TISSUE CULTURE MULTIPLICATION OF Paphiopedilum insigne DEPENDING ON THE MEDIUM TYPE, GROWTH REGULATORS AND NATURAL SUPPLEMENTS

Monika Poniewozik

Subdepartment of Ornamental Plants and Dendrology, Institute of Horticultural Production, University of Life Sciences in Lublin, Poland
http://orcid.org/0000-0001-7637-2626

Paweł Szot

Subdepartment of Ornamental Plants and Dendrology, Institute of Horticultural Production, University of Life Sciences in Lublin, Poland
https://orcid.org/0000-0002-9138-7305

Marzena Parzymies

Subdepartment of Ornamental Plants and Dendrology, Institute of Horticultural Production, University of Life Sciences in Lublin, Poland
http://orcid.org/0000-0002-4835-3804


Abstract

Paphiopedilum is an ornamental orchid used mainly for interior decoration. As the division of plants is uneconomical, a fast method of propagation is needed. The aim of this study was to evaluate the influence of medium type (MS or VW), growth regulators, i.e. BA, KIN, TDZ used separately or in combinations and natural additives, i.e. coconut water, banana pulp, casein hydrolysate, on Paphiopedilum insigne plantlets grown in vitro. It was found out that BA in concentration of 0.5 mg·dm–3 allowed to obtain the highest multiplication rate (2.92), however the use of KIN in concentration of 1 mg·dm–3 resulted in formation of bigger and higher quality plantlets, It is possible to replace cytokinins with other biologically active substance, such as banana pulp. The 1/2 MS medium might be used for Paphiopedilum insigne tissue culture, as there was no difference in terms of multiplication rate and the obtained plantlets were of better quality, especially that it is cheaper and easier to prepare.

Keywords:

biological supplements, lady slipper, micropropagation, orchid, cytokinins

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Published
2021-08-31



Monika Poniewozik 
Subdepartment of Ornamental Plants and Dendrology, Institute of Horticultural Production, University of Life Sciences in Lublin, Poland http://orcid.org/0000-0001-7637-2626
Paweł Szot 
Subdepartment of Ornamental Plants and Dendrology, Institute of Horticultural Production, University of Life Sciences in Lublin, Poland https://orcid.org/0000-0002-9138-7305
Marzena Parzymies 
Subdepartment of Ornamental Plants and Dendrology, Institute of Horticultural Production, University of Life Sciences in Lublin, Poland http://orcid.org/0000-0002-4835-3804



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