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ASPHC Baner

Vol. 20 No. 5 (2021)

Articles

Development of an efficient in vitro callus proliferation protocol for edible wild rhubarb (Rheum ribes L.)

DOI: https://doi.org/10.24326/asphc.2021.5.11
Submitted: 31 October 2020
Published: 29.10.2021

Abstract

Rheum ribes L. is a perennial wild species. Young shoots and flower bunches are freshly consumed, and root and rhizomes are generally used for medicinal purposes. The aim of the present study was to improve the callus proliferation protocol for R. ribes L. under in vitro conditions. For callus induction, hypocotyl explants taken from 14-day old plantlets germinated in Murashige and Skoog (MS) media were cultured in MS media with 9 plant growth regulator (PGR) combinations containing 6-benzylaminopurine (BAP) (2, 3, and 4 mg/L) + naphthylacetic acid (NAA) (0.1, 0.5, and 1 mg/L). Then, for callus proliferation, 4 PGR combinations containing NAA (0.2 mg/L) + thidiazuron (TDZ) (0.5, 1, 2, and 3 mg) were used in the first set of experiments, and 36 PGR combinations containing BAP (1, 2, 3, and 4 mg/L) + indole-3-butyric acid (IBA) (0.2, 0.5, and 1 mg/L), BAP (1, 2, 3, and 4 mg/L) + NAA (0.2, 0.5, and 1 mg/L), and TDZ (1, 2, 3, and 4 mg/L) + NAA (0.2, 0.5, and 1 mg/L) were used in the second set of experiments. At the end of the second set of experiments, the greatest callus regeneration ratios were obtained due to the combinations including BAP and IBA as well as the low-dose TDZ- (especially 1 mg/L) and NAA- (0.2, 0.5, 1 mg/L) combinations. Regarding callus fresh weights, TDZ + NAA combinations were found to be more successful. The greatest callus fresh weight (12.7 ±0.4 g) was obtained from MS medium supplemented with 2 mg/L TDZ and 0.2 mg/L NAA.

