Skip to main navigation menu Skip to main content Skip to site footer
ASPHC Baner

Vol. 19 No. 5 (2020)

Articles

DO JASMONIC ACID AND ACTIVATED CHARCOAL INCREASE THE in vitro DEVELOPMENT OF ORANGE CARROT (Daucus carota L.) AND PURPLE CARROT (Daucus carota L. ssp. sativus var. atrorubens Alef.)?

DOI: https://doi.org/10.24326/asphc.2020.5.12
Submitted: 17 July 2019
Published: 29.10.2020

Abstract

Present study aimed to reveal the effects of jasmonic acid and activated charcoal on in vitro carrot plantlet regeneration by using Murashige and Skoog (MS) medium supplemented with BAP, NAA, activated charcoal and jasmonic acid at various concentrations. To serve the purpose, in vitro carrot seed germination, shoots, cotyledons, and first leaves formation of orange and purple carrot plantlets were investigated. During the experiments, root size, weight, and size of petiole diameter, hyperhydricity and callus formation rate were recorded. Experimental results revealed that combination of jasmonic acid and activated charcoal in medium had a positive effect especially on the first stage of developmental processes such as seed swelling and germination, cotyledon and first leaf formation as well as having positive effects on above-ground internode elongation, petiole and plantlet height.

References

  1. Ahmadi, B., Shariatpanahi, M.E., da Silva, J.A.T. (2014). Efficient induction of microspore embryogenesis using abscisic acid, jasmonic acid and salicylic acid in Brassica napus L. Plant Cell Tiss. Organ Cult., 116, 343–351. DOI: 10.1007/s11240-013-0408-x
  2. Baktır, İ. (2015). Jasmonateler (Yasemin Asitleri). Hormonlar, Bitki Gelişim Düzenleyicileri, Özellikleri ve Tarımda Kullanımları [Jasmonates (Jasmine Acids). Hormones, Plant Growth Regulators, Properties and Uses in Agriculture]. İstanbul, Türkiye: Hasad Yayıncılık, sf. 90–92 [in Turkish].
  3. Berger, S., Bell, E., Mullet, J.E. (1996). Two methyl jasmonate-insensitive mutants show altered expression of AtVsp in response to methyl jasmonate and wounding. Plant Physiol., 111, 525–531. DOI: 10.1104/pp.111.2.525
  4. Creelman, R.A., Mullet, J.E. (1997). Biosynthesis and action jasmonates in plants. Ann. Rev. Plant Physiol. Plant Mol. Biol., 48, 355–381.
  5. Çavusoglu, K., Kabar, K. (2006). Does Jasmonic Acid Prevent The Germination of Barley Seeds? SDÜ Fen Edebiyat Fakültesi Fen Dergisi (E-Dergi), 1(12–2), 35–41.
  6. Çavusoglu, K., Kabar, K., Kılıç, S. (2007). Effects of Some Plant Growth Regulators on Jasmonic Acid Induced Inhibition of Seed Germination and seedling Growth of Barley. SDÜ Fen Edebiyat Fakültesi. Fen Dergisi (E-Dergi), 2(1), 53–59.
  7. Dereli, U. (2010). Siyah Havuç Suyu Konsantresi Üretimi ve Depolanması Sürecinde Fenolik Maddelerdeki Değişimler ve Bu Değişimlerin Antioksidan Aktivite ile İlişkisi. Yüksek Lisans Tezi. Ankara Üniversitesi, Fen Bilimleri Enstitüsü.
  8. Gaspar, T., Kevers, C., Penel, C., Greppin, H., Reid, D.M., Thorpe, T.A. (1996). Plant Hormones and Plant Growth Regulators In Plant Tissue Culture. In vitro Cell. Dev. Biol.-Plant, 32, 272–289.
  9. Grebenstein, C., Kos, S.P., de Jong, T.J., Tamis, W.L.M., de Snoo, G.R. (2013). Morphological markers for the detection of introgression from cultivated into wild carrot (Daucus carota L.) reveal dominant domestication traits. Plant Biol., 15, 531–540.
  10. Hanci, F., Cebeci, E. (2012). Siyah Havuç (Daucus carota ssp. sativus var. atrorubens Alef.) Yetiştiriciliği. Tarım Türk Dergisi, 7, 36–40 [in Turkish].
  11. Jourdan, M., Gagné, S., Dubois-Laurent, C., Maghraoui, M., Huet, S., Suel, A., Hamama, L., Briard, M., Peltier, D., Geoffriau, E. (2015). Carotenoid content and root color of cultivated carrot: a candidate-gene association study using an original broad unstructured population. PLoS One, 10:e0116674, 1–19. DOI: 10.1371/journal.pone.0116674
  12. Kenar, S. (2013). Patateste (Solanum tuberosum L.) in vitro Mikrotuberizasyon Üzerine Jasmonik Asit – Giberellik Asit Etkileşiminin Araştırılması. Yüksek Lisans Tezi, Hacettepe Üniversitesi, Ankara, Turkey [in Turkish].
  13. Kepczynski, J., Bialecka, B., Kepczynska, E. (1999). Ethylene biosynthesis in Amaranthus caudatus seeds in response to methyl jasmonate. J. Plant Growth Regul., 28, 59–65. DOI: 10.1023/A:1006201625637
