Abstrakt
Robinia pseudoaccacia plants grown hydroponically were treated Pb(NO3)2 with 15, 45 mg Pb2+ · dm-3. After 6, 12, 24, 72 hours of the metal treatment the plants were collected and dissected organs. The plants accumulated and transported to ground part 0.88% and 1.35% of total accumulated lead for the lower and higher dose of Pb2+ respectively. The level of GSH was differed and depended on organs, dose and time treatment of Pb2+. We investigate (different pattern of expression) expression of RpGSH1 and RpPCS genes in roots. The study showed that glutathione and genes encoded enzymes connected with synthesis of him, plays important role in the process of detoxification in plant.
Bibliografia
Akerboom T.P.M., Sies H., 1981. Assay of glutathione, glutathione disulphide, and glutathione mixed disulfides in biological samples. Meth. Enzymol. 77, 373–382.
Baycu G., Tolunay D., Özden H., Günebakan S., 2006. Ecophysiological and seasonal variations in Cd, Pb, Zn, and Ni concentrations in the leaves of urban deciduous trees in Istanbul. Environmental Pollution. 143, 545–554.
Bergmann L., Rennenberg H., 1993. Glutathione metabolism in plants. In: Sulfur Nutrittion and Asimilation in Higher Plants. 109–123.
Cunningham S.D., Ow D.W., 1996. Promises and prospects of phytoremediation. Plant Physiol. 110, 715–719.
Freeman J.L., Persans M.W., Nieman K., Albrecht C., Peer W., Pickering I.J., Slat D.E., 2004. Increased glutathione biosynthesis plays a role in nickel tolerance in thlaspi nickel hyperaccumulators. Plant Cell. 16, 2176–2179.
Mertens J., Vervaeke P., De Schrijver A., Luyssaert S., 2004. Metal uptake by young trees from dredged brackish sediment: limitations and possibilities for phytoextraction and phytostabilisation. Science of the Total Environment. 326, 209–215.
Moran J.F., Iturbe-Ormaetxe I., Matamoros M.A., Rubio M.C., Clemente M.R., Brewin N.J., Becana M., 2000. Glutathione and homoglutathione synthetases of legume nodules. Cloning, expression, and subcellular localization. Plant Physiol. 124, 1381–1392.
Oven M., Page J.E., Zenk M.H., Kutchan T.M., 2002. Molecular characterization of the homophytochelatin synthase of soybean Glycine max. Relation to phytochelatin synthase. J. Biol. Chem. 277, 4747–4754.
Piechalak A., Tomaszewska B., Baralkiewicz D., Małecka A., 2002. Accumulation and detoxification of lead in legumes. Phytochemistry 60, 153–162.
Rüegsegger A., Schmutz D., Brunold Ch., 1990. Regulation of glutathione synthesis by cadmium in Pisum sativum L. Plant Physiol. 93, 1579–1584.
Schäfer H.J., Greiner S., Rausch T., Haag-Kerwer A., 1997. In seedlings of the heavy metal accumulator Brassica juncea Cu+2 differentially affects transcript amounts for γ-glutamylcysteine synthetase (γ-ECS) and metallothionein (MT2). FEBS Lett. 404, 216–220.
Tomaszewska B., Piechalak A., 1997. Rola glutationu w detoksyfikacji ołowiu w korzeniach łubinu. Mat. Konf. Łubin we współczesnym rolnictwie. Olsztyn, 113–124.
Tomaszewska B., Tukendorf A., Barałkiewicz D., 1996. The synthesis of phytochelatins in lupin roots treated with lead ions. The Science of Legumes 3, 206–217.
Wińska-Krysiak M., 2000. Ocena przydatności na poziomie molekularnym reakcji na stres ołowiem wybranych genotypów roślin z rodziny Brassicaceae. Praca dok., SGGW, Warszawa.
Zhou J., Goldsbrough P.B., 1995. Structure, organization and expression of the metallothionein gene family in Arabidopsis. Mol. Gen. Genet. 248, 318–328.
Downloads
Download data is not yet available.
