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ASPHC Baner

Vol. 23 No. 6 (2024)

Articles

First report of Robustodorus subtenuis on garlic plants in Poland

DOI: https://doi.org/10.24326/asphc.2024.5435
Submitted: September 14, 2024
Published: 2024-12-20

Abstract

During the observation of a garlic plantation, a sample consisting of plants with symptoms of growth inhibition and the soil surrounding the roots was collected. Phytophagous nematodes were isolated from the soil sample as well as the garlic plant shoots, with an average density of 75 individuals per 100 mL. Microscope slides were prepared from adult individuals and subjected to morphological and morphometric analysis, as well as DNA examination. It was demonstrated that the examined nematodes belong to the species Robustodorus subtenuis. Both the female and male nematodes differed from individuals previously found in the cultivation of Narcissus and Gladiolus. On average, R. subtenuis females have shorter bodies and longer tails, with the averages calculated for the nematode population from the soil being slightly lower than the average values for nematodes from the garlic shoots. Additionally, the females found in the soil had a shorter distance from the lip region to the vulva and a shorter post-vulval sac, the length of which constituted 32 to 45% of the distance from the vulva to the anus. On average, the male bodies were shorter and had longer tails. They were also wider at the level of the anus. The average body length of males isolated from the soil, like that of the females, was lower than the average for individuals isolated from the garlic shoots. Variations in the influence of the host plant on the morphometry of the parasitic nematodes were observed. This is the first report of R. subtenuis colonizing garlic plants in Poland.

