THE USE OF TRANSGENIC PLANTS FOR THE DEVELOPMENT OF SELECTED BIOPRODUCTS – ACHIEVEMENTS OF THE POLISH SCIENTISTS
Magda Zapalska
University of Life Sciences in LublinKrzysztof Kowalczyk
University of Life Sciences in LublinAbstract
Green biotechnology plays an important role in the modern agriculture. Recent progress in molecular biology and genetic engineering provides an opportunity of obtaining transgenic plants with improved characteristics, such as yield parameters, nutritional value, taste, biochemical composition, cold tolerance or pathogen resistance. Genetically modified plants can be used to produce a variety of recombinant proteins of biomedical or industrial significance, including enzymes, antigens, antibodies, hormones and secondary metabolites. Research on transgenic plants has been carried out in many countries. While there has been great development of green biotechnology worldwide, some significant achievements in this field can be contributed to Polish scientists. The paper presents research on genetically modified crops carried out in Polish scientific centers and its potential usage in different areas of man life.
Keywords:
transformation, genetic engineering, GM plants, green biotechnology, recombinant proteinsReferences
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Bartoszewski G., Niedziela A., Szwacka M., Niemirowicz-Szczytt K., 2003. Modification of tomato taste in transgenic plants carrying a thaumatin gene from Thaumatococcus daniellii Benth. Plant Breeding 122(4), 347–351.
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Czubacka A., Doroszewska T., 2010. Estimating agronomic traits of transgenic tobacco lines. Euphytica 172, 35–47.
Fedorowicz O., Bartoszewski G., Kamińska M., Stoeva P., Niemirowicz-Szczytt K., 2005. Pathogen-derived resistance to Tomato spotted wilt virus in transgenic tomato and tobacco plants. J. Amer. Soc. Hort. Sci. 130(2), 218–224.
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Gandikota M., Kochk, A., Chen L., Ithal1 N., Fauquet C., Reddy1 A.R., 2001. Development of transgenic rice plants expressing maize anthocyanin genes and increased blast resistance. Mol. Breed. 7, 73–83.
Gasparis S., Orczyk W., Zalewski W., Nadolska-Orczyk A., 2011. The RNA-mediated silencing of one of the Pin genes in allohexaploid wheat simultaneously decreases the expression of the other, and increases grain hardness. J. Exp. Bot. 62(11), 4025–4036.
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Kapusta J., Modelska A., Figlerowicz M., Pniewski T., Letellier M., Lisowa O., Yusibov V., Koprowski H., Plucienniczak A., Legocki A.B., 1999. A plant-derived edible vaccine against hepatitis B virus. FASEB J. 13, 1796–1799.
Kapusta J., Pniewski T., Wojciechowicz J., Bociąg P., Płucienniczak A., 2010. Nanogram doses of alum-adjuvanted HBs antigen induce humoral immune response in mice when orally administered. Arch. Immunol. Ther. Exp. 58, 143–151.
Karpiński S., Szechyńska-Hebda M., Wituszyńska W., Burdiak P., 2012. Light acclimation, retrograde signalling, cell death and immune defences in plants. Plant Cell Environ. DOI: 10.1111/pce.12018.
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Kowalczyk K., Gruszecka D., Nowak M., Leśniowska-Nowak J., 2011. Resistance of Triticale Hybrids with Pm4b and Pm6 Genes to Powdery Mildew. Acta Biologica Cracoviensia, Series Botanica, 53(1), 57–62.
Kowalczyk K., Okoń S., Leśniowska-Nowak J., Nowak M., 2009. Wykorzystanie genów odporności na rdzę brunatną Lr19, Lr21, Lr35 w programach hodowlanych pszenicy zwyczajnej w Polsce. Zesz. Probl. Post. Nauk Roln. 542, 255–260.
Lorenc-Kukuła K., Amarowicz R., Oszmiański J., Doermann P., Starzycki M., Skała J., Żuk M., Kulma A., Szopa J., 2005a. Pleiotropic effect of phenolic compounds content increases in transgenic flax plant. J. Agric. Food Chem. 53, 3685–3692.
