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ASPHC Baner

Vol. 21 No. 4 (2022)

Articles

Localisation of bioactive compounds in the leaves of Hedera helix L. (Araliaceae) – a medical and cosmetic plant

DOI: https://doi.org/10.24326/asphc.2022.4.4
Submitted: September 23, 2021
Published: 2022-08-31

Abstract

Hedera helix (common ivy) is an attractive climbing and groundcover plant. Its leaves containing various bioactive compounds are used in medicine and cosmetology. The aim of this study was to present the functional leaf anatomy and histochemical analyses localising various secondary metabolites in ivy leaf blade
and petioles. Light and fluorescence microscopy and 8 histochemical tests and 3 fluorescence assays were
used. The leaf tissues contained phenolic compounds, phenolic acids, flavonoids, pectinous/mucilagous substances, acid lipids, essential oil, terpenoids, and steroid-containing terpenes. They are mainly responsible
for the biological and pharmacological activity of H. helix leaves. Most of these secondary metabolites were
present in the epidermis, mesophyll, phloem, and epithelial cells. In turn, they were not found in the xylem
and sclerenchyma tissues.

References

  1. Abdellatif, F., Boudjella, H., Zitouni, A., Hassani, A. (2014). Chemical composition and antimicrobial activity of the essential oil from leaves of Algerian Melissa officinalis L. EXCLI J., 13, 772–781.
  2. Ascensão, L., Mota, L., Castro, M. de M. (1999). Glandular trichomes on the leaves and flowers of Plectranthus ornatus: morphology, distribution and histochemistry. Ann. Bot., 84(4), 437–447. https://doi.org/10.1006/anbo.1999.0937 DOI: https://doi.org/10.1006/anbo.1999.0937
  3. Baker, J.R. (1966). Cytological Technique, 5th ed. Methven, London.
  4. Bezerra, L.D.A., Mangabeira, P.A.O., de Oliveira, R.A., Costa, L.C.D.B., Da Cunha, M. (2018). Leaf blade structure of Verbesina macrophylla (Cass.) F. S. Blake (Asteraceae): ontogeny, duct secretion mechanism and essential oil composition. Plant Biol., 20, 433–443. https://doi.org/10.1111/plb.12700 DOI: https://doi.org/10.1111/plb.12700
  5. Broda, B. (1971). Metody histochemii roślinnej. Państwowy Zakład Wydawnictw Lekarskich, Warszawa.
  6. Brundrett, M.C., Kendrick, B., Peterson, C.A. (1991). Efficient lipid staining in plant material with Sudan Red 7 B or Fluoral Yellow 088 in polyethylene glycol-glycerol. Biotech. Histochem., 66, 111–116. https://doi.org/10.3109/10520299109110562 DOI: https://doi.org/10.3109/10520299109110562
  7. Caissard, J-C., Joly C., Bergougnox V., Hugueney P., Mauriat M., Baudino S. (2004). Secretion mechanisms of volatile organic compounds in specialized cells of aromatic plants. Rec. Res. Develop. Cell Biol., 2, 1–15.
  8. Cerovic, Z.G., Ounis, A., Cartelat, A., Latouche, G., Goulas, Y., Meyer, S., Moya, I. (2002). The use of chlorophyll fluorescence excitation spectra for the non-destructive in situ assessment of UV-absorbing compounds in leaves. Plant Cell Environ., 25(12), 1663–1676. https://doi.org/10.1046/J.1365-3040.2002.00942.X DOI: https://doi.org/10.1046/j.1365-3040.2002.00942.x
  9. Charrière-Ladreix, Y. (1976). Répartition intracellulaire du secrétat flavonique de Populus nigra L. Planta 129, 167–174. DOI: https://doi.org/10.1007/BF00390024
  10. Chizzola, R. (2013). Regular monoterpenes and sesquiterpenes (essential oils). In: Natural products, Ramawat K.G., Mérillon, J.M. (eds). Springer, Berlin, 2973–3008. DOI: https://doi.org/10.1007/978-3-642-22144-6_130
  11. Chwil, M., Nurzyńska-Wierdak, R., Chwil, S., Matraszek, R., Neugebauerová, J. (2016). Histochemistry and micromorphological diversity of glandular trichomes in Melissa officinalis L. leaf epidermis. Acta Sci. Pol. Hortorum
  12. Cultus, 15(3), 153–172.
