EFFECT OF METHYL JASMONATE VAPORS ON LEVEL OF ANTHOCYANINS, BIOGENIC AMINES AND DECARBOXYLASES ACTIVITY IN SEEDLINGS OF CHOSEN VEGETABLE SPECIES
Marcin Horbowicz
Siedlce University of Natural Sciences and Humanities, SiedlceRyszard Kosson
Research Institute of Horticulture, SkierniewiceCezary Sempruch
Siedlce University of Natural Sciences and Humanities, SiedlceHenryk Dębski
Siedlce University of Natural Sciences and Humanities, SiedlceDanuta Koczkodaj
Siedlce University of Natural Sciences and Humanities, SiedlceAbstract
Seedlings of four vegetable species (maize, tomato, radish and onion) were treated for 7 days with methyl jasmonate (MeJA) vapors. MeJA accelerated senescence
process of plant tissues and accumulation of anthocyanins. The only exception were hypocotyls of radish, which was found to decrease of the anthocyanin content under the influence of MeJA. It has been shown that MeJA has a different impact on the content of free biogenic amines. In case of leaves and epicotyls of maize and tomato hypocotyls, MeJA had no effect on levels of putrescine (Put). The leaves of tomatoes have shown to increase the putrescine level as a result of the impact of MeJA vapors. However, in the tissues of radish and onion very large decline in putrescine and spermidine content under the influence of the phytohormone were observed. The presence of small amounts of spermine was found only in tissues of radish, and onion, which does not affect the use of MeJA. In tissues of maize the presence of a significant content of 2-phenylethylamine (PEA) were found. Use for 7 days of MeJA vapors resulted in 3-fold increase in the content of the PEA in maize leaves. Small levels of the amine has also been found in tomato hypocotyls, where use of MeJA caused reduction its content. Obtained results show a little
relationship between the activity of the studied enzymes (ornithine decarboxylase (ODC), lysine decarboxylase (LDC) and tyrosine decarboxylase (TYDC)) and the contents of the amines in seedlings of four vegetable species. No free cadaverine and tyramine were found, and therefore probably both polyamines might be present as conjugates. Putrescine also may be present in bound form. Moreover, since putrescine can be synthesized directly from ornithine or indirectly from arginine via agmatine, the activity of ODC alone did not give a full picture of the impact of MeJA on Put accumulation.
Keywords:
methyl jasmonate, seedlings, anthocyanins, amines, decarboxylasesReferences
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Biondi S., Scaramagli S., Capitani F., Altamura M.M., Torrigiani P., 2001. Methyl jasmonate upregulates biosynthetic gene expression, oxidation and conjugation of polyamines, and inhibits shoot formation in tobacco thin layers. J. Exp. Bot. 52, 231– 242.
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Chapple C.C.S., Walker M.A., Ellis B.E., 1986. Plant tyrosine decarboxylase can be strongly inhibited by L-α-aminooxy-β-phenylpropionate. Planta 167, 101–105.
Chen C.T., Chou C.M., Kao C.H., 1994. Methyl jasmonate induces the accumulation of putrescine but not proline in detached rice leaves. J. Plant Physiol. 143, 119–121.
Chen H., Jones A.D., Howe G.A., 2006. Constitutive activation of the jasmonate signaling pathway enhances the production of secondary metabolites in tomato. FEBS Lett. 580, 2540–2546.
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Facchini P.J., Huber-Allanach K.L., Tari L.W., 2000. Plant aromatic L-amino acid decarboxylases: evolution, biochemistry, regulation, and metabolic engineering applications. Phytochemistry 54, 121–138.
Farmer E.E., Almeras E., Krishnamurthy V., 2003. Jasmonates and related oxylipins in plant responses to pathogenesis and herbivory. Curr. Opin. Plant Biol. 6, 372–378.
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Groppa M.D., Benavides M.P., 2008. Polyamines and abiotic stress: recent advances. Amino Acids 34, 35–45.
Hahlbrock K., Grisebach H., 1979. Enzymatic controls in the biosynthesis of lignin and flavonoids. Ann. Rev. Plant Physiol. 30, 105–130.
Hahlbrock K., Scheel D., 1989. Physiology and molecular biology of phenylpropanoid metabolism. Annu. Rev. Plant Physiol. Plant Mol. Biol. 40, 347–369.
He Y., Fukushige H., Hildebrand D.F., Gan S., 2002. Evidence supporting a role of jasmonic acid in Arabidopsis leaf senescence. Plant Physiol. 128, 876–884.
