EFFECT OF TIME AND INFUSION TEMPERATURE DRIED LEAVES DANDELION (Taraxacum officinale F.H. Wiggers coll.) ON THE CONTENT OF ANTIOXIDANT COMPOUNDS
Kałwa Klaudia
Katedra Analizy i Oceny Jakości Żywności, Uniwersytet Przyrodniczy w Lublinie, ul. Skromna 8, 20-704 LublinKamil Wilczyński
Katedra Inżynierii i Maszyn Spożywczych, Uniwersytet Przyrodniczy w Lublinie, ul. Głęboka 28, 20-612 LublinAbstract
The aim of the study was to determine the content of polyphenolic compounds, including flavonoids in infusions of dandelion leaves (Taraxacum officinale) depending on the time and the temperature of infusion and determination of antioxidant activity for the infusions tested. Samples of dried dandelion leaves about the weight of 2 g (±0.0001 g) were filled with deionized water (100 ml) at an initial temperature of 100°C, 80°C and 50°C. Determination of polyphenol content was carried out using Folin−Ciocalteu reagent. The flavonoid content expressed as epicatechin was determined spectrophotometrically. Antioxidant activity was determined using synthetic DPPH radical. It was found that the content of polyphenols expressed as gallic acid ranges between 14.18 mg/100 ml ± 0.07 and 37.52 mg/100 ml ± 0.04 depended on the initial temperature and infusion time. A similar relationship was found when the flavonoid content were within the range from 9.61 mg/100 ml ± 0.02 to 25.26 mg/100 ml ± 0.2. The ability to reduce the DPPH antioxidant radical ranged from 38.24% to 62.05%. The results confirm the high dependency of the content of phenolic
compounds in the infusions of dandelion leaves on the time and temperature of infusion.
Keywords:
dandelion, polyphenols, antioxidant properties, DPPH, health propertiesReferences
Astafieva, A.A., Enyenihi, A.A., Rogozhin, E.A., Kozlov, S.A., Grishin, E.V., Odintsova, T.I., Zubarev R.A., Egorov, T.A. (2015). Novel proline-hydroxyproline glycopeptides from the dandelion (Taraxacum officinale Wigg.) flowers: de novo sequencing and biological activity. Plant Sci., 238, 323−329.
Brand-Williams, W., Cuvelier, M.E., Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci. Technol., 28, 25−30.
Bruckert, E., Giral, P., Ratziu, V., Poynard, T., Chapman, M.J., Opolon, P. (2002). A constellation of cardiovascular risk factors is associated with hepatic enzyme elevation in hyperlipidemic patients. Metabolism, 51, 1071–1076.
Chatterjee, S.J., Ovadje, P., Mousa, M., Hamm, C., Pandey, S. (2011). The efficacy of dandelion root extract in inducing apoptosis in drug-resistant human melanoma cells. Evid. Based Complement. Alternat. Med., 129045. DOI: 10.1155/2011/129045
Choi, U.K., Lee, O.H., Yim, J.H., Cho, C.W., Rhee, Y.K., Lim, S.I., Kim, Y.C. (2010). Hypolipidemic and antioxidant effects of dandelion (Taraxacum officinale) root and leaf on cholesterol-fed rabbits. Int. J. Mol. Sci., 2, 11(1), 67–78.
Cieszyńska, A., Michocka, K., Wieczorek, D., Zieliński, R. (2011). Wpływ czasu ekstrakcji na aktywność przeciwrodnikową naparów herbaty zielonej. Probl. Hig. Epidemiol., 92(4), 872–875.
Gheribi, E. (2011). Związki polifenolowe w owocach i warzywach. Med. Rodz., 4, 111–115.
Gomez, M.K., Singh, J., Acharya, P., Jayaprakasha, G.K., Patil, B.S. (2018). Identification and quantification of phytochemicals, antioxidant activity, and bile acid-binding capacity of garnet stem dandelion (Taraxacum officinale). Food Sci., 83(6), 1569–1578.
Greenlee, H., Atkinson, C., Stanczyk, F.Z., Lampe, J.W. (2007). A pilot and feasibility study on the effects of naturopathic botanical and dietary interventions on sex steroid hormone metabolism in premenopausal women. Cancer Epidemiol. Biomarkers Prev., 16(8), 1601–1609.
