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ASPHC Baner

Vol. 19 No. 2 (2020)

Articles

EXPRESSION AND CHARACTERISATION OF CUCUMBER FRUIT FLESH THICKNESS-RELATED GENE CSA2M058670.1

DOI: https://doi.org/10.24326/asphc.2020.2.3
Submitted: April 15, 2020
Published: 2020-04-15

Abstract

Bulked segregant analysis combined with specific length amplified fragment sequencing techniques have been applied to determine the fine genetic mapping of fruit flesh thickness-related genes in cucumber. Herein, the Csa2M058670.1 gene was subjected to real time fluorescence quantitative PCR (qRT-PCR) and sequence analysis, indicating a strong correlation with cucumber fruit flesh thickness. Expression and characterisation of the Csa2M058670.1 gene were performed based on previous studies. The results of the fluorescence-based quantitative PCR showed that Csa2M058670.1 was expressed in all organs, but levels were highest in fruit peel, fruit flesh, and female flowers. Furthermore, Csa2M058670.1 expression was induced by abiotic stresses including drought, low temperature, and high salt. Domain analysis revealed that the protein encoded by Csa2M058670.1 possesses an SET (Su(var), Enhancern of zeste CE(z), and Trithorax) domain that may control cell division and differentiation. Therefore, we speculated that Csa2M058670.1 might affect fruit flesh thickness in cucumbers by influencing cell division.

