A COMPARATIVE ANALYSIS OF PLANT GROWTH-PROMOTING TRAITS OF Pseudomonas AND Bacillus STRAINS ISOLATED FROM Lolium perenne RHIZOSPHERIC SOIL IN VOJVODINA (SERBIA) AND THEIR EFFECT ON THE PLANT YIELD


Abstract

The objective of this work was to do a comparative study of Pseudomonas and Bacillus isolates for their plant growth-promoting (PGP) potential, monitoring the impact of selected isolates on the yield of English ryegrass (Lolium perenne). Isolation, physiological and biochemical characterization, in vitro assay of enzymatic and plant-growth promoting activities of isolates were done. Pseudomonas isolates have been shown to have the ability to use different sources of carbon, to live in the condition of low pH as well as temperature and to produce siderophore. On the other hand, Bacillus isolates have the ability to solubilize phosphate, to produce a greater amount of indol-3-acetic acid (IAA) than Pseudomonas isolates and have an inhibitory effect on the growth of phytopathogenic fungi. In other investigated traits, isolates were similar. The use of Pseudomonas sp. P12 and Bacillus sp. B1 isolates had a positive effect on the plant mass and total yield, which indicate that the use of these isolates can result in a better yield of forage crops.


Keywords

antifungal activity; auxin; enzymatic activities; siderophores; grass

Ahmad, P., Kumar, A., Gupta, A., Hu, X., Hakeem, K.R., Azooz, M.M., Sharma, S. (2012). Polyamines: Role in Plants under Abiotic Stress. In: Crop Production for Agricultural Improvement, Ashraf, M., Öztürk, M., Ahmad, M.S.A., Aksoyet A. (eds.), chap. 19, 491–512. DOI: 10.1007/978-94-007-4116
Alina, S.O., Constantiscu, F., Calina Petruta, C. (2015). Biodiversity of Bacillus subtilis group and beneficial traits of Bacillus species useful in plant protection. Rom. Biotechnol. Lett., 20(5), 10737−10750.
Aneja, K.R. (2003). Experiments in microbiology plant pathology and biotechnology, 4th ed. New Delhi, India.
Balasubramanian, N., Simões, N. (2014). Bacillus pumilus S124A carboxymethylcellulase; a thermo stable enzyme with a wide substrate spectrum utility. Int. J. Biol. Macromol., 67, 132–139. DOI: 10.1016/j.ijbiomac.2014.03.014
Beneduzi, A., Ambrosini, A., Passaglia, L.M.P. (2012). Plant growth-promoting rhizobacteria (PGPR): Their potential as antagonists and biocontrol agents. Genet. Mol. Biol., 35(4), 1044−1051. DOI: 10.1590/s1415-47572012000600020
Bianco, C., Imperlini, E., Defez, R. (2009). Legumes like more IAA. Plant Signal. Behav., 4, 763–765. DOI: 10.4161/psb.4.8.9166
Datta, M., Palit, R., Sengupta, C., Pandit, M.K., Banerjee, S. (2011). Plant growth promoting rhizobacteria enhance growth and yield of chili (Capsicum annuum L.) under field conditions. Aust. J. Crop Sci., 5(5), 531–536.
Davies, P.J. (2010). Plant Hormones: their nature, occurrence, and functions. Plant Horm., 1–5. DOI: 10-1007/978-1-4020-2686
Etesami, H., Alikhani, H.A., Hosseini, H.M. (2015). Indole-3-acetic acid (IAA) production trait, a useful screening to select endophytic and rhizosphere competent bacteria for rice growth promoting agents. MethodsX, 2, 72–78. DOI: 10.1016/j.mex.2015.02.008
Frey-Klett, P., Chavatte, M., Clausse, M.L., Courrier, S., Le Roux, C., Raaijmakers, J., Martinotti, M.G., Pierrat, J.C., Garbaye, J. (2005). Ectomycorrhizal symbiosis affects functional diversity of rhizosphere fluorescent pseudomonads. New Phytol., 165, 317–328. DOI: 10.1111/j.1469-8137
Garcia, J.A.L., Probanza, A., Ramos, B., Palomino, M.R., Manero, F.J.G. (2004). Effect of inoculation of Bacillus lichenoformis on tomato and pepper. Agronomie, 24(4), 169−176. DOI: 10.1051/agro: 2004020
Hassanein, W.A., Awny, N.M., El-Mougith, A.A., Salah, El-Dien, S.H. (2009). The antagonistic activities of some metabolites produced by Pseudomonas aeruginosa Sha8. J. Appl. Sci. Res., 5(4), 404−414.
Karagoz, K., Ates, F., Kotan, R., Cakmakci, K. (2012). Characterization of plant growth promoting traits of bacteria isolated from the rhizosphere of grapevine grown in alkaline and acidic soils. Eur. J. Soil Biol., 50, 144−150. DOI: 10.1016/ejsobi.2012.01.007
Khan, K.S., Joergensen, R.G. (2009). Changes in microbial biomass and P fractions in biogenic household waste compost amended with inorganic P fertilizers. Bioresour. Technol., 100, 303−309. DOI: 10.1016/j.biotech.2008.06.002
