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Vol. 15 No. 5 (2016)

Articles

In vitro PROPOGATION OF Physalis peruviana (L.) USING APICAL SHOOT EXPLANTS

Submitted: October 29, 2020
Published: 2016-10-31

Abstract

Physalis peruviana L. is belongs to Solanaceae family and commonly known as Cape gooseberry. More recently it is very popular and widely used as medicinal plant to treat malaria, asthma, hepatitis, dermatitis and rheumatism and has diuretic and antiinflammatory properties. In this study, it was aimed to develop in vitro propagation protocol for P. peruviana L. using apical shoots as an explant sources. Regenerated plants were evaluated based on their multiplication rate and shoot length using various concentration of BAP (1, 2, 3 mg l-1) in combination with IBA (0, 0.1, 0.2, 0.4 mg l-1) and NAA (0, 0.1, 0.2, 0.4 mg l-1). In addition, efficiency of various auxin concentrations of (1 and 2 mg l-1 IBA and NAA) was also applied on root formation of P. peruviana L. The highest shoot numbers were obtained from 2 mg l-1 BAP with 0.4 mg l-1 IBA (6.00) combinations and shoot length obtained in 2 mg l-1 BAP with 0.2 mg l-1 IBA combinations (3.30 cm). As for the effects of BAP and NAA combinations; the highest shoot length were obtained from 2 mg l-1 BAP without NAA combinations (3.33 cm) while the lowest one was in 3 mg l-1 BAP with 0.4 mg/I NAA combinations. The highest root numbers were obtained from NAA application (2 mg l-1 and 1 mg l-1, respectively). In vitro derived plants were acclimatized to the soil smoothly. The present study highlights the importance of plant tissue culture in order to be used for large-scale production of P. peruviana (L.) due to the elimination of sexual propagation.

