THE USE OF NANO-SILVER FOR DISINFECTION OF Pennisetum alopecuroides PLANT MATERIAL FOR TISSUE CULTURE
Marzena Parzymies
Department of Ornamental Plants, Dendrology and Landscape Architecture, University of Life Sciences in Lublin, PolandKrystyna Pudelska
Department of Ornamental Plants, Dendrology and Landscape Architecture, University of Life Sciences in Lublin, PolandMonika Poniewozik
Department of Ornamental Plants, Dendrology and Landscape Architecture, University of Life Sciences in Lublin, PolandAbstract
Initiation of tissue culture of many plant species is a very difficult stage due to appearance of many contaminations. The other problem might be a choice of media for regeneration. Initiation of grass species tissue cultures are thought to be very difficult. Therefore, a research was undertaken to evaluate the use of nano-silver particles for plant material disinfection and to estimate a medium Pennisetum alopecuroides. The plant material were buds and nodal explants that were disinfected in 2% NaOCl for 30 min or 0.1% HgCl2 for 1 min. Half of the explants disinfected with NaOCl were soaked in 50, 100 or 250 mg·dm Ag–3 NPs for 1 hour. Explants not soaked in nano-silver were placed on media with Ag NPs at concentrations of 4, 8 or 16 mg·dm–3. An influence of growth regulators on Pennisetum alopecuroides was evaluated in vitro. Regenerated shoots were placed on MS media with: 3 mg·dm–3 BA + 0.3 mg·dm–3 IBA, 3 mg·dm–3 KIN + 0.3 mg·dm–3 IAA, 1 mg·dm–3 BA + 0.1 mg·dm–3 IBA. It was observed that the use of nano-silver particles lowered the level of contamination. The best results were obtained when Ag NPs was used at concentration of 100–250 mg·dm–3 alone or as a supplementation of the media, at concentration of 4 mg·dm–3 for nodes and 16 mg·dm–3 for adventitious buds. The use of nodal explants allowed to obtain less contamination. Regeneration depended on a media content. The most regenerated shoots were obtained on the MS media supplemented with 1 mg·dm–3 BA and 0.1 mg·dm–3 IBA.
Keywords:
nano-particles, culture initiation, micropropagation, plant growth regulators, contaminations, regenerationReferences
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Department of Ornamental Plants, Dendrology and Landscape Architecture, University of Life Sciences in Lublin, Poland
Department of Ornamental Plants, Dendrology and Landscape Architecture, University of Life Sciences in Lublin, Poland
Department of Ornamental Plants, Dendrology and Landscape Architecture, University of Life Sciences in Lublin, Poland
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