Abstrakt
Iris species have great economic importance for their use in ornamental and pharmaceutical industry. Flora of Turkey reports about 43 local taxons of which 16 are endemic. Iris suaveolens Boiss. et Reuter is an endemic species with high seed dormancy and has high tolerance to cold and drought. This study focused on seed dormancy break of I. suaveolens under in vitro conditions. In the first experiment, the seeds were given stratification treatment on MS medium containing different concentrations of BAP with or without NAA. The 2nd experiment reports effects of alternative combinations of cold (at 4°C, 24 h dark) – warm
(16 h light photoperiod) conditions on medium containing different concentrations of plant growth regulators on agar solidified MS medium. Both experiments showed about 8.33% seed germination against 64.5% seed viability as confirmed by tetrazolium test. All germinating seeds were abnormal and very weak. In the third experiment, 10 minutes acid scarified seeds cultured at 24°C in 16 h light photo period on MS medium achieved 60.0% germination. Similar treatment under cold + dark condition was inhibitory and failed to show identical results. This underlines the importance of acid scarification, photoperiod and warm treatments of I. suaveolens seeds to break seed dormancy. All germinated seeds showed normal growth and development under field conditions.
Bibliografia
Aasim, M., Khawar, K.M., Özcan, S. (2010). Efficient in vitro propagation from preconditioned embryonic axes of Turkish cowpea (Vigna unguiculata L.) cultivar Akkiz. Arch. Biol. Sci., 62(4), 1047–1052.
Ali-Rachedi, S., Bouinot, D., Wagner, M.H., Bonnet, M., Sotta, B., Grappin, P., Jullien, M. (2004). Changes in endogenous abscisic acid levels during dormancy release and maintenance of mature seeds, studies with the Cape Verde Islands ecotype, the dormant model of Arabidopsis thaliana. Planta, 2196(1), 479–488.
Amen, R.D. (1963). The concept of seed dormancy. Am. Sci., 51, 408–424.
AOSA/SCST (2010). Tetrazolium testing handbook. Association of Official Seed Analysts and Society of Commercial Seed Technologists, Moline.
Arditti, J., Pray, T.R. (1969). Dormancy factors in Iris (Iridaceae) Seeds. Am. J. Bot., 56(3), 254–259.
Atak, A., Kaya, E., Erken, K. (2014). Determination of quantity and purity of some geophytes collected from the flora of Turkey. Asian J. Plant Sci., 13(3), 98–110.
Bell, D.T., Rokich, D.P., McChesney, C.J., Plummer, J.A. (1995). Effects of temperature, light and gibberellic acid on the germination of seeds of 43 species native to Western Australia. J. Veg. Sci., 6, 797–806.
Bethke, P.C., Libourel, I.G., Aoyama, N., Chung, Y.Y., Still, D.W., Jones, R.L. (2007). The Arabidopsis aleurone layer responds to nitric oxide, gibberellin, and abscisic acid and is sufficient and necessary for seed dormancy. Plant Physiol., 143, 1173–1188. DOI: 10.1104/pp.106.093435
Blumenthal, A., Lerner, H.R., Werker, E., Pojakoff-Mayber, A. (1986). Germination preventing mechanisms in Iris seeds. Ann. Bot., 58, 551–561.
Cone, J.W., Spruit, C.J.P. (1983). Imbibition conditions and seed dormancy of Arabidopsis thaliana. Physiol. Plant, 59, 416–420.
Coops, H., Veld, G. van der (1995). Seed dispersal, germination and seedling growth of six helophyte species in relation to water level zonation. Freshw. Biol., 34, 13–20.
Derkx, M.P.M., Karssen, C.M. (1993a). Changing sensitivity to light and nitrate but not to gibberellins regulates seasonal dormancy patterns in Sisymbrium officinale seeds. Plant Cell Environ., 166(1), 469–479.
Derkx, M.P.M., Karssen, C.M. (1993b). Variability in light-gibberellin-and nitrate requirement of Arabidopsis thaliana seeds due to harvest time and conditions of dry storage. J. Plant Physiol., 1416(1), 574–582.
Finch‐Savage, W.E., Leubner‐Metzger, G. (2006). Seed dormancy and the control of germination. New Phytol., 171(3), 501−23, https://doi.org/10.1111/j.1469-8137.2006.01787.x
Finch‐Savage, W.E., Cadman, C.S.C., Toorop, P.E., Lynn, J.R., Hilhorst, H.W.M. (2007). Seed dormancy release in Arabidopsis Cvi by dry after‐ripening, low temperature, nitrate and light shows common quantitative patterns of gene expression directed by environmentally specific sensing. Plant J., 51, 68–70, https://doi.org/10.1111/j.1365-313X.2007.03118.x
Finkelstein, R.R., Ariizumi, T., Steber, C. (2008). Molecular aspects of seed dormancy. Ann. Rev. Plant Biol., 59, 387–415.
Gomez, K.A., Gomez, A.A. (1984). Statistical procedures for agricultural research. 2nd ed. John Wiley & Sons, New York.
Graeber, K., Nakabayashi, K., Miatton, E., Leubner-Metzger, G., Soppe, W.J.J. (2012). Molecular mechanisms of seed dormancy. Plant Cell Environ., 35, 1769–1786.
