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ASPHC Baner

Vol. 22 No. 1 (2023)

Articles

Ameliorative role of salicylic acid in the growth, nutrient content, and antioxidative responses of salt-stressed lettuce

DOI: https://doi.org/10.24326/asphc.2023.4603
Submitted: March 9, 2022
Published: 2023-02-24

Abstract

Plant responses to biotic and abiotic stresses are regulated by salicylic acid (SA), a signaling molecule. The goal of this study was to determine the efficacy of foliar SA treatments (0.25, 0.50, or 1.00 mM) in reducing salt stress in lettuce exposed to 100 mM NaCl. Salt-stressed plants given a foliar application of SA showed alleviation of the negative effects of salinity, resulting in higher growth performance (increases of 6%–198%). The positive impacts of SA were especially noticeable as an increase in the content of photosynthetic pigments, such as total chlorophyll (31–72%) and total carotenoids (49–141%). Application of SA also helped to reduce membrane damage, as seen by significantly lower levels of MDA (31–70%) in the leaves of salt-stressed lettuce plants. Moreover, the use of SA enhanced overall flavonoid and phenolic content, as well as nutrient absorption. SA treatment also increased the activities of antioxidant enzymes, such as ascorbate peroxidase, catalase, glutathione reductase, and superoxide dismutase, resulting in a considerable reduction in salt-induced oxidative damage. The most efficient SA application concentration was 0.50 mM. Overall, the use of SA as a foliar spray could be recommended as a long-term strategy for improving the defense systems of salt-stressed lettuce.