References

  1. Allen, M.W. (1952). Taxonomic status of the bud and leaf nematodes related to Aphelenchoides fragariae (Ritzema Bos, 1891). Proc. Helminthol. Soc. Wash., 19, 108–120.
  2. Andrássy, I. (2007). Free-living nematodes of Hungary (Nematoda errantia). Vol. II. Pedozoologica Hungarica No. 4.
  3. Csuzdi, C., Mahunka, S. (eds.). Budapest, Hungary, Hungarian Natural History Museum and Systematic Zoolo-gy Research Group of the Hungarian Academy of Sciences.
  4. Baranovskaya, I.A. (1981). [Plant and soil nematodes (Aphelenchoiodidae and Seinuridae)]. Nauka, Moskwa, 234 pp.
  5. Baranowski, T. (1976). Badania nad szkodliwą fauną złocieni w okolicach Poznania. Rocz. Nauk Rol., ser. E, 6(1), 19–23.
  6. Bélair, G., Forge, T., Mimee, B., Tenuta, M., Yu, Q. (2018). Current state of plant parasitic nematodes in Canada. In: Plant parasitic nematodes in sustainable agriculture of North America, Sustainability in plant and crop pro-tection, Subbotin, S.A. Chitambar J.J. (eds.). Springer, Cham, 1–30. https://doi.org/10.1007/978-3-319-99585-4_1
  7. Bird, G.W., Abawi, G.S., La Mondia, J.A. (2018). Plant parasitic nematodes of New York, New Jersey and Pennsyl-vania. In: Plant parasitic nematodes in sustainable agriculture of North America, sustainability in plant and crop protection, Subbotin, S.A. Chitambar, J.J. (eds.). Springer, Cham, 27–55. https://doi.org/10.1007/978-3-319-99588-5_2
  8. Burke, D.J., Hoke, A.J., Koch, J. (2020). The emergence of beech leaf disease in Ohio: probing the plant microbiome in search of the cause. For. Pathol., 50, e12579. https://doi.org/10.1111/efp.12579
  9. CABI. 2024. https://www.cabidigitallibrary.org
  10. Carta, L.K., Handoo, Z.A., Li, S., Kantor, M., Bauchan, G., McCann, D., Gabriel, C.K., Yu, Q., Reed, S., Koch, J., Martin, D., Burke, D.J. (2020). Beech leaf disease symptoms caused by newly recognized nematode subspecies Litylenchus crenatae mccannii (Anguinata) described from Fagus grandifolia in North America. For. Pathol., 50, e12580. https://doi.org/10.1111/efp.12580
  11. Chałańska, A., Bogumił, B., Machnicka, K., Dzięgielewska, M. (2014). First record of chrysanthemum foliar nema-tode Aphelenchoides ritzemabosi (Schwarz 1911) Steiner & Buhrer 1932 (Nematoda: Aphelechoididae) in leaf buds of black currant (Ribes nigrum L.) in Poland. Prog. Plant Prot., 54(4), 403–406. http://dx.doi.org/10.14199/ppp-2014-068
  12. Chałańska, A., Łabanowski, G., Maciorowski, R. (2013). Control efficacy of selected natural products against chry-santhemum foliar nematode – Aphelenchoides ritzemabosi (Schwartz, 1911) Steiner & Buhrer, 1932. Prog. Plant Prot., 53(3), 563–567.
  13. Chałańska, A., Łabanowski, G., Malewski, T. (2011). Wykrycie węgorka chryzantemowca (Aphelenchoides ritze-mabosi) w nasionach szałwii błyszczącej (Salvia splendens) [Detection of Aphelenchoides ritzemabosi (Schwartz) in scarlet sage (Salvia splendens) seeds]. Prog. Plant Prot., 51(3), 1–5.
  14. Chen, S., Grabau, Z.J. (2018). Biology, ecology and management of plant parasitic nematodes in Minnesota. In: Plant Parasitic nematodes in sustainable agriculture of North America, sustainability in plant and crop protec-tion, Subbotin, S.A. Chitambar J.J. (eds.). Springer, Cham, 125–155. https://doi.org/10.1007/978-3-319-99588-5_6
  15. Chitambar, J.J., Westerdahl, B.B., Subbotin, S.A. (2018). Plant parasitic nematodes in California agriculture. In: Plant parasitic nematodes in sustainable agriculture of North America, sustainability in plant and crop protec-tion, Subbotin, S.A. Chitambar J.J. (eds.). Springer, Cham, 131–192. https://doi.org/10.1007/978-3-319-99585-4_6
