THE EFFECT OF GROWTH REGULATORS AND CULTURE CONDITIONS ON THE CALLUS INDUCTION IN TOMATO Lycopersicon Sp.
Danuta Rzepka-Plevneš
Agricultural University in SzczecinDanuta Kulpa
Agricultural University in SzczecinMarta Grabiec
Agricultural University in SzczecinKatarzyna Kowalczys
Research Institute of Pomology and Floriculture, SkierniewiceJadwiga Kurek
Agricultural University in SzczecinAbstract
Effect of auxins: (NAA, IAA), cytokinin BAP and culture conditions (light, darkness) on callus induction in cotyledons of tomato cultivar ‘Maskotka’ and the wild form of Lycopersicon peruvianum was investigated. Callus was obtained in all experimental combinations, except of the culture with L. peruvianum on the medium with 1.0 mg×dm-3 of IAA. Callus weight, colour and structure depended on the tomato genotypes
and experimental conditions. Best medium for the culture of tomato cultivar ‘Maskotka’ and the wild form L. peruvianum proved to be MS medium supplemented with 2.0 mg×dm-3 of IAA and 1.0mg×dm-3 of BAP.
Keywords:
tomato, cotyledons, callus, growth regulators, light, darknessReferences
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Agricultural University in Szczecin
Agricultural University in Szczecin
Agricultural University in Szczecin
Research Institute of Pomology and Floriculture, Skierniewice
Agricultural University in Szczecin
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