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ASPHC Baner

Tom 21 Nr 6 (2022)

Artykuły

In vitro efficacy of herbal plant extracts on some phytopathogenic fungi

DOI: https://doi.org/10.24326/asphc.2022.6.7
Przesłane: 14 lutego 2022
Opublikowane: 2022-12-30

Abstrakt

Crops are subject to yield losses caused by the presence of pests whose species and population diversity are changing with climate warming. The use of agrochemicals is still the most effective method of plant protection against diseases and pests. However, the intensive use of pesticides in some EU countries makes it necessary to search for alternative methods that can be applied in plant protection for consumer and environmental safety. The aim of the study was the laboratory evaluation of the fungistatic effect of extracts of herbal plants occurring in natural state in Poland: horseradish (Armoracia rusticana L.), yarrow (Achillea millefolium L.) and tansy (Tanacetum vulgare L.) on selected polyphagic phytopathogenic fungi (Alternaria alternata, Botrytis cinerea, Colletotrichum coccodes and Fusarium oxysporum). In this study, 5%, 10% and 20% concentrations of herbal water-extract extracts were applied to evaluate their effects on linear growth of fungi and inhibition of their growth relative to control. Total content of polyphenols and flavonoids was assessed in the extracts using spectrophotometry, and their antioxidant activity by applying the synthetic DPPH radical. The plant extracts of tansy and yarrow leaves were characterized by a higher content of polyphenols and flavonoids compared to horseradish leaf extracts; they also had a higher antioxidant activity. Plant extracts inhibited the growth of fungi to a different extent, depending on the species of fungus, type of extract, its concentration and duration of action. The strongest fungistatic effect was recorded for tansy and yarrow extracts, while the weakest for the extract of horseradish leaves. Plant extracts showed the weakest effect against Botrytis cinerea, inhibiting the development of this fungus only during the first days of the experiment. The present research is a preliminary study that will be used in the further to develop a biological preparation for the protection of agricultural and horticultural plants against fungal pathogens.