Horbowicz M., Grzesiuk A., Dębski H., Koczkodaj D., Saniewski M., 2008. Methyl jasmonate inhibits anthocyanins synthesis in seedlings of common buckwheat (Fagopyrum esculentum Moench). Acta Biol. Crac. Series Bot. 50, 71–78.
Horbowicz M., Kosson R., Wiczkowski W., Koczkodaj D., Mitrus J., 2011a. The effect of methyl jasmonate on accumulation of 2-phenylethylamine and putrescine in seedlings of common buckwheat (Fagopyrum esculentum). Acta Physiol. Plant. 33, 897–903.
Horbowicz M., Wiczkowski W., Koczkodaj D., Saniewski M., 2011b. Effects of methyl jasmonate on accumulation of flavonoids in seedlings of common buckwheat (Fagopyrum esculentum Moench). Acta Biol. Hungar. 62, 265–278.
Horbowicz M., Kosson R., Saniewski M., Koczkodaj D., Mitrus J., 2013. Effects of simultaneously use of methyl jasmonate with other plant hormones on level of anthocyanins and biogenic amines in seedlings of common buckwheat (Fagopyrum esculentum Moench). Acta Agrobot. 66, 17–26.
Hung K.T., Kao C.H., 2004. Nitric oxide acts as an antioxidant and delays methyl jasmonate induced senescence of rice leaves. J. Plant Physiol. 161, 43–52.
Lee T-M., Lur H-S., Lin Y-H., Chu C., 1996. Physiological and biochemical changes related to methyl jasmonate-induced chilling tolerance of rice (Oryza sativa L.) seedlings. Plant, Cell Environ. 19, 65–74.
Lowry J.O.H., Rosebrough N.J., Farr A.L., Randal R.J., 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 256–277.
Mader J.C., 1999. Effects of jasmonic acid, silver nitrate and L-AOPP on the distribution of free and conjugated polyamines in roots and shoots of Solanum tuberosum in vitro. J. Plant Physiol. 154, 79–88.
Mancinelli A.L., 1984. Photoregulation of anthocyanin synthesis. VIII. Effects of light pretreatments. Plant Physiol. 75, 447–453.
Mancinelli A.L., 1990. Interaction between light quality and light quantity in the photoregulation of anthocyanin production. Plant Physiol. 92, 1191–1195.
Ngo T.T., Brillhart K.L., Davis R.H., Womg R.C., Bovaird J.H., Digangi J.J., Risov J.L., Marsh J.L., Phan A.P.H., Lenhoff H.M., 1987. Spectrophotometric assay for ornithine decarboxylase. Anal. Biochem. 160, 290–293.
Phan A.P.H., Ngo T.T., Lenhoff H.M., 1982. Spectrophotometric assay for lysine decarboxylase. Anal. Biochem. 120, 193–197.
Phan A.P.H., Ngo T.T., Lenhoff H.M., 1983. Tyrosine decarboxylase. Spectrophotometric assay and application determining pyridoxal-5’-phosphate. Appl. Biochem. Biotech. 8, 127–133.
Peremarti A., Bassie L., Yuan D., Palacho A., Christou P, Capell T., 2010. Transcriptional regulation of the rice arginine decarboxylase (Adc1) and S-adenosylmethionine decarboxylase (Samdc) genes by methyl jasmonate. Plant Physiol. Biochem. 48, 553–559.
Rabino I., Mancinelli A.L., 1986. Light, temperature and anthocyanins production. Plant Physiol. 81, 922–924.
Saniewski M., Horbowicz M., Puchalski J., 2006. Induction of anthocyanins accumulation by methyl jasmonate in shoots of Crassula multicava Lam. Acta Agrobot. 59, 43–50.
Saniewski M., Horbowicz M., Puchalski J., Ueda J., 2003. Methyl jasmonate stimulates the formation and the accumulation of anthocyanins in Kalanchoe blossfeldiana. Acta Physiol. Plant. 25, 143–149.
Saniewski M., Miszczak A., Kawa-Miszczak L., Wegrzynowicz-Lesiak E., Miyamoto K., Ueda J., 1998a. Effect of methyl jasmonate on anthocyanin accumulation, ethylene production, and CO2 evolution in cooled and uncooled tulip bulbs. J. Plant Growth Regul. 17, 33–37.
Saniewski M., Miyamoto K., Ueda J., 1998b. Methyl jasmonate induces gums and stimulates anthocyanin accumulation in peach shoot. J. Plant Growth Regul. 17, 121–124.