Hong-Bin, W. (2014). Cellulase-assisted extraction and antibacterial activity of polysaccharides from the dandelion Taraxacum officinale. Carbohydr. Polym., 103, 140–142.
Ivanov, I.G. (2014). Polyphenols content and antioxidant activities of Taraxacum officinale F.H. Wigg (Dandelion) Leaves. Int. J. Pharmacogn. Phytochem. Res., 15, 6(4), 889–893.
Ivanova, D., Gerova, D., Chervenkov, T., Yankova, T. (2005). Polyphenols and antioxidant capacity of Bulgarian medicinal plants. J. Ethnopharmacol., 96, 145–150.
Karadeniz, F., Burdurlu, H.S., Koca, N., Soyer, Y. (2005). Antioxidant activity of selected fruits and vegetables grown in Turkey. Turk. J. Agric. For., 29(4), 297–303.
Kasianningsih, S., Rivanti, E., Pratama, R.H., Pratama, N.R., Ikawati, M., Meiyanto, E. (2011). Taraxacum officinale leaves ethanolic extract as immunostimulatory agent for reducing side effect of doxorubicin in Sprague dawley rats. Indones. J. Cancer Chemoprev., 2(1), 135–140.
Koo, H.N., Hong, S.H., Song, B.K. (2004). Taraxacum officinale induces cytotoxicity through TNF-alpha and IL-1alpha secretion in Hep G2 cells. Life Sci., 74(9), 1149–1157.
Kratchanova, M., Denev, P., Ciz, M., Lojek, A., Mihailov, A. (2010). Evaluation of antioxidant activity of medicinal plants containing polyphenol compounds. Comparison of two extraction systems. Acta Biochim. Pol., 57(2), 229–234.
Lis, B., Grabek-Lejko, D. (2016). Mniszek lekarski (Taraxacum officinale) – potencjalne właściwości prozdrowotne. Nauka Przyr. Technol., 10(3), 1–15.
Menke, K., Schwermer, M., Felenda, J., Beckmann, C., Stintzing, F., Schramm, A., Zuzak, T.J. (2018). Taraxacum officinale extract shows antitumor effects on pediatric cancer cells and enhance mistletoe therapy. Complement. Ther. Med., https://doi.org/10.1016/j.ctim.2018.03.005
Okwu, D.E. (2001). Evaluation of the chemical composition of indigenous spices and flavoring agents. Global J. Pure Appl. Sci., 7(3), 455−459.
Perucka, I. (2001). Skład chemiczny liści herbaty. Biul. Magnezoil., 6(3), 443−451.
Rodino, S., Butu, A., Butu, M., Cornea, P.C. (2015). Comparative studies on antibacterial activity of licorice, elderberry and dandelion. Digest J. Nanomater. Biostruct., 10(3), 947−955.
Sigstedt, S.C., Hoosten, C.J., Callewaert, M.C., Jenkins, A.R., Romero, A.E., Pullin, M.J., Singleton, V.L., Rosi, J.A. (1965). Colorimetry of total phenolics with phosphomolybdic phosphotungstic reagents. Am. J. Enol. Viticult., 16, 144−158.
Witzum, J.L., Steinberg, D. (2001). The oxidative modification hypothesis of atherosclerosis: Dose it hold for humans? Trends Cardiovasc. Med., 1, 93–102.
You, Y., Yoo, S., Yoon, H.G., Park, J., Lee, Y.H., Kim, S., Oh, K.T., Lee, J., Cho, H.Y., Jun W. (2010). In vitro and in vivo hepatoprotective effects of the aqueous extract from Taraxacum officinale (dandelion) root against alcohol-induced oxidative stress. Food Chem. Toxicol., 48(6), 1632–1637.
Katedra Analizy i Oceny Jakości Żywności, Uniwersytet Przyrodniczy w Lublinie, ul. Skromna 8, 20-704 Lublin
Katedra Inżynierii i Maszyn Spożywczych, Uniwersytet Przyrodniczy w Lublinie, ul. Głęboka 28, 20-612 Lublin
License
Articles are made available under the CC BY-NC-ND 4.0 (recognition by authorship, non-commercial use, no dependent works).
The author signs a statement on the originality of the work and the contribution of individuals.
Submission of the paper implies that it has not been published previously, that it is not under consideration for publication elsewhere.
