THE EFFECT OF INFESTATION WITH ISOLATES OF Trichoderma sp. ON MYCELIUM GROWTH AND YIELDING IN SINGLE-SPORE HETEROKARYOTIC CULTURES OF Agaricus bisporus (Lange) Imbach
Krzysztof Sobieralski
Poznań University of Life SciencesMarek Siwulski
Poznań University of Life SciencesLidia Błaszczyk
Institute of Plant Genetics, Polish Academy of Science, PoznańDorota Frużyńska-Jóźwiak
Poznań University of Life SciencesJolanta Lisiecka
Poznań University of Life SciencesAbstrakt
The occurrence of fungi from the genus Trichoderma constitutes a serious problem in the culture of Agaricus bisporus leading to considerable losses. The aim of the
investigations was to compare mutual interaction between some isolates of Trichoderma sp. and single-spore heterokaryotic cultures of A. bisporus on agar media and in cultivation conditions. The first stage of the study comprised assessment of growth for isolates of T. harzianum, T. agrressivum, T. atroviride and T. hamatum on PDA medium and growth of four heterokaryotic single-spore and strain Amycel 2600 A. bisporus cultures on peatmanure substrate. Moreover, the individual biotic effect (IEB) between isolates of Trichoderma and the tested A. bisporus cultures was also analysed. The second stage consisted in the evaluation of the volume of A. bisporus yield on the substrate, to which mycelia isolates of Trichoderma sp. were introduced. It was found that the analysed cultures and the cultivar strain of A. bisporus responded differently to the presence of Trichoderma sp. isolates both in terms of mycelium growth and the yield volume.
Słowa kluczowe:
green mould, button mushroom, biotic effect, yieldBibliografia
Anderson M.G., Beyer D.M., Wuest P.J., 2001. Yield Comparison of Hybrid Agaricus Mushroom Strains as a Measure of Resistance to Trichoderma Green Mold. Plant Disease 85(7), 731–734.
Błaszczyk L., Popiel D., Chełkowski J., Koczyk G., Samuels G.J., Sobieralski K., Siwulski M., 2011. Species diversity of Trichoderma in Poland. J. Appl. Genetics 52, 233–243.
Błażej J., Tekiela A., 2002. Występowanie grzybów pasożytniczych i konkurencyjnych dla pieczarki (Agaricus bisporus (Lange) Sing) w różnych podłożach i okrywie. Acta Sci. Pol. Hortorum Cultus 1(2), 33–41. Romaine C.P
Chen X., Ospina-Giraldo M.D., Wilkinson V., Royse D.J.,., 2003. Resistance of Pre- and Post-epidemic Strains of Agaricus bisporus to Trichoderma aggressivum f. aggressivum. Plant Disease 87, 1457–1461.
Fritsche G., 1972. Beispiel der Wirkung der Einsporkulturauslese als zuchterische Methode beim Kulturchampignon. Theor. Appl. Genet. 42, 62–64.
Frużyńska-Jóźwiak D., Siwulski M., Sobieralski K., Sas-Golak I., Błaszczyk L., 2011. Impact of Trichoderma isolates on the mycelium development of wild strains of Coprinus comatus (Müll.) S.F. Gray. J. Plant Protection Res. 51(2), 162–165.
Hatvani L., Antal Z., Manczinger L., Szekeres A., Druzhinina I.S., Kubicek C.P., Nagy A., Nagy E., Vágvölgyi C., Kredics L., 2007. Green mold diseases of Agaricus and Pleurotus spp. are caused by related but phylogenetically different Trichoderma species. Phytopathology 97, 532–537.
Hermosa M.R., Grondona I., Iturriaga E.A., Diaz-Minguez J.M., Castro C., Monte E., Garcia-Acha I., 2000. Molecular characterization and identification of biocontrol isolates of Trichoderma. Appl. Environ. Microbiol. 66, 1890–1898.
Kneebone L.R., Patton T.G., Schultz P.G., 1976. Improvement of the brown variety of Agaricus bisporusby single spore selection. Mushroom Sci. 9, 237–243.
Kredics L., Garcia Jimenez L., Naeimi S., Czifra D., Urban P., Manczinger L., Vágvölgyi C., Hatvani L., 2010. A challenge to mushroom growers, the green mould disease of cultivated champignons. In, Curr. Res., Technol. Edu. Topics Appl. Microbiol. Microbial Biotechnol. (A. Mendez-Vilas ed.), 295–305.
