Julita Rabiza-Świder

Warsaw University of Life Sciences – SGGW

Ewa Skutnik

Warsaw University of Life Sciences – SGGW

Mari Chodorska

Warsaw University of Life Sciences – SGGW


The quality of cut lily flowers during its postharvest life is important both for growers and customers. It is determined by two factors: longevity of flowers and a general appearance of the entire cut stem during its vase life. The aim of this work was to evaluate the effects of growth regulators (gibberellic acid and benzyladenine) and the preservative (200 mg·dm-3 8HQC+2%S) on quality of cut oriental lily ‘Helvetia,’ a white blooming cultivar very popular on the Polish market. After cutting the flowers were treated for 20 h with growth regulators (GA3 or BA, 500 mg·dm-3) and then placed into distilled water. Non pulsed flowers were kept in a preservative solution composed of 200 mg·dm-3 8HQC and 2% sucrose. Untreated flowers held in distilled water served as a control. During the
senescence of cut lily flowers the soluble protein and free proline contents were determined. As the major problem in the postharvest handling of lilies is leaf yellowing, the effects of postharvest treatments on quality of leaves and their chlorophyll contents were also studied. The preservative increased vase life of cut lily flowers while gibberellic acid improved the quality of leaves. In leaves treated with GA3 the chlorophyll level was 25% higher than in leaves on stems placed into distilled water directly after harvest. The level of soluble protein in petals dropped while free proline accumulated during flower senescence. Flowers treated with GA3 and those placed into 8HQC + 2%S solution showed a delayed protein degradation: the concentrations of soluble proteins after 12 day of vase life was over 3-fold higher than in control flowers. Also the proline accumulation was delayed in flowers on stems placed in the preservative solution, however, no effect of GA3
on the proline level was observed. The soluble protein level correlated with a flower position on the stem being lower in lower flowers as compared to the upper ones.


gibberellic acid, benzyladenine, preservative, vase life, free proline, soluble proteins, chlorophyll

Bates L., Waldren L., Teare J., 1973. Rapid determination of free proline for water – stress studies. Plant and Soil 39, 205–207.
Bradford M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.
Halevy A.H., Mayak S., 1981. Senescence and postharvest physiology of cut flowers. Hort. Rev. 3, 59–143.
Han S., 1997. Preventing postproduction leaf yellowing in Easter lily. J. Amer. Soc. Hort. Sci. 122, 869–872.
Han S.S., 2000. Growth regulators reduce leaf yellowing in Easter lily caused by close spacing and root rot. Hort Sci. 35, 657–660.
Han S., 2003. Role of sugar in the vase solution on postharvest flower and leaf quality of Oriental lily ‘Stargazer’. HortSci. 38(3), 412–416.
Heins R.D., Wallace T.F., Han S.S., 1996. GA4+7 plus benzyladenine reduce leaf chlorosis of greenhouse Easter lilies. HortSci. 31, 597.
Hicklenton P.R., 1991. GA3 and benzylaminopurine delay leaf chlorosis in cut Alstroemeria stems. HortSience 26, 1198–1199.
Inskeep P.W., Bloom R.P., 1985. Extinction coefficients of chlorophyll a and b in N,N-Dimethylformamide and 80% acetone. Plant Physiol. 77(2), 483–485.
Jones R.B., Hill M., 1993. The effect of germicides on the longevity of cut flowers. J. Amer. Soc. Hort. Sci. 118(3), 350–354.
Kapchina V., Yakimova E., Greshkev I., 1991. Is there a relationship between the content of free proline and senescence of cut flowers? Selfregulation of plant metabolism III International symposium. Bułgaria, Warna. 28.09 – 1.10.1991, 192–196.
Karolewski P., 1996. Role of proline in higher plants under conditions of abiotic stress. Wiad. Bot. 40, 67–81.
Moran R., Porath D., 1980. Chlorophyll determination in intact tissue using N,N- Dimethylformamide. Plant Physiol. 65, 478–479.
Nigwekar A.S., Chavan P.D., 1990. The effect of water stress on nitrogen metabolism of horsegram Dolichos biflorus L. Acta Soc. Bot. Pol. 59, 73–80.
Pemberton H.B., Wang Y.T., Mc Donald G.V., 1997. Increase of Easter lily postharvest flower longevity with PBA application to young flower buds. HortScience 32, 458.
Ranwala A.P., Miller W.B., 1998. Gibberellin4+7, benzyloadenine and supplemental light improve postharvest leaf and flower quality of cold- stored ‘Stargazer’ hybrid lilies. J. Amer. Soc. Hort. Sci. 123, 563–568.
Ranwala A.P., Miller W.B., 2002. Effect of gibberellin treatments on flower and leaf quality of cut hybrid lilies. Acta Hort. 570, 205–210.
Rubinstein B., 2000. Regulation of cell death in flower petals. Plant Mol. Biol. 44, 303–318.
Song C., Bang C., Chung S., Kim Y., Lee J., Lee D., 1996. Effects of postharvest pretreatments and preservative solutions on vase life and flower quality of Asiatic hybrid lily. Acta Hort. 414, 277–285.
Strzelecka H., Kamińska J., Kowalski J., Walewska E., 1982. Chemiczne metody badań roślinnych surowców leczniczych. PZWL Warszawa.
van Doorn W.G., Hibma J., de Wit J., 1992. Effect of exogenous hormones on leaf chlorosis in cut flowering branches of Alstroemeria pelegrina. Plant Growth Reg. 11, 59–62.
van Doorn W.G., 2008. Is the onset of senescence in leaf cells of intact plants due to low or high sugar levels? J. Exp. Bot. 59, 1963–1972.
van Doorn W.G., 2011. The postharvest quality of cut lily flowers and potted lily plants. Acta Hort. 900, 255–264.
Wingler A., von Schaewen A., Leegood R.C., Lea P.J., Quick W.P., 1998. Regulation of leaf senescence by cytokinin, sugars and light. Plant Physiol. 116, 329–335.


Julita Rabiza-Świder 
Warsaw University of Life Sciences – SGGW
Ewa Skutnik 
Warsaw University of Life Sciences – SGGW
Mari Chodorska 
Warsaw University of Life Sciences – SGGW



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