Esra Demir

Department of Horticulture, Faculty of Agriculture, Ondokuz Mayıs University, 55200, Samsun, Turkey

Nezahat Turfan

Department of Biology, Sciences and Arts Faculty, Kastamonu University, 37200, Kastamonu, Turkey

Harun Özer

Department of Horticulture, Faculty of Agriculture, Ondokuz Mayıs University, 55200, Samsun, Turkey

Nebahat Şule Üstün

Department of Food Engineering, Faculty of Engineering, Ondokuz Mayıs University, 55200, Samsun, Turkey

Aysun Pekşen

Department of Horticulture, Faculty of Agriculture, Ondokuz Mayıs University, 55200, Samsun, Turkey


This study was carried out for the determination of nutrient and bioactive substance contents of edible wild plants consumed as vegetables in Samsun's Salıpazarı district during September 2014 and August 2016 period. In the selected villages from identified locations, 11 species that naturally grow and are consumed as vegetables such as Alcea apterocarpa Boiss., Rumex crispus L., Urtica dioica L., Trachystemon orientalis L., Oenanthe pimpinelloides L., Smilax excelsa L., Capsella bursa-pastoris L., Aegopodium podagraria L., Arum italicum Miller, Ornithogalum sigmoideum Freyn et. Sint. and Amaranthus retroflexus L. have been identified. Protein, proline, free amino acid, superoxide dismutase (SOD) activity, lipid peroxidation level (MDA), glucose, sucrose, total soluble carbohydrate, chlorophyll, total carotenoids, β-carotene, lycopene, flavonoids, and anthocyanins contents of these plants were determined as 32.79–106.40 mg/g dry weight (DW), 5.71–47.66 µmol/g DW, 29.62–61.75 µg/g DW, 82.75–240.06 IU/mg protein, 106.36–531.05 µmol/g DW, 31.96–87.24 mg/100 g DW, 10.97–25.49 mg/100 g DW, 174.3–422.2 mg/100 g DW, 7.79–25.96 mg/100 g DW, 102.01–436.93 µg/100 g DW, 115.86–459.64 µg/100 g DW, 6.38–30.28 mg/100 g DW and 10.17–21.52 mg/100 g DW, respectively. As a result of the analyses, it was determined that there were significant differences (P < 0.01) in terms of all parameters examined among species.


carotenoids, β-carotene, edible wild herbs, protein, nutritional

Aberoumand, A., Deokule, S.S. (2009). Studies on nutritional values of some wild edible plants from Iran and India. Pak. J. Nutr., 8, 26–31. DOI: 10.3923/pjn.2009.26.31

Akubugwo, I.E., Obası, A.N., Ginika, S.C. (2007). Nutritional potential of the leaves and seeds of black nightshade – Solanum nigrum L. var virginicum from Afiko-Nigeria. Pak. J. Nutr., 6, 323–326. DOI: 10.3923/pjn.2007.323.326

Arnon, D.I. (1949). Copper enzymes in isolated chloroplast. Polyphenoloxidase in Beta vulgaris. Plant Physiol., 24, 1–10. DOI: 10.1104/pp.24.1.1

Asraf, M., Foolad M.R. (2007). Roles of glycine betaine and proline in improving plant abiotic stress resistance. Env. Exper. Bot., 59, 206–216. DOI: 10.1016/j.envexpbot.2005.12.006

Baloch, F.S., Karakoy, T., Demirbas, A., Toklu, F., Ozkan, H., Hatipoglu, R. (2014). Variation of some seed mineral contents in open pollinated faba bean (Vicia faba L.) landraces from Turkey. Turk. J. Agric. For., 38, 591–601. DOI: 10.3906/tar-1311-31

Bates, L.S., Waldern, R.P., Teare, I.D. (1973). Rapid determination of free proline for water-stress studies. Plant Soil, 39, 205–207. DOI: 10.1007/BF00018060

Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utiliz-ing the principle of protein-dye binding. Analyt. Biochem., 72(1–2), 248–254. DOI: 10.1006/abio.1976.9999

Cakmak, I., Graham, R. Welch, R.M. (2002). Agricultural and molecular genetic approaches to improving nutrition and preventing Micronutrient malnutrition globally. In: Encyclopedia of Life Support Systems. Impacts of agri-culture on human health and nutrition, Cakmak, I., Welch, R.M. (eds.). Vol. 1. Unesco-Eolss Publishers, UK, 1075–1099.

Chanwitheesuk, A., Teerawutgulrag, A., Rakariyatham, N. (2005). Screening of antioxidant activity and antioxidant compounds of some edible plants of Thailand. Food Chem., 92, 491–497. DOI: 10.1016/j.foodchem.2004.07.035Get rights and content

Chu, Y.H., Chang, C.L., Hsu, H.F. (2000). Flavonoid content of several vegetables and their antioxidant activity. J. Sci. Food Agric., 80, 561–566. DOI: 10.1002/(SICI)1097-0010(200004)80:5<561::AID-JSFA574>3.0.CO;2-%23

Civelek, C. (2011). Bafra Ovası’nda sebze olarak kullanılan yabani bitkilerin toplanması, bazı besin içeriklerinin saptanması ve ıslah amaçlı olarak değerlendirilmesi. Yüksek Lisans Tezi, Ondokuz Mayıs Üniversitesi, Fen Bilimleri Enstitüsü, Samsun.

