Evaluation of capsicum genotypes for resistance to cucumber mosaic virus
Ilyas Deligoz
Department of Plant Health, Black Sea Agricultural Research Institute, Samsun, Turkiyehttps://orcid.org/0000-0003-3646-300X
Abstract
Cucumber mosaic virus (CMV) is one of the most destructive viruses that affect pepper all over the world. Using resistant varieties is one of the most effective ways to control the virus. Identification of new resistance sources is essential for the development of resistant cultivars. In the present study, the reactions of 50 Capsicum genotypes (25 Capsicum baccatum, 15 C. annuum and 10 C. frutescens) were determined against CMV-Sa isolate with mechanical inoculation under controlled conditions in 2020. A 0–4 disease rating scale was used to analyze the genotypes, and the genotypes were categorized as resistant, moderately resistant and susceptible based on disease incidence and disease severity scores. The findings revealed that there were significant (p < 0.01) differences in disease incidence and severity among genotypes. The study demonstrated that one of the 25 tested C. baccatum genotypes (KTB-11) found to be resistant, and three genotypes (KTB-29, KTB-34, KTB-57 and KTB-72) were rated as moderately resistant to CMV. However, it was determined that all tested C. frutescens and C. annuum genotypes were susceptible to CMV. The genotypes identified as resistant and moderately resistant can be used as a source of resistance in pepper breeding studies.
Keywords:
CMV, screening, C. frutescens, C. annuum, C. baccatumReferences
Ali, A., Kobayashi, M. (2010). Seed transmission of Cucumber mosaic virus in pepper. J. Virol. Methods, 163(2), 234–237. https://doi.org/10.1016/j.jviromet.2009.09.026 DOI: https://doi.org/10.1016/j.jviromet.2009.09.026
Balcı, E. (2005). Genetic characterization of Cucumber mosaic virus (CMV) resistance in tomato and pepper [master thesis]. İzmir Institute of Technology, 55 p.
Başay, S., Uzunoğulları, N. (2016). F1 Hibrit dolma biber ıslahında Cucumber mosaic virus, Tobacco mosaic virus ve Potato virus Y virus testlemeleri [Screening of Cucumber mosaic virus Tobacco mosaic virus and Potato virus Y on F1 hybrid bell pepper breeding]. Alatarım, 15(2), 37–43.
Caranta, C., Pflieger, S., Lefebvre, V., Daubeze, A., Thabuis, A., Palloix, A. (2002). QTLs involved in the restriction of Cucumber mosaic virus (CMV) long-distance movement in pepper. Theor. Appl. Genet., 104(4), 586–591. https://doi.org/10.1007/s001220100753 DOI: https://doi.org/10.1007/s001220100753
Castagnoli, F., Polverari, A., Marte, M. (1997). Behaviour of an accession of Capsicum baccatum var. pendulum towards Cucumber mosaic virus. Phytopathol. Mediterr., 36(3), 154–158.