References

Afroz, F., Hassan, S.Y., Bari, L.S., Sultana, R.K., Begum, N., Akter, M.A., Khatun, J. (2009). In vitro shoot proliferation and plant regeneration of Physalis minima L. – a perennial medicinal herb. Bangladesh J. Sci. Ind. Res., 44(4), 453–456.
Bajpai, P.K., Warghat, A.R., Sharma, R.K., Yadav, A., Thakur, A.K., Srivastava, R.B., Stobdan, T. (2014). Structure and genetic diversity of natural populations of Morus alba in the Trans-Himalayan Ladakh Region. Biochem. Genet., 52, 137–152.
Baksha, R., Jahan, M.A.A., Khatun, R., Munshi, J.L. (2007). In vitro rapid clonal propagation of Rauvolfia serpentina (Linn.) Benth. Bangladesh J. Ind. Res., 42(1), 37–44.
Boulay, M. (1987). In vitro propagation of tree species. Plant Tissue and Cell Culture, New York, 367–381.
Boxus, P., Damiano, C., Brasseur, E. (1989). Strawberry. In: Handbook of plant cell culture. Sharp, W.R., Evans, D.A., Ammirato, P.V., Yamada, Y. (eds). Ammirato, Evans, Sharp and Yamada, Mecmillan NY, 453–486.
Cakir, O., Pekmez, M., Cepni, E., Candar, B., Fidan, K. (2014). Evaluation of biological activities of Physalis peruviana ethanol extracts and expression of Bcl-2 genes in HeLa cells. Food Sci. Technol., 34(2), 422–430.
Chalupa, V. (1987). Clonal propagation of broad-leaved forest trees. In vitro Commun. Inst For. Chech., 12, 255–257
Cao, X., Hammerschlag, F.A. (2000). Improved shoot organogenesis from leaf explants of highbush blueberry. HortSci., 35, 945–947.
Feng, S.G., Lu, J.J., Gao, L., Liu, J.J., Wang, H.Z. (2014). Molecular phylogeny analysis and species identification of Dendrobium (Orchidaceae) in China. Biochem. Genet., 52, 127–136.
Hassan, A.K.M.S., Roy, S.K. (2005). Micropropagation of Gloriosa superba L. through high frequency shoot proliferation. Plant Tissue Cult. Biotechnol., 15(1), 67–74.
Lloyd, G., McCown, B.H. (1981). Commercially-feasible micropropagation of Mountain laurel, Kalmia latifolia, by shoot tip culture. Proc. Int. Plant. Prop. Soc., 30, 421–427.
Mlcek, J., Valsikova, M., Druzbikova, H., Ryant, P., Jurikova, T., Sochor, J., Borkovcova, M. (2015). The antioxidant capacity and macroelement content of several onion cultivars. Turk. J. Agric. For., 39, 999–1004.
Mungole, A.J., Vilas, D., Doifode, R.B., Kamble, A., Chaturvedi Zanwar P. (2011). In vitro callus induction and shoot regeneration in Physalis minima L. Ann. Biol. Res., 2(2), 79–85.
Oscar, M.M., Solano, P.M., Zapata, E.V., Castro, O.C., González-Arnao, M.T., Guevara-Valencia, M., Luna-González, A., Díaz-Ramos, C. (2011). Enlargement and rooting of peruviana cherry (Physalis peruviana L.) in vitro plants. Trop. Subtropic. Agroecosyst., 13, 537–542.
Otroshy, M., Arash, M., Sayyed, M.M.K., Amir-Hossein, B. (2013). Direct regeneration from leaves and nodes explants of Physalis peruviana L. Int. J. Farm All. Sci., 2(9), 214–218.
Pardo, J.M., Fontanilla, M.R., Ospina, L.F., Espinosa, L. (2008). Determining the pharmacological activity of Physalis peruviana fruit juice on rabbit eyes and fibroblast primary cultures. Invest Ophthalmol. Vis. Sci., 49(7), 3074–3079.
Perk, B.O., Ilgin, S., Atli, O., Duymus, H.G., Sirmagul, B. (2013). Acute and Subchronic toxic effects of the fruits of Physalis peruviana L. Evid. Based Complement. Alternat. Med., 707285, 1–10.
Rahman, S.M.R., Afroz, F., Sultana, K., Sen, P.K., Ali, M.R. (2006). Effect of growth regulators and state of medium on micropropagation of Adhatoda vasica (Nees.) Khulna University Studies, Special Issue (Proceedings of 1st Research Cell Conference), 55–59.
Ramirez, F., Fischer, G., Davenport, T.L., Augusto Pinzon, J.C., Ulrichs, C. (2013). Cape gooseberry (Physalis peruviana L.) phenology according to the BBCH phonological scale. Sci. Hortic., 162, 39–42.
Ruttanapraset, R., Banterng, P., Jogloy, S., Vorasoot, N., Kesmala, T., Kanwar, R.S., Holbrook, C.C., Patanothai, A. (2014). Genotypic variability for tuber yield, biomass, and drought tolerance in Jerusalem artichoke germplasm. Turk. J. Agric. For., 38, 570–580.
Sahoo, Y., Chan, P.K. (1998). Micropropagation of Vitex negundo L., a woody aromatic medicinal plant shrub, through high frequency axillary shoot proliferation. Plant Cell Rep., 18, 301–307.
Taji, A., Kumar, P.P., Lakshmanan, P. (2002). In vitro plant breeding. New York: Food Products Press.
Usha, P.K., Benjamin, S., Mohanan, K.V. (2007). An efficient micropropagation system for Vitex negundo L., an important woody aromatic medicinal plant, through shoot tip culture. Res. J. Bot., 2, 102–107.
Vadawale, A.V., Barve, D.M., Dave, A.M. (2006). In vitro flowering and rapid propagation of Vitex negundo L.-a medicinal plant. Indian J. Biotechnol., 5, 112–116.
Wu, S.J., Ng, L.T., Chen, C.H., Lin, D.L., Wang, S.S., Lin, C.C. (2004). Antihepatoma activity of Physalis angulata and P. peruviana extracts and their effects on apoptosis in human Hep G2 cells. Life Sci., 74(16), 2061–2073.
Wu, S.J., Tsai, J.Y., Chang, S.P., Lin, D.L., Wang, S.S., Huang, S.N. (2006). Supercritical carbon dioxide extract exhibits enhanced antioxidant and anti-inflammatory activities of Physalis peruviana. J. Ethnopharmacol., 108(3), 407–413.
Yen, C.Y., Chiu, C.C., Chang, F.R., Chen, T., Hwang, C.C., Hseu, Y.C., Yang, H.L., Alan, Y.L., Ming, T.T., Guo, Z.G., Cheng, Y.S. (2010). 4b-Hydroxywithanolide E from Physalis peruviana (golden berry) inhibits growth of human lung cancer cells through DNA damage, apoptosis and G2/M arrest. BMC Cancer, 10, 46.
Yücesan, B.B., Mohammed, A., Arslan, M., Gürel E. (2015). Clonal propagation and synthetic seed production from nodal segments of Cape gooseberry (Physalis peruviana L.), a tropical fruit plant. Turk. J. Agric. For., 39(5), 797–806.
Zimmermann, R.H. (1981). Micropropagation of fruit plants. Growth Regulators in fruit production. Acta Hortic., 120, 217–227.

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