ANTAGONISTIC BACTERIA AND THEIR POST-CULTURE LIQUIDS IN THE PROTECTION OF PEA (Pisum sativum L.) FROM DISEASES
Danuta Pięta
University of Life Sciences in LublinAlina Pastucha
University of Life Sciences in LublinAbstract
The purpose of the paper was to establish the protective effect of the microbiological material prepared from the cells of Bacillus sp. Bk 30 and Pseudomonas sp. Pk
28 and their post-culture liquids against soil-borne pathogenic fungi. The use of biological control improved the emergences, healthiness of pea plants and their yielding. Despite using the microbiological material for seed dressing, pea plants were infected by Altenaria alternata, Ascochyta pisi, Botrytis cinerea, Fusarium culmorum, Fusarium oxysporum f. sp. pisi, Fusarium solani, Phoma exigua, Pythium irregulare, Rhizoctonia solani and Sclerotinia sclerotiorum. Among the applied microbiological material, the best protective effect against plant pathogens was observed for bacterium Bacillus sp. Bk 30 and its postculture liquid.
Keywords:
pea, Bacillus sp., Pseudomonas sp., post-culture liquidsReferences
Abanda-Nipwatt D., Krimm U., Schreiber L., Schwab W., 2006. Dual antagonism of aldehydes and epiphytic bacteria from strawberry leaf surfaces against the pathogenic fungus Botrytis cinerea in vitro. BioControl 51, 3, 279–291.
Babu S., Seetharaman K., Nandakumar R., Johnson I., 2000. Biocontrol efficacy of Pseudomonas fluorescens against Alternaria solani and tomato leaf blight disease. Ann. Plant Prot. Sci., 8, 2, 252–254.
Borecki Z., 1984. Fungicydy stosowane w ochronie roślin. PWN, Warszawa.
Cartwright D. K., Chilton W. S., Benson D. M., 1995. Pyrrolnitrin and phenazine production by Pseudomonas cepacia strain 5.5 B, a biological agent of Rhizoctonia solani. Appl. Microbiol. Biotechnol. 43, 211–121.
Chełkowski J., 1985. Mikotoksyny, wytwarzające je grzyby i mikotoksykozy. Wyd SGGW-AR. Warszawa, 1–96.
De R. K., Dwivedi R. P., Narain U., 2003. Biological control of lentil wilt caused by Fusarium oxysporum f. sp. lentis. Ann. Plant Prot. Sci. 11, 1, 46–52.
Domenech J., Reddy M. S., Kloepper J. W., Ramos B., Gutierrez-Mañero J., 2006.Combined application of the biological produkt LS213 with Bacillus, Pseudomonas or Chryseobacterium from growth promotion and biological control of soil-borne diseases in pepper and tomato. BioControl 51, 2, 245–258.
Goel A. K., Sindhu S. S., Dadarwal K. R., 2000. Pigment diverse mutant of Pseudomonas sp. inhibition of fungal growth and stimulation of growth of Cicer arietinum. Biol. Planet. 43, 4, 563–569.
Hamdan H., Weller D. M., Thomashow L. S., 1991. Relative importance of fluorescent siderophores and other factors in biological control of Gaeumannomyces graminis var. tritici by Pseudomonas fluorescens 2-79 and M4-80R. App. Envir. Microbiol. 57, 3270–3277.
Henry M. B., Lynch J. M., Fermon T. R., 1991. Role of siderophores in the biocontrol of Pseudomonas tolaasii by fluorescens pseudomonad antagonists. J. App. Bacter. 70, 104–108.
Howie W. J., Suslov T. V., 1991. Role of antibiotic biosyntesis in the inhibition of Pythium ultimum in cotton spermosphere and rhizosphere by Pseudomonas fluorescens. Molec. Plant-Microbe Inter. 4, 393–399.
Jain K., WenXi W., BaiGe Z., XiaoCheng S., 1999. Studies on Bacillus subtilis B-903 strain. Inhibitiry action and control effect on plant pathogenic fungi. Chinese J. Biol. Control 15, 4, 157–161.
Lewosz J., 2002. Wykorzystanie mikroorganizmów antagonistycznych wobec patogenów roślin w ochronie roślin. Mat. XLII Sesji Nauk. IOR, Poznań: pp 35.
Manwar A. V., Vaiganker P. D., Bhonge L. S., Chincholkar S. B., 2000. In vitro suppression of plant pathogens by siderophores of fluorescent Pseudomonas. Indian J. Microbiol. 40, 2, 109–112.
Martyniuk S., Masiak D., Stachyra A., Myśków W., 1991. Populacje drobnoustrojów strefy korzeniowej różnych traw i ich antagonizm w stosunku do Gaeumannomyces graminis var. tritici. Pam. Puł. Pr. IUNG 98, 139–144.
