THE USE OF NANO-SILVER FOR DISINFECTION OF Pennisetum alopecuroides PLANT MATERIAL FOR TISSUE CULTURE
Marzena ParzymiesDepartment of Ornamental Plants, Dendrology and Landscape Architecture, University of Life Sciences in Lublin, Poland
Krystyna PudelskaDepartment of Ornamental Plants, Dendrology and Landscape Architecture, University of Life Sciences in Lublin, Poland
Monika PoniewozikDepartment of Ornamental Plants, Dendrology and Landscape Architecture, University of Life Sciences in Lublin, Poland
Initiation of tissue culture of many plant species is a very difficult stage due to appearance of many contaminations. The other problem might be a choice of media for regeneration. Initiation of grass species tissue cultures are thought to be very difficult. Therefore, a research was undertaken to evaluate the use of nano-silver particles for plant material disinfection and to estimate a medium Pennisetum alopecuroides. The plant material were buds and nodal explants that were disinfected in 2% NaOCl for 30 min or 0.1% HgCl2 for 1 min. Half of the explants disinfected with NaOCl were soaked in 50, 100 or 250 mg·dm Ag–3 NPs for 1 hour. Explants not soaked in nano-silver were placed on media with Ag NPs at concentrations of 4, 8 or 16 mg·dm–3. An influence of growth regulators on Pennisetum alopecuroides was evaluated in vitro. Regenerated shoots were placed on MS media with: 3 mg·dm–3 BA + 0.3 mg·dm–3 IBA, 3 mg·dm–3 KIN + 0.3 mg·dm–3 IAA, 1 mg·dm–3 BA + 0.1 mg·dm–3 IBA. It was observed that the use of nano-silver particles lowered the level of contamination. The best results were obtained when Ag NPs was used at concentration of 100–250 mg·dm–3 alone or as a supplementation of the media, at concentration of 4 mg·dm–3 for nodes and 16 mg·dm–3 for adventitious buds. The use of nodal explants allowed to obtain less contamination. Regeneration depended on a media content. The most regenerated shoots were obtained on the MS media supplemented with 1 mg·dm–3 BA and 0.1 mg·dm–3 IBA.
Keywords:nano-particles, culture initiation, micropropagation, plant growth regulators, contaminations, regeneration
Abdi, G., Salehi, H., Khosh-Khui, M. (2008). Evaluation the potential of nano silver for removal of bacterial contaminants in valerian (Valeriana officinalis L.) tissue culture. J. Biol. Environ. Sci., 30(5), 709–714.
Alexandrova, K.S., Denchev, P.D., Conger, B.V. (1996). Micropropagation of switchgrass by node culture. Crop Sci., 36(6), 1709–1711.
Arab, M.M., Yadollahi, A., Hosseini-Mazinani, M., Bagheri, S. (2014). Effects of antimicrobial activity of silver nanoparticles on in vitro establishment of G × N15 (hybrid of almond × peach) rootstock. J. Genets Engin. Biotechnol., 12(2), 103–110.
Arockiasamy, S., Sahaya Rani, S., Ignacimuthu, S., Melchias, G. (2006). Efficient protocols for in vitro regeneration Pennisetum glaucum (L.). Br. Indian J. Exp. Biol., 44, 757–761.
Asli, S., Neumann, P.M. (2009). Colloidal suspensions of clay or titanium dioxide nanoparticles can inhibit leaf growth. Plant Cell Environ., 32(5), 577–584.
Balilashaki, K., Naderi, R., Kalantari, S., Soorni, A. (2014). Micropropagation of Phalaenopsis amabilis cv. ‘Cool Breeze’ with using of flower stalk nodes and leaves of sterile obtained from node cultures. Int. J. Farm. Alli. Sci., 3(7), 823–829.
Bhatt, I., Tripathi, B.N. (2011). Interaction of engineered nanoparticles with various components of the environment and possible strategies for their risk assessment. Chemosphere, 82, 308–317.
Bliss, C.I. (1938). The transformation of percentages for use in the analysis of variance. Ohio J. Sci., 38(1), 9–12.
Dar, Ch.T., Abdullah, J.O., Namasivayam, P., Roowi, S.H. (2012). Sterilization of Hibiscus rosa – sinensis L. vegetative explants sourced from plants grow in open environment and influences of organic ingredients on in vitro direct regeneration. Am. J. Plant Sci., 3, 791–798.
Dibrov, P., Dzioba, J., Gosink, K.K., Häse, C.C. (2002). Chemiosmotic Mechanism of Antimicrobial Activity of Ag+ in Vibrio cholera. Antimicrob. Agents Chemother., 46(8), 2668–2670. DOI: 10.1128/AAC.46.8.2668–2670
Fakhrfeshani, M., Bagheri, A., Sharifi, A. (2012). Disinfecting effects of nano silver fluids in gerbera (Gerbera jamesonii) capitulum tissue culture. J. Biol. Environ. Sci., 6(17), 121–127.
Jha, P., Yadav, C.B., Anjaiah, V., Bhat, V. (2009). In vitro plant regeneration through somatic embeyogenesis and direct shoot organogenesis in Pennisetum glaucum (L.) R. Br. In Vitro Cell. Dev. Biol.-Plant., 45, 145–154.