Shabana M., Gonaid M., Salama M.M., Abdel-Sattar E., 2006. Phenylalkylamine alkaloids from Stapelia hirsuta L. Nat. Prod. Res. 20, 710–714.
Shalaby A.R., 1996. Significance of biogenic amines to food safety and human health. Food Res. Inter. 29, 675–690.
Shan X., Zhang Y., Peng W., Xie D., 2009. Molecular mechanism for jasmonate-induction of anthocyanin accumulation in Arabidopsis. J. Exp. Bot. 60, 3849–3860.
Smith T.A., 1977. Phenethylamine and related compounds in plants. Phytochemistry 16, 9–18.
Steyn W.J., Wand S.J.E., Holcroft D.M., Jacobs G., 2002. Anthocyanins in vegetative tissues: proposed unified function in photoprotection. New Phytol. 155, 349–361.
Tamogami S., Rakwal R., Agrawal G.K., 2008. Interplant communication: airborne methyl jasmonate is essentially converted into JA and JA-Ile activating jasmonate signaling pathway and VOCs emission. Biochem. Biophys. Res. Commun. 376, 723–727.
Tieman D., Taylor M., Schauer N., Fernie A.R., Hanson A.D., Klee H.J., 2006. Tomato aromatic amino acid decarboxylases participate in synthesis of the flavor volatiles 2-phenylethanol and 2-phenylacetaldehyde. Proc. Natl. Acad. Sci. USA 103, 8287–8292.
Ueda J., Kato J., 1981. Promotive effect of methyl jasmonate on oat leaf senescence in the light. Z. Pflanzenphysiol., 103, 357–359.
Weidhase R.A.E., Kramell H.M., Lehmann J., Liebisch H.W., Lerbs W., Parthier B., 1987. Methyl jasmonate-induced changes in the polypeptide pattern of senescing barley leaf segments. Plant Sci. 51, 177–186.
Walters D.R., 2003. Polyamines and plant diseases. Phytochemistry 64, 97–107.
Wasternack C., 2007. Jasmonates: an update on biosynthesis, signal transduction and action in plant stress response, growth and development. Ann. Bot. 100, 681–697.
Xu B., Sheehan M.J., Timko M.P., 2004. Differential induction of ornithine decarboxylase (ODC) gene family members in transgenic tobacco (Nicotiana tabacum L. cv. Bright yellow 2) cell suspension by methyl-jasmonate treatment. Plant Growth Regul. 44, 101–116.
Bailey B.A., Strem M.D., Bae H., Antunez de Mayolo G., Guiltinan M.J., 2005. Gene expression in leaves of Theobroma cacao in response to mechanical ounding, ethylene, and/or methyl jasmonate. Plant Sci. 168, 1247–1258.
Biondi S., Scaramagli S., Capitani F., Altamura M.M., Torrigiani P., 2001. Methyl jasmonate upregulates biosynthetic gene expression, oxidation and conjugation of polyamines, and inhibits shoot formation in tobacco thin layers. J. Exp. Bot. 52, 231– 242.
Biondi S., Scoccianti V., Scaramagli S., Ziosi V., Torrigiani P., 2003. Auxin and cytokinin modify methyl jasmonate effects on polyamine metabolism and ethylene biosynthesis in tobacco leaf discs. Plant Sci. 165, 95–101.
Chalker-Scott L., 1999. Environmental significance of anthocyanins in plant stress responses. Photochem. Photobiol. 70, 1–9.
Chapple C.C.S., Walker M.A., Ellis B.E., 1986. Plant tyrosine decarboxylase can be strongly inhibited by L-α-aminooxy-β-phenylpropionate. Planta 167, 101–105.
Chen C.T., Chou C.M., Kao C.H., 1994. Methyl jasmonate induces the accumulation of putrescine but not proline in detached rice leaves. J. Plant Physiol. 143, 119–121.
Chen H., Jones A.D., Howe G.A., 2006. Constitutive activation of the jasmonate signaling pathway enhances the production of secondary metabolites in tomato. FEBS Lett. 580, 2540–2546.
Cheong J.J., Choi Y.D., 2003. Methyl jasmonate as a vital substance in plants. Trends Gen. 19, 409–413.
Close D.C., Beadle C.L., 2003. The ecophysiology of foliar anthocyanin. Bot. Rev. 69, 149–161.
Creelman R.A., Mullet J.E., 1995. Jasmonic acid distribution and action in plants: regulation during development and response to biotic and abiotic stress. Proc. Natl. Acad. Sci. USA 92, 4114–4119.