Krupke O.A., Castle A.J., Rinker D.L., 2003. The North American mushroom competitor, Trichoderma aggressivum f. aggressivum, produces antifungal compounds in mushroom compost that inhibit mycelial growth of the commercial mushroom Agaricus bisporus. Mycol. Res. 107(12), 1467–1475.
Largeteau M.L., Savoie J.M., 2010. Microbially induced diseases of Agaricus bisporus, biochemical mechanisms and impact on commercial mushroom production. Appl. Microbiol. Biotechnol. 86, 63–73.
Lemke G., 1971. Mycelenzucht und Fruchtkorperproduktion des Kulturchampignons Agaricus bisporus (Lange) Sing. Gartenbauwissenschaft 36(18), 19–27.
Lemmers G., 2010. Trichoderma in Bulk Phase 3 (Part 1). Mushroom Business 40, 10–13.
Mamoun M., Savoie J.M., Olivier J.M., 2000a. Interaction between the pathogen Trichoderma harzianum Th2 and Agaricus bisporus in mushroom compost. Mycologia 92, 233–240.
Mamoun M., Iapicco R., Savoie J.M., Olivier J.M., 2000b. Green mould disease in France, Trichoderma harzianum Th2 and other species causing damage on mushroom farms. Mushroom Sci. 15, 625–632.
Mańka K., 1974. Zbiorowiska grzybów jako kryterium oceny wpływu środowiska na choroby roślin. Zesz. Probl. Post. Nauk. Roln. 160, 9–23.
Mumpuni A., Sharma H.S.S., Brown A.E., 1998. Effect of Metabolites Produced by Trichoderma harzianum Biotypes and Agaricus bisporus on Their Respective Growth Radii in Culture. Appl. Environ. Microbiol. 64(12), 5053–5056.
Ospina-Giraldo M.D., Royse D.J., Thon M.R., Chen X., Romaine C.P., 1999. Phylogenetic relationship of Trichoderma harzianum causing mushroom green mold in Europe and North America to other species of Trichoderma from world-wide sources. Mycologia 90, 76–81.
Pudełko K., Pyżalski S., 2010. Ocena przydatności technik molekularnych w diagnostyce zielonych pleśni w uprawach pieczarki (Agaricus bisporus). Progress in Plant Protection / Postępy w Ochronie Roślin 50(3), 1133–1139.
Samuels G.J., Dodd S.L., Gams W., Castelbury L.A., Petrini O., 2002. Trichoderma species associated with the green mold epidemic of commercially grown Agaricus bisporus. Mycologia 94(1), 146–170.
Savoie J.M., Iapicco R., Largeteau-Mamoun M.L., 2001. Factors influencing the competitive saprophytic ability of Trichoderma harzianum Th2 in mushroom (Agaricus bisporus) compost. Mycol. Res. 105, 1348–1356.
Savoie J.M., Mata G., 2003. Trichoderma harzianum metabolites pre-adapt mushrooms to Trichoderma aggressivum antagonism. Mycologia 95(2), 191–199.
Sharma H.S.S., Kilpatrick M., Ward F., Lyons G., Burns L., 1999. Colonisation of phase II compost by biotypes of Trichoderma harzianum and their effect on mushroom yield and quality. Appl. Microbiol. Biotechnol. 51, 572–578.
Sobieralski K., Siwulski M., Frużyńska-Jóźwiak D., Górski R., 2010. Impact of infection with Trichoderma aggressivum f. europaeum isolates on carpophore setting and yielding of Agaricus bisporus. Phytopathologia 55, 35–41.
Sobieralski K., Siwulski M., Frużyńska-Jóźwiak D., Górski R., 2009a. Impact of Trichoderma aggressivum f. europaeum Th2 on the yielding of Agaricus bisporus. Phytopathologia 53, 5–10.
Sobieralski K., Siwulski M., Górski R., Frużyńska-Jóźwiak D., 2009b. Porównanie rozwoju grzybni grzybów z rodzaju Trichoderma pochodzących z krajowych pieczarkarni. Progress in Plant Protection / Postępy w Ochronie Roślin 49(2), 723–726.
Sobieralski K., Salwin M., Nowak-Śliwocka B., 2000. Porównanie wzrostu grzybni w okrywie i wiązania owocników kultur jednozarodnikowych pieczarki dwuzarodnikowej Agaricus bisporus (Lange) Imbach. Roczn. AR Pozn., 323, Ogrodn. 31, cz. I, 485–489.
Szczech M., Staniaszek M., Habdes H., Uliński Z., Szymański J., 2008. Trichoderma spp. – the cause of green mold on Polish farms. Veg. Crops Res. Bull. 69, 105–114.
