Abstract
The effect of auxins: IAA, IBA and NAA in concentrations of 1.0, 2.5 and 5.0 mg·dm-3 on rooting of Hebe buchananii and Hebe canterburiensis ‘Prostrata’ in vitro
was examined. Shoots used in the experiment were excised from aseptically grown shoots on 1/2 MS media. Auxins used in the experiment were showed to have a positive effect on rooting of shoots of Hebe buchananii and Hebe canterburiensis ‘Prostrata’ in vitro. The biggest number of rooted microshoots and the longest roots of Hebe buchananii formed in presence of IBA. On the media supplemented with IAA in concentrations of 2.5 and 5.0 mg·dm-3, IBA in concentration of 5 mg·dm-3 and NAA in concentration of 1 mg·dm-3 100% of rooted shoots of Hebe canterburiensis ‘Prostrata’ were obtained. The biggest number of roots occurred on media with 2.5 and 5.0 mg IAA·dm-3. NAA added to the media caused callusing of the shoots base.
References
Armstrong T. T. J., De Lange P. J., 2005. Conservation genetics of Hebe speciosa (Plantaginaceae) an endangered New Zealand shrub. Bot. J. Linnean Soc. 149, 229–239.
Cacho M., Moran M., Herrera M. T., Fernandez-Tarrago J., 1991. Morphogenesis in leaf, hypocotyl and root explants of Digitalis thapsi L. cultured in vitro. Biom. Life Sci. 25, 2, 117–123.
Cervelli C., 2001. New species for growing outside in containers. Informatore Agrario 57, 33, 65–70.
Dbski M., 2002. Badania nad ukorzenianiem mikrosadzonek wybranych gatunków roślin ozdobnych. Rozpr. Nauk. AR w Lublinie, z. 262.
De Paiva Neto V. B., Da Mota T. R., Otoni W. C., 2003. Direct organogenesis from hypocotylderived explants of annatto (Bixa orellana). Plant Cell, Tissue and Organ Cult. 75, 159–167.
Gopi C., Nataraja Sekhar Y., Ponmurugan P., 2006. In vitro multiplication of Ocimum gratissimum L. through direct regeneration. Afr. J. Biotechnol. 5 (9), 723–726.
Jankiewicz L. S., red., 1997. Regulatory wzrostu i rozwoju roślin. Właściwości i działanie. Wyd. Nauk. PWN Warszawa, 17–40.
Kristensen L. N., 1989. Hebe cultivars as potential new pot plants. Acta Hort. 252, 235–238.
Marosz A., 2006. Hebe. Szkółkarstwo 1(65), 5–9.
Murashige T., Skoog F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15, 473–497.
Świstowska A., Kozak D., 2004. Wpływ auksyn na ukorzenianie mikrosadzonek i adaptację roślin Columnea hirta Klotzsch et Hanst. Cz. I. W kulturze in vitro. Acta Sci. Pol., Hortorum Cultus 3(2), 229–238.
Świstowska A., Kozak D., 2005. Wpływ auksyn na ukorzenianie mikrosadzonek i adaptację roślin Nematanthus × hybridus 'Tropicana'. Zesz. Probl. Post. Nauk Rol. 504, 307–312.
Tiwari V., Singh B. D., Tiwari K. N., 1998. Shoot regeneration and somatic embryogenesis from different explants of Brahmi [Bacopa monniera (L.) Wettst.]. Plant Cell Rep. 17, 538–543.
Wawrosch C., Zeitlhofer P., Grauwald B., Kopp B., 2003. Effects of rooting chemicals on the establishment of micropropagated Picrorhiza kurroa plantlents in the greenhouse. Acta Hort. 616, 271–274.
Downloads
Download data is not yet available.