Jia, W., Du, X., Liu, H. You, H., Mu, J. (2011). Estabilishment of plantlet regeneration system of tree peony through lateral buds engraving. International Conference on Remote Sensing, Environmental and Transportation Engineering, 24–26 June 2011, 7569–7572.
Jo, D., Lee, T., Kim, J. (2011). Nanotechnology and nanotoxicology in retiopathy. Int. J. Mol. Sci., 12(11), 288–301.
Kharrazi M., Nemati H., Tehranifar A., Bagheri A., Sharifi A. (2011). In vitro culture of carnation (Dianthus caryophyllus L.) focusing on the problem of vitrification. J. Biol. Environ. Sci., 13, 1–6.
Klaine, S.J., Alvarez, P.J.J., Batley, G.E., Fernandes, T.F., Handy, R.D., Lyon, D.Y., Mahendra, S., Mclaughlin, M.J. (2008). Nanomaterials in the environment: bahavior, fate, bioavailabililty, and effects. Environ. Toxicol. Chem., 27, 1825–1851.
Kumari, M., Mukherjee, A., Chandrasekaran, N. (2009). Genotoxicity of silver nanoparticles in Allium cepa. Sci. Total Environ., 407, 5243–5246.
Larue C., Castillo-Michel H., Sobanska S., Cécillon L., Bureau S., Barthès V., Ouerdane L., Carrière M., Sarret H. (2014). Foliar exposure of the crop Lactuca sativa to silver nanoparticles: Evidence for internalization and changes in Ag speciation. J. Hazard. Mater., 264, 98–106.
Lee, C.W., Mahendra, S., Zodrow, K., Li, D., Tsai, Y.C., Braam, J., Alvarez, P.J. (2010). Developmental phytotoxicity of metal oxide nanoparticles to Arabidopsis thaliana. Environ. Toxic Chem., 29(3), 669–675.
McGrath, S.P., Zhao, E.J. (2003). Phytoextraction of metals and metalloids from contaminated soils. Curr. Opinion Biotech., 14, 277–282.
Mekonnen, T., Diro, M., Sharma, M. (2013). An alternative safer and cost effective surface sterilization method for sugarance (Saccharum officinarum L.) explants. Afr. J. Biol., 1(1), 29–32.
Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant, 15, 473–497.
Nabeel, K.A.A. (2011). Using silver nano-particles to increase efficiency of sterile solution for in vitro techniques. Iraqi J. Canc. Med. Genet., 4, 48–51.
Oldach, K.H., Morgenstern, A., Rother, S., Girgi, M. (2001). Efficient in vitro plant regeneration from immature zygotic embryos of pearl millet (Pennisetum glaucum (L.) R.Br.) and Shorgum bicolor (L.) Moench. Plant Cell. Rep., 20, 416–421.
Oyebanji, O.B., Nweke, O., Odebunmi, O., Galadima, N.B., Idris, M.S., Nnodi, U.N., Afolabi, A.S., Ogbadu, G.H. (2009). Simple, effective and economical explant – surface sterilization protocol for covepa, rice and sorghum seeds. Afr. J. Biotech., 8(20), 5395–5399.
Pepo, P., Toth, S. (2005). The role nitrogen and phosphorus source in Miscanthus in vitro cultures. Cereal Res. Commun. 33(2/3), 549–552, https://doi.org/10.1556/CRC.33.2005.2-3.118
Rostami, A.A., Shahsavar, A.R. (2012). In vitro micropropagation of olive (Olea europaea L.) ‘Mission’ by nodal segments. J. Biol. Enciron. Sci., 17, 155–159.
Sarmast, M.K., Salehi, H., Khosh-Khui, M. (2011). Nano silver treatment is effective in reducing bacterial contaminations of Araucaria excelsa R. Br. var. glauca explants. Acta Biol. Hung., 62(4), 477–484.
Shokri, S., Babaei, A.R., Ahmadian, M., Hessami, S., Arab, M.M. (2014). The effects of different concentrations of nano-silver on elimination of bacterial contaminations and phenolic exudation of rose (Rosa hybrida L.) in vitro culture. Int. J. Farm. Alli. Sci., 3(1), 50–54.
Singh, P., Patel, R.M., Modi, P.K. (2014). Effect of sterilization treatments on contamination of pomegranate cv. Ganesh explants. Bioinfolet, 11(4a), 1087–1089.
Taghizadeh, M., Solgi, M. (2014). The application of essential oils and silver nanoparticles for sterilization of Bermuda grass explants in vitro culture. Int. J. Hort. Sci. Technol., 1(2), 131–140.
Tymoszuk A., 2014. Application of silver and copper nanocolloids in disinfection of explants in chrysanthemum in vitro cultures. Book of Abstracts. NanoPL 2014. “Nanotechnology and Advanced Materials for Innovative Industry”, Inno-Tech Expo, The 2nd International Expo of Innovativeness and New Technologies, 16–17 October 2014, Kielce, Poland, 47–48.
Yildiz, M., Er, C. 2002. The effect of sodium hypochlorite solutions on in vitro seedling growth and shoot regeneration of flax (Linnum usitatissimum). Naturwissenschaften, 89, 259–261.
Zhu, H., Han, J., Xiao, J.Q., Jin, Y., 2008. Uptake translocation and accumulation of manufactured iron oxide nanoparticles by pumpkin plant. J. Environ. Monit., 10, 713–717.
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