References

  1. Alaadin, A.M., Al-Khateeb, E.H., Jäger, A.K. (2007). Antibacterial activity of the Iraqi Rheum ribes. root. Pharm. Biol., 45(9), 688–690. https://doi.org/10.1080/13880200701575049
  2. Anjen, L., Bojian, B., Grabovskaya-Borodina, A.E., Hong, S.P., Mcneill, J., Mosyakin, S.L., Ohba, H., Park, C.W. (2003). Polygonaceae. In: Flora of China, vol 5, Wu, Z.Y., Raven, P.H., Hong, D.Y. (eds.). Science Press, Beijing, and Missouri Botanical Garden Press, St. Louis, 277–350.
  3. Chang, S.-J., Huang, S.-H., Lin, Y., Lin, Y.-J., Tsou, Y.-Y., Lin, C.-W. (2014). Antiviral activity of Rheum palmatum methanol extract and chrysophanol against Japanese encephalitis virus. Arch. Pharm. Res., 37, 1117–1123. https://doi.org/10.1007/s12272-013-0325-x
  4. Clapa, D., Borsai, O., Hartâ, M., Bonta, V., Szabo, K., Coman, V., Bobiş, O. (2020). Micropropagation, genetic fidelity and phenolic compound production of Rheum rhabarbarum L. Plants, 9(5), 656. https://doi.org/10.3390/plants9050656
  5. Cullen, J. (1967). Rheum L. In: Flora of Turkey and the East Aegean Islands, vol. 2. Davis, P.H. (ed.). Edinburgh University Press, 268–269.
  6. Çakılcıoğlu, U., Türkoğlu, I. (2009). Plants used for hemorrhoid treatment in Elazığ central district. Acta Hortic., 826, 89–96. https://doi.org/10.17660/ActaHortic.2009.826.11
  7. Farzami Sepehr, M., Ghorbanli, M. (2002). Effects of nutritional factors on the formation of anthraquinones in callus cultures of Rheum ribes. Plant Cell, Tissue Organ Cult., 68, 171–175. https://doi.org/10.1023/A:1013837232047
  8. Farzami Sepehr, M., Ghorbanli, M. (2005). Formation of catechin in callus cultures and micropropagation of Rheum ribes L. Pak. J. Biol. Sci., 8(10), 1346–1350. https://doi.org/10.3923/pjbs.2005.1346.1350
  9. Hu, B., Zhang, H., Meng, X., Wang, F., Wang, P. (2014). Aloe-emodin from rhubarb (Rheum rhabarbarum) inhibits lipopolysaccharide-induced inflammatory responses in RAW264.7 macrophages. J. Ethnopharmacol., 153(3), 846–853. https://doi.org/10.1016/j.jep.2014.03.059
  10. Kozak, D., Sałata, A. (2011). Effect of cytokinins on in vitro multiplication of rhubarb (Rheum rhaponticum L.) ‘Karpow Lipskiego’ shoots and ex vitro acclimatization and growth. Acta Sci. Pol. Hortorum Cultus, 10(4), 75–87.
  11. Malik, S., Sharma, N., Sharma, U.K., Singh, N.P., Bhushan, S., Sharma, M., Sinha, A.K., Ahuja, P.S. (2010). Qualitative and quantitative analysis of anthraquinone derivatives in rhizomes of tissue culture-raised Rheum emodi Wall. plants. J. Plant Physiol., 167(9), 749–756. https://doi.org/10.1016/j.jplph.2009.12.007
  12. Matsuda, H., Tewtrakul, S., Morikawa, T., Yoshikawa, M. (2004). Anti-allergic activity of stilbenes from Korean rhubarb (Rheum undulatum L.): structure requirements for inhibition of antigen-induced degranulation and their effects on the release of TNF-α and IL-4 in RBL-2H3 cells. Bioorg. Med. Chem., 12(18), 4871–4876. https://doi.org/10.1016/j.bmc.2004.07.007
  13. Mun, S.C., Mun, G.S. (2016). Development of an efficient callus proliferation system for Rheum coreanum Nakai, a rare medicinal plant growing in Democratic People’s Republic of Korea. Saudi J. Biol. Sci., 23(4), 488–494. https://doi.org/10.1016/j.sjbs.2015.05.017
  14. Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15, 473–497.
  15. Rajkumar, V., Guha, G., Kumar, R.A. (2011). Antioxidant and anti-cancer potentials of Rheum emodi rhizome extracts. Evid. Based Complement. Alternat. Med. https://doi.org/10.1093/ecam/neq048
  16. Rashid, S., Kaloo, Z.A., Singh, S., Bashir, I. (2014). Callus induction and shoot regeneration from rhizome explants of Rheum webbianum Royle – a threatened medicinal plant growing in Kashmir Himalaya. J. Sci. Innov. Res., 3(5), 515–518.
  17. Tabin, S., Kamili, A.N., Gupta, R.C. (2016). Novel study on in vitro culture of Rheum spiciforme Royle: an endangered medicinal plant of Gurez valley. Int. J. Curr. Res., 8(4), 28971–28979.
  18. Tabin, S., Kamili, A.N., Gupta, R.C., Parray, J.A., Bansal, A. (2018). In vitro culture of Rheum emodi Wall: an endangered medicinal plant of Northwestern Himalaya. Proc. Nation. Acad. Sci., India Sect. B Biol. Sci., 88(3), 995–1006. https://doi.org/10.1007/s40011-016-0835-7
  19. Tuncer, B., Günsan, B. (2017). Yabani ravent (Rheum ribes L.)’in doku kültürü ile çoğaltım olanakları üzerine araştırma. [Research on regeneration via tissue culture on wild rhubarb (Rheum ribes L.)]. Turk. J. Agric. Res., 4, 296–301 [in Turkish]. https://doi.org/10.19159/tutad.323431
  20. Wang, J., Lu, Y., Wang, Q, Liu, K., Song, Y., Bi, K. (2011). An efficient callus proliferation protocol and rhaponticin accumulation of Rheum franzenbachii Munt., a medicinal plant. J. Plant Biochem. Biotechnol., 20, 252–257. https://doi.org/10.1007/s13562-011-0055-4

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