Abstrakt
Produkty genów małych białek szoku cieplnego (HSPB) wykazują aktywność chaperonową i neuroprotekcyjną, przyczyniając się do stabilizacji równowagi komórkowej i cytoszkieletu neuronów w warunkach stresu oksydacyjnego lub termicznego. Celem badań była chromosomowa lokalizacja pięciu genów małych białek szoku cieplnego (HspB1, HspB2, CRYAB – alternatywna nazwa HspB5, HspB6 i HspB8) u bydła i owiec, przy zastosowaniu techniki FISH z sondami uzyskanymi z klonów BAC (pochodzących z biblioteki genomowej CHORI-240 Bovine BAC Library) zawierających sekwencje tych genów. Przed hybrydyzacją in situ, przeprowadzoną na chromosomach metafazowych barwionych techniką prążków DAPI, potwierdzono obecność badanych genów w wyselekcjonowanych klonach metodą PCR z wykorzystaniem genowo specyficznych starterów. W wyniku przeprowadzonych eksperymentów uzyskano sygnały FISH w następujących regionach chromosomów bydła (BTA) i owiec (OAR): BTA25q22/OAR24q22 (HSPB1), BTA15q14-21/OAR15q14-21 (HSPB2 and CRYAB), BTA18q24/OAR14q24 (HSPB6), BTA17q24-25/OAR17q24-25 (HSPB8). Badania umożliwiły określenie fizycznej lokalizacji badanych genów na mapach genomowych tych gatunków i potwierdziły wysoki poziom konserwatyzmu autosomów u Bovidae. Uzyskane wyniki mogą dostarczyć przydanych informacji dotyczących genetycznego podłoża chorób neurodegeneracyjnych u zwierząt hodowlanych.
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