Bibliografia

  1. Afshari, M., Rahimmalek, M., Miroliaei, M. (2018). Variation in polyphenolic profiles, anti-oxidant and antimicrobial activity of different Achillea species as natural sources of anti-glycative compounds. Chem. Biodivers., 15(8), e1800075. https://doi.org/10.1002/cbdv.201800075 DOI: https://doi.org/10.1002/cbdv.201800075
  2. Agneta, R., Möllers, C., Rivelli, A.R. (2013). Horseradish (Armoracia rusticana), a neglected medical and condiment species with a relevant glucosinolate profile: a review. Genet. Resour. Crop. Evol., 60, 1923–1943. https://doi.org/10.1007/s10722-013-0010-4 DOI: https://doi.org/10.1007/s10722-013-0010-4
  3. Aleksandrowicz-Trzcińska, M., Hallmann E. (2013). Cebula (Allium cepa L.) jako biologicz-ny środek ochrony roślin w szkółkach leśnych. Sylwan, 157(7), 495–505.
  4. Bączek, K.B., Kosakowska, O., Przybył, J.L., Pióro-Jabrucka, E., Costa, R., Mondello, L., Gniewosz, M., Synowiec, A., Węglarz, Z. (2017). Antibacterial and antioxidant activity of essential oils and extracts from costmary (Tanacetum balsamita L.) and tansy (Tanacetum vulgare L.). Ind. Crop. Prod., 102, 154–163. http://dx.doi.org/10.1016/j.indcrop.2017.03.009 DOI: https://doi.org/10.1016/j.indcrop.2017.03.009
  5. Bączek, K., Kosakowska, O., Przybył, J.L., Kuźma, P., Ejdys, M., Obiedziński, M., Węglarz, Z. (2015). Intraspecific variability of yarrow (Achillea millefolium L.) in respect of develop-mental and chemical traits. Herba Pol., 61(3), 37–52. https://doi.org/10.1515/hepo-2015-0021 DOI: https://doi.org/10.1515/hepo-2015-0021
  6. Biller, E., Waszkiewicz-Robak, B., Obiedziński, M., Kalinowski, K. (2019). Antioxidant properties of horseradish (Armoracia rusticana) – Pilot studies. Pol. J. Appl. Sci., 4(2), 55–59. https://doi.org/10.34668/PJAS.2018.4.2.03
  7. Brand-Williams, W., Cuvelier, M.E., Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT – Food Sci. Technol., 28, 25–30. https://doi.org/10.1016/S0023-6438(95)80008-5 DOI: https://doi.org/10.1016/S0023-6438(95)80008-5
  8. Burgieł, Z.J., Moliszewska, E.B. (1999). Fungistatyczna aktywność wybranych wyciągów roślinnych. Chem. Inż. Ekol., 6(2–3), 157–164.
  9. Candan, F., Unlu, M., Tepe, B., Daferera, D., Polissiou, M., Sökmen, A., Akpulat, H.A. (2003). Antioxidant and antimicrobial activity of the essential oil and methanol extracts of Achillea millefolium subsp. millefolium Afan. (Asteraceae). J. Ethnopharmacol., 87(2–3), 215–220. https://doi.org/10.1016/s0378-8741(03)00149-1 DOI: https://doi.org/10.1016/S0378-8741(03)00149-1
  10. Coté, H., Boucher, M.A., Pichette, A., Legault, J. (2017). Anti-inflammatory, antioxidant, antibiotic, and cytotoxic activities of Tanacetum vulgare L. essential oil and its constituents. Medicines, 4(2), 34. https://doi.org/10.3390/medicines4020034 DOI: https://doi.org/10.3390/medicines4020034
  11. Dekić, M.S., Radulović, N.S., Stojanović, N.M., Randjelović, P.J., Stojanović-Radić, Z.Z., Najman, S., Stojanović, S. (2017). Spasmolytic, antimicrobial and cytotoxic activities of 5-phenylpentyl isothiocyanate, a new glucosinolate autolysis product from horseradish (Armo-racia rusticana P. Gaertn., B. Mey. & Scherb., Brassicaceae). Food Chem., 1(232), 329–339. https://doi.org/10.1016/j.foodchem.2017.03.150 DOI: https://doi.org/10.1016/j.foodchem.2017.03.150
  12. Devrnja, N., Anđelković, B., Aranđelović, S., Radulović, S., Soković, M., Krstić-Milošević, D., Ristić, M., Ćalić, D. (2017). Comparative studies on the antimicrobial and cytotoxic activities of Tanacetum vulgare L. essential oil and methanol extracts. S. Afr. J. Bot., 111, 212–221. https://doi.org/10.1016/j.sajb.2017.03.028 DOI: https://doi.org/10.1016/j.sajb.2017.03.028