References

  1. Acosta-Motos, J.R., Ortuño, M.F., Bernal-Vicente, A., Diaz-Vivancos, P., Sanchez-Blanco, M.J., Hernandez, J.A. (2017). Plant responses to salt stress: adaptive mechanisms. Agronomy, 7(1), 1–18. https://doi.org/10.3390/agronomy7010018 DOI: https://doi.org/10.3390/agronomy7010018
  2. Arnon, D.I. (1949). Copper enzymes in isolated chloroplast: polyphenoloxidase in Beta vulgaris. Plant Physiol., 14, 1–15. https://doi.org/10.1104/pp.24.1.1 DOI: https://doi.org/10.1104/pp.24.1.1
  3. Behdad, A., Mohsenzadeh, S., Azizi, M. (2021). Growth, leaf gas exchange and physiological parameters of two Glycyrrhiza glabra L. populations subjected to salt stress condition. Rhizosphere, 17, 100319. https://doi.org/10.1016/j.rhisph.2021.100319 DOI: https://doi.org/10.1016/j.rhisph.2021.100319
  4. Bose, B., Choudhury, H., Tandon, P., Kumaria, S. (2017). Studies on secondary metabolite profiling, anti-inflammatory potential, in vitro photoprotective and skin-aging related enzyme inhibitory activities of Malaxis acuminata, a threatened orchid of nutraceutical importance. J. Photochem. Photobiol. B Biology, 173, 686–695. https://doi.org/10.1016/j.jphotobiol.2017.07.010 DOI: https://doi.org/10.1016/j.jphotobiol.2017.07.010
  5. Cakmak, I., Marschner, H. (1992). Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase in bean leaves. Plant Physiol., 98, 1222–1226. https://doi.org/10.1104/pp.98.4.1222 DOI: https://doi.org/10.1104/pp.98.4.1222
  6. Da Silva Ribeiro, J.E., Vieira de Sousa, L., Iarley da Silva, T., Silva Nóbrega, J., Andrade Figueiredo, F.R., Alcântara Bruno, R.D.L., Bandeira de Albuquerque, M. (2020). Citrullus lanatus morphophysiological responses to the combination of salicylic acid and salinity stress. Braz. J. Agric. Sci./Rev. Bras. Ciênc. Agrár., 15(1), 1–13. https://doi.org/10.5039/agraria.v15i1a6638 DOI: https://doi.org/10.5039/agraria.v15i1a6638
  7. Dasgan, H.Y., Bayram, M., Kusvuran, S., Coban, A.G., Akhoundnejad, Y. (2018). Screening of tomatoes for their resistance to salinity and drought stress. J. Biol. Agric. Health., 8(24), 31– 37.
  8. El-Taher, A.M., El-Raouf, A., Hany, S., Osman, N.A., Azoz, S.N., Omar, M.A., Mahmoud, A.M. (2022). Effect of salt stress and foliar application of salicylic acid on morphological, biochemical, anatomical, and productivity characteristics of cowpea (Vigna unguiculata L.) plants. Plants, 11(1), 1–15. https://doi.org/10.3390/plants11010115 DOI: https://doi.org/10.3390/plants11010115
  9. Ergun, O., Dasgan, H.Y., Isık, O. (2018). Effects of microalgae Chlorella vulgaris on hydroponically grown lettuce. Acta Hortic., 1273, 169–176. https://doi.org/10.17660/ActaHortic.2020.1273.23 DOI: https://doi.org/10.17660/ActaHortic.2020.1273.23
  10. Faghih, S., Ghobadi, C., Zarei, A. (2017). Response of strawberry plant cv.‘Camarosa’ to salicylic acid and methyl jasmonate application under salt stress condition. J. Plant Growth Regul., 36(3), 651–659. https://doi.org/10.1007/s00344-017-9666-x DOI: https://doi.org/10.1007/s00344-017-9666-x
  11. Gafur, M.A., Putra, E.T.S. (2019). Effect of drought stress in physiological oil palm seedling (Elaeis guineensis Jacq.) using calcium application. Asian J. Biol. Sci., 12, 550–556. DOI: https://doi.org/10.3923/ajbs.2019.550.556
  12. Ghassemi-Golezani, K., Farhadi, N. (2021). The efficacy of salicylic acid levels on photosynthetic activity, growth, and essential oil content and composition of pennyroyal plants under salt stress. J. Plant Growth Regul., 1–13. https://doi.org/10.1007/s00344-021-10515-y DOI: https://doi.org/10.1007/s00344-021-10515-y
  13. Hauser, F., Horie, T. (2010). A conserved primary salt tolerance mechanism mediated by HKT transporters:
