Skip to main navigation menu Skip to main content Skip to site footer
ASPHC Baner

Vol. 20 No. 4 (2021)

Articles

TISSUE CULTURE MULTIPLICATION OF Paphiopedilum insigne DEPENDING ON THE MEDIUM TYPE, GROWTH REGULATORS AND NATURAL SUPPLEMENTS

DOI: https://doi.org/10.24326/asphc.2021.4.11
Submitted: June 30, 2020
Published: 2021-08-31

Abstract

Paphiopedilum is an ornamental orchid used mainly for interior decoration. As the division of plants is uneconomical, a fast method of propagation is needed. The aim of this study was to evaluate the influence of medium type (MS or VW), growth regulators, i.e. BA, KIN, TDZ used separately or in combinations and natural additives, i.e. coconut water, banana pulp, casein hydrolysate, on Paphiopedilum insigne plantlets grown in vitro. It was found out that BA in concentration of 0.5 mg·dm–3 allowed to obtain the highest multiplication rate (2.92), however the use of KIN in concentration of 1 mg·dm–3 resulted in formation of bigger and higher quality plantlets, It is possible to replace cytokinins with other biologically active substance, such as banana pulp. The 1/2 MS medium might be used for Paphiopedilum insigne tissue culture, as there was no difference in terms of multiplication rate and the obtained plantlets were of better quality, especially that it is cheaper and easier to prepare.

