Abstrakt
Grelina (GHRL) i białka rozprzęgające UCP2, UCP3 pełnią funkcjonalną rolę w globalnym metabolizmie ciała, wzroście i otłuszczeniu, a takŜe w organoleptycznej jakości mięsa. Celem badań była analiza homologii między regionami chromosomów człowieka zawierających loci GHRL, UCP2, UCP3 a odpowiadającymi im fragmentami genomu dzika (Sus scrofa scrofa) przy wykorzystaniu techniki FISH. Do międzygatunkowych hybrydyzacji in situ z chromosomami dzika – kariotyp 36,XY,rob(15;17) – wykorzystano dwie komercyjne ludzkie sondy (Vysis), specyficzne dla regionów autosomów 3 i 11 pary (HSA3p25-26 i HSA11q13). Ustalono następujące fizyczne lokalizacje – gen GHRL zidentyfikowano u dzika w autosomalnym interstycjalnym regionie SSC13q31-32, a geny UCP2 i UCP3, ze względu na bliskie sąsiedztwo, wymapowano w tym samym regionie chromosomu SSC9p21-24. Międzygatunkowe hybrydyzacje in situ potwierdziły konserwatyzm grup sprzęŜeniowych oraz wysoki stopień homologii regionów chromosomowych zawierających loci GHRL, UCP2 i UCP3 w genomach człowieka, świni domowej i dzika.
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