Chaim, A.B., Grube, R., Lapidot, M., Jahn, M., Paran, I. (2001). Identification of quantitative trait loci associated with resistance to Cucumber mosaic virus in Capsicum annuum. Theor. Appl. Genet., 102(8), 1213–1220. https://doi.org/10.1007/s001220100581 DOI: https://doi.org/10.1007/s001220100581
Clark, M.F., Adams, A.N. (1977). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol., 34(3), 475–483. https://doi.org/10.1099/0022-1317-34-3-475 DOI: https://doi.org/10.1099/0022-1317-34-3-475
FAO. (2020). Food and Agriculture Organization of the United Nations. Available from: http://www.fao.org/faostat/en/#data/QV Accessed 16.06.2020
Grube, R.C., Zhang, Y., Murphy, J.F., Loaiza-Figueroa, F., Lackney, V.K., Provvidenti, R., Jahn, M. (2000). New source of resistance to Cucumber mosaic virus in Capsicum frutescens. Plant Dis., 84(8), 885–891. https://doi.org/10.1094/PDIS.2000.84.8.885 DOI: https://doi.org/10.1094/PDIS.2000.84.8.885
Jacquemond, M. (2012). Cucumber mosaic virus. Adv. Virus Res., 84, 439–504. https://doi.org/10.1016/B978-0-12-394314-9.00013-0 DOI: https://doi.org/10.1016/B978-0-12-394314-9.00013-0
Kang, W.H., Hoang, N.H., Yang, H.B., Kwon, J.K., Jo, S.H., Seo, J.K., Kim, K.H., Choi, D., Kang, B.C. (2010). Molecular mapping and characterization of a single dominant gene controlling CMV resistance in peppers (Capsicum annuum L.). Theor. Appl. Genet., 120(8), 1587–1596. https://doi.org/10.1007/s00122-010-1278-9 DOI: https://doi.org/10.1007/s00122-010-1278-9
Kenyon, L., Kumar, S., Tsai, W.S., Hughes, J.D.A. (2014). Virus diseases of peppers (Capsicum spp.) and their control. Adv. Virus Res., 90, 297–354. https://doi.org/10.1016/B978-0-12-801246-8.00006-8 DOI: https://doi.org/10.1016/B978-0-12-801246-8.00006-8
Keleş Öztürk, P., Baloğlu, S. (2019). Doğu Akdeniz Bölgesi’nde açık alanda yetiştirilen biberlerde bazı virüslerin serolojik ve moleküler tanısı [Serological and molecular detection of some viruses in open-field peppers in the eastern mediterranean region]. Alatarım, 18(1), 1–11.
Li, N., Yu, C., Yin, Y., Gao, S., Wang, F., Jiao, C., Yao, M. (2020). Pepper crop improvement against Cucumber mosaic virus (CMV): a review. Front. Plant Sci., 11, 598–798. https://doi.org/10.3389/fpls.2020.598798 DOI: https://doi.org/10.3389/fpls.2020.598798
Lapidot, M., Paran, I., Be-Joseph, R., Ben-Harush, S., Pilowsky, M., Cohen, S., Shifriss, C. (1997). Tolerance to Cucumber mosaic virus in pepper: development of advanced breeding lines and evaluation of virus level. Plant Dis., 81(2), 185–188. https://doi.org/10.1094/PDIS.1997.81.2.185 DOI: https://doi.org/10.1094/PDIS.1997.81.2.185
Manzur, J.P., Fita, A., Prohens, J., Rodríguez-Burruezo, A. (2015). Successful wide hybridization and introgression breeding in a diverse set of common peppers (Capsicum annuum) using different cultivated Ají (C. baccatum) accessions as donor parents. Plos One, 10(12), e0144142. https://doi.org/10.1371/journal.pone.0144142 DOI: https://doi.org/10.1371/journal.pone.0144142
Marco, S., Cohen, S. (1979). Rapid detection and titer evaluation of viruses in pepper by enzyme-linked immunosorbent assay. Phytopathology, 69, 1259–1262. https://doi.org/10.1094/Phyto-69-1259 DOI: https://doi.org/10.1094/Phyto-69-1259
Monma, S., Sakata, Y. (1997). Screening of Capsicum accessions for resistance to Cucumber mosaic virus. J. Jap. Soc. Hort. Sci., 65(4), 769–776. https://doi.org/10.2503/jjshs.65.769 DOI: https://doi.org/10.2503/jjshs.65.769