References

  1. Aktar, S., Nasiruddin, K.M., Hossain, K. (2008). Effects of different media and organic additives interaction on in vitro regeneration of Dendrobium orchid. J. Agric. Rural Dev., 6(1), 69–74. DOI:10.3329/jard.v6i1.1659
  2. Asghar, S., Ahmad, T., Hafiz, I.A., Yaseen, M. (2011). In vitro propagation of orchid (Dendrobium nobile) var. Emma White (!). Afr. J. Biotechnol., 10(16), 3097–3103. https://doi.org/10.5897/AJB10.401
  3. Bektaṣ, E., Sökmen, A. (2016). In vitro seed germination, plantlet growth, tuberization, and synthetic seed production of Serapias vomeracea (Burm. f.) Briq. Turk. J. Bot., 40(6), 584–594. https://doi.org/10.3906/bot-1512-13
  4. Chang, W.C., Chang, J.T., Chun Chen, Y., Lin, Y.H., Su, Y.H., Chen, T.Y, Tseng, M.C, Kuo, H.L., Wu, I.F, Chueh, C.M. (2005). In vitro morphogenesis of five orchids. Acta Hortic., 692, 115–118. https://doi.org/10.17660/ActaHortic.2005.692.14
  5. Chugh, S., Guha, S., Rao, I.U. (2009). Micropropagation of orchids: a review on the potential of different explants. Sci. Hortic., 122(4), 507–520. DOI:10.1016/j.scienta.2009.07.016
  6. Gnasekaran, P., Rathinam, X., Sinniah, U.R., Subramaniam, S. (2010). A study on the use of organic additives on the protocorm-like bodies (PLBs) growth of Phalaenopsis violacea orchid. J. Phytol., 2(1), 029–033.
  7. Hong, P.I., Chen, J.T., Chang, W.C. (2008). Plant regeneration via protocorm-like body formation and shoot multiplication from seed-derived callus of a maudiae type slipper orchid. Acta Physiol. Plant., 30(5), 755–759. DOI:10.1007/s11738-008-0158-2
  8. Jitsopakul, N., Thammasiri, K., Ishikawa, K., Wannajuk, M., Sangthong, P., Natapintu, S. (2013). Efficient adventitious shoot regeneration from shoot tip culture of Vanda coerulea, a Thai orchid. Sci. Asia, 39(5), 449–455. https://doi.org/10.2306/scienceasia1513-1874.2013.39.449
  9. Ket, N.V., Hahn, E.J., Park, S.Y., Chakrabarty, D., Paek, K.Y., (2004). Micropropagation of an endangered orchid Anoectochilus formosanus. Biol. Plant., 48(3), 339–344. https://doi.org/10.1023/B:BIOP.0000041084.77832.11
  10. Lo, S.F., Nalawade, S.M., Kuo, C.L., Chen, C.L., Tsay, H.S. (2004). Asymbiotic germination of immature seeds, plantlet development and ex vitro establishment of plants of Dendrobium tosaense makino – a medicinally improrant orchid. In Vitro Cell. Dev. Biol. Plant, 40(5), 528–535. https://doi.org/10.1079/IVP2004571
  11. Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with Tobacco tissue cultures. Physiol. Plant., 15, 473–479. DOI:10.1111/j.1399-3054.1962.tb08052.x
  12. Naik, S.K., Usha Bharathi, T., Barman, D., Rampal, De, L.C., Medhi, R.P. (2010). Basics of orchid nutrition. Mini Mission 1, Technical Bulletin 5. National Research Centre for Orchids (Indian Council of Agricultural Research), Sikkim, India.
  13. Nambiar, N., Tee, C.S., Maziah, M. (2012). Effects of organic additives and different carbohydrate sources on proliferation of protocorm-like bodies in Dendrobium Alya Pink. Plant Omics J., 5(1), 10–18.
  14. Ng, C.Y., Saleh, N.M. (2011) In vitro propagation of Paphiopedilum orchid through formation of protocorm-like bodies. Plant Cell Tiss. Org. Cult., 105, 193. DOI:10.1007/s11240-010-9851-0
  15. Nisayan, E., Thepsithar, C., Thongpukdee, A. (2010). Multiple shoot induction from seed derived shoots of Paphiopedilum. I International Orchid Symposium 878, 199–204. https://doi.org/10.17660/ActaHortic.2010.878.23
  16. Oszkinis, K. (2004). Storczyki (Orchids). Warsaw, PWRiL.
  17. Parthibhan, S., Rao, M.V., Kumar, T.S. (2015). In vitro regeneration from protocorms in Dendrobium aqueum Lindley – An imperiled orchid. J. Genet. Engineer. Biotechnol., 13(2), 227–233. DOI:10.1016/j.jgeb.2015.07.001
  18. Rogozińska, J.H. (1969). Cytokininy i ich znaczenie w procesach fizjologicznych roślin. Wiad. Bot., 13(4), 259–273.
  19. Roy, J., Banerjee, N. (2002). Rhizome and shoot development during in vitro propagation of Geodorum densiflorum (Lam.) Schltr. Sci. Hortic., 94, 181–192. https://doi.org/10.1016/S0304-4238(01)00373-9
  20. Sinha, P., Roy, S.K. (2004). Regeneration of an indigenous orchid, Vanda teres (Roxb.) Lindl. through in vitro culture. Plant Tiss. Cult., 14, 55–61.
  21. Talukder, S.K., Nasiruddin, K.M., Yasmin, S., Hassan, L., Begum, R. (2003). Shoot proliferation of Dendrobium Orchid with BAP and NAA. J. Biol. Sci., 3(11), 1058–1062.
  22. Warghat, A.R., Bajpai, P.K., Srivastava, R.B., Chaurasia, .P., Chauhan, R.S., Sood, H. (2014). In vitro protocorm development and mass multiplication of an endangered orchid, Dactyloriza hatagirea. Turk. J. Bot., 38, 737–746. https://doi.org/10.3906/bot-1308-48
  23. Wattanawikkit, P., Bunn, E., Chayanarit, K., Tantiwiwat, S. (2011). Effect of cytokinins (BAP and TDZ) and auxin (2, 4-D) on growth and development of Paphiopedilum callosum. Nat. Sci., 45, 12–19.
  24. Vacin, E., Went, F. (1949). Some pH changes in nutrient solution. Bot. Gard. Conserv. News. 110, 605–613. https://doi.org/10.1086/335561
  25. Zeng, S., Huang, W., Wu, K., Zhang, J., Teixeria da Silva, J., Duan, J. (2016). In vitro propagation of Paphiopedilum orchids. Crit. Rev. Biotechnol., 36, 521–534. https://doi.org/10.3109/07388551.2014.993585
  26. Zeng, S., Wu, K., Teixeira da Silva, J., Zhang, J., Chen, Z., Xia, N., Duan, J. (2012). Asymbiotic seed germination, seedling development and reintroduction of Paphiopedilum wardii Sumerh., an endangered terrestial orchid. Sci. Hort., 138, 198–209. https://doi.org/10.1016/j.scienta.2012.02.026
  27. Zhang, Y., Lee, Y.I., Deng, L., Zhao, S. (2013). Asymbiotic germination of immature seeds and the seedling development of Cypripedium macranthos Sw., an endangered lady’s slipper orchid. Sci. Hortic., 164, 130–136. https://doi.org/10.1016/j.scienta.2013.08.006

Downloads

Download data is not yet available.

Most read articles by the same author(s)

1 2 > >> 

Similar Articles

1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.