References

  1. Bo, K.L., Ma, Z., Chen, J.F. (2015). Molecular mapping reveals structural rearrange ments and quantitative trait loci underlying traits with local adaptation in semi-wild Xishuangbanna cucumber (Cucumis sativus L. var. xishuangbannanesis Qi et Yuan ). Theo. Appl. Genet., 128, 25–39. DOI: 10.1007/s00122-014-2410-z
  2. Cowan, A.K., Moore-Gordon, C.S., Bertling, I. (1997). Metabolic control of avocado fruit growth (isoprenoid growth regulators and the reaction catalyzed by 3-hydroxy-3-methylglutaryl coenzyme A reductase). Plant Physiol., 114(2), 511–518. DOI: 10.1104/pp.114.2.511
  3. Harada, T., Kurahashi, W., Yanai, M. (2005). Involvement of cell proliferation and cell enlargement in increasing the fruit size of Malus species. Sci. Hortic., 105(4), 447–456.
  4. Higashi, K., Hosoya, K., Ezura, H. (1999). Histological analysis of fruit development between two melon (Cucumis melo L. reticulatus ) genotypes setting a different size of fruit. J. Exp. Bot., 50(339), 1593–1597.
  5. http://www.cbs.dtu.dk/services/TMHMM-2.0/
  6. http://www.ncbi.nlm.nih.gov
  7. https://swissmodel.expasy.org/interactive
  8. https://web.expasy.org/cgi-bin/protparam/protparam
  9. Jullien, A., Munier-Jolain, N.G., Malézieux, E. (2001). Effect of pulp cell number and assimilate availability on dry matter accumulation rate in a banana fruit (Musa sp. AAA group ‘Grande Naine’ (Cavendish subgroup)). Ann. Bot., 88(2), 321–330.
  10. Li, X.Q., Zhao, Y.L., Liu, X.M. (2008). The correlation and path analysis of agronomic characters and yield of tomato in Autumn Greenhouse. Gansu. Agric. Sci. Tech., 9, 8–10.
  11. Li, Y.H., Wen, C.L., Weng, Y.Q. (2013). Fine mapping of the pleiotropic locus B for black spine and orange mature fruit color in cucumber identifies a 50 kb region containing a R2R3-MYB transcription factor. Theor. Appl. Genet., 126, 2187–2196. DOI: 10.1007/s00122-013-2128-3
  12. Li, Y.H., Zhang, Z., Sun, G.M. (2001). Changes in cell number and cell size during pineapple (Ananas comosus L.) fruit development and their relationship with fruit size. Aust. J. Bot., 58(8), 673–678. DOI: 10.1071/BT10225
  13. Liang, X.Q., Du, Y.P., Wang, D.L.,Yang, Y. (2013). The biological of lysine methyltransferase PR-SET7. Hereditas, 35(3), 241–254 [in Chinese]. DOI: 10.3724/sp.j.1005.2013.00241
  14. Liu, H., Wang, T.T., Zhang, J.H. (2015). Cloning and expression analysis of a NAC transcription factor SlNAC80 in tomato. Acta Agric. Bor-Occid. Sin., 30(1), 77–83.
  15. Meng, Y.J., Li, J., Xu, J. (2015). Cloning and expression analysis of the expansion gene Cs EXPb1 in cucumber. Acta Hortic. Sin., 42(4), 679–688 [in Chinese].
  16. Olmstead, J.W., Iezzoni, A.F., Whiting, M.D. (2007). Genotypic differences in sweet cherry fruit size are primarily a function of cell number. J. Am. Soc. Hortic Sci., 132(5), 697–703. DOI: 10.21273/JASHS.132.5.697
  17. Scorzal, R., May, L.G., Purnell, B., Upchurch, B. (1991). Differences in number and area of mesocarp cells between small- and large-fruited peach cultivars. J. Am. Soc. Hortic. Sci., 116(5), 861–864. DOI: 10.21273/JASHS.116.5.861
  18. Song, J.H., Cao, J.S. (2007). Plant SET protion. J. Chin. Cell Biol., 29, 284–288.
  19. Song, M.F., Wei, Q.Z., Fu, W.Y. (2016). Research progress of molecular basis of fruit quality traits in cucurbits. Mol. Plant Breed., 14(11), 3195–3204.
  20. Sun, Y.D., Liang, Y., Wu, J.G. (2010). Correlation analysis on quantitative traits of tomato germplasm resources. China Veget., 6, 74–76.
  21. Wang, L., Wang, M., Shi, Y. (1998). Genetic correlation of major quantitative traits in processed tomato. Acta Agric. Bor-Occid. Sin., 7(1), 32–37.
  22. Wang, Y., He, S.H., Pei, Y. (2015). Transcription character of FaChi1-FaChi4 and their responses to drought stress, exogenous abscisic acid and Botrytis cinerea in strawberry fruit. J. China Agric. Univ., 20(6), 108–116.
  23. Xu, Q., Chen, X.H., Yu, J. (2001). Preliminary study on heritability and genetic correlation of quality characters in pickling cucumber (Cucumis sativus L.), Jiangsu. Agric. Res., 22(4), 18–20.
  24. Xu, X.W., Lu, L., Zhu, B.Y. (2015). QTL mapping of cucumber fruit flesh thickness by SLAF-seq. Sci. Rep., 5, 15829. DOI: 10.1038/srep15829
  25. Xue, H., Tian, F. (2005). Advances in the research of SET gene family. Med. Mol. Biol., 2(3), 190–193.
  26. Yang, X.Q., Zhang, W.W., He, H.L. (2014). Tuberculate fruit gene Tu encodes a C2H2 zinc finger protein that is required for the warty fruit phenotype in cucumber (Cucumis sativus L.). Plant J., 78(6), 1034–1046. DOI: 10.1111/tpj.12531
  27. Yue, S.J., Hu, A., Shao, G.J. (2001). Genetic analysis on fruit mass, pericarp thickness and locule number in tomato. J. South China Agric. Univ., 32(2), 12–15.
  28. Zhang, L.S., Ma, C.G., Ji, Q., Wang, Y.F. (2009). Genome-wide identification, classification and expression analyses of SET domain gene family in Arabidopsis and rice. Hereditas, 31(2), 186–198 [in Chinese]. DOI: 10.3724/sp.j.1005.2009.00186
  29. Zhong, J.X., Luo, Y., Zeng, R.J., Liu, Y., Wu, L.D. (2017). Principal component analysis and comprehensive evaluation of fruit traits of cucumber germplasm resources. Chin. Agric. Sci. Bull., 33(16), 46–52.
  30. Zhu, B.Y., Lu, L., Xu, X.W., Xu, Q. (2016). Genetic analysis of fruit flesh thickness in cucumber. Mol. Plant Breed., 14(7), 1759–1763.

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