INOCULATION WITH N2-FIXING PLANT GROWTH PROMOTING RHIZOBACTERIA TO REDUCE NITROGEN FERTILIZER REQUIREMENT OF LETTUCE
Haluk Çağlar Kaymak
Department of Horticulture, Faculty of Agriculture, Atatürk University, 25240 Erzurum, TurkeyAdem Aksoy
Department of Agricultural Economy, Faculty of Agriculture, Atatürk University, 25240 Erzurum,Recep Kotan
Department of Plant Protection, Faculty of Agriculture, Atatürk University, 25240 Erzurum, TurkeyAbstract
This study was undertaken to determine the effect of three different mixtures of some N2-fixing plant growth-promoting rhizobacteria (PGPR) on growth, yield, element content and nitrate accumulation as well as the effect on the reduction of nitrogen fertilization of lettuce (Lactuca sativa L.). The measurements were made in two separate experiments in 2015 between 6 June – 5 August (Experiment 1) and 2 July – 3 September (Experiment 2) under the field conditions. Butterhead form and heat tolerant summer cultivar ‘Luna’ was used as a plant material. Agrobacterium rubi RK-34, Pantoea agglomerans RK-79 and RK-92, Pseudomonas putida RK-142 and TV-42A, Bacillus megaterium TV-6D, TV-60D and TV-91C, Pseudomonas flourescens TV-11D and Paenibacillus polymyxa TV-12E were used as N2-fixing plant growth promoting rhizobacteria. The treatments were 150 kg N/ha (available dose of AS) as ammonium sulphate (AS) [(NH4)2SO4),
(21% N)] and three different mixtures of PGPR. Further, combined uses of decreasing doses of AS
(50%, 75 kg ha–1AS and 75%, 112.5 kg ha–1 AS) and PGPR mixtures (M) such as M-1 + 75 AS, M-1 +
112.5 AS, M-2 + 75 AS, M-2 + 112.5 AS, M-3 + 75 AS and M-3 + 112.5 AS were used as additional treatments. All treatments increased the yield and the growth of lettuce according to the control. While inoculation with PGPR mixtures decreased the accumulation of heavy metals such as Cd, Ni, and Pb in lettuce, increased nutrient uptake of lettuce. It was determined that the nitrate accumulation of lettuce (cv. ‘Luna’) in PGPR mixtures were lower than the available dose of AS but higher than control. The yield in M-3 + 112.5 AS (48431 kg ha–1) was similar and in the same statistical group with the available dose of AS (48225 kg ha–1) in both experiments. Furthermore, according to the results of cost analyses, using 25% less of AS (112.5 kg ha–1) with M-3 will supply the same income instead of using AS (150 kg ha–1). It can be clearly said that the mixtures with some N2-fixing plant growth promoting rhizobacteria (PGPR), especially M-3 (P. putida
RK-142 + P. flourescens TV-11D + B. megaterium TV-91C), have a great potential to decrease the nitrogen use (25%) for environmentally friendly crop production of lettuce.
Keywords:
biofertilizer, benefit/cost ratio, Lactuca sativa L., net profi, rhizobacteria, yieldReferences
Abramovic, H., Abram, V., Cuk, A., Ceh, B., Smole-Mozina, S., Vidmar, M., Pavlovic, M., Ulrih, N.P. (2018). Antioxidative and antibacterial properties of organically grown thyme (Thymus sp.) and basil (Ocimum basilicum L.). Turk. J. Agric. For., 42, 185–194. DOI: 10.3906/tar-1711-82
Bashan, Y., Holguin, G., De-Bashan, L.E. (2004). Azospirillum-plant relationships: physiological, molecular, agricultural, and environmental advances (1997–2003). Can. J. Microbiol., 50, 521–577. DOI: 10.1139/w04-035
Bremner, J.M. (1996). Nitrogen-total. The soil science society of America and the American society of agronomy. Madison, Wisconsin, 1085–1121.
Chabot, R., Antoun, H., Cescas, M.P. (1996). Growth promotion of maize and lettuce by phosphate-solubilizing Rhizobium leguminosarum biovar. phaseoli. Plant Soil, 184, 311–321. DOI: 10.1007/BF00010460
Chamangasht, S., Ardakani, M.R., Khavazi, K., Abbaszadeh, B., Mafakheri, S. (2012). Improving lettuce (Lactuca sativa L.) growth and yield by the application of biofertilizers. Ann. Biol. Res., 3, 1876–1879.
Çakmakçı, R., Erat, M., Erdoğan, Ü., Dönmez, M.F. (2007). The influence of plant growth-promoting rhizobacteria on growth and enzyme activities in wheat and spinach plants. J. Plant. Nutr. Soil. Sci., 170, 288–295. DOI: 10.1002/jpln.200625105
Decoteau, R.D. (2000). Lettuce. In: Vegetable crops, Linsner, K. (ed.), 238–252.
Flores‐Félix, J.D., Menéndez, E., Rivera, L.P., Marcos‐García, M., Martínez‐Hidalgo, P., Mateos, P.F., Rivas, R. (2013). Use of Rhizobium leguminosarum as a potential biofertilizer for Lactuca sativa and Daucus carota crops. J. Plant Nutr. Soil Sci., 176, 876–882. DOI: 10.1002/jpln.201300116
Gasoni, L., Cozzi, J., Kobayashi, K., Yossen, V., Zumelzu, G., Babbitt, S., Kahn, N. (2001). Yield response of lettuce and potato to bacterial and fungal inoculants under field conditions in Córdoba (Argentina). Z. Pflanzenkr. Pflanzenschutz., 108, 530–535.
Güvenç, İ., Karataş, A., Kaymak, H.Ç. (2006). Effects of foliar applications of urea, ethanol and pudrecine on growth and yield of lettuce (Lactuca sativa). Indian J. Agric. Sci., 76, 23–25.