  16. Cid del Prado-Vera, I., Franco-Navarro, F., Godinez-Vida, D. (2018). Plant parasitic nematodes and management strategies of major crops in Mexico. In: Plant parasitic nematodes in sustainable agriculture of North America, sustainability in plant and crop protection, Subbotin, S.A. Chitambar J.J. (eds.). Springer, Cham, 31–68. https://doi.org/10.1007/978-3-319-99585-4_2
  17. Consoli, E., Ruthes, A.C., Reinhard, E., Dahlin, P. (2019). First morphological and molecular report of Aphelen-choides blastophthorus on strawberry plants in Switzerland. Plant Dis., 103(11), 2851–2856. https://doi.org/10.1094/PDIS-07-18-1241-RE
  18. Deimi, A.M., Maafi, Z.T., Rius, J.E.P, Castillo, P. (2006). Aphelenchoides subtenuis (Cobb, 1926) Steiner & Buhrer, 1932 (Nematoda: Aphelenchoididae) from Iran with morphological and morphometric characterization. Nema-tology, 8(6), 903–908.
  19. Deimi, A.M., Chitambar, J.J., Maafi, Z.T. (2008). Nematodes associated with flowering ornamental plants in Ma-hallat, Iran. Nematol. Medit., 36, 115–123.
  20. Dobosz, R., Rybarczyk-Mydłowska, K., Winiszewska, G. 2020. Occurrence of Ditylenchus destructor Thorne, 1945 on a sand dune of the Baltic Sea. J. Plant Prot. Res., 60, 31–40. https://doi.org/10.24425/jppr.2020.132206
  21. EPPO. 2017. Diagnostics. PM 7/87 (2). Ditylenchus destructor and Ditylenchus dipsaci. Bull. OEPP/EPPO Bull., 47(3), 401–419.
  22. Gandarilla Basterrechea, H. (2003). Daños producidos por nemátodos foliares en plantas ornamentales de Cuba [Damage caused by foliar nematodes in ornamental plants in Cuba]. Fitosanidad, 7, 63–64.
  23. Gandarilla Basterrechea, H., Fernández Gonzálvez, E. (2002). Registro actualizado de fitonemátodos en plantas ornamentales de Cuba [Updated record of phytonematodes in ornamental plants of Cuba]. Fitosanidad, 6, 9–27.
  24. Goodey, T. (1935). Aphelenchoides hodsoni n. sp., a nematode affecting Narcissus bulbs and leaves. J. Helmin-thol., 13, 167–172. https://doi.org/10.1017/S0022149X0000345X
  25. Green, C.D., Sime, S. (1979). The dispersal of Ditylenchus dipsaci with vegetable seeds. Ann. Appl. Biol., 92, 263–270. https://doi.org/10.1111/j.1744-7348.1979.tb03873.x
  26. Handoo, Z., Kantor, M., Carta, L. (2020). Taxonomy and identification of principal foliar nematode species (Aphe-lenchoides and Litylenchus). Plants, 9, 1490. https://doi.org/10.3390/plants9111490
  27. Hodda, M. (2022). Phylum Nematoda: a classification, catalogue and index of valid genera, with a census of valid species. Zootaxa, 5114, 001–289. https://doi.org/10.11646/zootaxa.5114.1.1
  28. Holterman, M., van der Wurff, A., van den Elsen, S., van Megen, H., Bongers, T., Holovachov, O., Bakker, J., Hel-der, J. (2006). Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades. MBE, 23, 1792–1800. https://doi.org/10.1093/molbev/msl044
  29. Hooper, D.J. Evans. K. (1993). Extraction, identification and control of plant parasitic nematodes. In: Plant parasit-ic nematodes in temperate agriculture, Evans, K., Trudgill, D.L., Webster, J.M. (eds.). CABI Publishing, Walling-ford, 202 pp.
  30. Kantor, M., Handoo, Z., Carta, L., Li, S. (2022a). First report of beech leaf disease caused by Litylenchus crenate mccanni on American beech (Fagus grandifolia) in Virginia. Plant Dis., 206, 1764. https://doi.org/10.1094/PDIS-08-21-1713-PDN
  31. Kantor, M., Handoo, Z., Kantor, C., Carta, L. (2022b). Top ten most important U.S.-regulated and emerging plant-parasitic nematodes. Horticulturae, 8, 208. https://doi.org/10.3390/horticulturae 8030208
  32. Kanzaki, N., Ichihara, Y., Aikawa, T., Ekino, T., Masuya, H. (2019). Litylenchus crenatae n. sp. (Tylenchomorpha: Anguinidae) a leaf gall nematode parasitizing Fagus crenata Blume. Nematology, 21, 5–22.