Facchini P.J., Huber-Allanach K.L., Tari L.W., 2000. Plant aromatic L-amino acid decarboxylases: evolution, biochemistry, regulation, and metabolic engineering applications. Phytochemistry 54, 121–138.
Farmer E.E., Almeras E., Krishnamurthy V., 2003. Jasmonates and related oxylipins in plant responses to pathogenesis and herbivory. Curr. Opin. Plant Biol. 6, 372–378.
Flores H.E., Galston A.W., 1982. Analysis of polyamines in higher plants by high performance liquid chromatography. Plant Physiol. 69, 701–706.
Gould K.S., 2004. Nature’s Swiss army knife: the diverse protective roles of anthocyanins in leaves. J. Biomed. Biotech. 2004, 314–320.
Groppa M.D., Benavides M.P., 2008. Polyamines and abiotic stress: recent advances. Amino Acids 34, 35–45.
Hahlbrock K., Grisebach H., 1979. Enzymatic controls in the biosynthesis of lignin and flavonoids. Ann. Rev. Plant Physiol. 30, 105–130.
Hahlbrock K., Scheel D., 1989. Physiology and molecular biology of phenylpropanoid metabolism. Annu. Rev. Plant Physiol. Plant Mol. Biol. 40, 347–369.
He Y., Fukushige H., Hildebrand D.F., Gan S., 2002. Evidence supporting a role of jasmonic acid in Arabidopsis leaf senescence. Plant Physiol. 128, 876–884.
Horbowicz M., Grzesiuk A., Dębski H., Koczkodaj D., Saniewski M., 2008. Methyl jasmonate inhibits anthocyanins synthesis in seedlings of common buckwheat (Fagopyrum esculentum Moench). Acta Biol. Crac. Series Bot. 50, 71–78.
Horbowicz M., Kosson R., Wiczkowski W., Koczkodaj D., Mitrus J., 2011a. The effect of methyl jasmonate on accumulation of 2-phenylethylamine and putrescine in seedlings of common buckwheat (Fagopyrum esculentum). Acta Physiol. Plant. 33, 897–903.
Horbowicz M., Wiczkowski W., Koczkodaj D., Saniewski M., 2011b. Effects of methyl jasmonate on accumulation of flavonoids in seedlings of common buckwheat (Fagopyrum esculentum Moench). Acta Biol. Hungar. 62, 265–278.
Horbowicz M., Kosson R., Saniewski M., Koczkodaj D., Mitrus J., 2013. Effects of simultaneously use of methyl jasmonate with other plant hormones on level of anthocyanins and biogenic amines in seedlings of common buckwheat (Fagopyrum esculentum Moench). Acta Agrobot. 66, 17–26.
Hung K.T., Kao C.H., 2004. Nitric oxide acts as an antioxidant and delays methyl jasmonate induced senescence of rice leaves. J. Plant Physiol. 161, 43–52.
Lee T-M., Lur H-S., Lin Y-H., Chu C., 1996. Physiological and biochemical changes related to methyl jasmonate-induced chilling tolerance of rice (Oryza sativa L.) seedlings. Plant, Cell Environ. 19, 65–74.
Lowry J.O.H., Rosebrough N.J., Farr A.L., Randal R.J., 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 256–277.
Mader J.C., 1999. Effects of jasmonic acid, silver nitrate and L-AOPP on the distribution of free and conjugated polyamines in roots and shoots of Solanum tuberosum in vitro. J. Plant Physiol. 154, 79–88.
Mancinelli A.L., 1984. Photoregulation of anthocyanin synthesis. VIII. Effects of light pretreatments. Plant Physiol. 75, 447–453.
Mancinelli A.L., 1990. Interaction between light quality and light quantity in the photoregulation of anthocyanin production. Plant Physiol. 92, 1191–1195.
Ngo T.T., Brillhart K.L., Davis R.H., Womg R.C., Bovaird J.H., Digangi J.J., Risov J.L., Marsh J.L., Phan A.P.H., Lenhoff H.M., 1987. Spectrophotometric assay for ornithine decarboxylase. Anal. Biochem. 160, 290–293.
Phan A.P.H., Ngo T.T., Lenhoff H.M., 1982. Spectrophotometric assay for lysine decarboxylase. Anal. Biochem. 120, 193–197.
Phan A.P.H., Ngo T.T., Lenhoff H.M., 1983. Tyrosine decarboxylase. Spectrophotometric assay and application determining pyridoxal-5’-phosphate. Appl. Biochem. Biotech. 8, 127–133.