Jeffrey, C. (2007). Compositae. Introduction with key to tribes. In: The families and genera of vascular plants. Vol. 8: Flowering plants. Eudicots. Asterales, Kadereit, J.W., Jeffrey, C. (eds.). Springer, 61–87.
Kaymak, H.Ç. (2010). Potential of PGPR in agricultural innovations. In: Plant growth and health promoting bacteria, Maheshwari, D.K. (ed.). Springer, 45–79.
Kaymak, H.Ç., Dönmez, M.F., Çakmakçi, R. (2013). N2-fixing plant growth promoting rhizobacteria: As a potential application to increase yield, growth and element contents of leaves in Mentha piperita L. Eur. J. Plant Sci. Biotechnol., 7 (Special Issue 1: “Vegetable Science and Biotechnology in Turkey”), 38–42.
Klement, Z., Farkas, G.L., Lovrekovich, L. (1964). Hypersensitive reaction induced by phytopathogenic bacteria in the tobacco leaf. Phytopathology, 54, 474–477.
Kloepper, J.W., Schroth, M.N. (1978). Plant growth-promoting rhizobacteria on radishes. In: Proceedings of the Fourth International Conference on Plant Pathogen Bacteria, INRA, 879–882.
Kohler, J., Caravaca, F., Carrasco, L. Rolda, N.A. (2006). Contribution of Pseudomonas mendocina and Glomus intraradices to aggregate stabilization and promotion of biological fertility in rhizosphere soil of lettuce plants under field conditions. Soil Use Manage, 22, 298–304. DOI: 10.1111/j.1475-2743.2006.00041.x
Kokalis-Burelle, N., Vavrina, E.N., Rosskopf, E.N., Shelby, R.A. (2002). Field evaluation of plant growth-promoting rhizobacteria amended transplant mixes and soil solarization for tomato and pepper production in Florida. Plant Soil, 238, 257–266. DOI: 10.1023/A:1014464716261
Korkmaz, K., Ibrikci, H., Ryan, J., Buyuk, G., Guzel, N., Karnez, E., Oguz, H., Yagbasanlar, T. (2008). Optimizing nitrogen fertilizer-use recommendations for winter wheat in a mediterranean-type environment using tissue nitrate testing. Commun. Soil Sci. Plant Anal., 39, 1352–1366. DOI: 10.1080/00103620802004052
Lai, W.A., Rekha, P.D., Arun, A.B., Young, C.C. (2008). Effect of mineral fertilizer, pig manure, and Azospirillum rugosum on growth and nutrientcontents of Lactuca sativa L. Biol. Fertil. Soils, 45, 155–164. DOI: 10.1007/s00374-008-0313-3.
Mantelin, S., Touraine, B. (2004). Plant growth-promoting bacteria and nitrate availability: impacts on root development and nitrate uptake. J. Exp. Bot., 55, 27–34. DOI: 10.1093/jxb/erh010
Maroniche, G.A., Rubio, E.J., Consiglio, A., Perticari, A. (2016). Plant-associated fluorescent Pseudomonas from red lateritic soil: Beneficial characteristics and their impact on lettuce growth. J. Gen. Appl. Microbiol., 62, 248–257. DOI: 10.2323/jgam.2016.04.006
Mertens, D. (2005a). AOAC official method 922.02. Plants preparation of laboratuary sample. Official methods of analysis, Gaitherburg, 1–2.
Mertens, D. (2005b). AOAC Official method 975.03. Metal in plants and pet foods. Official methods of analysis, Gaitherburg, 3–4.
Mohite, B. (2013). Isolation and characterization of indole cetic acid (IAA) producing bacteria from rhizospheric soil and its effects on plant growth. J. Soil. Sci. Plant Nutr., 13, 638–649. DOI: 10.4067/S0718-95162013005000051
Naveed, M., Khalid, M., Jones, D.L., Ahmad, R., Zahir, Z.A. (2008). Relative efficacy of Pseudomonas spp., containing ACC-Deaminase for improving growth and yield of maize (Zea mays L.) in the presence of organic fertilizer. Pak. J. Bot., 40, 1243–1251.
Orhan, E., Esitken, A., Ercisli, S., Turan, M., Sahin, F. (2006). Effects of plant growth promoting rhizobacteria (PGPR) on yield, growth and nutrient contents in organically growing raspberry. Sci. Hortic., 111, 38–43. DOI: 10.1016/j.scienta.2006.09.002
Ruzzi, M., Aroca, R. (2015). Plant growth-promoting rhizobacteria act as biostimulants in horticulture. Sci. Hortic., 196, 124–134. DOI: 10.1016/j.scienta.2015.08.042
Shantharam, S., Mattoo, A.K. (1997). Enhancing biological nitrogen fixation: An appraisal of current and alternative technologies for N input into plants. Plant Soil, 194, 205–216. DOI: 10.1023/A:1004234315999
Şahin, F., Çakmakçi, R., Kantar, F. (2004). Sugar beet and barley yields in relation to inoculation with N2-fixing and phosphate solubilizing bacteria. Plant Soil, 265, 123–129. DOI: 10.1007/s11104-005-0334-8
Vessey, J.K. (2003). Plant growth promoting rhizobacteria as biofertilizers. Plant Soil, 255, 571–586. DOI: 10.1023/A:1026037216893
Department of Horticulture, Faculty of Agriculture, Atatürk University, 25240 Erzurum, Turkey
Department of Agricultural Economy, Faculty of Agriculture, Atatürk University, 25240 Erzurum,
Department of Plant Protection, Faculty of Agriculture, Atatürk University, 25240 Erzurum, Turkey
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