Kim, H., Sang, M.K., Myung, I., Chun, S., Kim, K.D. (2009). Characterization of Bacillus luciferensis strain KJ2C12 from pepper root, a biocontrol agent of Phytophthora Blight of pepper. J. Plant Pathol., 25(1), 62−69. DOI: 10.5423/PPJ.2009.25.1.062
Mehnaz, S., Weselovski, B., Mufti, F.A., Zahid, S., Lazarovits, G., Igbal, J. (2009). Izolation, characterization and effect of fluorescent pseudomonads on micropropagated sugarcane. Can. J. Microbiol., 55, 1007−1011. DOI: 10.1139/w09-050
Mishra, J., Arora, N.K. (2018). Secondary metabolites of fluorescent pseudomonads in biocontrol of phytopathogens for sustainable agriculture. Appl. Soil. Ecol., 125, 35−45. DOI: 10.1016/j.apsoil.2017.12.004
Pikovskaya, R.I. (1948). Mobilization of phosphorous in soil in connection with vital activity of some microbial species. Microbiol., 17, 362−370.
Ramesh, R., Joshi, A.A., Ghanekar, M.P. (2009). Pseudomonads: major antagonistic endophytic bacteria to suppress bacterial wilt pathogen, Ralstonia solanacearum in the eggplant (Solanum melongena L.). World J. Microbiol. Biotechnol., 25, 47−55. DOI: 10.1007/s11274.008.9859.3
Rokhzadi, A., Asgharzadeh, A., Darvish, F., Nour-Mohammadi, G., Majidi, E. (2008). Influence of plant growth promoting rhizobacteria on dry matter accumulation of Chickpea (Cicer arietinum L.) under field conditions. Res. J. Agr. Env. Sci., 3(2), 253−257.
Schwyn, B., Neilands, J.B. (1987). Universal chemical assay for the detection and determination of siderophores. Anal. Biochem., 160, 47−56. DOI: 10.1016/003.2697 (87)90612-9
Shameer, S., Prasad, T.N.V.K.V. (2018). Plant growth promoting rhizobacteria for sustainable agricultural practices with special reference to biotic and abiotic stresses. Plant Growth Regul., 84, 603−615. DOI: 10.1007/s10725-017-0365-1
Spiers, A.J., Buckling A., Rainey P.B. (2000). The causes of Pseudomonas diversity. Microbiol., 146, 2345−2350. DOI: 10.1099/00221287.146.10.2345
Stamenov, D., Jarak, M. (2012). The Effect of Microbial Inoculants on the Yield of English Ryegrass, Number and Diversity of Rhizosperic Microorganisms. Conference proceedings. International Conference on BioScience: Biotechnology and Biodiversity – Step in the Future – The Forth Joint UNS – PSU Conference, Novi Sad, Serbia, June 18–20, 401−415.
Stamenov, D., Jarak, M., Đurić, S., Hajnal-Jafari, T. (2012). The use of plant growth promoting rhizobacteria in the production of English ryegrass. Plant Soil Environ., 58(10), 477−480. DOI: 10.17221/132/2012.PSE
Supanjani Han, H.S., Jung, J.S., Lee, K.D. (2006). Rock phosphate-potassium and rock-solubilising bacteria as alternative, sustainable fertilisers. Agron. Sustain. Dev., 26(4), 233−240. DOI: 10.1051/agro: 2006020
Suresh, A., Pallavi, P., Srinivas, P., Kumar, V.P., Chandra, S.J. (2010). Plant growth promoting activities of fluorescent pseudomonads associated with some crop plants. Afr. J. Microbiol. Res., 4, 1491−1494.
Toure, Y., Ongena, M., Jacques, P., Guiro, A., Thonart, P. (2004). Role of lipopeptides produced by Bacillus subtilis GA1 in the reduction of grey mould disease caused by Botrytis cinerea on apple. J. Appl. Microbiol., 96, 1151−1160. DOI: 10.1111/j.1365.2672.
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Published : 2020-04-15


Stamenov, D., Hajnal-Jafari, T., Najvirt, B., Anđelković, S., Tomić, J., & Đurić, S. (2020). A COMPARATIVE ANALYSIS OF PLANT GROWTH-PROMOTING TRAITS OF Pseudomonas AND Bacillus STRAINS ISOLATED FROM Lolium perenne RHIZOSPHERIC SOIL IN VOJVODINA (SERBIA) AND THEIR EFFECT ON THE PLANT YIELD. Acta Scientiarum Polonorum Hortorum Cultus, 19(3), 37-45. https://doi.org/10.24326/asphc.2020.3.4

Dragana Stamenov  dragana.stamenov@polj.uns.ac.rs
University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia  Serbia
Timea I. Hajnal-Jafari 
University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia  Serbia
Biljana Najvirt 
University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia  Serbia
Snežana Anđelković 
Institute of Forage Crops, Kruševac, Serbia  Serbia
Jelena Tomić 
Fruit Research Institute, Department for Technology of Fruit Growing, Čačak, Serbia  Serbia
Simonida Đurić 
University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia  Serbia




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