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Vol. 13 No. 4 (2014)

Articles

In vitro FLOWERING AND MICROPROPAGATION OF LISIANTHUS (Eustoma grandiflorum) IN RESPONSE TO PLANT GROWTH REGULATORS (NAA AND BA)

Submitted: November 27, 2020
Published: 2014-08-31

Abstract

In vitro flowering and micropropagation are useful for plant breeding programs and commercial production of important ornamental plants. In vitro conditions including media components, kind, concentration and ratio of plant growth regulators and culture conditions significantly affect in vitro flowering and micropropagation. There is no any report dealing with the in vitro flowering of Lisianthus (Eustoma grandiflorum). Here, a protocol was developed for flowering and high frequency in vitro micropropagation of E. grandiflorum, an ornamental plant. Micropropagation is an effective tools for propagation of ornamental plants in large scale. The aim of the present study was to evaluate the effect of different concentrations of NAA and BA on micropropagation and flowering of Lisianthus, in vitro. Used culture medium was MS enriched with 0, 0.1, 0.2 and 2 mg L-1 of NAA and BA. In establishment process of explants, the most shoot length (2.07 cm per
plant) was obtained on medium supplemented with 0.1 mg L-1 BA (without NAA). Maximum shoot number (5.80 per plant) was produced in medium containing 0.1 mg L-1 BA along with 0.2 mg L-1 NAA. Bud explants in culture media containing 0.2 mg L-1 NAA (without BA) and 0.1 mg L-1 NAA along with 2 mg L-1 BA produced maximum node number (3.20 per plant). The largest number of root (14.53 per plant) and root length (3.87 cm per plant) were produced on 0.2 mg L-1 NAA without BA, also 0.2 mg L-1 BA plus 0.2 mg L-1 NAA and 0.2 mg L-1 BA without NAA. Explants produced flower on medium containing 0.1 mg L-1 BA along with 0.1 mg L-1 NAA without transition of callus formation. Flower was produced from callus in medium containing 0.1 mg L-1 BA along with 2 mg L-1 NAA. Regenerated plants showed 98% survival in greenhouse during acclimatization. Acclimatized plants were morphologically similar to the mother plants.

