LEAD TOLERANCE MECHANISMS IN Robinia pseudoaccacia L. – AN ATTEMPT TO A PRACTICAL APPROACH
Marzena Wińska-Krysiak
Warsaw University of Life Sciences – SGGWJoanna Bernat
Warsaw University of Life Sciences – SGGWAbstract
Robinia pseudoaccacia plants grown hydroponically were treated Pb(NO3)2 with 15, 45 mg Pb2+ · dm-3. After 6, 12, 24, 72 hours of the metal treatment the plants were collected and dissected organs. The plants accumulated and transported to ground part 0.88% and 1.35% of total accumulated lead for the lower and higher dose of Pb2+ respectively. The level of GSH was differed and depended on organs, dose and time treatment of Pb2+. We investigate (different pattern of expression) expression of RpGSH1 and RpPCS genes in roots. The study showed that glutathione and genes encoded enzymes connected with synthesis of him, plays important role in the process of detoxification in plant.
Keywords:
Robinia pseudoaccacia (L.), lead, glutathione, genes encoded glutathione and phytochelatinsReferences
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Warsaw University of Life Sciences – SGGW
Warsaw University of Life Sciences – SGGW
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