Q. [2005]. Molecular characterisation of vegetative compatibility groups in Fusarium oxysporum f. sp. radicis-lycopersici and f. sp. lycopersici by random amplification of polymorphic DNA and microsatellite-primed PCR. European Journal of Plant Pathology, 111:1-8.
Bel-Kadhi, M.S., Onillon, J.C., Cenis, J.L. [2008]. Molecular characterization of Bemisia tabaci biotypes in Southern Tunisia. Tunisian Journal of Plant Protection 3: 79-85.
Çolak, A., Biçici, M. [2013]. PCR detection of Fusarium oxysporum f.sp. radicis-lycopersici and races of F. oxysporum f.sp. lycopersici of tomato in protected tomato growing areas of Eastern Mediterranean Region of Turkey. Turkısh Journal of Agriculture and Foresty, 37 [4]: 457-467.
Çolak, A., Biçici, M. [2011]. Doğu Akdeniz bölgesi örtü altı domates yetiştiriciliğinde Fusarium oxysporum spesiyal formlarınının simptomatolojik ayrımı ile solgunluk ve kök- kök boğazı çürüklüğü hastalıklarının çıkış, şiddet ve yaygınlıklarının belirlenmesi. Bitki Koruma Bülteni 2011, 51[4]:331-345.
Colak Ates, A., Fidan, A., Karacaoğlu, M., Daşgam, H.Y. [2019]. The identification of the resistance levels of Fusarium oxysporum f. sp. radicis-lycopersici and Tomato yellow leaf curl viruses in different tomato genotypes through traditional and molecular methods. Applied Ecology and Environmental Research 17(2):2203-2218.
Czosnek, H. [2007]. Tomato Yellow Leaf Curl Virus Disease, 85–118.Springer book, p:448.
Czosnek, H. [2008]. Tomato Yellow Leaf Curl Virus. Encyclopedia of Virology, 5 vols. [B.W.J. Mahy and M.H.V. Van Regenmortel, Editors], pp. 138-145 Oxford: Elsevier.
Eppo, [2005]. Tomato Yellow Leaf Curl and Tomato Mottle Begomo Viruses. Oepp/Eppo Bulletin, 35, 271–273. European and Mediterranean Plant Protection Organization. European and Mediterranean Plant Protection Organization [EPPO]. Bulletin OEPP/EPPO Bulletin 35, 271–273.
Fauquet, C.M., Sawyer, S., Idris, A.M., Brown, J.K. [2005]. Sequence analysis and classification of apparent recombinant begomoviruses infecting tomato in the Nile and Mediterranean Basins. Phytopathology 95, 549–555.
Fidan, H., Karacaoğlu, M., Çağlar, B.K., Koç, G., Satar, G. [2011]. Domates Sarı Yaprak Kıvırcıklık Virüsü [Tomato Yellow Leaf Curl Virus [TYLCV]] Irklarının Ve Vektör Bemisia Tabaci [Hemiptera Aleyrodidae] Biotype İlişkisinin Belirlenmesi Türkiye IV. Bitki Koruma Kongresi, 28-30 Haziran 2011.
Ghanim, M., Morin, S., Zeidan, M., Czosnek, H. [1998]. Evidence for Transovarial Transmission of Tomato Yellow Leaf Curl Virus by Its Vector, the Whitefly Bemisia tabaci. Virology 240, 295–303.
Katan, T., Shlevin, E., Katan, J. [1997]. Sporulation of Fusarium oxysporum f. sp. lycopersici on stem surfaces of tomato plants and aerial dissemination of inoculum. Phytopathology 87: 712–719.
Hirano, Y., Arıe, T. [2006]. PCR-based differentiation of Fusarium oxysporum ff. sp. lycopersici and radicis-lycopersici and races of F. oxysporum f. sp. lycopersici. J Gen Plant Pathology 72: 273–283.
Laine, U.L., Pettway, R.E., Katan, T., Kıstler, H.C. [1999]. Population genetic analysis corroborates dispersal of Fusarium oxysporum f. sp. radicis -lycopersici from Florida to Europe. Phtopathology, 89: 623-631.
Moriones, E., Navas-Castillo, J. [2000]. Tomato yellow leaf curl virus, an emerging virus complex causing epidemics worldwide. Virus Res. 71, 123–134.
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Published : 2020-04-14


ATEŞ, A., Fidan, H., & Gökhan, B. (2020). MOLECULAR CHARACETIZATION OF TOMATO YELLOW LEAF CURL VIRUS AND FUSARIUM OXYSPORUM FORMAE SPECIALES AND RACES OF TOMATO AREAS IN NORTHERN CYPRUS. Acta Scientiarum Polonorum Hortorum Cultus, 19(3), 25-35. https://doi.org/10.24326/asphc.2020.3.3

Ayşegül ÇOLAK ATEŞ  aysegulcolak@hotmail.com
Biological Control Research Institute, Kısla Street, 01321 Yuregir, Adana, Turkey  Turkey
Hakan Fidan 
Akdeniz University, Faculty of Agriculture, Plant Protection, Antalya, Turke  Turkey
Bahar Gökhan 
Agriculture Research Institute, Lefkoşa, Northern Cyprus  Turkey




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