Greipsson, S. (2001). Effects of stratification and GA3 on seed germination of a sand stabilising grass Leymus arenarius used in reclamation. Seed Sci. Technol., 29, 1–10.
Jones, K. (2012). Factors affecting establishment and germination of upland prairie species of conservation concern in the Willamette Valley, Oregon (Masters thesis). Corvallis, Oregon State University.
Kaššák, P. (2012). Secondary metabolites of the choosen genus Irıs species. Acta Univ. Agric. Silvic. Mendel. Brun., 60(8), 269–280.
Khawar, K.M., Cocu, S., Parmaksiz, I., Sarihan, E.O., Özcan, S. (2005). Mass proliferation of madonna lily (Lilium candidum L.) under in vitro conditions. Pak. J. Bot., 37(2), 243–248
Liu, D.F., Chen, S.H., Chen, J.W., Aotegen, Z., Yang, S.M. (1998). Reproductive characteristices, ecological and geographical distiribution of Irisi lactea var. chinensis (in Chinese). J. Inner Mongolia Inst. Agr. Animal Husbandry, 19, 1–6.
Manz, B., Müller, K., Kucera, B., Volke, F., Leubner‐Metzger, G. (2005). Water uptake and distribution in germinating tobacco seeds investigated in vivo by nuclear magnetic resonance imaging. Plant Physiol., 138, 1538–1551.
Mathew, B. (1984). Iris L. in flora of Turkey and the East Aegean Island, Davis, P.H. (ed.). Vol. 8. Edinburg Univ. Press, Edinburg, 382–410.
Mokhtarzadeh, S., Hajyzadeh, M., Ahmad, H.A., Khawar, K.M. (2013). The problems in acclimatisation of in vitro multiplied plants of Lavandula angustifolia Miller under field conditions. Acta Hortic., 988, 71–76.
Morgan, M.D. (1990). Seed germination characteristics of Iris virginica. Am. Midl. Nat., 124, 209–213.
Nadjafia, F., Bannayan, M., Tabrizi, L., Rastgoo, M. (2006). Seed germination and dormancy breaking techniques for Ferula gummosa and Teucrium polium. J. Arid. Environ., 64, 542–547.
Ozel, C.A., Khawar, K.M., Karaman, S., Ates, M.A., Arslan, O. (2008). Efficient in vitro multiplication in Ornithogalum ulophyllum Hand.-Mazz. from twin scale explants. Sci. Hortic., 116(1), 109–112.
Parmaksiz, I., Khawar, K.M. (2006). Plant regeneration by somatic embryogenesis from immature seeds of Sternbergia candida Mathew et T. Baytop, an endangered endemic plant of Turkey. Propag. Ornam. Plants, 6(3), 128–133.
Rajjou, M., Duval, K., Gallardo, J., Catusse, J., Bally, C., Job, D. (2012). Seed germination and vigor. Annu. Rev. Plant Biol., 63, 507–533.
Ritchie, S., Gilroy, S. (1998) Gibberellins, regulating genes and germination. New Phytol., 140, 363–383.
Severns, P. (2003). Inbreeding and small population size reduce seed set in a threatened and fragmented plant species, Lupinus sulphureus ssp. kincaidii (Fabaceae). Biol. Conserv., 110, 221–229.
Soyler, D., Canihoş, E., Temel, N., Hajyzadeh, M. (2013). Determi̇nati̇on of chemi̇cal fungi̇ci̇de agai̇nst soi̇l borne fungal di̇seases of capers (Cappari̇s ovata Desf. var. Herbacea) during early stages. Pak. J. Agric. Sci., 49(3), 311–314.
Soyler, D., Khawar, K.M. (2006). Effects of prechilling, scarification, incubation temperature, photoperiod, KNO3 and GA3 treatments on germination of caper (Capparis ovata Desf. var. Palaestina Zoh.) seeds. Propag. Ornam. Plants, 6(4), 159–164.
Soyler, D., Khawar, K.M. (2007). Seed germination of caper (Capparis ovata var. Herbacea) using α naphthalene acetic acid and gibberellic acid. Int. J. Agric. Biol., 9(1), 35–37.
Soyler, D., Canihos, E., Temel, N., Hajyzadeh, M. (2012). Determi̇nati̇on of chemi̇cal fungi̇ci̇de agai̇nst soi̇l borne fungal di̇seases of capers (Cappari̇s ovata Desf. var. Herbacea) during early stages. Pak. J. Agric. Sci., 49(3), 311– 314.
Stoltz, L.P. (1968). Iris seed dormancy. Physiol. Plant, 21, 1328–1331.
Sumlu, S., Atar, H.H., Khawar, K.M. (2010). Breaking seed dormancy of water lily (Nymphaea aiba L.) under in vitro conditons. Biotechnol. Biotechnol. Equipment, 24(1), 1582–1586.
Sun, Y.C., Zhang, Y.J., Wang, K., Qiu, X.J. (2006). NaOH scarification and stratification improve germination of Iris lactea var. chinensis seed. HortScience, 41, 773–774.
Tillich, H.J. (2003). Seedling morphology in Iridaceae, Indications for relationships within the family and to related families. Flora, 198, 220–242.
Tubives (2017). www.tubives.com [date of access 11.11.2017].
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