EFFECTS OF Trichoderma asperellum [T1] ON Botrytis cinerea [PERS.: FR.], GROWTH AND YIELD OF ORGANIC STRAWBERRY

Jolanta Kowalska

Institute of Plant Protection – National ResearchInstitute


Fungi Trichoderma spp. are present in nearly all soils and other habitats. Most of Trichoderma strains are efficient for control of some pathogens. Some strains induce plant native defence mechanisms. Botrytis cinerea [Pers.: Fr.] causes economic losses on a wide range of cultivated plants and stored fruits. In presented work Trichoderma asperellum [strain T1] isolated from commercial product was selected to apply in the field experiments. The trade bioproduct containing 5 × 108 of Trichoderma conidium per one gram was used in organic field strawberry. Treatments with microorganism-antagonist at concentration 10 g · l-1 were performed as a foliar spraying, three times during the growing season. Applied Trichoderma caused bigger and more vigorous growth. The insignificant presence of B. cinerea on harvested and stored fruits was found. The possibility of use of T. asperellum as biological control agent for post-harvest diseases and for prolongation
of time storage was observed until 7 days after harvest. In the second season of research, statistical significantly higher yield of the treated plants (30.2%) was obtained compare to the untreated plants. Also the weight of aboveground part of plants (24.0%) and number of runners were increased.


Trifender WP, cold keeping of strawberries, foliar treatment, growth and development of plant

Blakeman J.P. ,1993. Pathogens in the foliar environment. Plant Pathology, 42, 479–493.
Braun P.G., Sutton J.C., 1988. Infection cycles and population dynamics of Botrytis cinerea in strawberry leaves. Can. J. Plant Pathol., 10, 133–141
Bristow P.R., Mc Nichol R.J., Wiliamson B., 1986. Infection of strawberry flowers by Botrytis cinerea and its relevance to grey mould development. Ann Appil. Biol., 190, 545–554.
Edwards S.G., Seddon B., 1992. Bacillus brevis as a biocontrol agent against Botrytis cinerea on protected chinese cabbage. 10th International Botrytis Symp: Recent Advances in Botrytis Research (Wageningen, Netherlands), pp. 267–271.
Elad Y., 1995. Mycoparasitism. In: Pathogenesis and host specificity in plant diseases: Histpathological, biochemical, genetic and molecular basis, Vol 2: Eukaryotes. Eds. K. Kohmoto, U.S. Singh and R.S. Singh (Elsevier Science Ltd: Oxford, UK), pp. 289–307.
Elad Y., 1996. Mechanisms involved in the biological control of Botrytis cinerea incited diseases. European Journal of Plant Pathology, 102, 719–732.
Elad Y., Freeman S., 2002. Biological control of fungal plant pathogens. In: Kempken F. (ed.) The Mycota, A comprehensive treatise on fungi as experimental systems for basic and applied research. XI. Agricultural Applications. Springer, Heidelberg, Germany, pp. 93–109.
Freeman S., Minz D., Kolesnik I., Barbul O., Zveibil A., Maymon M., Nitzani Y., Kirshner B., Rav-David D., Bilu A., Dag A, Shafir S., 2004. Trichoderma biocontrol of Colletotrichum acutatum and Botrytis cinerea and survival in strawberry. Europ. J. Plant Pathol., 110, 361–370.
Harman G.E., Howell C.R., Viterbo A., Chet I., Lorito M., 2004. Trichoderma species – opportunistic, avirulent plant symbionts. Nature reviews. Microbiology, 2, (43–56).
Howell C.R., 2003. Mechanisms employed by Trichoderma species in the biological control of plant diseases: The history and evolution of current concepts. Plant Disease, 87, 4–10.
Jacometti M.A., Wratten S.D., Walter M., 2010. Review: Alternatives to synthetic fungicides for Botrytis cinerea management in vineyards. Austral. J. Grape Wine Res., 16, 154–172.
Korolev N., David D.R., Elad Y., 2008. The role of phytohormones in basal resistance and Trichoderma-induced systemic resistance to Botrytis cinerea in Arabidopsis thaliana. BioControl, 53, 667–683.
Kowalska J., 2010. The use of natural substances and microorganisms in organic potatoes production. p: 41–53. In Rembialkowska, E. (Ed.), The impact of organic production methods on the vegetable product quality (pp. 253). Warsaw University of Life Science.
Martinez F., Flores F., Vazllquez-Ortiz E., Lopez-Medina J., 2009. Persistence of Trichoderma asperellum population in strawberry soilless culture growing systems. ISHS Acta Horticulturae, 842: VI International Strawberry Symposium: 223. http://www.actahort.org/book//842/842_223.htm
Mohamed N., Lherminier J., Farmer M.J., Fromentin J., Béno N., Houot V., Milat M.L., Blein J.P., 2007. Defense responses in grapevine leaves against Botrytis cinerea induced by application of a Pythium oligandrum strain or its elicitin, oligandrin, to roots. Phytopathology, 97 (5), 611–620.
Nelson M.E., Powelson M.L., 1988. Biological control of gray mold of snap beans by Trichoderma hamatum. Plant Disease, 72, 727–729.
Peng G., Sutton J.C., 1991. Evaluation of microorganisms for biocontrol of Botrytis cinerea in strawberry. Can. J. Plant Pathol., 13, 247–257.
Porras,M., Barrau C., Romero F., 2009. Influence of Trichoderma and soil solarization on strawberry yield. ISHS Acta Horticulturae, 842: VI International Strawberry Symposium: 75. http://www.actahort.org/book/842/842_75.htm
Sanco /1868/08. 14 May 2008. European Commision Health & Comsumers Directorate-General. Review report for the three active substances Trichoderma gamsii ICC080 (formerly Trichoderma viride strain ICC080), Trichoderma asperellum T11 (formerly identified as Trichoderma viride strain T-25) and Trichoderma asperellum TV1 (formerly identified as Trichoderma viride strain TV1). Finalised in the Standing Committee on the Food Chain and Animal Health at its meeting on 11 July 2008 in view of the inclusion in Annex I of Directive 91/414/EEC.
Sønsteby A., 2002. Antagonism of Trichoderma harzianum (atroviride) P1 and Gliocladium roseum against Botrytis cinerea in organic growing of strawberry. The Nowegian Crop Research Institute. In: NJF-seminar NO346 Organic production of Fruit and Berris, 22. October 2002, 39–43.
Sutton J.C., 1995. Evaluation of microorganisms for biocontrol: Botrytis cinerea and strawberry, a case study. Adv. Plant Pathol., 11, 173–190.
Tronsmo A., Dennis C., 1977. The use of Trichoderma species to control strawberry fruits rots. Netherlands J. Plant Pathogen, 83, 449–455.
Wisniewski M., Biles C., Droby S., McLaughlin R., Wilson C., Chalutz E., 1991. Mode of action of the postharvest biocontrol yeast, Pichia guilliermondii: Characterization of attachment to Botrytis cinerea. Physiol. Molecular Plant Pathol., 39, 245–258.


Jolanta Kowalska 
Institute of Plant Protection – National ResearchInstitute



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