THE EFFECT OF CHITOSAN ON THE FORMATION OF MICROORGANISM COMMUNITIES IN THE RHIZOSPHERE SOIL OF SOYBEAN
Alina Pastucha
Agricultural University in LublinAbstract
The experiment was conducted in the years 1999–2001. The object of the studies was the rhizosphere soil of soybean ‘Polan’ cv., and 0.1% chitosan. This compound was used once (seed dressing), twice (seed dressing and seedling spraying), three times (seed dressing, seedling spraying and plant spraying at anthesis). The experiment also considered a control combination, i.e. without chitosan. The purpose of the studies was to determine the effect of chitosan on the qualitative and quantitative composition of microorganisms in rhizosphere soil with antagonists distinguished.
The microbiological analysis of samples of soybean rhizosphere soil taken from particular experimental combinations pointed to different numbers of bacteria and fungi. The greatest total number of bacteria colonies and the smallest number of fungi colonies were characteristic of the rhizosphere soil of soybean after using chitosan three times. This sample of soil contained the greatest number of bacteria and fungi with antagonistic effect towards plant pathogens.
Keywords:
chitosan, soybean, rhizosphere, antagonistic bacteria, antagonistic fungiReferences
Badura L., 2004. Czy znamy wszystkie uwarunkowania funkcji mikroorganizmów w ekosystemach lądowych. Probl. Nauk Biol. Kosmos 53, ¾, 373–379.
Funck-Jensen D., Hockenhull J., 1984. Root exudation, rhizosphere microorganisms and disease control. Växtskyddsnotiser 48, 49–54.
Mańka K., 1974. Zbiorowiska grzybów jako kryterium oceny wpływu środowiska na choroby roślin. Zesz. Probl. Post. Nauk Rol. 160, 9–23.
Martin J. P., 1950. Use of acid, rose bengal and streptomycin in the method for estimating soil fungi. Soil Sci. 38, 215–220.
Martyniuk S., Masiak D., Stachyra W., Myśków., 1991. Populations of microorganisms of the root zone of various grasses and their antagonisms towards Gaeumannomyces graminis var. tritici. Pam. Puł. Pr. IUNG 98, 139–144.
Myśków W., 1989. Związki między aktywnością biologiczną gleby, a jej żyznością i urodzajnocią. Mat. Szkol. Biologiczne metody podnoszenia żyzności i urodzajności gleb. Puławy, 51–53.
Orlikowski L. B., Skrzypczak C., Niekraszewicz A., Struszczyk H., 2001. Influence of chitosan on the development of Fusarium wilt of carnation. Progres on Chemistry and Application of Chitin and Its Derivatives, Struszczyk H. ed. VII, 155–158, Polish Chitin Soc. Łódź 2001.
Parke J. L., 1990. Root colonization by indigenous and introduced microorganisms. [in:] The Rhizosphere and Plant Growth. D. L. Keister P. B. Gregan (eds.), Kluwer Academic Publishers, Durdrecht, The Netherlands, 33–42.
Patkowska E., 2001. Bacterial and fungal populations in the rhizosphere of various plants as related to root exudates. J. Plant Protect. Res. 41, 3, 240–249.
Pięta D., 1988. Mikozy występujące w uprawach fasoli (Phaseolus vulgaris L.) i podatność różnych odmian na porażenie przez niektóre grzyby. Ser. Wyd. – Rozpr. Nauk. AR Lublin.
Pięta D., 1999. Initial studies of populations of fungi bacteria in the soil under influence of the cultivation of spring wheat and winter wheat in a growth chamber. Acta Agrobot. 52, 1–2, 161-166.
Pięta D., Pastucha A., Struszczyk H., 2000. Skuteczność zaprawiania chitozanem nasion fasoli wielokwiatowej (Phaseolus coccineus L.) przez grzyby. Rocz. AR. Poznań, CCCXXIII, Ogrodn. 31, 1, 409–413.
Pięta D., Pastucha A., Struszczyk H., Wójcik W. 2001. Kształtowanie się zbiorowisk mikroorganizmów w glebie pod wpływem chitozanu i uprawy fasoli wielokwiatowej (Phaseolus coccineus L.) Acta Agrobot. 54, 2, 105–115.
Pospieszny H., 1977. Niektóre aspekty stosowania chitozanu w ochronie roślin. Prog. Plant Protect. 37, (1), 306-309.
Pospieszny H., Struszczyk H., 1994. Chitozan – potencjalny biopreparat przeciwko patogenom roślin. Mat. XXXIV Sesji Nauk. IOR, cz. I. Poznań, 117–124.
Rovira A. D., 1969. Plant root exudates. Bot. Rev. 35, 35–57.
Teichgraber P., Popper L., Knorr D., 1991. Chitosan as elicitor for the production of chitynase an antifungal enzyme from soybean seeds. Agro Industry Hi. Tech. 2, 3, 11–14
Transmo A., Skaugrud O., Harman G. E., 1993. Chitosan induces resistance in crop plants their fungal pathogens. Phytopathology 46, 4, 411–414.
Agricultural University in Lublin
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