Lorenc-Kukuła K., Jafra S., Oszmiański J., Szopa J., 2005b. Ectopic expression of anthocyanin 5-O-glucosyltransferase in potato tuber causes increased resistance to bacteria. J. Agric. Food Chem. 53, 272–281.
Lorenc-Kukuła K., Żuk M., Kulma A., Czemplik M., Kostyn K., Skala J., Starzycki M., Szopa J., 2009. Engineering flax with the GT family 1 Solanum sogarandinum glycosyltransferase SsGT1 confers increased resistance to Fusarium infection. J. Agric. Food Chem. 57, 6698–6705.
Łukaszewicz M., Matysiak-Kata I., Skała J., Fecka I., Cisowski W., Szopa J., 2004. Antioxidant capacity manipulation in transgenic potato tuber by changes in phenolic com pounds content. J. Agric. Food Chem. 52, 1526–1533.
Masuda T., Kitabatake N., 2006. Developments in biotechnological production of sweet proteins. J. Biosci. Bioeng. 102(5), 375–389.
Musialak M., Wróbel-Kwiatkowska M., Kulma A., Starzycka E., Szopa J., 2008. Improving retting of fibre through genetic modification of flax to express pectinases. Transgenic Res. 17, 133–147.
Navabi A., Singh R.P., Tewari J.P., Briggs K.G., 2004. Inheritance of high levels of adult-plant resistance to stripe rust in five spring wheat genotypes. Crop Sci. 44, 1156–1162.
Niggeweg R., Michael A.J., Martin C., 2004. Engineering plants with increased levels of the antioxidant chlorogenic acid. Nat. Biotechnol. 22, 746–754.
Pham N.B., Schäfer H., Wink M., 2012. Production and secretion of recombinant thaumatin in tobacco hairy root cultures. Biotechnol. J. 7(4), 537–545.
Pniewski T., Kapusta J., Bociąg P., Kostrzak A., Fedorowicz-Strońska O., Czyż M., Gdula M., Krajewski P., Wolko B., Płucienniczak A., 2012. Plant expression, lyophilisation and storage of HBV medium and large surface antigens for a prototype oral vaccine formulation. Plant Cell. Rep. 31, 585–595.
Pniewski T., Kapusta J., Bociąg P., Wojciechowicz J., Kostrzak A., Gdula M., Fedorowicz-Strońska O., Wójcik P., Otta H., Samardakiewicz S., Wolko B., Płucienniczak A., 2011. Lowdose oral immunization with lyophilized tissue of herbicide-resistant lettuce expressing hepatitis B surface antigen for prototype plant-derived vaccine tablet formulation. J. Appl. Genetics 52, 125–136.
Rajam M.V., Chandola N., Saiprasad Goud P., Singh D., Kashyap V., Choudhary M.L., Sihachakr D., 2007. Thaumatin gene confers resistance to fungal pathogens as well as tolerance to abiotic stresses in transgenic tobacco plants. Biol. Plant. 51(1), 135–141.
Redkiewicz P., Więsyk A., Góra-Sochacka A., Sirko A., 2012. Transgenic tobacco plants as production platform for biologically active human interleukin 2 and its fusion with proteinase inhibitors. Plant Biotechnol. J. 10, 806–814.
Rendi-Wagner P., Shouval D., Genton B., Lurie Y., Rümke H., Boland G., Cerny A., Heimh M., Bachi D., Schroeder M., Kollaritsch H., 2006. Comparative immunogenicity of a PreS/S hepatitis B vaccine in non- and low responders to conventional vaccine. Vaccine 24, 2781–2789.
Rojas-Anaya E., Loza-Rubio E., Olivera-Flores M.T., Gomez-Lim M., 2009. Expression of rabies virus G protein in carrots (Daucus carota). Trans. Res. 18, 911–919.
Schestibratov K.A., Dolgov S.V., 2005. Transgenic strawberry plants expressing a thaumatin II gene demonstrate enhanced resistance to Botrytis cinerea. Sci. Hort. 106, 177–189.