Peremarti A., Bassie L., Yuan D., Palacho A., Christou P, Capell T., 2010. Transcriptional regulation of the rice arginine decarboxylase (Adc1) and S-adenosylmethionine decarboxylase (Samdc) genes by methyl jasmonate. Plant Physiol. Biochem. 48, 553–559.
Rabino I., Mancinelli A.L., 1986. Light, temperature and anthocyanins production. Plant Physiol. 81, 922–924.
Saniewski M., Horbowicz M., Puchalski J., 2006. Induction of anthocyanins accumulation by methyl jasmonate in shoots of Crassula multicava Lam. Acta Agrobot. 59, 43–50.
Saniewski M., Horbowicz M., Puchalski J., Ueda J., 2003. Methyl jasmonate stimulates the formation and the accumulation of anthocyanins in Kalanchoe blossfeldiana. Acta Physiol. Plant. 25, 143–149.
Saniewski M., Miszczak A., Kawa-Miszczak L., Wegrzynowicz-Lesiak E., Miyamoto K., Ueda J., 1998a. Effect of methyl jasmonate on anthocyanin accumulation, ethylene production, and CO2 evolution in cooled and uncooled tulip bulbs. J. Plant Growth Regul. 17, 33–37.
Saniewski M., Miyamoto K., Ueda J., 1998b. Methyl jasmonate induces gums and stimulates anthocyanin accumulation in peach shoot. J. Plant Growth Regul. 17, 121–124.
Shabana M., Gonaid M., Salama M.M., Abdel-Sattar E., 2006. Phenylalkylamine alkaloids from Stapelia hirsuta L. Nat. Prod. Res. 20, 710–714.
Shalaby A.R., 1996. Significance of biogenic amines to food safety and human health. Food Res. Inter. 29, 675–690.
Shan X., Zhang Y., Peng W., Xie D., 2009. Molecular mechanism for jasmonate-induction of anthocyanin accumulation in Arabidopsis. J. Exp. Bot. 60, 3849–3860.
Smith T.A., 1977. Phenethylamine and related compounds in plants. Phytochemistry 16, 9–18.
Steyn W.J., Wand S.J.E., Holcroft D.M., Jacobs G., 2002. Anthocyanins in vegetative tissues: proposed unified function in photoprotection. New Phytol. 155, 349–361.
Tamogami S., Rakwal R., Agrawal G.K., 2008. Interplant communication: airborne methyl jasmonate is essentially converted into JA and JA-Ile activating jasmonate signaling pathway and VOCs emission. Biochem. Biophys. Res. Commun. 376, 723–727.
Tieman D., Taylor M., Schauer N., Fernie A.R., Hanson A.D., Klee H.J., 2006. Tomato aromatic amino acid decarboxylases participate in synthesis of the flavor volatiles 2-phenylethanol and 2-phenylacetaldehyde. Proc. Natl. Acad. Sci. USA 103, 8287–8292.
Ueda J., Kato J., 1981. Promotive effect of methyl jasmonate on oat leaf senescence in the light. Z. Pflanzenphysiol., 103, 357–359.
Weidhase R.A.E., Kramell H.M., Lehmann J., Liebisch H.W., Lerbs W., Parthier B., 1987. Methyl jasmonate-induced changes in the polypeptide pattern of senescing barley leaf segments. Plant Sci. 51, 177–186.
Walters D.R., 2003. Polyamines and plant diseases. Phytochemistry 64, 97–107.
Wasternack C., 2007. Jasmonates: an update on biosynthesis, signal transduction and action in plant stress response, growth and development. Ann. Bot. 100, 681–697.
Xu B., Sheehan M.J., Timko M.P., 2004. Differential induction of ornithine decarboxylase (ODC) gene family members in transgenic tobacco (Nicotiana tabacum L. cv. Bright yellow 2) cell suspension by methyl-jasmonate treatment. Plant Growth Regul. 44, 101–116.
Marcin Horbowicz
Siedlce University of Natural Sciences and Humanities, Siedlce
Siedlce University of Natural Sciences and Humanities, Siedlce
Ryszard Kosson
Research Institute of Horticulture, Skierniewice
Research Institute of Horticulture, Skierniewice
Cezary Sempruch
Siedlce University of Natural Sciences and Humanities, Siedlce
Siedlce University of Natural Sciences and Humanities, Siedlce
Henryk Dębski
Siedlce University of Natural Sciences and Humanities, Siedlce
Siedlce University of Natural Sciences and Humanities, Siedlce
Danuta Koczkodaj
Siedlce University of Natural Sciences and Humanities, Siedlce
Siedlce University of Natural Sciences and Humanities, Siedlce
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