  33. Kanzaki, N., Shokoohi, E., Fourie, H., Swart, A., Muller, L., Giblin-Davis, R.M. (2018). On the morphology and phy-logeny of Robustodorus Andrássy, 2007 and two ‘Aphelenchoides’ species (Nematoda: Aphelenchoidinae). Nematology, 20, 601–615.
  34. Kaur, H., Attri, R. (2013). Morphological and morphometrical characterization of Meloidogyne incognita from different host plants in four districts of Punjab, India. J. Nematol., 45(2), 122–127.
  35. Kühn J. (1857). Uber das Vorkommen Anguillulen in erkrankten Bluhtenkopfen von Dipsacus fullorum. L. Zeitschrift furWissenschaftliche Zoologie, 9, 129‒137.
  36. Knight, K.W.L., Barber, C.J., Page, G.D. (1997). Plant-parasitic nematodes of New Zealand recorded by host associ-ation. J. Nematol., 29(4S), 640–656.
  37. Kohl, L.M. (2011). Foliar nematodes: a summary of biology and control with a compilation of host range. Plant Health Prog. https://doi.org/10.1094/PHP-2011-1129-01-RV [access: 17.06.2024]
  38. Kusuma, M.D., Supramana, Giyanto. (2020). Phytonematodes community and polyphasic character of Aphelen-choides varicaudatus on garlic plants in Tegal Regency, Central Java. J. Perlindungan Tanam. Indones., 24, 216–223. https://doi.org/10.22146/jpti.49779
  39. MacGuidwin, A.E. (2018). Nematodes important to agriculture in Wisconsin. In: Plant parasitic nematodes in sus-tainable agriculture of North America, sustainability in plant and crop protection, S.A., Subbotin, J.J., Chitambar (eds.). Springer, Cham, 157–180. https://doi.org/10.1007/978-3-319-99588-5_7
  40. Marra, R.E., La Mondia, J.A. (2020). First report of beech leaf disease, caused by the foliar nematode, Litylenchus crenatae mccannii, on American beech (Fagus grandifolia) in Connecticut. Plant Dis., 104, 2527. https://doi.org/10.1094/PDIS-02-20-0442-PDN
  41. Mor, M., Spiegel, Y. (1993). Infection of Narcissus roots by Aphelenchoides subtenuis. J. Nematol., 25(3), 476–479.
  42. Paramonov, A.A. (1964). Principles in Phytonematology. Vol. II. Izdatelstvo “Nauka”, Moskow, CCCP, 443 pp. (in Russian).
  43. Pedroche, N.B., Villaneuva, L.M., De Waele, D. (2013). Plant-parasitic nematodes associated with semi-temperate vegetables in the highlands of Benguet Province, Philippines. Arch. Phytopathol. Plant Prot., 46(3), 278–294. https://doi.org/10.1080/03235408.2012.739928
  44. Pokharel, R.R. (2018). Nematodes in Maryland and Delaware Crops. In: Plant parasitic nematodes in sustainable agriculture of North America, sustainability in plant and crop protection, S.A. Subbotin, J.J. Chitambar (eds.). Springer, Cham, 327–356. https://doi.org/10.1007/978-3-319-99588-5_13
  45. Rathor, Y.S., Tiwari, S.N. (2016). Host affinity of nematode species of the genus Aphelenchoides. IJSR 5, 227–230.
  46. Reed, S.E., Greifenhagen, S., Yu, Q., Hoke, A., Burke, D.J., Carta, L.K., Handoo, Z.A., Kantor, M.R., Koch, J. (2020). Foliar nematode, Litylenchus crenatae ssp. mccannii, population dynamics in leaves and buds of beech leaf disease-affected trees in Canada and the US. Forest Pathol., 50, e12599. https://doi.org/10.1111/efp.12599
  47. Sánchez-Monge, A., Flores, L., Salazar, L., Hockland, S., Bert, W. (2015). An updated list of the plants associated with plant-parasitic Aphelenchoides (Nematoda: Aphelenchoididae) and its implications for plant-parasitism within this genus. Zootaxa, 4013(2), 207–224. http://dx.doi.org/10.11646/zootaxa.4013.2.3
  48. Sharma, R.D., Barbosa Cavalcanti, J.M., Fereira Valentin, J. (2001). Nematόides associatos ao capim-Marandu no estado do Acre [Nematodes associated with Marandu grass in the state of Acre]. Com. Téc. Embrapa Cerrados, Planaltina, 46, 1–4.
