PHYSIOLOGICAL  AND  BIOCHEMICALS  CHANGES  MODULATED   BY  SEEDS’  PRIMING  OF  LENTIL  (Lens  culinaris  L.)   UNDER  SALT  STRESS  AT  GERMINATION  STAGE

Amal Bouallègue; Fatma Souissi; Issam Nouairi; Monia Souibgui; Zouhaier Abbes; Haythem Mhadhbi

doi:10.24326/asphc.2019.5.3

Tom 18 Nr 5 (2019)

Artykuły

PHYSIOLOGICAL AND BIOCHEMICALS CHANGES MODULATED BY SEEDS’ PRIMING OF LENTIL (Lens culinaris L.) UNDER SALT STRESS AT GERMINATION STAGE

Amal Bouallègue⁺⁻
Fatma Souissi⁺⁻
Issam Nouairi⁺⁻
Monia Souibgui⁺⁻
Zouhaier Abbes⁺⁻
Haythem Mhadhbi⁺⁻

PDF (English)

DOI: https://doi.org/10.24326/asphc.2019.5.3
Przesłane: 28 października 2019
Opublikowane: 2019-10-28

Abstrakt

Seed priming is one of the potential physiological approaches to enhance seed germination under salt stress. The present study examined the role of two seed priming molecules, salicylic acid (SA) and hydrogen peroxide (H₂O₂), to enhance the salt tolerance in lentil seeds at germination stage. Salt stress caused significant decrease in germination percentage and primary root elongation. This decrease was associated with significant increase in lipid peroxidation and total lipid (TL) contents in embryonic axis. The catalase (CAT), guaiacol peroxydase (GPOX) and superoxide dismutase (SOD) activities remained unchanged or decreased significantly under the influence of salt stress, in both embryonic axis and cotyledons. Starch mobilization was not affected by salt stress. The two priming treatments effectively alleviated the negative effects of salt stress. SA and H₂O₂ applications after dose optimization resulted in significant enhancement of germination percentage and primary root elongation. No significant changes in starch, soluble sugars contents and SOD activity were detected following SA and H₂O₂ treatments. Seed priming treatments triggered the activities of GPOX and CAT and caused the reduction of lipid peroxidation especially in embryonic axis. TL content and especially the fatty acid C18:3 increased after SA applications. The better performance under salt stress of primed lentil seeds was associated with lower lipid peroxidation, and activation of enzymatic antioxidative defense system. Obtained results confirm the potential for using SA and H₂O₂ to improve germination and plant growth under salt stress conditions.

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