  13. Conn, H.J. (1977). Conn’s biological stains. A handbook on the nature and uses of the dyes employed in the biological laboratory. Waverly Press, Baltimore. David, R., Carde, J.P. (1964). Coloration différentielle des inclusions lipidique et terpéniques des pseudophylles du pin maritime au moyen du réactif Nadi. C. R. Acad. Sci. Paris, 258, 1338–1340.
  14. Dmitruk, M., Sulborska, A., Żuraw, B., Stawiarz, E., Weryszko‑Chmielewska, E. (2019). Sites of secretion of bioactive compounds in leaves of Dracocephalum moldavica L.: anatomical, histochemical, and essential oil study. Braz. J. Bot., 42, 701–715. https://doi.org/10.1007/s40415-019-00559-6 DOI: https://doi.org/10.1007/s40415-019-00559-6
  15. Evert, R.F. (2006). Esau’s plant anatomy: meristems, cells, and tissues of the plant body – their structure, function, and development, 3rd ed. John Wiley & Sons, Inc., Hoboken, New Jersey. http://doi.org/10.1002/0470047380 DOI: https://doi.org/10.1002/0470047380
  16. Fazio, S., Pouso, J., Dolinsky, D., Fernandez, A., Hernandez, M., Clavier, G., Hecker, M. (2009). Tolerance, safety and efficacy of Hedera helix extract in inflammatory bronchial diseases under clinical practice conditions: a prospective, open, multicentre postmarketing study in 9657 patients. Phytomedicine, 16(1), 17–24. https://doi.org/10.1016/j.phymed.2006.05.003 DOI: https://doi.org/10.1016/j.phymed.2006.05.003
  17. Ferreres, F., Figueiredo, R., Bettencourt, S., Carqueijeiro, I., Oliveira, J., Gil-Izquierdo, A., Pereira, D.M., Valentão, P., Andrade, P.B., Duarte, P., Barceló, A.R., Sottomayor, M. (2011). Identification of phenolic compounds in isolated vacuoles of the medicinal plant Catharanthus roseus and their interaction with vacuolar class III peroxidase: an H₂O₂ affair? J. Exp. Bot., 62(8), 2841–2854. https://doi.org/10.1093/jxb/erq458 DOI: https://doi.org/10.1093/jxb/erq458
  18. Gahan, P.B. (1984). Plant histochemistry and cytochemistry. Academic Press, London.
  19. Gabe, M. (1968). Techniques histologiques. Masson, Paris. Gaillard, Y., Blaise, P., Darré, A. Barbier, T., Pépin, G. (2003). An unusual case of death: suffocation caused by leaves of common ivy (Hedera helix). Detection of hederacosine C, α-hederin, and hederagenin by LC-EI/MS-MS. J. Anal. Toxicol., 27(4), 257–262. https://doi.org/10.1093/jat/27.4.257 DOI: https://doi.org/10.1093/jat/27.4.257
  20. Góra, J., Lis, A. (2005). Najcenniejsze olejki eteryczne. Wydawnictwo Uniwersytetu Mikołaja Kopernika, Toruń.
  21. Jabłońska-Trypuć, A., Czerpak, R. (2008). Surowce kosmetyczne i ich składniki. Część teoretyczna i ćwiczenia laboratoryjne.
  22. Med-Pharm, Wrocław.
  23. Jensen, W.A. (1962). Botanical histochemistry principles and practice, 1st ed. WH Freeman and Company, San Francisco.
  24. Johansen, D.A. (1940). Plant microtechnique. McGraw Hill, New York, 523.
  25. Kolalite, M.R., Oskolski, A.A., Richter, H.G., Schmitt, U. (2003). Bark anatomy and intercellular canals in the stem of elarbrea paradoxa (Araliaceae). IAWA J., 24(2): 139–154. DOI: https://doi.org/10.1163/22941932-90000327
  26. Karabourniotis, G., Liakopoulos, G. (2005). Phenolic compounds in plant cuticles: physiological and ecophysiological aspects. In: Advances in plant physiology, vol. 8, Hemantaranjan, A. (ed.). Scientific Publishers, Jodhpur, India, 33–47.
  27. Karabourniotis, G., Liakopoulos, G., Nikolopoulos, D., Bresta, P., Stavroulaki, V., Sumbele, S. (2014). “Carbon gain vs. water saving, growth vs. defence”: two dilemmas with soluble phenolics as a joker. Plant Sci. 227, 21–27. https://doi.org/10.1016/j.plantsci.2014.06.014 DOI: https://doi.org/10.1016/j.plantsci.2014.06.014
  28. Kelayeh, T.P.S., Abedinzade, M., Ghorbani, A. (2019). A review on biological effects of Lamium album (white dead nettle) and its components. J. Herbmed Pharmacol., 8(3), 185–193. https://doi.org/10.15171/jhp.2019.28 DOI: https://doi.org/10.15171/jhp.2019.28
  29. Kohlmünzer, S. (2016). Farmakognozja. Podręcznik dla studentów farmacji. Wydawnictwo Lekarskie PZWL, Warszawa.
