In vitro POLLEN VIABILITY, GERMINATION AND POLLEN TUBE GROWTH IN SOME POMEGRANATE (Punica granatum L.) CULTIVARS FROM CROATIA AND BOSNIA AND HERZEGOVINA
Jelena Gadže
University of Zagreb, CroatiaMira Radunić
Institute for Adriatic Crops and Karst Reclamation Split, CroatiaIvana Vladimira Petric
Croatian Centre for Agriculture, Zagreb, CroatiaSezai Ercisli
Ataturk University, Erzurum, TurkeyAbstract
In this study in vitro pollen viability and germination in intact pollens and pollen tube growth in germinated pollens of five common pomegranate cultivars grown in
Croatia and Bosnia and Herzegovina was investigated first time. Pollen viability varied from 36.73% (cv. Konjski Zub) to 51.80% (cv. Barski) in fluorescent diacetat (FDA) test. The average pollen germination percentages were found as the lowest 6.83% in cv. Konjski Zub and the highest 42.51% in cv Glavas. Among the germination media in general 0.2 agar + 10% sucrose+5 ppm H3BO3 gave better results to obtain higher pollen germination for all cultivars. In germinated pollens of these five cultivars, significant differences were observed on pollen tube length. Average pollen tube lengths in germinated pollens of five cultivars were measured as minimum 302 μm in cv. Konjski Zub and as maximum 344 μm in cv. Ciparski. The results showed that there were obvious differences in pollen
germinability among cultivars growing under the same environmental conditions and these differences must be considered for orchard establishment to obtain higher yield.
Keywords:
pomegranate, Punica granatum, cultivars, pollen germination, pollen tube growthReferences
Beyhan N., Serdar S., 2009. In vitro pollen germination and the tube growth of some European chestnut genotypes (Castanea sativa Mill.) Fruits, 64, 157–165.
Beyhan N., Serdar U., Balik H.I., 2009. Pollen viability and germination rates of some hybrid and European chestnut pollens. Acta Hort. 815, 107–114
Cheung, A.Y., 1996. Pollen-pistil interactions during pollen tube growth. Trends Plant Sci., 1, 45–51.
Derin K., Eti S., 2001. Determination of pollen quality, quantity and effect of cross pollination on the fruit set and quality in the pomegranate. Tr. J. Agric. Forest. 25, 169–173.
Engin H., Hepaksoy S., 2003. Determination of pollen germination of some pomegranate cultivars. J. Aegean Univ. 40, 9–16.
Ercisli S., 2007. Determination of pollen viability and in vitro pollen germination of Rosa dumalis and Rosa villosa. Bangladesh J. Botany, 36, 185–187.
Ercisli S., Agar, G., Orhan E., Yildirim N., Hizarci Y., 2007. Interspecific variability of RAPD and fatty acid composition of some pomegranate cultivars (Punica granatum L.) growing in Southern Anatolia Region in Turkey. Biochem Syst. Ecol. 35, 764–769.
Gozlekci S., Kaynak L., 2000. Investigations on pollen production and quality in some standards pomegranate (Punica granatum L.) cultivars. Options Mediterraneennes, 42, 71–78.
Ilgin M., Ergenoglu F., Caglar S., 2007. Viability, germination and amount of pollen in selected caprifig types. Pak. J. Botany, 39, 9–14.
Malgarejo P., Martinez J.J., Hernandez F., 2000. A study of different culture media for pomegranate (Punica granatum L.) pollen. Options Mediterraneennes, 42, 63–69.
Onur C., 2000. Pomegranate (Special volume). Derim, 5, 1–68.
Sari-Gorla M., Pe ME., Rossini L., 1994. Detection of QTLs controlling pollen germination and growth in maize. Heredity, 72, 332–335.
Sharafi Y, Babash Pour M., Karimi M., 2010. In vitro pollen germination and pollen tube growth in some Rosa canina genotypes. Int. Med. Arom. Plants, 29–31.
Stosser R., Hartman W., Anvari S.F., 1996. General aspects of pollination and fertilization of pomes and stone fruits. Acta Hort, 423, 15–21.
Taylor P., 1997. Pollen germination and tube growth. Annu. Rev. Plant Physiol. Plant Mol. Biol., 48, 461–491.
Tosun F., Koyuncu F., 2007. The effects of some chemicals on pollen germination and pollen tube development in sweet cherry (Prunus avium L.). J. Akdeniz Univ. Agric. Fac. 20, 219–224.
Wang Q., Lu L., Wu, X., Li, Y., Lin, J., 2003. Boron influences pollen germination and pollen tube growth in Picea meyeri. Tree Physiol. 23, 345–351
Yilmaz C., 2009. Pomegranate, Hasat Publisher, 76 pp.
University of Zagreb, Croatia
Institute for Adriatic Crops and Karst Reclamation Split, Croatia
Croatian Centre for Agriculture, Zagreb, Croatia
Ataturk University, Erzurum, Turkey
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