Skórkowska-Telichowska K., Żuk M., Kulma A., Bugajska-Prusak A., Ratajczak K., Gąsiorowski K., Kostyn K., Szopa J., 2010. New dressing materials derived from transgenic flax products to treat long-standing venous ulcers – a pilot study. Wound Rep. Reg. 18, 168–179.
Szechyńska-Hebda M., Kruk J., Górecka M., Karpińska B., Karpiński S., 2010. Evidence for light wavelength-specific photoelectrophysiological signaling and memory of excess light episodes in Arabidopsis. The Plant Cell 22, 2201–2218.
Szopa J., Wróbel-Kwiatkowska M., Kulma A., Żuk M., Skórkowska-Telichowska K., Dymińska L., Mączka M., Hanuza J., Zebrowski J., Preisner M., 2009. Chemical composition and molecular structure of fibers from transgenic flax producing polyhydroxybutyrate, and mechanical properties and platelet aggregation of composite materials containing these fibers. Compos. Sci. Technol. 69, 2438–2446.
Szwacka M., Burza W., Zawirska-Wojtasiak R., Gośliński M., Twardowska A., Gajc-Wolska J., Kosieradzka I., Kiełkiewicz M., 2012. Genetically modified crops expressing 35S-thaumatin II transgene: sensory properties and food safety aspects. Compr. Rev. Food Sci. Food Safety 11, 174–186.
Szwacka M., Krzymowska M., Osuch A., Kowalczyk M.E., Malepszy S., 2002. Variable properties of transgenic cucumber plants containing the thaumatin II gene from Thaumatococcus daniellii. Acta Physiol. Plant. 24(2), 173–185
Tacket C.O., Mason H.S., Losonsky G., Estes M.K., Levine M.M., Arntzen C.J., 2000. Human Immune Responses to a Novel Norwalk Virus Vaccine Delivered in Transgenic Potatoes. J. Infect. Dis. 182, 302–305.
Thanavala Y., Mahoney M., Pal S., Scott A., Richter L., Natarajan N., Goodwin P., Arntzen C.J., Mason H.S., 2005. Immunogenicity in humans of an edible vaccine for hepatitis B. Proc. Natl. Acad. Sci. USA 102(9), 3378–3382.
Treutter D., 2005. Significance of flavonoids in plant resistance and enhancement of their biosynthesis. Plant Biology 7(6), 581–591.
Witty M., 1990. Preprothaumatin II is processed to biological activity in Solanum tuberosum. Biotechnol. Lett.12(2), 131–136.
Wróbel-Kwiatkowska M., Lorenc-Kukuła K., Starzycki M., Oszmiański J., Kepczyńska E., Szopa J., 2004. Expression of β-1,3-glucanase in flax causes increased resistance to fungi. Physiol. Mol. Plant P. 65, 245–256.
Wróbel-Kwiatkowska M., Skórkowska-Telichowska K., Dymińska L., Mączka M., Hanuza J., Szopa J., 2009. Biochemical, mechanical, and spectroscopic analyses of genetically engineered flax fibers producing bioplastic (poly-β-hydroxybutyrate). Biotechnol. Prog. 25(5), 1489–1498.
Wróbel-Kwiatkowska M., Starzycki M., Zebrowski J., Oszmiański J., Szopa J., 2007a. Lignin deficiency in transgenic flax resulted in plants with improved mechanical properties. J. Biotech. 128, 919–934.
Wróbel-Kwiatkowska M., Zebrowski J., Starzycki M., Oszmiański J., Szopa J., 2007b. Engineering of PHB synthesis caused improved elastic properties of flax fibres. Biotech. Prog. 23, 269–277.
Yin Z., Rorat T., Szabala B.M., Ziółkowska A., Malepszy S., 2006. Expression of a Solanum sogarandinum SK3-type dehydrin enhances cold tolerance in transgenic cucumber seedlings. Plant Sci. 170, 1164–1172.
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Magda Zapalska
University of Life Sciences in Lublin
University of Life Sciences in Lublin
Krzysztof Kowalczyk
University of Life Sciences in Lublin
University of Life Sciences in Lublin
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