  30. Konyar, S.T., Öztürk, N., Dane, F. (2014). Occurence, types and distribution of calcium oxalate crystals in leaves and stems od some species of poisonous plants. Bot. Stud., 55(32). https://doi.org/10.1186/1999-3110-55-32 DOI: https://doi.org/10.1186/1999-3110-55-32
  31. Korszun, S., Bykowska, J. (2011). Flowering plants of Hedera helix L. in the Grunwald district of the city of Poznań. Acta Agrobot., 64 (1): 45–56. DOI: https://doi.org/10.5586/aa.2011.006
  32. Kromer, K., Kreitschitz, A., Kleinteich, T., Gorb, S.N., Szumny, A. (2016). Oil secretory system in vegetative organs of three Arnica taxa: essential oil synthesis, distribution and accumulation. Plant Cell Physiol., 57(5), 1020–1037. https://doi.org/10.1093/pcp/pcw040 DOI: https://doi.org/10.1093/pcp/pcw040
  33. Kruttschnitt, E., Wegener, T., Zahner, C., Henzen-Bücking, S. (2020). Assessment of the efficacy and safety of ivy leaf (Hedera helix) cough syrup compared with acetylcysteine in adults and children with acute bronchitis. Evid. Based Complement. Alternat. Med., 1910656. https://doi.org/10.1155/2020/1910656 DOI: https://doi.org/10.1155/2020/1910656
  34. Lamer-Zarawska, E., Kowal-Gierczak, B., Niedworok, J. (ed). (2012). Fitoterapia i leki roślinne. Wydawnictwo Lekarskie PZWL, Warszawa.
  35. Lipiński, M. (2010). Pożytki pszczele, zapylanie i miododajność roślin. Państwowe Wydawnictwo Rolnicze i Leśne, Warszawa.
  36. Lulai, E.C., Morgan, W.C. (1992). Histochemical probing of potato periderm with neutral red: a sensitive cytofluorochrome for the hydrophobic domain of suberin. Biotech. Histochem. 67, 185–195. https://doi.org/10.3109/10520299209110065 DOI: https://doi.org/10.3109/10520299209110065
  37. Lutsenko, Y., Bylka, W., Matławska, I., Darmohray, R. (2010). Hedera helix as a medicinal plant. Herba Pol., 56(1), 83–96.
  38. Mace, M.E., Bell, A.A., Stipanovic, R.D. (1974). Histochemistry and isolation of gossypol and related terpenoids in roots of cotton seedlings. Phytopathology, 64, 1297–1302. DOI: https://doi.org/10.1094/Phyto-64-1297
  39. Maddox, V., Westbrooks, R., Byrd, J.D. (2018). English Ivy (Hedera helix L.). Tech. Rep. https://doi.org/10.13140/RG.2.2.20982.24649
  40. Metcalfe, C.R., Chalk, L. (1972). Anatomy of the dicotyledons. Vol. 2. Oxford Press, London, UK.
  41. Nakata, P.A., Kostman, T.A. and Franceschi, V.R. (2003). Calreticulin is enriched in the crystal idioblasts of Pistia stratiotes. Plant Physiol. Biochem., 41(5), 425–430. https://doi.org/10.1016/S0981-9428(03)00049-4 DOI: https://doi.org/10.1016/S0981-9428(03)00049-4
  42. Nowiński, M. (1977). Dzieje roślin i upraw ogrodniczych. Państwowe Wydawnictwo Rolnicze i Leśne, Warszawa.
  43. O’Brien, T.P., McCully, M.E. (1981). The study of plant structure: principles and selected methods. Termarcarphi Pty Ltd., Melbourne.
  44. Ostroumova, T.A., Oskolski, A.A. (2010). Survey of the leaf anatomy of Araliaceae and some related taxa. Plant Div. Evol., 128(3–4), 423–441. https://doi.org/10.1127/1869-6155/2010/0128-0020 DOI: https://doi.org/10.1127/1869-6155/2010/0128-0020
  45. Parimal, K., Khale, A., Pramod, K. (2011). Resin from herbal origin and a focus on their appliactions. Int. J. Pharm. Sci. Res., 2(5), 1077–1085.
  46. Petra, S.A., Georgescu, M.I., Manescu, C.R., Toma, F., Badea, M.L., Dobrescu, E., Popa, V.I. (2020). Leaves anatomical and physiological adaptations of Vinca major ‘Variegata’ and Hedera helix L. to specific roof garden conditions. Not. Bot. Horti Agrobot. Cluj Napoca, 47(4), 318–328. https://doi.org/10.15835/nbha48111784 DOI: https://doi.org/10.15835/nbha48111784
  47. Rioux, D., Nicole, M., Simard, M., Ouellette, G.B. (1998). Immunocytochemical evidence that secretion of pectin occurs during gel (gum) and tylosis formation in trees. Phytopathology, 88, 494–505. https://doi.org/10.1094/PHYTO.1998.88.6.494 DOI: https://doi.org/10.1094/PHYTO.1998.88.6.494
