THE EFFECT OF GROWTH RETARDANTS APPLIED IN VITRO ON THE ACCLIMATIZATION AND GROWTH OF Tibouchina urvilleana cogn. IN VIVO
Danuta Kozak
Agricultural University in LublinAbstract
Shoot tips of Tibouchina urvilleana Cogn. were cultured 4 weeks in vitro in modified Murashige and Skoog (MS) [1962] medium supplemented with growth retardants: paclobutrazol – 0.1, 0.5, 1.0, 5.0 mg.dm-3, flurprimidol – 0.1, 1.0, 5.0 mg.dm-3, chlorocholine chloride (CCC) – 5.0, 50.0, 250.0 mg.dm-3. Explants cultured on the medium without growth substances were used as a control. Rooted microcuttings were transferred to the greenhouse and transplanted into a mixture of 1 peat : 1 perlite, where they were grown for 5 weeks. Plants were then cultivated in a peat substrate during another 5 weeks. Acclimatization of rooted shoots in the greenhouse was affective in 92.5–100%. The survival of plants was lowest when microcuttings were previously cultured on medium with flurprimidol at 5.0 mg.dm-3. Cultivation of Tibouchina urvilleana shoots in vitro on media with various growth retardants had a significant effect on the further growth of plants ex vitro. Paclobutrazol and flurprimidol at 5.0 mg.dm-3 inhibited very strong growth of plants after 10 weeks of growth ex vitro.
Keywords:
Tibouchina urvilleana, growth retardants, in vitro, acclimatization, in vivoReferences
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Agricultural University in Lublin
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