Debnath, C.S., Ricard, E. (2009). ISSR, anthocyanin content and antioxidant activity analyses to characterize strawberry genotypes. J. Appl. Hortic., 11(2), 83–89.

Dutta, P., Islam, M.N., Mondal, S. (2014). Interactive effect of arsenic stress and seed phytate content on germina-tion and seedling development of different vegetable crops. Plant Physiol. Pathol., 2, 2. DOI: 10.4172/2329-955X.1000124

Eppendorfer, W.H., Bille, S.W. (1996). Free and total amino acid composition of edible parts of beans, kale, spin-ach, cauliflower and potatoes as influenced by nitrogen fertilization and phosphorus and potassium deficiency. J. Sci. Food Agric., 71(4), 449–458. DOI: 10.1002/(SICI)1097-0010(199608)71:4<449::AID-JSFA601>3.0.CO;2-N

Ersus, S.U. (2004). Kara havuç (Daucus carota L.) antosiyanin ekstraktının püskürtmeli kurutucu kullanılarak mikroenkapsülasyonu. Doktora Tezi, Ege Üniversitesi Fen Bilimleri Enstitüsü.

Güner, A., Aslan, A., Ekim, T., Vural, M., Babaç, M.T. (2012). Türkiye Bitkileri Listesi (Damarlı Bitkiler). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği, İstanbul.

Grune, T., Lietz, G., Palou, A., Ross, A.C., Stahl, W., Tang, G., Thurnham, D., Yin, S., Biesalski, H.K. (2010). β-Carotene is an important vitamin A source for humans. J. Nutr., 140(12), 2268–2285. DOI: 10.3945/jn.109.119024

Hedge, J.E., Hofreiter, B.T. (1962). In: Carbohydrate Chemistry, 17, Whistler, R.L., Be Miller, J.N. (eds.), Academic Press, New York.

Hegazy, A.K., Al-Rowaily, S.L., Kabiel, H.F., Faisal, M., Emam, M.H. (2013). Variations of plant macronutrients and secondary metabolites content in response to radionuclides accumulation. J. Bioremed. Biodeg., 4, 185. DOI: 10.4172/2155-6199.1000185

Hodges, D.M., DeLong, J.M., Forney, C.F., Prange, R.K. (1999). Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta, 207, 604–611. DOI: 10.1007/s004250050524

Huang, Y., Bie, Z., Liu, Z., Zhen, A., Wang, W. (2009). Protective role of proline against salt stress is partially related to the improvement of water status and peroxidase enzyme activity in cucumber. Soil Sci. Plant Nutr., 55(5), 698–704. DOI: 10.1111/j.1747-0765.2009.00412.x

Jaleel, C.A. (2008). Salt stress mitigation by calcicum chloride in Phyllanhus amarus. Acta. Bot. Croat., 67(1), 53–62.

Johnson, E.J. (2002). The role of carotenoids in human health. Nutr. Clin. Care, 5(2), 56–65. DOI: 10.1046/j.1523-5408.2002.00004.x

Kaya, İ., İncekara, N. (2000). Ege Bölgesi’nde yiyecek olarak kullanılan bazı yabancı otların bileşimi. Türkiye Herboloji Dergisi, 3(2), 56–64.

Kençe, A. (1991). Biological diversity and development, 57–66. Handbook of Sustainable Development, EPFT Publications.

Kibar, B., Temel, S. (2015). Evaluation of mineral composition of some wild edible plants growing in the Eastern Anatolia Region Grasslands of Turkey and consumed as vegetable. J. Food Process. Preserv., 40, 56–66. DOI: 10.1111/jfpp.12583

Köşkeroğlu, S. (2006). Tuz ve su stresi altındaki mısır (Zea mays) bitkisinde prolin birikim düzeyleri ve stres parametrelerinin araştırılması. Yüksek Lisans Tezi, Muğla Üniversitesi Fen Bilimleri Enstitüsü, Muğla.

Kumaran, A., Karunakaran, R.J. (2006). Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus. Food Chem., 97, 109–114. DOI: 10.1016/j.foodchem.2005.03.032

Li, Q., Kubota, C. (2009). Effects of supplemental light quality on growth and phytochemicals of baby leaf lettuce. Env. Exper. Bot., 67, 59–64. DOI: 10.1016/j.envexpbot.2009.06.011

Lin, J.Y., Tang, C.Y. (2007). Determination of total phenolic and flavonoid contents in selected fruits and vegetables, as well as their stimulatory effects on Mouse splenocyte proliferation. Food Chem., 101, 140–147. DOI: 10.1016/j.foodchem.2006.01.014

Lisiewska, Z., Kmiecik, W., Korus, A. (2008). The amino acid composition of kale (Brassica oleracea L. var. acephala), fresh and after culinary and technological processing. Food Chem., 108, 642–648. DOI: 10.1016/j.foodchem.2007.11.030

Lutts, S., Kinet, J.M., Bouharmont, J. (1996). Effects of various salts and of mannitol on ion and proline accumulation in relation to osmotic adjustment in rice (Oryza sativa L.) callus cultures. J. Plant Physiol., 149, 186–195. DOI: 10.1016/S0176-1617(96)80193-3

Maisuthisakul, P., Suttajit, M., Pongsawatmanit, R. (2007). Assessment of phenolic content and free radical-scavenging capacity of some Thai indigenous plants. Food Chem., 100, 1409–1418. DOI: 10.1016/j.foodchem.2005.11.032

Moore, S., Stein, W.H. (1948). Photometric methods for use in the chromatography of amino acids. J. Biol. Chem., 176, 307–318.

