Abstract
Plant regeneration through the tissue culture techniques would be a excellent alternative for improving the quality and faster production of Asparagus species. The number of reports on application of biotechnologi-cal methods (such as direct organogenesis, indirect organogenesis and somatic embriogenesis) for ornamen-tal asparagus are scarce in comparison to edible asparagus. The aim of this study was to verify the effec-tiveness of chemical and physical factors on organogenetic response of Asparagus setaceus ‘Pyramidalis’ explants. The results showed that nodes cultured on MS medium supplemented on IAA (1.71 μM) and BA (13.32 μM) gave highest number of shoots per explant (14 – in the fourth passage). Rhizogenesis were achieved on MS medium with IBA (2.45 μM) and ancymidol (2.88 μM). Finally, 90% of rooted shoots (microcuttings) survived. The fastest increase in callus tissue on internodal explants were observed on MS medium with addition of TDZ (9.08 μM) and adenine sulphate (10.86 μM).
References
Araki, H., Watanabe, S., Harada, T., Yakuwa, T. (1996). Aerial crown-like body formation through nodal seg-ment culture in Asparagus. Proceedings VIII Int. Sym. on Asparagus. Acta Hort., 415, 209–214.
Baker, J.G. (1875). Flora of Australia online. ABRS, ©Commonwealth of Australia. 1994. Retrieved 2009-07-29.
Bansal, R.K., Menzies, S.A., Broadhurst, P.G. (1986). Screening of Asparagus species for resistance to Stem-phylium leaf spot. New Zealand J. Agric. Res., 29, 539–545.
Benson, E. (2000). In vitro plant recalcitrance: an introduc-tion. In Vitro Cell. Dev. Biol. Plant, 36, 141–148.
Bezona, N., Hensley, D., Yogi, J., Tavares, J., Rauch, F., Iwata, R., Kellison, M., Wong, M., Clifford, P. (2009). Salt and wind tolerance of landscape plants for Hawaii. Landscape, L–13, 1–9.
Chang, D.C., Peng, K.H. (1996). Phloroglucinol and tryp-tone enhance in vitro rooting and survival rate of aspar-agus nodal sections. Proceedings VIII Int. Sym. on As-paragus. Acta Hort., 415, 411–417.
Dahlgren. R.M.T., Clifford. H.T., Yeo. P.F. (1985). The families of the monocotyledons. Springer, Berlin.
Dasgupta, C.N., Mukhopadhyay, M.J., Mukhopadhyay, S. (2007). Somatic embryogenesis in Asparagus densiflo-rus (Kunth) Jessop cv Sprengeri. J. Plant Biochem. Bi-otech., 16(2), 145–149.
Desjardins Y., Tiessen H., Harney P., M. (1987). The effect of sucrose and ancymidol on the in vitro rooting of nodal section of Asparagus. HortScience, 22(1), 131–133.
Flora of Australia 1994. Online. ABRS, http://www.environment.gov.au/biodiversity/abrs/online-resources/flora/main/
Fonnesbech, A., Fonnesbech, M, Bredmose, N. (1977 a). Development of Asparagus plumosus shoot tips grown in vitro. Physiol. Plant., 40, 73–76.
Fonnesbech, A., Fonnesbech, M., Bredmose, N. (1977 b). Growth and development of Asparagus plumosus shoot tips in vitro. Acta Hort., 78, 287.
Kanno, A., Yokoyama, J. (2011). Wild crop relatives: Genome and breeding resources. Springer Verlag Ber-lin Heidelberg, 23–42.
Kar, D.K., Sen, S. (1985). Propagation of Asparagus race-mosus through tissue culture. Plant Cell Tiss. Org. Cult., 5, 89–95.
Li, B., Wolyn, D.J. (1997). Interactions of ancymidol with sucrose and α-naphtaleneacetic acid in promoting as-paragus (Asparagus offcinalis L.) somatic embryogene-sis. Plant Cell Rep., 16, 879–883.
Linsmayer, E.M., Skoog, F. (1965). Organic growth factor requirements of tobacco cultures. Physiol. Plant., 18, 100–127.
Loo, S.W. (1945). Cultivation of excised stem tips of as-paragus in vitro. Am. J. Bot., 32, 13–17.
Loo, S.W. (1946). Further experiments on the culture of excised asparagus stem tips in vitro. Am. J. Bot., 33, 156–159.
McCollum, G.D. (1988). Asparagus densiflorus cultivars Sprengeri and Meyers cross-pollinations with A. offici-nalis and other species. Asparag. Newsl., 6, 1–10.
Mehta, S.R., Subramanian, R.B. (2005). Direct in vitro propagation of Asparagus adscendens Roxb. Plant Tiss. Cult., 15(1), 25–32.
Murashige, T., Skoog, F. (1962). A revised medium for the rapid growth and bioassays with tobacco tissue cul-tures. Physiol. Plant., 15, 473–497.
Nawaz, T., Hameed, M., Waqar-U-Nisa, Ahmad, M.S.A., Younis, A., Kanwal, H. (2012). Comparative anatomy of root and stem of some native and exotic Asparagus L. species. Pak. J. Bot., 44, 153–158.
Pant, K.K., Joshi, S.D. (2009). In vitro Multiplication of wild Nepalese Asparagus racemosus through shoots and shoot induced callus cultures. Botany Res. Inter-nat., 2, 88–93.
Patel, L.S., Patel, R.S. (2015). Rapid in vitro micro propaga-tion of Asparagus recemosus Willd. from nodal explants. Int. J. Curr. Microbiol. App. Sci., 4(5), 607–617.
Pindel, A. (2000). Przydatność różnych metod in vitro w rozmnażaniu szparagów ozdobnych. Zesz. Nauk. AR Kraków, 266, 1–69.
Rastogi, R., Sawheny, V.K. (1989). In vitro development of angiosperms floral buds and organs. Plant Cell Tiss. Org. Cult., 16, 145–174.
Regalado, J.J., Carmona-Martin, E., Castro, P., Moreno, R., Gil, J., Encina, C.L. (2015). Micropropagation of wild species of the genus Asparagus L. and their inter-specific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs. Plant Cell Tiss. Org. Cult., 121, 501–510.
Ren, J., Chen, W., Knaflewski, M. (2012). Factors affect-ing asparagus (Asparagus officinalis L.) root develop-ment in vitro. Acta Sci. Pol. Hortorum Cultus, 11(6), 107–118.
Rodriquez-Arcos, R.C., Smith, A.C., Waldron, K.W. (2002). Mechanical properties of green asparagus. J. Sci. Food Agric., 82, 293–300.
Sarabi, B., Almasi, K. (2010). Indirect organogenesis is useful for propagation of Iranian edible wild aspara-gus (Asparagus officinalis L.). Asian J. Agr. Sci., 2(2), 47–50.
Simpson, D.P. (1979). Casell’s Latin Dictionary. London.
Štajner, N., Bohanec, B., Javornik, B. (2002). Genetic variability of economically important Asparagus spe-cies as revealed by genome size analysis and rDNAITS polymorphism. Plant Sci. doi: 1016/SO168-9452(02)00039-0
Yang, H.J. (1977). Tissue culture technique developed for asparagus propagation. HortSci., 12, 140.
Ziv, M. (2005). Simple bioreactors for mass propagation of plants. Plant Cell Tiss. Org. Cult., 81, 277–285.
Ziv, M., Naor, V. (2006). Flowering of geophytes in vitro. Prop. Ornam. Plants, 6, 3–16.
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