DO JASMONIC ACID AND ACTIVATED CHARCOAL INCREASE THE in vitro DEVELOPMENT OF ORANGE CARROT (Daucus carota L.) AND PURPLE CARROT (Daucus carota L. ssp. sativus var. atrorubens Alef.)?

Tugce OZSAN

Department of Horticulture, Faculty of Agriculture, Akdeniz University, Antalya, Turkey
https://orcid.org/0000-0002-3265-6886

Elif Gulsun VURAL

Department of Horticulture, Faculty of Agriculture, Akdeniz University, Antalya, Turkey

Ahmet Naci ONUS

Department of Horticulture, Faculty of Agriculture, Akdeniz University, Antalya, Turkey
https://orcid.org/0000-0001-8615-1480


Abstract

Present study aimed to reveal the effects of jasmonic acid and activated charcoal on in vitro carrot plantlet regeneration by using Murashige and Skoog (MS) medium supplemented with BAP, NAA, activated charcoal and jasmonic acid at various concentrations. To serve the purpose, in vitro carrot seed germination, shoots, cotyledons, and first leaves formation of orange and purple carrot plantlets were investigated. During the experiments, root size, weight, and size of petiole diameter, hyperhydricity and callus formation rate were recorded. Experimental results revealed that combination of jasmonic acid and activated charcoal in medium had a positive effect especially on the first stage of developmental processes such as seed swelling and germination, cotyledon and first leaf formation as well as having positive effects on above-ground internode elongation, petiole and plantlet height.

Keywords:

carrot, early plant growth, jasmonates, plant tissue culture

Ahmadi, B., Shariatpanahi, M.E., da Silva, J.A.T. (2014). Efficient induction of microspore embryogenesis using abscisic acid, jasmonic acid and salicylic acid in Brassica napus L. Plant Cell Tiss. Organ Cult., 116, 343–351. DOI: 10.1007/s11240-013-0408-x

Baktır, İ. (2015). Jasmonateler (Yasemin Asitleri). Hormonlar, Bitki Gelişim Düzenleyicileri, Özellikleri ve Tarımda Kullanımları [Jasmonates (Jasmine Acids). Hormones, Plant Growth Regulators, Properties and Uses in Agriculture]. İstanbul, Türkiye: Hasad Yayıncılık, sf. 90–92 [in Turkish].

Berger, S., Bell, E., Mullet, J.E. (1996). Two methyl jasmonate-insensitive mutants show altered expression of AtVsp in response to methyl jasmonate and wounding. Plant Physiol., 111, 525–531. DOI: 10.1104/pp.111.2.525

Creelman, R.A., Mullet, J.E. (1997). Biosynthesis and action jasmonates in plants. Ann. Rev. Plant Physiol. Plant Mol. Biol., 48, 355–381.

Çavusoglu, K., Kabar, K. (2006). Does Jasmonic Acid Prevent The Germination of Barley Seeds? SDÜ Fen Edebiyat Fakültesi Fen Dergisi (E-Dergi), 1(12–2), 35–41.

Çavusoglu, K., Kabar, K., Kılıç, S. (2007). Effects of Some Plant Growth Regulators on Jasmonic Acid Induced Inhibition of Seed Germination and seedling Growth of Barley. SDÜ Fen Edebiyat Fakültesi. Fen Dergisi (E-Dergi), 2(1), 53–59.

Dereli, U. (2010). Siyah Havuç Suyu Konsantresi Üretimi ve Depolanması Sürecinde Fenolik Maddelerdeki Değişimler ve Bu Değişimlerin Antioksidan Aktivite ile İlişkisi. Yüksek Lisans Tezi. Ankara Üniversitesi, Fen Bilimleri Enstitüsü.

Gaspar, T., Kevers, C., Penel, C., Greppin, H., Reid, D.M., Thorpe, T.A. (1996). Plant Hormones and Plant Growth Regulators In Plant Tissue Culture. In vitro Cell. Dev. Biol.-Plant, 32, 272–289.

Grebenstein, C., Kos, S.P., de Jong, T.J., Tamis, W.L.M., de Snoo, G.R. (2013). Morphological markers for the detection of introgression from cultivated into wild carrot (Daucus carota L.) reveal dominant domestication traits. Plant Biol., 15, 531–540.

Hanci, F., Cebeci, E. (2012). Siyah Havuç (Daucus carota ssp. sativus var. atrorubens Alef.) Yetiştiriciliği. Tarım Türk Dergisi, 7, 36–40 [in Turkish].

Jourdan, M., Gagné, S., Dubois-Laurent, C., Maghraoui, M., Huet, S., Suel, A., Hamama, L., Briard, M., Peltier, D., Geoffriau, E. (2015). Carotenoid content and root color of cultivated carrot: a candidate-gene association study using an original broad unstructured population. PLoS One, 10:e0116674, 1–19. DOI: 10.1371/journal.pone.0116674

Kenar, S. (2013). Patateste (Solanum tuberosum L.) in vitro Mikrotuberizasyon Üzerine Jasmonik Asit – Giberellik Asit Etkileşiminin Araştırılması. Yüksek Lisans Tezi, Hacettepe Üniversitesi, Ankara, Turkey [in Turkish].

Kepczynski, J., Bialecka, B., Kepczynska, E. (1999). Ethylene biosynthesis in Amaranthus caudatus seeds in response to methyl jasmonate. J. Plant Growth Regul., 28, 59–65. DOI: 10.1023/A:1006201625637

Koda, Y., Kikuta, Y., Takazi, H., Tsujino, Y., Sakamura, S., Yoshihara, T. (1991). Potato tuber-inducing activities of jasmonic acid and related compounds. Phytochemistry, 30(5), 1435–1438. DOI: 10.1016/0031-9422(91)84180-Z

Koda, Y., Kikuta, Y. (1991). Possible involvement of jasmonic acid in tuberization in yam plants. Plant Cell Physiol., 32, 629–633.