  13. Fierascu, I., Ungureanu, C., Avramescu, S.M., Fierascu, R.C., Ortan, A., Soare, L.C., Paunes-cu, A. (2015). In vitro antioxidant and antifungal properties of Achillea millefolium L. Rom. Biotech. Lett., 20, 10626–10636. https://www.rombio.eu/rbl4vol20/9.pdf
  14. Georgieva, L., Gadjalova, A., Mihaylova, D., Pavlov, A. (2015). Achillea millefolium L. – phytochemical profile and in vitro antioxidant activity. Int. Food Res. J., 22(4), 1347–1352.
  15. Giorgi, A., Bononi, M., Tateo, F., Cocucci, M. (2005). Yarrow (Achillea millefolium L.) growth at different altitudes in Central Italian Alps: biomass yield, oil content and quality. J. Herbs Spices Med. Plants, 11(3), 47–58. https://doi.org/10.1300/J044v11n03_05 DOI: https://doi.org/10.1300/J044v11n03_05
  16. Grzyb, A., Waraczewska, Z., Niewiadomska, A., Wolna-Maruwka, A. (2019). Czym są bio-preparaty i jakie jest ich zastosowanie? Nauka Przyr. Technol., 13(2), 65–67. http://dx.doi.org/10.17306/J.NPT.00275
  17. Hać-Szymańczuk, E., Lipińska, E., Grzegrzółka, O. (2012). Ocena aktywności przeciwbakte-ryjnej oregano (Origanum vulgare L.). Bromat. Chem. Toksykol., 45(3), 308–314.
  18. Hafner, M., Raimondi, P.P. (2020). Priorities and challenges of the EU energy transition: From the European Green Package to the new Green Deal. Russ. J. Econom., 6, 374–389. https://doi.org/10.32609/j.ruje.6.55375 DOI: https://doi.org/10.32609/j.ruje.6.55375
  19. Horoszkiewicz-Janka, J., Jajor, E. (2006). Wpływ zaprawiania nasion na zdrowotność roślin jęczmienia, pszenicy i rzepaku w początkowych fazach rozwoju. J. Res. Appl. Agric. Eng., 51(2), 47–53.
  20. Ivănescu, B, Tuchiluș, C., Corciovă, A., Lungu, C., Mihai, C.T., Gheldiu, A.M., Vlase, L. (2018). Antioxidant, antimicrobial and cytotoxic activity of Tanacetum vulgare, Tanacetum corymbosum and Tanacetum macrophyllum extracts. Farmacia, 66(2), 282–288.
  21. Jafernik, K., Szopa, A., Ekiert, H. (2019). Pelargonia przylądkowa (afrykańska) (Pelargonium sidoides), chrzan pospolity (Armoracia rusticana) oraz nasturcja większa (Tropaeolum majus) – skład chemiczny, aktywność biologiczna oraz znaczenie w fitoterapii. Lek Pol., 29(11), 342–343.
  22. Jamiołkowska, A. (2013). Preparaty biotechniczne i biologiczne w ochronie papryki słodkiej (Capsicum annuum L.) przed grzybami chorobotwórczymi i indukowaniu reakcji obronnych roślin. Rozprawy Naukowe UP w Lublinie, 379, pp. 117.
  23. Jamiołkowska, A. (2020). Natural compounds as elicitors of plant resistance against diseases and new biocontrol strategies. Agronomy, 10(2) 173. https://doi.org/10.3390/agronomy10020173 DOI: https://doi.org/10.3390/agronomy10020173
  24. Jamiołkowska, A., Kowalski, R. (2012). Laboratory effect of Silphium perfoliatum L. on the growth of tested fungi. Acta Sci. Pol., Hortorum Cultus, 11(3), 43–55.
  25. Jamiołkowska, A., Kopacki, M. (2019). Natural compounds against plant pests and patho-gens. In: Egbuna, Ch., Sawicka, B. (eds.), Natural remedies for pest, disease and weed con-trol.Elsevier Academic Press, 55–63. https://doi.org/10.1016/B978-0-12-819304-4.00005-1 DOI: https://doi.org/10.1016/B978-0-12-819304-4.00005-1
  26. Jovanović, A., Petrović, P., Đorđević, V., Zdunić, G., Šavikin, K., Bugarski, B. (2017). Poly-phenols extraction from plant sources. Lek. Sirov., 37, 45–49. http://dx.doi.org/10.5937/leksir1737045J DOI: https://doi.org/10.5937/leksir1737045J