Tekiela A., 2005. Grzyby patogeniczne w uprawie pieczarki dwuzarodnikowej Agaricus bisporus (Lange) Imbach. Acta Agrobotanica 58(2), 189–196.
Williams J., Clarkson J.M., Mills P.R., Cooper R.M., 2003. Saprotrophic and Mycoparasitic Components of Aggressiveness of Trichoderma harzianum Groups toward the Commercial Mushroom Agaricus bisporus. Appl. Environ. Microbiol. 69(7), 4192–4199.
Błaszczyk L., Popiel D., Chełkowski J., Koczyk G., Samuels G.J., Sobieralski K., Siwulski M., 2011. Species diversity of Trichoderma in Poland. J. Appl. Genetics 52, 233–243.
Błażej J., Tekiela A., 2002. Występowanie grzybów pasożytniczych i konkurencyjnych dla pieczarki (Agaricus bisporus (Lange) Sing) w różnych podłożach i okrywie. Acta Sci. Pol. Hortorum Cultus 1(2), 33–41. Romaine C.P
Chen X., Ospina-Giraldo M.D., Wilkinson V., Royse D.J.,., 2003. Resistance of Pre- and Post-epidemic Strains of Agaricus bisporus to Trichoderma aggressivum f. aggressivum. Plant Disease 87, 1457–1461.
Fritsche G., 1972. Beispiel der Wirkung der Einsporkulturauslese als zuchterische Methode beim Kulturchampignon. Theor. Appl. Genet. 42, 62–64.
Frużyńska-Jóźwiak D., Siwulski M., Sobieralski K., Sas-Golak I., Błaszczyk L., 2011. Impact of Trichoderma isolates on the mycelium development of wild strains of Coprinus comatus (Müll.) S.F. Gray. J. Plant Protection Res. 51(2), 162–165.
Hatvani L., Antal Z., Manczinger L., Szekeres A., Druzhinina I.S., Kubicek C.P., Nagy A., Nagy E., Vágvölgyi C., Kredics L., 2007. Green mold diseases of Agaricus and Pleurotus spp. are caused by related but phylogenetically different Trichoderma species. Phytopathology 97, 532–537.
Hermosa M.R., Grondona I., Iturriaga E.A., Diaz-Minguez J.M., Castro C., Monte E., Garcia-Acha I., 2000. Molecular characterization and identification of biocontrol isolates of Trichoderma. Appl. Environ. Microbiol. 66, 1890–1898.
Kneebone L.R., Patton T.G., Schultz P.G., 1976. Improvement of the brown variety of Agaricus bisporusby single spore selection. Mushroom Sci. 9, 237–243.
Kredics L., Garcia Jimenez L., Naeimi S., Czifra D., Urban P., Manczinger L., Vágvölgyi C., Hatvani L., 2010. A challenge to mushroom growers, the green mould disease of cultivated champignons. In, Curr. Res., Technol. Edu. Topics Appl. Microbiol. Microbial Biotechnol. (A. Mendez-Vilas ed.), 295–305.
Krupke O.A., Castle A.J., Rinker D.L., 2003. The North American mushroom competitor, Trichoderma aggressivum f. aggressivum, produces antifungal compounds in mushroom compost that inhibit mycelial growth of the commercial mushroom Agaricus bisporus. Mycol. Res. 107(12), 1467–1475.
Largeteau M.L., Savoie J.M., 2010. Microbially induced diseases of Agaricus bisporus, biochemical mechanisms and impact on commercial mushroom production. Appl. Microbiol. Biotechnol. 86, 63–73.
Lemke G., 1971. Mycelenzucht und Fruchtkorperproduktion des Kulturchampignons Agaricus bisporus (Lange) Sing. Gartenbauwissenschaft 36(18), 19–27.
Lemmers G., 2010. Trichoderma in Bulk Phase 3 (Part 1). Mushroom Business 40, 10–13.
Mamoun M., Savoie J.M., Olivier J.M., 2000a. Interaction between the pathogen Trichoderma harzianum Th2 and Agaricus bisporus in mushroom compost. Mycologia 92, 233–240.
Mamoun M., Iapicco R., Savoie J.M., Olivier J.M., 2000b. Green mould disease in France, Trichoderma harzianum Th2 and other species causing damage on mushroom farms. Mushroom Sci. 15, 625–632.
Mańka K., 1974. Zbiorowiska grzybów jako kryterium oceny wpływu środowiska na choroby roślin. Zesz. Probl. Post. Nauk. Roln. 160, 9–23.