Koda, Y., Kikuta, Y. (2001). Effects of jasmonates on in vitro tuberization in several potato cultivars that differ greatly in maturity. Plant Prod. Sci., 4(1), 66–70. DOI: 10.1626/pps.4.66

Lorenzo, O., Solano, R. (2005). Molecular players regulating the jasmonate signalling network. Curr. Opin. Plant Biol., 8, 532–540. DOI: 10.1016/j.pbi.2005.07.003.

Luby, C.H., Maeda, H.A., Goldman, I.L. (2014). Genetic and phenological variation of tocochromanol (vitamin E) content in wild (Daucus carota L. var. carota) and domesticated carrot (D. carota L. var. sativa). Hortic. Res., 1(15), 1–6. DOI: 10.1038/hortres.2014

Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15, 473–97.

Nowacka, M., Wedzik, M. (2016). Effect of ultrasound treatment onmicrostructure, colour and carotenoid content in fresh and dried carrot tissue. Appl. Acoust., 103, 163–171. DOI: 10.1016/j.apacoust.2015.06.011

Pelacho, A.M., Mingo-Castel, A.M. (1991). Jasmonic acid induces tuberization of potato stolons cultured in vitro. Plant Physiol., 97, 1253–1255. DOI: 10.1104/pp.97.3.1253

Parthier, B. (1991). Jasmonates, new regulators of plant growth and development: Many facts and few hypotheses of their action. Bot. Acta, 104, 446–454. DOI: 10.1111/j.1438-8677.1991.tb00257.x

Rathinapriya, P., Satish, L., Rameshkumar, R., Pandian, S., Rency, A.S., Ramesh, M. (2019). Role of activated charcoal and amino acids in developing an efficient regeneration system for foxtail millet (Setaria italica (L.) Beauv.) using leaf base segments. Physiol. Mol. Biol. Plants, 25(2), 533–548. DOI: 10.1007/s12298-018-0619-z

Ravnikar, M., Gogala, N. (1990). Regulation of potato meristem development by jasmonic acid in vitro. J. Plant Growth Regul., 9, 233–236. DOI: 10.1007/BF02041968

Ravnikar, M., Vilhar, B., Gogala, N. (1992). Stimulatory effects of jasmonic acid on potato node and protoplast culture. J. Plant Growth Regul., 11(1), 29–33. DOI: 10.1007/BF00193840

Ravnikar, M., Žel, J., Plaper, I., Špacapan, A. (1993). Jasmonic acid stimulates shoot and bulb formation of garlic in vitro. J. Plant Growth Reg., 12(2), 73–77. DOI: 10.1007/BF00193236

Sarkar, D., Pandey, S.K., Sharma, S. (2006). Cytokinins antagonize the jasmonates action on the regulation of potato (Solanum tuberosum) tuber formation in vitro. Plant Cell, Tissue Organ Cult., 87, 285–295. DOI: 10.1007/s11240-006-9166-3

Teixeira da Silva, J.A. (2012). Jasmonic Acid, but not Salicylic Acid, Improves PLB Formation of Hybrid Cymbidium. Plant Tissue Cult. Biotech., 22(2), 187–192. DOI: 10.3329/ptcb.v22i2.14209

Thomas, D. (2008). The role of activated charcoal in plant tissue culture. Biotechnol. Adv., 26, 618–631. DOI: 10.1016/j.biotechadv.2008.08.003

Tsai, S.J., Juengel, J.L., Wiltbank, M.C. (1997). Hormonal regulation of monocyte chemoattractant protein-1 messenger ribonucleic acid expression in corpora lutea. Endocrinology, 138, 4517–4520. DOI: 10.1210/endo.138.10.5577

Wang, G.L., Que, F., Xu, Z.S., Wang, F., Xiong, A.S. (2017). Exogenous gibberellin enhances secondary xylem development and lignification in carrot taproot. Protoplasma, 254, 839–848. DOI: 10.1007/s00709-016-0995-6

Yazawa, K., Takahata, K., Kamada, H. (2004). Isolation of the gene encoding carrot leafy cotyledon1 and expression analysis during somatic and zygotic embryogenesis. Plant Physiol. Biochem., 42(3), 215–223. DOI: 10.1016/j.plaphy.2003.12.003

Wilen, R.W., Mandel, R.M., Pharis, R.P., Holbrook, L.A., Moloney, M.M. (1990). Effects of abscisic acid and high osmoticum on storage protein gene expression in microspore embryos of Brassica napus. Plant Physiol., 94, 875–881. DOI: 10.1104/pp.94.3.875

Download

Published
2020-10-29



Tugce OZSAN 
Department of Horticulture, Faculty of Agriculture, Akdeniz University, Antalya, Turkey https://orcid.org/0000-0002-3265-6886
Elif Gulsun VURAL 
Department of Horticulture, Faculty of Agriculture, Akdeniz University, Antalya, Turkey
Ahmet Naci ONUS 
Department of Horticulture, Faculty of Agriculture, Akdeniz University, Antalya, Turkey https://orcid.org/0000-0001-8615-1480



License

 

Articles are made available under the conditions CC BY 4.0 (until 2020 under the conditions CC BY-NC-ND 4.0).
Submission of the paper implies that it has not been published previously, that it is not under consideration for publication elsewhere.

The author signs a statement of the originality of the work, the contribution of individuals, and source of funding.