Skip to main navigation menu Skip to main content Skip to site footer
ASPHC Baner

Vol. 21 No. 4 (2022)

Articles

Humulus lupulus L. (Hop) Based Silver Nanoparticles: Synthesis, Characterization and Enzyme Inhibition Effects

DOI: https://doi.org/10.24326/asphc.2022.4.2
Submitted: December 25, 2021
Published: 2022-08-31

Abstract

Silver nanoparticles (AgNPs) are well known to have antimicrobial activity, but very little is known about the effect of AgNPs on various enzyme activities. They (AgNPs) are valuable metal nanoparticles that exhibit exceptional properties compared to their bulk materials. Humulus lupulus L. (hops) is an important medicinal aromatic plant used in industry. It has many compounds such as phenolic, flavonoids, tannins, etc. In this study, green syntheses of Humulus lupulus L. based silver nanoparticles were performed. Accordingly, it was determined that HL-AgNPs gave maximum absorbance at approximately 450 nm and nanoparticle sizes ranged from 30.60 nm to 36.72 nm. The potential peaks of the prepared aqueous extract and HL-AgNPs were determined using FTIR-ATR. It was determined that the synthesized nanoparticles gave 2296.89 cm–1, 1161.05 cm–1, 1112.34 cm–1 peaks. Total phenolic content of HL-AgNPs was determined as 30.62 ±0.02 mg GAE/mL, and DPPH· radical scavenging activity IC50 value was determined as 4.4 ±0.01 mg/mL. Inhibitory effects of HL-AgNPs on α-amylase, α-glycosidase and urease enzymes were studied and IC50 values were determined as 3.10 ±0.01 mg/mL, 9.42 ±0.02 mg/mL and 0.76 ±0.01 mg/mL, respectively. The synthesized
Humulus lupulus L. based silver nanoparticles showed better biochemical activity than the prepared Humulus
lupulus L. aqueous extract. It is clear that it is possible to use HL-AgNPs obtained by green synthesis in various biomedical applications.