  14. a mechanism for sodium exclusion and maintenance of high K+/Na+ ratio in leaves during salinity stress. Plant Cell Environ., 33(4), 552–565. https://doi.org/10.1111/j.1365-3040.2009.02056x DOI: https://doi.org/10.1111/j.1365-3040.2009.02056.x
  15. Heath, R.L., Packer, L. (1968). Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys., 125, 189–198. https://doi.org/10.1016/0003-9861(68)90654-1 DOI: https://doi.org/10.1016/0003-9861(68)90654-1
  16. Heidarian, F., Roshandel, P. (2021). Salicylic acid improves tolerance against salt stress through boosting antioxidant defense system in black bean. Int. J. Hortic. Sci. Technol., 8(2), 175–189. https://doi.org/10.22059/IJHST.2020.297885.345
  17. Hussein, M.M., Rezk, A.I., El-Nasharty, A.B., Mehanna, H.M. (2015). Nutritional and growth response of canola plants to salicylic acid under salt stress conditions. Int. J. ChemTech Res., 8(6), 574–581.
  18. İbrahimova, U., Kumari, P., Yadav, S., Rastogi, A., Antala, M., Suleymanova, Z., Brestic, M. (2021). Progress in understanding salt stress response in plants using biotechnological tools. J. Biotech., 329, 180–191. https://doi.org/10.1016/j.jbiotec.2021.02.007 DOI: https://doi.org/10.1016/j.jbiotec.2021.02.007
  19. Jannesar, M., Seyedi, S.M., Niknam, V., Ghadirzadeh Khorzoghi, E., Ebrahimzadeh, H. (2021). Salicylic acid, as a positive regulator of isochorismate synthase, reduces the negative effect of salt stress on Pistacia vera L. by increasing photosynthetic pigments and inducing antioxidant activity. J. Plant Growth Regul., 1–12. https://doi.org/10.1007/s00344-021-10383-6 DOI: https://doi.org/10.1007/s00344-021-10383-6
  20. Jouyban, Z. (2012). The effects of salt stress on plant growth. Tech. J. Engineer. Appl. Sci., 2(1), 7–10.
  21. Khalifa, G.S., Abdelrassoul, M., Hegazi, A.M., Elsherif, M.H. (2016). Attenuation of negative effects of saline stress in two lettuce cultivars by salicylic acid and glycine betaine. Gesunde Pflanzen, 68(4), 177–189. https://doi.org/10.1007/s10343-016-0376-2 DOI: https://doi.org/10.1007/s10343-016-0376-2
  22. Kıran, S., Kusvuran, S., Ozkay, F., Ellialtıoglu, S.S. (2019). Change in physiological and biochemical parameters under drought stress in salt-tolerant and salt-susceptible eggplant genotypes. Turk. J. Agric. For., 43, 593–602. https://doi.org/10.3906/tar-1808-1 DOI: https://doi.org/10.3906/tar-1808-1
  23. Koo, Y.M., Heo, A.Y., Choi, H.W. (2020). Salicylic acid as a safe plant protector and growth regulator. Plant Pathol. J., 36(1), 1–10. https://doi.org/10.5423/PPJ.RW.12.2019.0295 DOI: https://doi.org/10.5423/PPJ.RW.12.2019.0295
  24. Kusvuran, S. (2021). Microalgae (Chlorella vulgaris Beijerinck) alleviates drought stress of broccoli plants by improving nutrient uptake, secondary metabolites, and antioxidative defense system. Hortic. Plant J., 7(3), 221–231. DOI: https://doi.org/10.1016/j.hpj.2021.03.007
  25. Lamnai, K., Anaya, F., Fghire, R., Zine, H., Wahbi, S., Loutfi, K. (2021). Impact of exogenous application of salicylic acid on growth, water status and antioxidant enzyme activity of strawberry plants (Fragaria vesca L.) under salt stress conditions. Gesunde Pflanzen, 73(4), 465–478. DOI: https://doi.org/10.1007/s10343-021-00567-1
  26. Mohammadi, H., Hazrati, S., Janmohammadi, M. (2019). Approaches to enhance antioxidant defense in plants. In: Approaches for enhancing abiotic stress tolerance in plants, Hasanuzzaman, M., Nahar, K., Fujita, M., Oku, H., Islam, M.T. (eds). CRC Press, Taylor & Francis Group, Florida, 1–26. DOI: https://doi.org/10.1201/9781351104722-15
  27. Munawar, A., Akram, N.A., Ahmad, A., Ashraf, M. (2019). Nitric oxide regulates oxidative defense system, key metabolites and growth of broccoli (Brassica oleracea L.) plants under water limited conditions. Sci. Hortic, 254, 7–13. https://doi.org/10.1016/j.scienta.2019.04.072 DOI: https://doi.org/10.1016/j.scienta.2019.04.072