  27. Kaczorová, D., Karalija, E., Dahija, S., Bešta-Gajević, R., Parić, A., Ćavar Zeljković, S. (2021). Influence of Extraction Solvent on the Phenolic Profile and Bioactivity of Two Achillea Species. Molecules, 26(6), 1601. https://doi.org/10.3390/molecules26061601 DOI: https://doi.org/10.3390/molecules26061601
  28. Karadeniz, F., Burdurlu, H.S., Koca, N., Soyer, Y. (2005). Antioxidant activity of selected fruits and vegetables grown in Turkey. Turk. J. Agric. For., 29(4), 297–303.
  29. Kaser, S., Celik, S., Tourkoglu, S., Yilmaz, Ö., Tourkoglu, I. (2013). Antioxidant activity, total phenolic and flavonoid content of water and ethanol extracts from Achillea millefolium L. Turk. J. Pharm. Sci., 10(3), 385–392.
  30. Krzepiłko, A., Kordowska-Wiater, M., Sosnowska, B., Pytka, M. (2020). Funkcje w roślinach oraz działanie przeciwdrobnoustrojowe wybranych grup metabolitów wtórnych. In: Krzepiłko, A., Kordowska-Wiater, M., Sosnowska, B., Pytka, M., Oddziaływanie ekstraktów roślinnych na drobnoustroje. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie, 53–60. DOI: https://doi.org/10.24326/mon.2020.4
  31. Lorens, E., Garcia-Agustin, P., Lapeña L. (2017). Advances in induced resistance by natural compounds: towards new options for woody crop protection. Sci. Agric., 74(1), 90–100. https://doi.org/10.1590/1678-992X-2016-0012 DOI: https://doi.org/10.1590/1678-992x-2016-0012
  32. Lucas, G.C., Alves, E., Pereira, R.B., Perina, F.J., Souza, R.M. (2012). Antibacterial activity of essential oils on Xanthomonas vesicatoria and control of bacterial spot in tomato. Pesqui. Agropecu. Bras., 47, 351–359. https://doi.org/10.1590/s0100-204x2012000300006 DOI: https://doi.org/10.1590/S0100-204X2012000300006
  33. Mitich, L. (1992). Tansy. Weed Technol., 6(1), 242–244. https://doi.org/10.1017/S0890037X00034643 DOI: https://doi.org/10.1017/S0890037X00034643
  34. Montanarella, L., Panagos, P. (2021). The relevance of sustainable soil management within European Green Deal. Land Use Policy, 100, 1–2. https://doi.org/10.1016/j.landusepol.2020.104950 DOI: https://doi.org/10.1016/j.landusepol.2020.104950
  35. Mot, C.A., Lupitu, A.I., Bungau, S., Iovan, C., Copolovici, D.M., Purza, L., Melinte, E.C., Copolovici, L. (2018). Composition and antioxidant activity of aqueous extracts obtained from herb of Tansy (Tanacetum vulgare L.). Rev. Chim., 69(5), 1041–1044. https://doi.org/10.37358/RC.18.5.6257 DOI: https://doi.org/10.37358/RC.18.5.6257
  36. Nowak, K., Ogonowski, J., Szulc, K. (2010). Application and characteristics of Achillea
  37. millefolium and its oil. Chemik, 64(2), 103–110.
  38. Nxumalo, K.A., Aremu, A.O., Fawole, O.A. (2021). Potential of medicinal plants extracts as an alternative to synthetic chemicals in postharvest protection and preservation of horticultural crops: a review. Sustainability, 13(11), 5897. https://doi.org/10.3390/su13115897 DOI: https://doi.org/10.3390/su13115897
  39. Petrović, V., Četojević-Simin, D., Milanović, M., Vulić, J., Milić, N. (2021). Polyphenol rich horseradish root extracts and juice: in vitro antitumor activity and mechanism of action. Vojnosanit. Pregl., 78(7), 745–754. https://doi.org/10.2298/VSP190212123P DOI: https://doi.org/10.2298/VSP190212123P
  40. Piekutowska, M. (2017). Potencjał naturalnych preparatów pochodzenia roślinnego dla po-prawy zdrowotności i żywotności materiału siewnego roślin rolniczych. Probl. Small Agric. Hold., 3, 43–59. http://dx.doi.org/10.15576/PDGR/2017.3.43 DOI: https://doi.org/10.15576/PDGR/2017.3.43