References

  1. Ali, S., Bacha, M., Shah, M.R., Shah, W., Kubra, K., Khan, A., Ahmad, M., Ali, M. (2020). Green synthesis of silver and gold nanoparticles using Crataegus oxyacantha extract and their urease inhibitory activities. Biotechnol. Appl. Biochem., 68(5), 992–1002. https://doi.org/10.1002/bab.2018 DOI: https://doi.org/10.1002/bab.2018
  2. Al-Yousef, H.M., Amina, M., Alqahtani, A.S., Alqahtani, M.S., Malik, A., Hatshan, M.R., Siddiqui, M.R.H., Khan, M., Shaik, M.R., Ola, M.S., Syed, R. (2020). Pollen bee aqueous extract-based synthesis of silver nanoparticles and evaluation of their anti-cancer and anti-bacterial activities. Processes, 8(5), 524. https://doi.org/10.3390/pr8050524 DOI: https://doi.org/10.3390/pr8050524
  3. Amin, M., Anwar, F., Janjua, M.R.S.A., Iqbal, M.A., Rashid, U. (2012). Green synthesis of silver nanoparticles through reduction with Solanum xanthocarpum L. berry extract: characterization antimicrobial and urease inhibitory activities against Helicobacter pylori. Int. J. Mol. Sci., 13(8), 9923–9941. DOI: https://doi.org/10.3390/ijms13089923
  4. Balciunaitiene, A., Viskelis, P., Viskelis, J., Streimikyte, P., Liaudanskas, M., Bartkiene, E., Zavistanaviciute, Zokaityte, E., Starkute, V., Ruzauskas M., Lele, V. (2021). Green synthesis of silver nanoparticles using extract of Artemisia absinthium L., Humulus lupulus L. and Thymus vulgaris L., physico-chemical characterization antimicrobial and antioxidant activity. Processes, 9(8), 1304. https://doi.org/10.3390/pr9081304 DOI: https://doi.org/10.3390/pr9081304
  5. Balan, K., Qing, W., Wang, Y., Liu, X., Palvannan, T., Wang, Y., Ma, Y., Zhang, Y. (2016). Antidiabetic activity of silver nanoparticles from green synthesis using Lonicera japonica leaf extract. Rsc Advances, 6(46), 40162–40168. DOI: https://doi.org/10.1039/C5RA24391B
  6. Bernfeld, P. (1955). Amylases alpha and beta. Methods Enzymol., 1, 149–158. http://dx.doi.org/10.1016/0076-6879(55)01021-5 DOI: https://doi.org/10.1016/0076-6879(55)01021-5
  7. Beykaya, M., Çağlar, A. (2016). Bitkisel özütler kullanılarak gümüş-nanopartikül (AgNP) sentezlenmesi ve antimikrobiyal etkinlikleri üzerine bir araştırma [An investigation on synthesis of silver-nanoparticles (AgNP) and their antimicrobial effectiveness by using herbal extracts]. Akü Femübid, 16(3), 631–641. https://doi.org/10.5578/fmbd.34220 DOI: https://doi.org/10.5578/fmbd.34220
  8. Bocquet, L., Sahpaz, S., Hilbert, J.-L., Rambaud, C., Rivière, C. (2018). Humulus lupulus L. a very popular beer ingredient and medicinal plant: overview of its phytochemistry its bioactivity and its biotechnology. Phytochem. Rev., 17(5), 1047–1090. DOI: https://doi.org/10.1007/s11101-018-9584-y
  9. Cao, C., Huang, J., Cai, W.-S., Yan, C.-N., Liu, J.-L., Jiang, Y.-D. (2017). Effects of silver nanoparticles on soil enzyme activity of different wetland plant soil systems. Soil Sed. Contam. Int. J., 26(5), 558–567. DOI: https://doi.org/10.1080/15320383.2017.1363158
  10. Chinnasamy, G., Chandrasekharan, S., Bhatnagar, S. (2019). Biosynthesis of silver nanoparticles from Melia azedarach: Enhancement of antibacterial wound healing antidiabetic and antioxidant activities. Int. J. Nanomed., 14, 9823–9836. https://doi.org/10.2147/ijn.s231340 DOI: https://doi.org/10.2147/IJN.S231340
  11. Chung, I.M., Park, I., Seung-Hyun, K., Thiruvengadam, M., Rajakumar, G. (2016). Plant-mediated synthesis of silver nanoparticles: their characteristic properties and therapeutic applications. Nanoscale Res. Lett., 11(1), 40. https://doi.org/10.1186/s11671-016-1257-4 DOI: https://doi.org/10.1186/s11671-016-1257-4
  12. Debnath, G., Das, P., Saha, A.K. (2019). Green synthesis of silver nanoparticles using mushroom extract of Pleurotus giganteus: characterization antimicrobial and α-amylase inhibitory activity. Bionanoscience, 9(3), 611–619. DOI: https://doi.org/10.1007/s12668-019-00650-y
  13. Gholamhoseinian, A., Fallah, H., Sharifi-far, F., Mirtajaddini, M. (2008). The inhibitory effect of some Iranian plants extracts on the alpha glucosidase. Iran J. Basic. Med. Sci., 11(1), 1–9. https://dx.doi.org/10.22038/ijbms.2008.5190
  14. Gul, A, Shaheen, A, Ahmad, I., Khattak, B., Ahmad, M., Ullah, R., Bari, A., Ali S.S., Alobaid, A., Asmari, M.M., Mahmood, H.M. (2021). Green synthesis characterization enzyme inhibition antimicrobial potential and cytotoxic activity of plant mediated silver nanoparticle using Ricinus communis leaf and root extracts. Biomolecules, 11(2), 206. https://doi.org/10.3390biom11020206 DOI: https://doi.org/10.3390/biom11020206