Nagata, M., Yamashita, I. (1992). Simple method for simultaneous determination of chlorophyll and carotenoids in tomato fruit. Ippon Shokuhin Kogyo Gakkaishi, 39(10), 925–928. DOI: 10.3136/nskkk1962.39.925

Özgen, U., Kaya, Y., Coskun, M. (2004). Ethnobotanical studies in the villages of the district of Ilıca (Province Erzurum), Turkey. Econ. Bot., 58, 691–696. DOI: 10.1663/0013-0001(2004)058[0691:ESITVO]2.0.CO;2

Padmavati, M., Sakthivel, N., Thara, T.V., Reddy, A.R. (1997). Differential sensitivity of rice pathogens to growth inhibition by flavonoids. Phytochemistry, 46, 449–502.

Pardo, J.E., Escribano, J., Gómez, R., Alvarruiz, A. (2007). Physical-chemical and sensory quality evaluation of garlic cultivars. J. Food Qual., 30(5), 609–622. DOI: 10.1111/j.1745-4557.2007.00146.x

Pearson, D., Melon, H.K., Ronald, S. (1976). Chemical analysis of Food, 8th Edition. Churchill Livingstone, 5–63.

Ratnam, D.V., Ankola, D.D., Bhardwaj, V. (2006). Role of antioxidants in prophylaxis and therapy, A pharmaceutical perspective. J. Control. Release, 113, 189–207. DOI: 10.1016/j.jconrel.2006.04.015

Redzic, S.J. (2006). Wild edible plants and their traditional use in the human nutrition in Bosnia-Herzegovina. Ecol. Food Nutr., 45, 189–232. DOI: 10.1080/03670240600648963

Roe, M., Church, S., Pinchen, H., Finglas, P. (2013). Nutrient analysis of fruit and vegetables. Analytical Report. Institute of Food Research, Norwich Research Park, Colney, Norwich.

Sikora, E., Bodziarczyk, I. (2012). Composition and antioxidant activity of kale (Brassica oleracea L. var. acepha-la) raw and cooked. Acta Sci. Pol., Technol. Aliment., 11(3), 239–248.

Topaloğlu, K. (2010). Tuz Stresinin Chili Biberlerinin Pigment Ve Kapsaisinoid Değişimi İle Peroksidaz Aktivitesi Arasındaki İlişki. Yüksek Lisans Tezi, Çukurova Üniversitesi Fen Bilimleri Enstitüsü, Adana.

Tuna, A.L., Eroğlu, B. (2017). Effects of some organic and inorganic compounds on antioxidative system in pepper (Capsicum annuum L.) plant under salt stress. Anadolu J. Agric. Sci., 32, 121–131. DOI: 10.7161/omuanajas.289038

Turfan, N., Kurnaz, A., Alay, M., Sarıyıldız, T. (2016). Determining of some chemical properties in taşköprü garlic stored in different conditions. Kastamonu Univ. J. Forest. Fac., 16(2), 427–437.

Volk, G.M., Stern, D. (2009). Phenotypic characteristics of ten garlic cultivars grown at different North American locations. HortScience, 44, 1238–1247.

Witham, F.H., Blaydes, D.F., Devlin, R.M. (1971). Experiments in plant physiology. Van Nostrand Reinhold Company, New York, 55–56.

Yıldırım, E., Dursun, A., Turan, M. (2001). Determination of the nutrition contents of the wild plants used as vegetables in upper Çoruh Valley. Turk. J. Bot., 25, 367–371.

Yılmaz, İ. (2010). Antioksidan içeren bazı gıdalar ve oksidatif stres. İnönü Üniversitesi Tıp Fakültesi Dergisi, 17(2), 143–153.



Esra Demir 
Department of Horticulture, Faculty of Agriculture, Ondokuz Mayıs University, 55200, Samsun, Turkey
Nezahat Turfan 
Department of Biology, Sciences and Arts Faculty, Kastamonu University, 37200, Kastamonu, Turkey
Harun Özer 
Department of Horticulture, Faculty of Agriculture, Ondokuz Mayıs University, 55200, Samsun, Turkey
Nebahat Şule Üstün 
Department of Food Engineering, Faculty of Engineering, Ondokuz Mayıs University, 55200, Samsun, Turkey
Aysun Pekşen 
Department of Horticulture, Faculty of Agriculture, Ondokuz Mayıs University, 55200, Samsun, Turkey



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