Mumpuni A., Sharma H.S.S., Brown A.E., 1998. Effect of Metabolites Produced by Trichoderma harzianum Biotypes and Agaricus bisporus on Their Respective Growth Radii in Culture. Appl. Environ. Microbiol. 64(12), 5053–5056.
Ospina-Giraldo M.D., Royse D.J., Thon M.R., Chen X., Romaine C.P., 1999. Phylogenetic relationship of Trichoderma harzianum causing mushroom green mold in Europe and North America to other species of Trichoderma from world-wide sources. Mycologia 90, 76–81.
Pudełko K., Pyżalski S., 2010. Ocena przydatności technik molekularnych w diagnostyce zielonych pleśni w uprawach pieczarki (Agaricus bisporus). Progress in Plant Protection / Postępy w Ochronie Roślin 50(3), 1133–1139.
Samuels G.J., Dodd S.L., Gams W., Castelbury L.A., Petrini O., 2002. Trichoderma species associated with the green mold epidemic of commercially grown Agaricus bisporus. Mycologia 94(1), 146–170.
Savoie J.M., Iapicco R., Largeteau-Mamoun M.L., 2001. Factors influencing the competitive saprophytic ability of Trichoderma harzianum Th2 in mushroom (Agaricus bisporus) compost. Mycol. Res. 105, 1348–1356.
Savoie J.M., Mata G., 2003. Trichoderma harzianum metabolites pre-adapt mushrooms to Trichoderma aggressivum antagonism. Mycologia 95(2), 191–199.
Sharma H.S.S., Kilpatrick M., Ward F., Lyons G., Burns L., 1999. Colonisation of phase II compost by biotypes of Trichoderma harzianum and their effect on mushroom yield and quality. Appl. Microbiol. Biotechnol. 51, 572–578.
Sobieralski K., Siwulski M., Frużyńska-Jóźwiak D., Górski R., 2010. Impact of infection with Trichoderma aggressivum f. europaeum isolates on carpophore setting and yielding of Agaricus bisporus. Phytopathologia 55, 35–41.
Sobieralski K., Siwulski M., Frużyńska-Jóźwiak D., Górski R., 2009a. Impact of Trichoderma aggressivum f. europaeum Th2 on the yielding of Agaricus bisporus. Phytopathologia 53, 5–10.
Sobieralski K., Siwulski M., Górski R., Frużyńska-Jóźwiak D., 2009b. Porównanie rozwoju grzybni grzybów z rodzaju Trichoderma pochodzących z krajowych pieczarkarni. Progress in Plant Protection / Postępy w Ochronie Roślin 49(2), 723–726.
Sobieralski K., Salwin M., Nowak-Śliwocka B., 2000. Porównanie wzrostu grzybni w okrywie i wiązania owocników kultur jednozarodnikowych pieczarki dwuzarodnikowej Agaricus bisporus (Lange) Imbach. Roczn. AR Pozn., 323, Ogrodn. 31, cz. I, 485–489.
Szczech M., Staniaszek M., Habdes H., Uliński Z., Szymański J., 2008. Trichoderma spp. – the cause of green mold on Polish farms. Veg. Crops Res. Bull. 69, 105–114.
Tekiela A., 2005. Grzyby patogeniczne w uprawie pieczarki dwuzarodnikowej Agaricus bisporus (Lange) Imbach. Acta Agrobotanica 58(2), 189–196.
Williams J., Clarkson J.M., Mills P.R., Cooper R.M., 2003. Saprotrophic and Mycoparasitic Components of Aggressiveness of Trichoderma harzianum Groups toward the Commercial Mushroom Agaricus bisporus. Appl. Environ. Microbiol. 69(7), 4192–4199.
Krzysztof Sobieralski
Poznań University of Life Sciences
Poznań University of Life Sciences
Marek Siwulski
Poznań University of Life Sciences
Poznań University of Life Sciences
Lidia Błaszczyk
Institute of Plant Genetics, Polish Academy of Science, Poznań
Institute of Plant Genetics, Polish Academy of Science, Poznań
Dorota Frużyńska-Jóźwiak
Poznań University of Life Sciences
Poznań University of Life Sciences
Jolanta Lisiecka
Poznań University of Life Sciences
Poznań University of Life Sciences
Licencja
Artykuły są udostępniane na zasadach CC BY 4.0 (do 2021 r. na zasadach CC BY-NC-ND 4.0 międzynarodowe).
Przysłanie artykułu do redakcji oznacza, że nie był on opublikowany wcześniej i nie jest rozpatrywany do publikacji gdzie indziej.
Autor podpisuje oświadczenie o oryginalności dzieła, wkładzie poszczególnych osób i źródle finansowania.