  41. Plaszkó, T., Szűcs, Z., Kállai, Z., Csoma, H., Vasas, G., Gonda, S. (2020). Volatile Organic Compounds (VOCs) of Endophytic Fungi Growing on Extracts of the Host, Horseradish (Ar-moracia rusticana). Metabolites, 10(11), 451. https://doi.org/10.3390/metabo10110451 DOI: https://doi.org/10.3390/metabo10110451
  42. Pralińska, M., Jaśkiewicz, J., Rackiewicz, I. (2020). Wyzwania dla rolnictwa związane ze strategią Europejski Zielony Ład w okresie pandemii. Probl.World Agric./Probl. Roln. Świat., 20(2), 22–36. https://doi.org/10.22630/PRS.2020.20.2.10 DOI: https://doi.org/10.22630/PRS.2020.20.2.10
  43. Rahimmalek, M., Tabatabaei, B.E.S., Etemadi, N., Goli, S.A.H., Arzani, A., Zeinali, H. (2009). Essential oil variation among and within six Achillea species transferred from differ-ent ecological regions in Iran to the field conditions. Ind. Crop. Prod., 29(2–3), 348–355. https://doi.org/10.1016/j.indcrop.2008.07.001 DOI: https://doi.org/10.1016/j.indcrop.2008.07.001
  44. Roccioni, L., Orzali, L. (2011). Activity of tea tree (Melaleuca alternifolia, Cheel) and thyme (Thymus vulgaris, Linnaeus.) essential oils against some pathogenic seed borne fungi. J. Essent. Oil Res., 23(6), 43–47. https://doi.org/10.1080/10412905.2011.9712280 DOI: https://doi.org/10.1080/10412905.2011.9712280
  45. SAS 9.1 (2004). SAS/STAT® 9.1. User’s Guide. SAS Institute Inc., Cary, NC, USA.
  46. Singleton, V.L., Rossi, J.A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Viticult., 16(3), 144–158.
  47. Stojanović, G., Radulović, N., Hashimoto, T., Palić, R. (2005). In vitro antimicrobial activity of extracts of four Achillea species: The composition of Achillea clavennae L. (Asteraceae) extract. J. Ethnopharmacol., 101(1–3), 185–190. https://doi.org/10.1016/j.jep.2005.04.026 DOI: https://doi.org/10.1016/j.jep.2005.04.026
  48. Tedeschi, P., Leis, M., Pezzi, M., Civolani, S., Maietti, A., Brandolini, V. (2011). Insecticidal activity and fungitoxicity of plant extracts and components of horseradish (Armoracia rusticana) and garlic (Allium sativum). J. Environ. Sci. Heal. B, 46(6), 486–490. https://doi.org/10.1080/03601234.2011.583868
  49. Vilhelmova, N., Simeonova, L., Nikolova, N., Pavlova, E., Gospodinova, Z., Antov, G., Galabov, A., Nikolova, I. (2020). Antiviral, cytotoxic and antioxidant effects of Tanacetum vulgare L. crude extract in vitro. Folia Med., 62, 172–179. https://doi.org/10.3897/folmed.62.e49370 DOI: https://doi.org/10.3897/folmed.62.e49370
  50. Vitalini, S., Beretta, G., Iriti, M., Orsenigo, S., Basilico, N., Dall’Acqua, S., Iorizzi, M., Fico, G. (2011). Phenolic compounds from Achillea millefolium L. and their bioactivity. Acta Bio-chim. Pol., 58(2), 203–209. DOI: https://doi.org/10.18388/abp.2011_2266
  51. Wrzaszcz, W., Prandecki, K. (2020). Rolnictwo a Europejski Zielony Ład. Zag. Ekon. Roln./Probl. Agric. Econom., (4),365, 156–179. https://doi.org/10.30858/zer/131841 DOI: https://doi.org/10.30858/zer/131841
  52. Wyrostek, J., Kowalski, R., (2022). Effect of ultrasound and fragmentation of the raw material on the extraction of phenolic compounds from peppermint leaves and black tea. Przem. Chem., 101(2), 928–933.
  53. Wyszkowska-Kolatko, M., Koczurkiewicz, P., Wójcik, K., Pękala, E. (2015). Rośliny leczni-cze w terapii chorób skóry. Postępy Fitoter., 3, 184–192, 2015.
  54. Zawiślak, G., Nurzyńska-Wierdak, R. (2017). Plon surowca uprawianych oraz dziko rosną-cych roślin krwawnika pospolitego (Achillea millefolium L.) i wrotyczu pospolitego (Tanacetum vulgare L.). Ann. Hortic., 27(2), 27–28. https://doi.org/10.24326/ah.2017.2.3 DOI: https://doi.org/10.24326/ah.2017.2.3

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