MOLECULAR CHARACETIZATION OF TOMATO YELLOW LEAF CURL VIRUS AND FUSARIUM OXYSPORUM FORMAE SPECIALES AND RACES OF TOMATO AREAS IN NORTHERN CYPRUS
Ayşegül Çolak AteşBiological Control Research Institute, Kısla Street, 01321 Yuregir, Adana, Turkey
Hakan FidanAkdeniz University, Faculty of Agriculture, Plant Protection, Antalya, Turke
Bahar GökhanAgriculture Research Institute, Lefkoşa, Northern Cyprus
Fusarium oxysporum (FO) and viruses have caused wilt, root, crown rots, mosaic, yellowing and curling on tomato plants and have resulted economic yield losses on tomato production areas at Northern Cyprus (NC) in 2011–2015 years. Typical FO symptoms showing greenhouse and open field areas used for collecting 62 plants and suspected Tomato yellow leaf curl virus (TYLCV) of 76 tomato plants have been studied respectively. In the researches, four different primers (uni, sp 13, sp 23, sprl) were used to determine the formae speciales and races of 62 isolates of FO isolates from different locations. PCR analyse studies have revealed that 81% of collected samples were Fusarium oxysporum f.sp. lycopersici (FOL) and 19% of them were Fusarium oxysporum f.sp. radicis-lycopersici (FORL). Additionally, further PCR analyses have identified that 37% of FOL samples were race 1, 15% were race 2 and 29% were race 3 identified respectively. The different samples of 76 tomato plants were tested with specific primers in PCR amplifications. Their results determined that the strains TYLCV-Israel, TYLCV-Sicilia, TYLCV-Mild of TYLCV found. The molecular techniques have suggested that Israel, Sicilia and Mild strain of TYLCV were present in the tomato production areas at NC. The TYLCV races with single or mixed infections and Fusarium oxysporum formae speciales and races are able to identify in molecular techniques in not only accurately but also reliably.
Keywords:tomato, Fusarium oxysporum, Tomato yellow leaf curl virus, PCR, strain
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