Abstract
The knowledge of life requirements of phytopathogenic fungi allows to provide the periods of their mass occurrence on plant plantations and determine the optimal conditions for their isolation and culture on artificial media. Areas of occurrence of P. diachenii on caraway plants indicate the thermophilic nature of the fungus although there is a lack of information about research on this topic. In the present study the growth and development of one-spore cultures of six isolates of P. diachenii at the temperature: 0oC, 4oC, 10oC, 16oC, 22oC, 28oC, 33oC and 38oC on Czapek-Dox medium and also the growth and development of the same isolates in eight agar media at the temperature 25°C were examined. It was shown that the optimum temperature for the growth and sporulation of most
isolates of P. diachenii and for the secretion of mature conidia from conidioma ranged 22oC to 28oC. Based on the nature of growth and development of P. diachenii on the examined media, Czapek-Dox and mineral ones as slightly acidic, and possibly malt extract should be recommend for isolation of the fungus from the plant tissue. PDA and Czapek-Dox media were considered the most suitable for diagnostic purposes due to the formation of characteristic macroscopic and microscopic features on these substrates. These substrates with fragments of carnation leaves should be recommended for the stimulation of fungal sporulation.
References
Boerema G.H., Gruyter de J Noordeloos M.E., Hamers M.E.C., 2004. Phoma identification manual differentiation on specific and intra-specific taxa in culture. CAB Publishing.
Castillo-Pando M.S., Nair N.G., Emmett R.W., Wicks T.J., 1997. Inhibition in pycnidial viability of Phomopsis viticola on canes in situ as an aid to reducing inoculum potential of cane and leaf blight disease of grapevines. Austral. Plant Pathol., 26, 21–25.
Cavanni P., Fantuz F., Ponti J., 1987. Le malattie crittogarmiche del legno della vite. Informatore Fitopatologico 37, 1, 27–34.
Farr D.F., Bills G.F., Chamuris G.P., Rossman A.Y., 1995. Fungi on plants and plant products in the United States. St. Paul, Minnesota USA.
Gabler J., Ehrig F., 2000. Phomopsis diachenii Sacc., ein aggressiver Krankheitserreger an Kümmel (Carum carvi L.) – Erstnachweis für Deutschland./Phomopsis diachenii Sacc., an important pathogen on caraway (Carum carvi L.) – first detection for Germany. Z. Arzn. Gew. Pfl., 1, 36–39.
Król E., 2007. Phomopsis viticola Sacc. jako patogen winorośli na świecie i w Polsce./ Phomopsis viticola Sacc. the pathogen of Vitis spp. on the World and in Poland. Post. Nauk Roln. 4, 85–96.
Król E., 2006a. Grzyby zasiedlające zdrowe łozy winorośli (Vitis spp.) w wybranych szkółkach./ Fungi inhabiting healthy grapevine canes (Vitis spp). Acta Agobot. 59, 2, 163–173.
Król E., 2006b. Fungi inhabiting decaying grapevine (Vitis spp.) cuttings. J. Plant Protection Res., 46, 4, 353–358.
Król E., 2005. Influence of some chemicals on the viability of Phomopsis viticola Sacc. Spores. J. Plant Protection Res., 45, 3, 195–204.
Machowicz-Stefaniak Z., 2009. The occurrence and biotic activity of Phomopsis diachenii Sacc. Acta Agrobot., 62 (2), 125 – 135.
Machowicz-Stefaniak Z., 1993. Phomopsis viticola Sacc. (Sphaeropsidales, Deuteromycotina) nowy w Polsce patogen pędów winorośli./Phomopsis viticola Sacc. (Sphaeropsidales, Deuteromycotina) new pathogen of grape-vine canes in Poland. Acta Mycologica 28, 157–160.
Machowicz-Stefaniak Z., Kuropatwa E., 1993. Pathogenicity of Phomopsis viticola Sacc. for grape-vine (Vitis vinifera L.) under foil tunnel conditions. Phytopathol. Pol., 5, 67–72.
Machowicz-Stefaniak Z., Zalewska E., 2008. Biodiversity of fungi colonizing different parts of caraway (Carum carvi L.). EJPAU, Horticulturae 11, 1, www.ejpau.media.pl
Machowicz-Stefaniak Z., Zalewska E., 2004. Grzyby zagrażające uprawie wybranych gatunków ziół z rodziny Apiaceae w południowo-wschodniej Polsce./ Fungi threatening cultivation of chosen herbs species from Apiaceae family in south-east Poland. Folia Univ. Agric. Stetin, Agricultura, 239 (95), 223–228.
Marcinkowska J., 2003. Oznaczanie rodzajów grzybów ważnych w patologii roślin. /Marking of fungi genera significant in plant pathology. Fundacja Rozwój SGGW, Warszawa.
Mármureanu M., Dumitru C., Oprea M., 1990. Bioekologia, transmiterea Si combaterea ciupercii Phomopsis viticiola Sacc. Horticultura 2, 26–30.
Rodeva R., Gabler J., 2004. First report of Phomopsis diachenii in Bulgaria. Mycologica Balcanica, 1, 153–157.
Udayanga D., Liu X., McKenzie E.H.C., Chukeatirote E., Bahkali A.H.A., Hyde K.D., 2011. The genus Phomopsis: biology, applications, species concepts and names of common phytopathogens. Fungal Diversity 50, 189–225.
Uecker F.A., 1988. A Word list of Phomopsis names with notes on nomenclature, morphology and biology. Mycol. Mem. 13, 231.
Zalewska E., 2012. Growth and sporulation of Septoria carvi Syd. in different culture conditions. Acta Sci. Pol. Hortorum Cultus 11(1), 93–107.
Zimowska B., 2010. Characteristics of Phomopsis sclareae obtained from sage (Salvia officinalis L.). Biologia, Section Botany, 65/4, 603–608.
Downloads
Download data is not yet available.