  48. Rostafiński, J., Siedl, O. (1962). Przewodnik do oznaczania roślin. PWRiL, Warszawa.
  49. Salinas, M.L., Ogura, T., Soffchi, L. (2001). Irritant contact dermatitis caused by needle-like calcium oxalate crystals, raphides, in Agave tequilana among workers in tequila distilleries and agave plantations. Contact Derm., 44, 94–96. https://doi.org/10.1034/j.1600-0536.2001.440208.x DOI: https://doi.org/10.1034/j.1600-0536.2001.440208.x
  50. Săvulescu, E., Luchian, V. (2009). Comparative anatomy of the vegetative organs of the Hedera helix L. (Araliaceae). Scientific Papers, series A, vol. LII. USAMV Bucharest, 487–490.
  51. Seneta, W., Dolatowski, J. (2007). Dendrologia. WN PWN, Warszawa.
  52. Solovchenko, A., Merzlyak, M. (2003). Optical properties and contribution of cuticle to UV protection in plants: experiments with apple fruit. Photochem. Photobiol. Sci., 2(8), 861–866. https://doi.org/10.1039/b302478d DOI: https://doi.org/10.1039/b302478d
  53. Strzelecka, H., Kowalski, J. (2000). Encyklopedia zielarstwa i ziołolecznictwa. Państwowe Wydawnictwo Naukowe, Warszawa.
  54. Sulborska, A., Konarska, A., Matysik-Woźniak, A., Dmitruk, M., Weryszko-Chmielewska, E., Skalska-Kamińska, A., Rejdak, R. (2020). Phenolic constituents of Lamium album L. subsp. album flowers: anatomical, histochemical, and phytochemical study. Molecules 25, 6025. https://doi.org/10.3390/molecules25246025 DOI: https://doi.org/10.3390/molecules25246025
  55. Szweykowska, A., Szweykowski, J. (ed). (2003). Słownik botaniczny. Wiedza Powszechna, Warszawa.
  56. Sǎvulescu, E., Luchian, V. (2009). Comparative anatomy of the vegetative organs of the Hedera helix L., (Araliaceae). Scientific Papers, Series A, USAMV Bucharest, 52, 487–490.
  57. Ščepánková, I., Hudák, J. (2004). Leaf and tepal anatomy, plastid ultrastructure and chlorophyll content in Galanthus nivalis L. and Leucojum aestivum L. Plant Syst. Evol., 243(3–4), 211–219. https://doi.org/10.1007/s00606-003-0086-y DOI: https://doi.org/10.1007/s00606-003-0086-y
  58. Tantry, M.A., Bhat, G.A., Idris, A., Dar, J.A., Al Omar, S.Y., Masoodi, K.Z., Bashir, A., Ganai, B.A., Kamili, A.N., Shawl, A.S. (2014). Sulfated triterpenes from Lemon balm. Helv. Chim. Acta, 97, 1497–1506. https://doi.org/10.1002/hlca.20140 0001 DOI: https://doi.org/10.1002/hlca.201400001
  59. Van Wyk, B-E., Wink, M. (2004). Medicinal plants of the world. An illustrated scientific guide to important medicinal plants and their uses. Timber Press, Portland, Oregon.
  60. Volk, G.M., Lynch‐Holm, V., Kostman, T.A., Franceschi, V.R. (2002). The role of druse and raphide calcium oxalate crystals in tissue calcium regulation in Pistia stratiotes leaves. Plant Biol., 4(1), 34–45. https://doi.org/10.1055/s-2002-20434 DOI: https://doi.org/10.1055/s-2002-20434
  61. Zheng, P., Yagami, S., Aoki, D., Yoshida, M., Sano, Y., Matsushita, Y., Fukushima, K. (2017). The composition and chemical alteration of gums in the vessels of Phellodendron amurense. Holzforschung, 71(12), 969–976. https://doi.org/10.1515/hf-2017-0057 DOI: https://doi.org/10.1515/hf-2017-0057
  62. Zeng, J., Huang, T., Xue, M., Chen, J., Feng, L., Du, R., Feng, Y. (2018). Current knowledge and development of hederagenin as a promising medicinal agent: a comprehensive review. RSC Adv., 8, 24188. https://doi.org/10.1039/c8ra03666g DOI: https://doi.org/10.1039/C8RA03666G
  63. Zhai, Ch., Wang, M., Raman, V., Rehman, J.U., Meng, Y., Zhao, J., Avula, B., Wang, Y-H., Tian, Z., Khan, I.A. (2017). Eleutherococcus senticosus (Araliaceae) leaf morpho-anatomy, essential oil composition, and its biological activity against Aedes aegypti (diptera: Culicidae). J. Med. Entomol., 54(3), 658–669. https://doi.org/10.1093/jme/tjw221 DOI: https://doi.org/10.1093/jme/tjw221

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