Oktaba W., 1987. Metody statystyki matematycznej w doświadczalnictwie. PWN, Warszawa, 488 pp.
Patkowska E., Pastucha A., 2005. Skuteczność płynów pohodowlanych bakterii antagonistycznych w ochronie soi przed grzybami odglebowymi. Acta Agrobotanica, 58, (2), 125–136.
Pięta D., Pastucha A., Patkowska E., 1998. The efficiency of microbiological dressing of soybean seed (Glycine max (L.) Merrill) against root and stem base disease. Ann. Agric. Sci. ser. E, Plant Prot., 95, 1/2, 103–109.
Pięta D., Patkowska E., 2003. The role of antagonistic fungi and bacteria limiting the occurrence of some phytopathogenes inhabiting the soybean soil environment. EJPAU, Horticulture, 6, 2. www.ejpau.media.pl
Pięta D., Patkowska E., Pastucha A., Bełkot M., 2002. Wpływ mikroorganizmów antagonistycznych na ograniczenie porażenia soi przez grzyby chorobotwórcze przeżywające w glebie. Acta Sci. Pol., Hortorum Cultus, 1, 1, 23-30.
Pięta D., 2004. Wybrane zagadnienia z fitopatologii. Wyd. AR, Lublin, 96 pp.
Rasales A. M., Thomashow L., Cook R. J., Mew T. W., 1995. Isolation and identification of antifungal matabolites produced by rice-associated antagonistic Pseudomonas spp. Phytopathology 85, 1028–1032.
Saniewska A., 2000. Antagonistyczny wpływ Bacillus polymyxa na wzrost i rozwój patogenów roślin ozdobnych. Ochr. Rośl. 7, 39–41.
Saniewska A., Orlikowski L. B., Sobiczewski P., 1995. Effectiveness of Bacillus sp. in the control of Phytophthora cryptogea Pethybr. et Laff. M. Mańka (ed.) Environmental biotic factors in integrated plant disease control. Polish Phytopathol. Soc. Poznań, 479–184.
Sobiczewski P., 2002. Biocontrol agent, resistance inducers and genetic engineering for protecton of apple and pear against fire blight (Erwinia amylovora). Book of Abstracts of the 6th Conf. of EFPP "Disease resistance in plant pathology", September 8-14, 2002 Prague, Czech. Republic., 44 pp.
Srivastava R., Shalini R., 2008. Antifungal activiti of Pseudomonas fluorescens against different plant pathogenic fungi. EJEFChe, 7, 4, 2789–2796.
Yeole R. D., Dube H. C., 2000. Siderophore-mediated antibiosis of rhizobacterial fluorescent pseudomonads against certain soil-borne fungal plant pathogens. J. Mycol. Plant Pathol. 30, 3, 335–338.
Babu S., Seetharaman K., Nandakumar R., Johnson I., 2000. Biocontrol efficacy of Pseudomonas fluorescens against Alternaria solani and tomato leaf blight disease. Ann. Plant Prot. Sci., 8, 2, 252–254.
Borecki Z., 1984. Fungicydy stosowane w ochronie roślin. PWN, Warszawa.
Cartwright D. K., Chilton W. S., Benson D. M., 1995. Pyrrolnitrin and phenazine production by Pseudomonas cepacia strain 5.5 B, a biological agent of Rhizoctonia solani. Appl. Microbiol. Biotechnol. 43, 211–121.
Chełkowski J., 1985. Mikotoksyny, wytwarzające je grzyby i mikotoksykozy. Wyd SGGW-AR. Warszawa, 1–96.
De R. K., Dwivedi R. P., Narain U., 2003. Biological control of lentil wilt caused by Fusarium oxysporum f. sp. lentis. Ann. Plant Prot. Sci. 11, 1, 46–52.
Domenech J., Reddy M. S., Kloepper J. W., Ramos B., Gutierrez-Mañero J., 2006.Combined application of the biological produkt LS213 with Bacillus, Pseudomonas or Chryseobacterium from growth promotion and biological control of soil-borne diseases in pepper and tomato. BioControl 51, 2, 245–258.
Goel A. K., Sindhu S. S., Dadarwal K. R., 2000. Pigment diverse mutant of Pseudomonas sp. inhibition of fungal growth and stimulation of growth of Cicer arietinum. Biol. Planet. 43, 4, 563–569.
Hamdan H., Weller D. M., Thomashow L. S., 1991. Relative importance of fluorescent siderophores and other factors in biological control of Gaeumannomyces graminis var. tritici by Pseudomonas fluorescens 2-79 and M4-80R. App. Envir. Microbiol. 57, 3270–3277.