  14. Koda, Y., Kikuta, Y., Takazi, H., Tsujino, Y., Sakamura, S., Yoshihara, T. (1991). Potato tuber-inducing activities of jasmonic acid and related compounds. Phytochemistry, 30(5), 1435–1438. DOI: 10.1016/0031-9422(91)84180-Z
  15. Koda, Y., Kikuta, Y. (1991). Possible involvement of jasmonic acid in tuberization in yam plants. Plant Cell Physiol., 32, 629–633.
  16. Koda, Y., Kikuta, Y. (2001). Effects of jasmonates on in vitro tuberization in several potato cultivars that differ greatly in maturity. Plant Prod. Sci., 4(1), 66–70. DOI: 10.1626/pps.4.66
  17. Lorenzo, O., Solano, R. (2005). Molecular players regulating the jasmonate signalling network. Curr. Opin. Plant Biol., 8, 532–540. DOI: 10.1016/j.pbi.2005.07.003.
  18. Luby, C.H., Maeda, H.A., Goldman, I.L. (2014). Genetic and phenological variation of tocochromanol (vitamin E) content in wild (Daucus carota L. var. carota) and domesticated carrot (D. carota L. var. sativa). Hortic. Res., 1(15), 1–6. DOI: 10.1038/hortres.2014
  19. Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15, 473–97.
  20. Nowacka, M., Wedzik, M. (2016). Effect of ultrasound treatment onmicrostructure, colour and carotenoid content in fresh and dried carrot tissue. Appl. Acoust., 103, 163–171. DOI: 10.1016/j.apacoust.2015.06.011
  21. Pelacho, A.M., Mingo-Castel, A.M. (1991). Jasmonic acid induces tuberization of potato stolons cultured in vitro. Plant Physiol., 97, 1253–1255. DOI: 10.1104/pp.97.3.1253
  22. Parthier, B. (1991). Jasmonates, new regulators of plant growth and development: Many facts and few hypotheses of their action. Bot. Acta, 104, 446–454. DOI: 10.1111/j.1438-8677.1991.tb00257.x
  23. Rathinapriya, P., Satish, L., Rameshkumar, R., Pandian, S., Rency, A.S., Ramesh, M. (2019). Role of activated charcoal and amino acids in developing an efficient regeneration system for foxtail millet (Setaria italica (L.) Beauv.) using leaf base segments. Physiol. Mol. Biol. Plants, 25(2), 533–548. DOI: 10.1007/s12298-018-0619-z
  24. Ravnikar, M., Gogala, N. (1990). Regulation of potato meristem development by jasmonic acid in vitro. J. Plant Growth Regul., 9, 233–236. DOI: 10.1007/BF02041968
  25. Ravnikar, M., Vilhar, B., Gogala, N. (1992). Stimulatory effects of jasmonic acid on potato node and protoplast culture. J. Plant Growth Regul., 11(1), 29–33. DOI: 10.1007/BF00193840
  26. Ravnikar, M., Žel, J., Plaper, I., Špacapan, A. (1993). Jasmonic acid stimulates shoot and bulb formation of garlic in vitro. J. Plant Growth Reg., 12(2), 73–77. DOI: 10.1007/BF00193236
  27. Sarkar, D., Pandey, S.K., Sharma, S. (2006). Cytokinins antagonize the jasmonates action on the regulation of potato (Solanum tuberosum) tuber formation in vitro. Plant Cell, Tissue Organ Cult., 87, 285–295. DOI: 10.1007/s11240-006-9166-3
  28. Teixeira da Silva, J.A. (2012). Jasmonic Acid, but not Salicylic Acid, Improves PLB Formation of Hybrid Cymbidium. Plant Tissue Cult. Biotech., 22(2), 187–192. DOI: 10.3329/ptcb.v22i2.14209
  29. Thomas, D. (2008). The role of activated charcoal in plant tissue culture. Biotechnol. Adv., 26, 618–631. DOI: 10.1016/j.biotechadv.2008.08.003
  30. Tsai, S.J., Juengel, J.L., Wiltbank, M.C. (1997). Hormonal regulation of monocyte chemoattractant protein-1 messenger ribonucleic acid expression in corpora lutea. Endocrinology, 138, 4517–4520. DOI: 10.1210/endo.138.10.5577
  31. Wang, G.L., Que, F., Xu, Z.S., Wang, F., Xiong, A.S. (2017). Exogenous gibberellin enhances secondary xylem development and lignification in carrot taproot. Protoplasma, 254, 839–848. DOI: 10.1007/s00709-016-0995-6
  32. Yazawa, K., Takahata, K., Kamada, H. (2004). Isolation of the gene encoding carrot leafy cotyledon1 and expression analysis during somatic and zygotic embryogenesis. Plant Physiol. Biochem., 42(3), 215–223. DOI: 10.1016/j.plaphy.2003.12.003
  33. Wilen, R.W., Mandel, R.M., Pharis, R.P., Holbrook, L.A., Moloney, M.M. (1990). Effects of abscisic acid and high osmoticum on storage protein gene expression in microspore embryos of Brassica napus. Plant Physiol., 94, 875–881. DOI: 10.1104/pp.94.3.875

Downloads

Download data is not yet available.

Similar Articles

<< < 12 13 14 15 16 17 18 19 20 21 > >> 

You may also start an advanced similarity search for this article.