  28. Munns, R., Tester, M. (2008). Mechanisms of salinity tolerance. Ann. Rev. Plant Biol., 59, 651. https://doi.org/10.1146/annurev.arplant.59.032607.092911 DOI: https://doi.org/10.1146/annurev.arplant.59.032607.092911
  29. Poór, P., Patyi, G., Takács, Z., Szekeres, A., Bódi, N., Bagyánszki, M., Tari, I. (2019). Salicylic acid-induced ROS production by mitochondrial electron transport chain depends on the activity of mitochondrial hexokinases in tomato (Solanum lycopersicum L.). J. Plant Res., 132(2), 273–283. https://doi.org/10.1007/s10265-019-01085-y DOI: https://doi.org/10.1007/s10265-019-01085-y
  30. Poór, P. (2020). Effects of salicylic acid on the metabolism of mitochondrial reactive oxygen species in plants. Biomolecules, 10(2), 341. https://doi.org/10.3390/biom10020341 DOI: https://doi.org/10.3390/biom10020341
  31. Rajabi Dehnavi, A., Zahedi, M., Razmjoo, J., Eshghizadeh, H. (2019). Effect of exogenous application of salicylic acid on salt-stressed sorghum growth and nutrient contents. J. Plant Nutr., 42(11–12), 1333–1349. https://doi.org/10.1080/01904167.2019.1617307 DOI: https://doi.org/10.1080/01904167.2019.1617307
  32. Rehman, Z., Hussain, A., Saleem, S., Khilji, S.A., Sajid, Z.A. (2022). Exogenous application of salicylic acid enhances salt stress tolerance in lemongrass (Cymbopogon flexuosus steud. wats). Pak. J. Bot., 54(2), 371–378. https://doi.org/10.30848/PJB2022-2(13) DOI: https://doi.org/10.30848/PJB2022-2(13)
  33. Sabir, F.K., Sabir, A., Unal, S., Taytak, M., Kucukbasmaci, A., Bilgin, O.F. (2019). Postharvest quality extension of minimally processed table grapes by chitosan coating. Int. J. Fruit Sci., 19(4), 347–358. https://doi.org/10.1080/15538362.2018.1506961 DOI: https://doi.org/10.1080/15538362.2018.1506961
  34. Sarabi, B., Bolandnazar, S., Ghaderi, N., Ghashghaie, J. (2017). Genotypic differences in physiological and biochemical responses to salinity stress in melon (Cucumis melo L.) plants: prospects for selection of salt tolerant landraces. Plant Physiol. Biochem., 119, 294–311. https://doi.org/10.1016/j.plaphy.2017.09.006 DOI: https://doi.org/10.1016/j.plaphy.2017.09.006
  35. Shaki, F., Maboud, H.E., Niknam, V. (2018). Growth enhancement and salt tolerance of Safflower (Carthamus tinctorius L.), by salicylic acid. Curr. Plant Biol., 13, 16–22. https://doi.org/10.1016/j.cpb.2018.04.001 DOI: https://doi.org/10.1016/j.cpb.2018.04.001
  36. Singh, R., Upadhyay, A.K., Singh, D.P. (2018). Regulation of oxidative stress and mineral nutrient status by selenium in arsenic treated crop plant Oryza sativa. Ecotoxicol. Environ. Saf., 148, 105–113. https://doi.org/10.1016/j.ecoenv.2017.10.008 DOI: https://doi.org/10.1016/j.ecoenv.2017.10.008
  37. Türkan, I., Bor, M., Özdemir, F., Koca, H. (2005). Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Sci., 168(1), 223–231. https://doi.org/10.1016/j.plantsci.2004.07.032 DOI: https://doi.org/10.1016/j.plantsci.2004.07.032
  38. Van Aken, O., Van Breusegem, F. (2015). Licensed to kill: mitochondria, chloroplasts, and cell death. Trends Plant Sci., 20(11), 754–766. https://doi.org/10.1016/j.tplants.2015.08.002 DOI: https://doi.org/10.1016/j.tplants.2015.08.002
  39. Vázquez, J.G., Hernández-Fernández, L., Hernández, L., Pérez-Bonachea, L., Campbell, R. (2021). Physiological and biochemical response of water lettuce (Pistia stratiotes) to short-term mild saline stress. J. Plant Physiol. Pathol., 9(10), 1–6.
  40. Yang, Y., Guo, Y. (2018). Unraveling salt stress signaling in plants. J. Integr. Plant Bio., 60(9), 796–804. https://doi.org/10.1111/jipb.12689 DOI: https://doi.org/10.1111/jipb.12689

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