EFFECT OF MEDIUM TYPE AND PLANT GROWTH REGULATORS ON THE in vitro SHOOT PROLIFERATION OF Cotinus coggygria Scop. ‘ROYAL PURPLE’
Ewelina Jacygrad
Warsaw University of Life Sciences – SGGWAgnieszka Ilczuk
Warsaw University of Life Sciences – SGGWMonika Mikos
Warsaw University of Life Sciences – SGGWKatarzyna Jagiełło-Kubiec
Warsaw University of Life Sciences – SGGWAbstract
The smoke bush (Cotinus coggygria Scop.) is a popular and highly demanded ornamental shrub whose propagation can be problematic. The aim of the study was to test the effect of the mineral salt composition in the medium, concentration of plant growth regulators and the medium pH on the in vitro multiplication and shoot growth of C. coggygria Scop. ‘Royal Purple’. Shoot tips were cultured on the following media: Anderson (AN), Murashige and Skoog (MS) (full- , half- and quarter strength), Nitsch and Nitsch (NN), Quoirin and Lepoivre (QL) or Lloyd and McCown (WPM). Media were supplemented with 6-benzyladenine (BA) in combinations with 1-naphthaleneacetic acid (NAA). The medium type and a concentration of the cytokinin significantly affected the regeneration rate of explants, the number, length and weight of axillary shoots as well as callus growth. The MS medium containing full strength of mineral salts with pH 5.8 was the best. High shoot proliferation (100%) and the highest number of shoots per explant (4.6) were obtained due to the use of 1.0 mg·dm-3 BA in combination with 0.1 mg·dm-3
NAA .
Keywords:
AN, micropropagation, NN, QL, smoke bush, WPMReferences
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Warsaw University of Life Sciences – SGGW
Warsaw University of Life Sciences – SGGW
Warsaw University of Life Sciences – SGGW
Warsaw University of Life Sciences – SGGW
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