References

Ahmadi Hesar A., Kaviani B., Tarang A.R., Bohlooli S., 2011. Effect of different concentrations of kinetin on regeneration of ten weeks (Matthiola incana). Plant Omics J., 4(5), 236–238.
Chaari-Rkhis A., Maalej M., Ouled Messaoud S., Drira N., 2006. In vitro vegetative growth and flowering of olive tree in response to GA3 treatment. Afr. J. Biotech., 5(22), 2097–2302.
Chawla H.S., 2009. Introduction to plant biotechnology. Springer.
Dewir Y., Chakrabart D., Ali M., Singh N., Hahn E., Paek K., 2007. Influence of GA3, sucrose and solid medium/bioreactor culture on in vitro flowering of Spathiphyllum and association of glutathione metabolism. Plant Cell, Tiss. Organ Cult., 90, 225–235.
Dielen V., Lecouvet V., Dupont S., Kinet J., 2001. In vitro control of floral transition in tomato (Lycopersicon esculentum Mill.), the model for autonomously flowering plants, using the late flowering uniflora mutant. J. Exp. Bot., 52(357), 715–723.
Fukai S., Miyata H., Goi M., 1996. Factors affecting adventitious shoot regeneration from leaf explants of prairie gentian (Eustoma grandiflorum (Raf.) Shinners). Technic. Bull. Fac. Agric-Kagawa Univ., 48(2), 103–109.
Fuller M.P., Fuller F.M., 1995. Plant tissue culture using Brassica seedlings. J. Biol. Edu., 20(1), 53–59.
Gautam V.K., Mittal A., Nanda K., Gupta S.C., 1983. In vitro regeneration of plantlets from somatic explants of Matthiola incana. Plant Sci. Lett., 29, 25–32.
Ghaffari Esizad S., Kaviani B., Tarang A.R., Bohlooli Zanjani S., 2012. Micropropagation of lisianthus, an ornamental plant. Plant Omics J., 5, 314–319.
Gomes F., Simões M., Lopes M.L., Canhoto M., 2010. Effect of plant growth regulators and genotype on the micropropagation of adult trees of Arbutus unedo L. (strawberry tree). New Biotech., 27, 882–892.
Hammaudeh H.Y., Suwwan M.A., Abu Quoud H.A., Shibli R.A., 1998. Micropropagation and regeneration of Honeoye strawberry. Dirasat Agric. Sci., 25, 170–178.
Han B.H., Yu H.J., Yae B.W., Peak K.Y., 2004. In vitro micropropagation of Lilium ‘Georgia’ by shoot formation as influenced by addition of liquid medium. Sci. Hortic., 103, 39–49.
Harbaugh B.K., 2006. Lisianthus, Eustoma grandiflorum. In: flower breeding and genetics, Anderson N.O. (ed.). Springer, Netherlands, 645–663.
Hartmann H.J., Kester D.E., Davies F.T., Geneve R.T., 1997. Plant propagation: principle and practices, 6th ed. Prentica-Hill, Englewood Cliffs, NJ.
Hashemabadi D., Kaviani B., 2010. In vitro proliferation of an important medicinal plant Aloe-A method for rapid production. Aus. J. Crop Sci., 4(4), 216–222.
Huang X., Yang B., Hu C., Yao J., 2009. In vitro induction of inflorescence in Dioscorea zingiberensis. Plant Cell. Tiss. Org. Cult., 99, 209–215.
Jain S.M., Ochatt S.J., 2010. Protocols for in vitro propagation of ornamental plants. Springer Protocols, Humana Press.
Kalimuthu K., Senthilkumar R., Vijayakumar S., 2007. In vitro micropropagation of orchid, Oncidium sp. (Dancing Dolls). Afr. J. Biotech., 6(10), 1171–1174.
Kaviani B., Ahmadi Hesar A., Kharabian Masouleh A., 2011. In vitro propagation of Matthiola incana (Brassicaceae)-an ornamental plant. Plant Omics J., 4(7), 435–440.
Kulpa D., Nowak N., 2011. In vitro flowering of Petunia × atkinsiana D. Don. Folia Hortic. 23/2, 125–129.
Kunitake H., Nakashima T., Mori K., Tanaka M., Mii M., 1995. Plant regeneration from mesophyll protoplasts of lisianthus (Eustoma grandiflorum) by adding activated charcoal into protoplast culture medium. Plant Cell. Tiss. Org. Cult., 43, 59–65.
Lee-Epinosa H.E., Murguia-Gonzalez J., Garcia-Rosas B., Cordova-Contreras A.L., Laguna C., 2008. In vitro clonal propagation of vanilla (Vanilla planifolia Andrews). HortSci. 43, 454–58.
Lin Ch.S., Liang C.J., Hsaio H.W., Lin M.J., Chang W.C., 2007. In vitro flowering of green and albino Dendrocalamus latiflorus. New Forests., 34(2), 177–186.
Masmoudi-Alloche F., Meziou B., Kriaâ W., Gargouri-Bouzid R., Drira N., 2010. In vitro flowering induction in date palm (Phoenix dactylifera L.). J. Plant Growth Reg., 29(1), 35–43.
Ming-xia G., et al., 2008. Study on rapid propagation technology of Eustoma grandiflorum in vitro. J. Anhui Agric. Sci., Abstract, 9.
Mousavi E.S., Behbahani M., Hadavi E., Miri S.M., Karimi N., 2012. Plant regeneration in Eustoma grandiflorum axillaries buds (Gentinaceae). Trakia J. Sci., 10(2), 75–78.
Murashige T., Skoog F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant, 15, 473–497.
Nhut D.T., Hai N.T., Phan M.X., 2010. A highly efficient protocol for micropropagation of Begonia tuberous. In: Protocols for in vitro propagation of ornamental plants, Jain S.M., Ochatt S.J. (eds). Springer Protocols, Humana Press, 15–20.
Ordogh M.‚ Jambor-Benczur E., Tilly-Mandy A., 2006. Micropropagation of ‘Echo’ cultivars of Eustoma grandiflorum (ISHS). Acta Horticult.: V International Symposium on In Vitro Culture and Horticultural Breeding, 725, 457–460.
Paek K.Y., Hahn E.J., 2000. Cytokinins, auxins and activated charcoal affect organogenesis and anatomical characteristics of shoot-tip cultures of lisianthus (Eustoma grandiflorum (Raf.) Shinn). In Vitro Cell Dev. Biol-Plant, 36, 128–132.
Pati P.K., Rath S.P., Sharma M., Sood A., Ahuja P., 2005. In vitro propagation of rose – a review. Biotech. Adv., 24, 94–114.
Semeniuk P., Griesbach R.J., 1987. In vitro propagation of prairie gentian. Plant Cell Tiss. Org. Cult., 8, 249–253.
Taylor N.J., Van Staden J., 2006. Towards an understanding of in vitro flowering. In: Floriculture, ornamental and plant biotechnology: advances and topical issues, Da Silva J.A.T. (ed.). Global Sci. Book, London, 2, 1–22.
Tee C.S., Maziah M., Tan S.C., 2008. Induction of in vitro flowering in the orchid Dendrobium Sonia 17. Biol. Plant., 52(4), 723–726.
Van Staden J., Zazimalova E., George E.F., 2008. Chapter 6: Plant growth regulators, II: Introduction; cytokinins, their analogues and inhibitors. In: Plant propagation by tissue culture, 3rd edition. Vol. 1., George E.F., Hall M.A., De Klerk G.J. (eds). Springer, Dordrecht, The Netherlands, 205–226.
Wang G.Y., Yuan M.F., Hong Y., 2002. In vitro flower induction in roses. In Vitro Cell. Dev. Biol. Plant. 38, 513–518.
Xilin H., 1992. Effect of different cultivars and hormonal conditions on strawberry anther culture in vitro. J. Nanjing Agric. Univ., 15, 21–28.
Xue-hua J., Wei Y., You-lin L., Xiang-ying K., Xiu-chun P., 2009. Aseptic seeding and establishment of plantlet regeneration system in Eustoma grandiflorum. J. Anhui Agric. Sci. Abstract, 5.
Zhang Z., Leung D., 2002. Factors influencing the growth of micropropagated shoots and in vitro flowering of gentian. Plant Growth Reg., 36, 245–250.

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