Moury, B., Verdin, E. (2012). Viruses of pepper crops in the Mediterranean basin: a remarkable stasis. Adv. Virus Res., 84, 127–162. https://doi.org/10.1016/B978-0-12-394314-9.00004-X DOI: https://doi.org/10.1016/B978-0-12-394314-9.00004-X
Naresh, P., Reddy, M.K., Reddy, P.H.C., Reddy, K.M. (2016). Screening chilli (Capsicum spp.) germplasm against Cucumber mosaic virus and Chilli veinal mottle virus and inheritance of resistance. Eur. J. Plant Pathol., 146(3), 451–464. https://doi.org/10.1007/s10658-016-0930-x DOI: https://doi.org/10.1007/s10658-016-0930-x
Parisi, M., Daniela, A., Pasquale, T. (2020). Overview of biotic stresses in pepper (Capsicum spp.): sources of genetic resistance, molecular breeding and genomics. Int. J. Mol. Sci., 21(7), 2587. https://doi.org/10.3390/ijms21072587 DOI: https://doi.org/10.3390/ijms21072587
Palukaitis, P., Roossinck, M.J., Dietzgen, R.G., Francki, R.I.B. (1992). Cucumber mosaic virus. Adv. Virus Res., 41, 281–349. https://doi.org/10.1016/S0065-3527(08)60039-1 DOI: https://doi.org/10.1016/S0065-3527(08)60039-1
Pernezny, K., Robert, P.D., Murphy, J.F., Goldberg, N.P. (2003). Compendium of pepper diseases. The American Phytopatholgical Society, St. Paul, MN, 1, 24–25
Rahman, M.S., Akanda, A.M., Mian, I.H., Bhuiyan, M.K.A., Hossain, M.M. (2016). New sources of resistance to Cucumber mosaic virus in Capsicum annuum. J. Crop. Sci. Biotechnol., 19(3), 249–258. https://doi.org/10.1007/s12892-016-0044-1 DOI: https://doi.org/10.1007/s12892-016-0044-1
Scott, A.J., Knott, M. (1974). A cluster analysis method for grouping means in the analysis of variance. Biometrics, 30(3), 507–512. https://doi.org/10.2307/2529204 DOI: https://doi.org/10.2307/2529204
Suzuki, K., Kuroda, T., Miura, Y., Murai, J. (2003). Screening and field trials of virus resistant sources in Capsicum spp. Plant Dis., 87(7), 779–783. https://doi.org/10.1094/PDIS.2003.87.7.779 DOI: https://doi.org/10.1094/PDIS.2003.87.7.779
TUIK. (2021). Turkish Statistical Institute. Plant Production Statistics. www.tuik.gov.tr
Uzunoğulları, N., Gümüş, M. (2015). Marmara bölgesinde bazı kültür bitkilerinde doğal enfeksiyona neden olan hyar mozaik virüsü (Cucumber mosaic virus, CMV)’ nün tespiti [Detection of Cucumber mosaic virus causing natural infection on some cultured plants in Marmara Region]. Trakya Univ. J. Nat. Sci., 16(1), 9–15.
Waweru, B.W., Kilalo, D.C., Kimenju, J.W., Rukundo, P., Miano, D.W. (2020). Evaluation of hot pepper (Capsicum spp.) genotypes for resistance to viruses and aphids in Rwanda. Adv. Hortic. Sci., 34(4), 397–412. https://doi.org/10.13128/ahsc8094
Yao, M., Li N., Wang F., Ye, Z. (2013). Genetic analysis and identification of QTLs for resistance to Cucumber mosaic virus in chili pepper (Capsicum annuum L.). Euphytica, 193(2), 135–145. https://doi.org/10.1007/s10681-013-0953-8 DOI: https://doi.org/10.1007/s10681-013-0953-8
Yoon, J.B., Yang, D.C., Do, J.W., Park, H.G. (2006). Overcoming two post-fertilization genetic barriers in interspecific hybridization between Capsicum annuum and C. baccatum for introgression of anthracnose resistance. Breed. Sci., 56 (1), 31–38. https://doi.org/10.1270/jsbbs.56.31 DOI: https://doi.org/10.1270/jsbbs.56.31
Department of Plant Health, Black Sea Agricultural Research Institute, Samsun, Turkiye https://orcid.org/0000-0003-3646-300X
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