Henry M. B., Lynch J. M., Fermon T. R., 1991. Role of siderophores in the biocontrol of Pseudomonas tolaasii by fluorescens pseudomonad antagonists. J. App. Bacter. 70, 104–108.
Howie W. J., Suslov T. V., 1991. Role of antibiotic biosyntesis in the inhibition of Pythium ultimum in cotton spermosphere and rhizosphere by Pseudomonas fluorescens. Molec. Plant-Microbe Inter. 4, 393–399.
Jain K., WenXi W., BaiGe Z., XiaoCheng S., 1999. Studies on Bacillus subtilis B-903 strain. Inhibitiry action and control effect on plant pathogenic fungi. Chinese J. Biol. Control 15, 4, 157–161.
Lewosz J., 2002. Wykorzystanie mikroorganizmów antagonistycznych wobec patogenów roślin w ochronie roślin. Mat. XLII Sesji Nauk. IOR, Poznań: pp 35.
Manwar A. V., Vaiganker P. D., Bhonge L. S., Chincholkar S. B., 2000. In vitro suppression of plant pathogens by siderophores of fluorescent Pseudomonas. Indian J. Microbiol. 40, 2, 109–112.
Martyniuk S., Masiak D., Stachyra A., Myśków W., 1991. Populacje drobnoustrojów strefy korzeniowej różnych traw i ich antagonizm w stosunku do Gaeumannomyces graminis var. tritici. Pam. Puł. Pr. IUNG 98, 139–144.
Oktaba W., 1987. Metody statystyki matematycznej w doświadczalnictwie. PWN, Warszawa, 488 pp.
Patkowska E., Pastucha A., 2005. Skuteczność płynów pohodowlanych bakterii antagonistycznych w ochronie soi przed grzybami odglebowymi. Acta Agrobotanica, 58, (2), 125–136.
Pięta D., Pastucha A., Patkowska E., 1998. The efficiency of microbiological dressing of soybean seed (Glycine max (L.) Merrill) against root and stem base disease. Ann. Agric. Sci. ser. E, Plant Prot., 95, 1/2, 103–109.
Pięta D., Patkowska E., 2003. The role of antagonistic fungi and bacteria limiting the occurrence of some phytopathogenes inhabiting the soybean soil environment. EJPAU, Horticulture, 6, 2. www.ejpau.media.pl
Pięta D., Patkowska E., Pastucha A., Bełkot M., 2002. Wpływ mikroorganizmów antagonistycznych na ograniczenie porażenia soi przez grzyby chorobotwórcze przeżywające w glebie. Acta Sci. Pol., Hortorum Cultus, 1, 1, 23-30.
Pięta D., 2004. Wybrane zagadnienia z fitopatologii. Wyd. AR, Lublin, 96 pp.
Rasales A. M., Thomashow L., Cook R. J., Mew T. W., 1995. Isolation and identification of antifungal matabolites produced by rice-associated antagonistic Pseudomonas spp. Phytopathology 85, 1028–1032.
Saniewska A., 2000. Antagonistyczny wpływ Bacillus polymyxa na wzrost i rozwój patogenów roślin ozdobnych. Ochr. Rośl. 7, 39–41.
Saniewska A., Orlikowski L. B., Sobiczewski P., 1995. Effectiveness of Bacillus sp. in the control of Phytophthora cryptogea Pethybr. et Laff. M. Mańka (ed.) Environmental biotic factors in integrated plant disease control. Polish Phytopathol. Soc. Poznań, 479–184.
Sobiczewski P., 2002. Biocontrol agent, resistance inducers and genetic engineering for protecton of apple and pear against fire blight (Erwinia amylovora). Book of Abstracts of the 6th Conf. of EFPP "Disease resistance in plant pathology", September 8-14, 2002 Prague, Czech. Republic., 44 pp.
Srivastava R., Shalini R., 2008. Antifungal activiti of Pseudomonas fluorescens against different plant pathogenic fungi. EJEFChe, 7, 4, 2789–2796.
Yeole R. D., Dube H. C., 2000. Siderophore-mediated antibiosis of rhizobacterial fluorescent pseudomonads against certain soil-borne fungal plant pathogens. J. Mycol. Plant Pathol. 30, 3, 335–338.
Danuta Pięta
University of Life Sciences in Lublin
University of Life Sciences in Lublin
Alina Pastucha
University of Life Sciences in Lublin
University of Life Sciences in Lublin
License
Articles are made available under the conditions CC BY 4.0 (until 2020 under the conditions CC BY-NC-ND 4.0).
Submission of the paper implies that it has not been published previously, that it is not under consideration for publication elsewhere.
The author signs a statement of the originality of the work, the contribution of individuals, and source of funding.
