CHINESE CABBAGE BrMYB34.2 TRANSCRIPTION FACTOR REGULATES INDOLIC GLUCOSINOLATES BIOSYNTHESIS IN Arabidopsis
Ye Zhao
College of Forestry, Shandong Agricultural University, Taian, Shandong 271018, ChinaYongqiang Zhang
College of Forestry, Shandong Agricultural University, Taian, Shandong 271018, ChinaXianfeng Guo
College of Forestry, Shandong Agricultural University, Taian, Shandong 271018, ChinaYan Ma
College of Forestry, Shandong Agricultural University, Taian, Shandong 271018, ChinaPeng Zhang
Mountain Tai Scenic Area Management Committee, Taian, Shandong 271018, ChinaHongling Liu
Mountain Tai Scenic Area Management Committee, Taian, Shandong 271018, ChinaGang Liu
Mountain Tai Scenic Area Management Committee, Taian, Shandong 271018, ChinaJing Guo
College of Forestry, Shandong Agricultural University, Taian, Shandong 271018, ChinaAbstrakt
Glucosinolates (GS) are a group of sulfur- and nitrogen-rich plant secondary metabolites that originate from amino acids and exist mainly in plants in the order Brassicales, such as Arabidopsis thaliana (Arabidopsis) and Chinese cabbage (Brassica rapa ssp. pekinensis). To date, several regulatory components responsible for GS biosynthesis have been identified in Arabidopsis. However, the functions of GS biosynthesis regulators in Chinese cabbage have not been clarified. In our current study, a putative ATR1/MYB34 orthologous gene, BrMYB34.2, was isolated from Chinese cabbage leaves. To investigate the function of this gene, we engineered Arabidopsis plants that overexpress BrMYB34.2 ectopically and phenotypic analysis was performed. Moreover, we assayed the accumulation levels of indolic GS (IGS) and aliphatic glucosinolates in transgenic plants and test the expression of key genes of IGS biosynthesis and tryptophan synthesis by Real-time quantitative PCR. And further analysed the resistance of transgenic plants in 5MT stress treatment. The results indicate that ectopic expression of the BrMYB34.2 gene in Arabidopsis was able to up-regulate the accumulation level of IGS due to the increased expression of IGS and Trp biosynthetic genes. Moreover, overexpression of BrMYB34.2 conferred Arabidopsis 5MT resistance. These results suggest that the BrMYB34.2 gene may function as one of the regulators of IGS and Trp biosynthesis in Chinese cabbage.
Słowa kluczowe:
BrMYB34.2, indolic glucosinolate, tryptophan, Chinese cabbageBibliografia
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College of Forestry, Shandong Agricultural University, Taian, Shandong 271018, China
College of Forestry, Shandong Agricultural University, Taian, Shandong 271018, China
College of Forestry, Shandong Agricultural University, Taian, Shandong 271018, China
College of Forestry, Shandong Agricultural University, Taian, Shandong 271018, China
Mountain Tai Scenic Area Management Committee, Taian, Shandong 271018, China
Mountain Tai Scenic Area Management Committee, Taian, Shandong 271018, China
Mountain Tai Scenic Area Management Committee, Taian, Shandong 271018, China
College of Forestry, Shandong Agricultural University, Taian, Shandong 271018, China
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