  49. Steiner, G., Buhrer, E.M. (1932). Miscellaneous notes on nemic diseases. Plant Dis. Rep., 16, 137.
  50. Stoyanov, D. (1979). The nematode fauna of rice in Bulgaria. Rasteniev"dni Nauki, 16, 115–120. Available at: https://www.cabidigitallibrary.org/doi/full/10.5555/19810884553 [date of access: 11 05 2024].
  51. Szczygieł, A. (1967). Wstępna ocena szkodliwości nicieni z rodzaju Aphelenchoides dla truskawek w Południowej Polsce [Preliminary assessment of the harmfulness of Aphelenchoides for strawberries in Southern Poland]. Pra-ce Inst. Sad., 11, 211–224.
  52. Thorne, G. (1945). Ditylenchus destructor, n. sp., the potato rot nematode, and Ditylenchus dipsaci (Kühn, 1857) Filipjev, 1936, the teasel nematode (Nematoda: Tylenchidae). Proc. Helminthol. Soc. Wash., 12, 27–34.
  53. Thorne, G. (1961). Genera Ditylenchus and Pseudhalenchus. In: Principles in Nematology, Thorne, G. (ed.). McGraw-Hill Book Company, New York–Toronto–London, 553 pp.
  54. van Bezooijen, J. (2006). Methods and techniques for Nematology. Wageningen University, 112 pp.
  55. van Leeuwen, P.J., Trompert, J. (2011). Controlling Aphelenchoides subtenuis nematodes with a hot water treat-ment in Crocus and Allium. Acta Hortic. 886, 273–276. https://doi.org/10.17660/ActaHortic.2011.886.38
  56. Vieira, P., Kantor, M.R., Medina-Mora, C.M., Sakalidis, M.L., Handoo, Z.A. (2023). First report of the beech leaf disease nematode Litylenchus crenatae mccannii (Nematoda: Anguinidae) in Michigan. Plant Dis. 107, 2266.
  57. Vovlas, N., Troccoli, A., Palomares-Rius, J.E., De Luca, F., Liebanas, G., Landa, B.B., Subbotin, S.A., Castillo, P. (2011). Ditylenchus gigas n. sp. parasitizing broad bean: a new stem nematode singled out from the Ditylen-chus dipsaci species complex using a polyphasic approach with molecular phylogeny. Plant Pathol, 60, 762–775. https://doi. org/10.1111/j.1365-3059.2011. 02430.x
  58. Wendt, K.R., Swart, A., Vrain, T.C., Webster, J.M. (1995). Ditylenchus africanus sp. n. from South Africa; a mor-phological and molecular characterization. Fundam. Appl. Nematol., 18, 241–250.
  59. Westphal, A., Chitambar, J.J. , Subbotin, S.A. (2018). Nematodes of agricultural importance in Indiana, Illinois, Iowa, Missouri and Ohio. In: Plant parasitic nematodes in sustainable agriculture of North America, sustainabil-ity in plant and crop protection, Subbotin, S.A., Chitambar, J.J. (eds.). Springer, Cham, 87–107. https://doi.org/10.1007/978-3-319-99588-5_4
  60. Yoder, M., De Ley, I.T., King, I., Mundo-Ocampo, M., Mann, J., Blaxter, M., Poiras, L.P. De Ley. (2006). DESS: a versatile solution for preserving morphology and extractable DNA of nematodes. Nematology, 8, 367–376. https://doi.org/10.1163/156854106778493448
  61. Zasada, I.A., Dandurand, L.M., Gleason, C., Hagerty, C.H., Ingham, R.E. (2018). Plant parasitic nematodes of the Pacific Northwest: Idaho, Oregon and Washington. In: Subbotin, S.A., Chitambar, J.J. (eds.), Plant parasitic nematodes in sustainable agriculture of North America, sustainability in plant and crop protection. Springer, Cham, 211–239. https://doi.org/10.1007/978-3-319-99585-4_8
  62. Zhang, S.L., Liu, G.K., Janssen, T., Zhang, S.S., Xiao, S., Li, S.T., Couvreur, M, Bert, W. (2014). A new stem nema-tode associated with peanut pod rot in China: morphological and molecular characterization of Ditylenchus arachis n. sp. (Nematoda: Anguinidae). Plant Pathol., 63, 1193–1206. http://onlinelibrary.wiley.com/doi/10.1111/ppa.12183/full

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