  15. Govindappa, M., Hemashekhar, B., Arthikala, M.-K., Rai, V.R., Ramachandra, Y.L. (2018). Characterization antibacterial antioxidant antidiabetic anti-inflammatory and antityrosinase activity of green synthesized silver nanoparticles using Calophyllum tomentosum leaves extract. Result. Phys., 9, 400–408. https://doi.org/10.1016/j.rinp.2018.02.049 DOI: https://doi.org/10.1016/j.rinp.2018.02.049
  16. Hosseini Bafghi, M., Safdari, H., Nazari, R., Darroudi, M., Sabouri, Z., Zargar, M., Zarrinfar, H. (2021). Evaluation and comparison of the effects of biosynthesized selenium and silver nanoparticles using plant extracts with antifungal drugs on the growth of Aspergillus and Candida species. Rendiconti Lincei Sci. Fis. Nat., 32(4), 791–803. http://dx.doi.org/10.1007/s12210-021-01021-0 DOI: https://doi.org/10.1007/s12210-021-01021-0
  17. Jini, D., Sharmila, S. (2020). Green synthesis of silver nanoparticles from Allium cepa and its in vitro antidiabetic activity. Mat. Today Proc., 22, 432–438. DOI: https://doi.org/10.1016/j.matpr.2019.07.672
  18. Khan, I., Bawazeer, S., Rauf, A., Qureshi, M.N., Muhammad, N., Al-Awthan, Y.S., Bahattab, O., Maalik, A., Rengasamy, K.R. (2022). Synthesis biological investigation and catalytic application using the alcoholic extract of Black Cumin (Bunium Persicum) seeds-based silver nanoparticles. J Nanostruct. Chem., 12, 59–77. https://doi.org/10.1007/s40097-021-00402-z DOI: https://doi.org/10.1007/s40097-021-00402-z
  19. Keskin, Ş., Saglam Ertunga, N. (2017). Purification, immobilization and characterization of thermostable α-amylase from a thermophilic bacterium Geobacillus sp. TF14. Turk. J. Biochem., 42(6), 633–642. https://doi.org/10.1515/tjb-2016-0123 DOI: https://doi.org/10.1515/tjb-2016-0123
  20. Keskin, Ş., Şirin, Y., Çakir, H.E., Keskin, M. (2019). An investigation of Humulus lupulus L.: Phenolic composition antioxidant capacity and inhibition properties of clinically important enzymes. South Afr. J. Botany, 120, 170–174. http://dx.doi.org/10.1016/j.sajb.2018.04.017 DOI: https://doi.org/10.1016/j.sajb.2018.04.017
  21. Lefahal, M., Makhloufi, E.H., Trifa, W., Ayad, R., El Hattab, M., Benahmed, M., Keskin, M., Akkal, S. (2021). The cosmetic potential of the medicinal halophyte Tamarix gallica L, (Tamaricaceae) growing in the eastern of Algeria: photoprotective and antioxidant activities. Comb. Chem. High Throughput Screen., 24(10), 1671–1678. https://doi.org/10.2174/1386207323666201204141541 DOI: https://doi.org/10.2174/1386207323666201204141541
  22. Malapermal, V., Botha, I., Krishna, S.B.N., Mbatha, J.N. (2017). Enhancing antidiabetic and antimicrobial performance of Ocimum basilicum and Ocimum sanctum (L,) using silver nanoparticles. Saudi J. Biol. Sci., 24(6), 1294–1205. https://doi.org/10.1016/j.sjbs.2015.06.026 DOI: https://doi.org/10.1016/j.sjbs.2015.06.026
  23. Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. Technol., 26(2), 211–219.
  24. Poor, M.H., Khatami, M., Azizi, H., Abazari, Y. (2017). Cytotoxic activity of biosynthesized Ag nanoparticles by Plantago major towards a human breast cancer cell line. Rendiconti Lincei, 28(4), 693–699. DOI: https://doi.org/10.1007/s12210-017-0641-z
  25. Saglam Ertunga, N., Turan, A., Akatin, M.Y., Keskin, S. (2014). Partial purification and characterization of Armillaria mellea β-glucosidase. Int. J. Food Prop., 17(3), 678–689. https://doi.org/10.1080/10942912.2012.660720 DOI: https://doi.org/10.1080/10942912.2012.660720
  26. Salari, S., Bahabadi, S.E., Samzadeh-Kermani, A., Yosefzaei, F. (2019). In-vitro evaluation of antioxidant and antibacterial potential of green synthesized silver nanoparticles using Prosopis farcta fruit extract. Iran. J. Pharm. Res. 18(1), 430–455.
  27. Singleton, V.L., Orthofer, R., Lamuela-Raventós, R.M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol., 299, 152–178. http://dx.doi.org/10.1016/S0076-6879(99)99017-1 DOI: https://doi.org/10.1016/S0076-6879(99)99017-1
  28. Singleton, V.L., Rossi, J.A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Viticult., 16(3), 144–158.
  29. Weatherburn, M.W. (1967). Phenol hypochlorite reaction for determination of ammonia. Analyt. Chem., 39(8), 971–974. https://doi.org/10.1021/ac60252a045 DOI: https://doi.org/10.1021/ac60252a045

Downloads

Download data is not yet available.

Similar Articles

<< < 2 3 4 5 6 7 8 9 10 11 > >> 

You may also start an advanced similarity search for this article.