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ASPHC Baner

Vol. 25 No. 3 (2026):

Research paper

Biotechnological modulation of metabolite profiles in Ganoderma resinaceum cultivated on agricultural residues

DOI: https://doi.org/10.24326/asphc.2026.5655
Submitted: 12 January 2026
Published: 22.06.2026

Abstract

Ganoderma resinaceum is a medicinal macrofungus recognised for its diverse pharmacologically active metabolites, including amino acids, organic acids, and polysaccharides with antioxidant, anti-inflammatory, and anticancer potential. This study aimed to elucidate how substrate formulation based on agricultural residues can modulate the metabolic composition of G. resinaceum under controlled cultivation conditions. Nine substrate mixtures were prepared using chickpea (Cicer arietinum), pea (Pisum sativum), and poppy (Papaver somniferum) stalks, as well as corncobs (Zea mays), in combination with beech sawdust and wheat bran. The fruiting bodies were analysed for amino acid, organic acid, and sugar profiles using high-performance liquid chromatography (HPLC). The results demonstrated that substrate composition markedly influenced metabolite accumulation. Chickpea- and pea-based substrates promoted the biosynthesis of key amino acids, whereas poppy stalk affected organic acid balance, and corncob formulations modified sugar metabolism. The findings indicate that metabolite production in G. resinaceum is strongly substrate-dependent, reflecting both nutrient availability and biochemical adaptability of the fungus. Overall, the study highlights a sustainable biotechnological approach to enhance bioactive metabolite production through tailored substrate design. The outcomes provide a foundation for future optimisation of G. resinaceum cultivation toward pharmaceutical and nutraceutical applications.

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