Metabolomic analysis of Chinese yam (Dioscorea polystachya Turczaninow) bulbils at different germination stages by UPLC-Q-TOF-MS
Xiaojin Ge
Institute of Chinese Herbel Medicines, Henan Academy of Agricultural Sciences; Henan Provincial Center for Engineering and Technology Research of Traditional Chinese Medicine, Zhengzhou, Henan 450046, P.R. Chinahttps://orcid.org/0009-0007-7510-6116
Xiangyang Li
Institute of Chinese Herbel Medicines, Henan Academy of Agricultural Sciences; Henan Provincial Center for Engineering and Technology Research of Traditional Chinese Medicine, Zhengzhou, Henan 450046, P.R. Chinahttps://orcid.org/0009-0006-6376-3453
Dandan Dai
Institute of Chinese Herbel Medicines, Henan Academy of Agricultural Sciences; Henan Provincial Center for Engineering and Technology Research of Traditional Chinese Medicine, Zhengzhou, Henan 450046, P.R. Chinahttps://orcid.org/0009-0000-5593-6330
Zhen Yang
Xixian Agricultural Science Research Institutehttps://orcid.org/0009-0005-4250-9299
Yanhong Wang
Institute of Chinese Herbel Medicines, Henan Academy of Agricultural Sciences; Henan Provincial Center for Engineering and Technology Research of Traditional Chinese Medicine, Zhengzhou, Henan 450046, P.R. Chinahttps://orcid.org/0009-0001-0157-6242
Tiegang Yang
Institute of Chinese Herbel Medicines, Henan Academy of Agricultural Sciences; Henan Provincial Center for Engineering and Technology Research of Traditional Chinese Medicine, Zhengzhou, Henan 450046, P.R. Chinahttps://orcid.org/0000-0003-0119-3417
Guixiao La
Institute of Chinese Herbel Medicines, Henan Academy of Agricultural Sciences; Henan Provincial Center for Engineering and Technology Research of Traditional Chinese Medicine, Zhengzhou, Henan 450046, P.R. Chinahttps://orcid.org/0009-0001-7542-4532
Abstract
Bulbil germination is crucial to the survival of Chinese yam plants, the preservation of germplasm resources and the worldwide supply of food and natural medicine. There are still some unknowns regarding bulbil biochemical variations associated with germination. The metabolic changes during the germination of Chinese yam (Dioscorea polystachya Turczaninow) bulbils were studied using ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) at eight-time points covering all four phases of germination. It was determined that 27 metabolites, including organic acids, amino acids, sugars, lipid metabolites, phenolics and steroids, were responsible for the variation in the Chinese yam bulbil groups. A metabolomics pathway was proposed based on the identified metabolites. The main processes affected during germination were those related to carbohydrate metabolism, the TCA cycle, lipid metabolism, nitrogen metabolism, lipid metabolism and polyphenol metabolism. It is one of the earliest reports on the metabolite identification and profiling of Chinese yam bulbils at different germination stages.
Keywords:
Chinese yam bulbils, germination, metabolomics, UPLC-Q-TOF-MS, multivariate statistical analysis, metabolism pathwayReferences
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Institute of Chinese Herbel Medicines, Henan Academy of Agricultural Sciences; Henan Provincial Center for Engineering and Technology Research of Traditional Chinese Medicine, Zhengzhou, Henan 450046, P.R. China https://orcid.org/0009-0007-7510-6116
Institute of Chinese Herbel Medicines, Henan Academy of Agricultural Sciences; Henan Provincial Center for Engineering and Technology Research of Traditional Chinese Medicine, Zhengzhou, Henan 450046, P.R. China https://orcid.org/0009-0006-6376-3453
Institute of Chinese Herbel Medicines, Henan Academy of Agricultural Sciences; Henan Provincial Center for Engineering and Technology Research of Traditional Chinese Medicine, Zhengzhou, Henan 450046, P.R. China https://orcid.org/0009-0000-5593-6330
Institute of Chinese Herbel Medicines, Henan Academy of Agricultural Sciences; Henan Provincial Center for Engineering and Technology Research of Traditional Chinese Medicine, Zhengzhou, Henan 450046, P.R. China https://orcid.org/0009-0001-0157-6242
Institute of Chinese Herbel Medicines, Henan Academy of Agricultural Sciences; Henan Provincial Center for Engineering and Technology Research of Traditional Chinese Medicine, Zhengzhou, Henan 450046, P.R. China https://orcid.org/0000-0003-0119-3417
Institute of Chinese Herbel Medicines, Henan Academy of Agricultural Sciences; Henan Provincial Center for Engineering and Technology Research of Traditional Chinese Medicine, Zhengzhou, Henan 450046, P.R. China https://orcid.org/0009-0001-7542-4532
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